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The map label for this gene is adaA [H]

Identifier: 220904431

GI number: 220904431

Start: 1369264

End: 1369836

Strand: Reverse

Name: adaA [H]

Synonym: Ddes_1161

Alternate gene names: 220904431

Gene position: 1369836-1369264 (Counterclockwise)

Preceding gene: 220904432

Following gene: 220904430

Centisome position: 47.67

GC content: 53.93

Gene sequence:

>573_bases
ATGAAAAGACTTTCAGAGGCGACCATGTGGCAGGCGCTGCTTGACTGCGACCCGTCTTATGACGGACAATTTTTTTATGC
GGTAAAAACCGTGGGCGCATACTGCCGCCCTTCTTGCAAGTCGCGGGCACCGCTGCGAAAAAATGTTTTATACTTCCAGA
CAGCGGCCGAGGCGGAACAGGCCGGGCTGCGCCCTTGCAAGCGTTGCCGTCCTGATCTGGAAAAATACAACCCTGCGGCG
CGCCTTGCCGGACAGGCCAAAAAGCTCATCGACGGCCATTTCGACAAACGCGCCCGCCTGCAAGCAGAACTCAAGCATCT
TGGTATTTCGCAAAGCCATCTGGCGGTTGTTTTTCGGCAACACTATGGCCTGACGCCAGTACAATACCTTGGTCATGTCC
GAGAACGGCGGGCTTGCGCGCTGCTGGAAAACACAAGCATGCCCATCACCGACATTGCCGCTACCATCGGCTTTGAAAGC
CTGGCTTCATTTTATGCGTTTTTCAAAAAGCAGACAGGCACAAGCCCCGCGAAATTCCGTCAACAAGCACATTGTTCAGT
AATCGCAGATTGA

Upstream 100 bases:

>100_bases
AAAGTATGGTTTTGCCCTGAAGGCATATAGGAATTGCATCTTGAAAACAGATAAGACGGATGCGTATGCCTGTGTTATAT
CGCCATCGATGAGGTTTTTC

Downstream 100 bases:

>100_bases
CGCACTTGCTCACATTACCGCAACAATACCTGAAAAGGCATACTGCCTGTGACAATGCGCCGACAATTGGAGTTACACCA
TGAAAAATTTATGGTTTTAC

Product: AraC family transcriptional regulator

Products: DNA; protein S-methyl-L-cysteine

Alternate protein names: NA

Number of amino acids: Translated: 190; Mature: 190

Protein sequence:

>190_residues
MKRLSEATMWQALLDCDPSYDGQFFYAVKTVGAYCRPSCKSRAPLRKNVLYFQTAAEAEQAGLRPCKRCRPDLEKYNPAA
RLAGQAKKLIDGHFDKRARLQAELKHLGISQSHLAVVFRQHYGLTPVQYLGHVRERRACALLENTSMPITDIAATIGFES
LASFYAFFKKQTGTSPAKFRQQAHCSVIAD

Sequences:

>Translated_190_residues
MKRLSEATMWQALLDCDPSYDGQFFYAVKTVGAYCRPSCKSRAPLRKNVLYFQTAAEAEQAGLRPCKRCRPDLEKYNPAA
RLAGQAKKLIDGHFDKRARLQAELKHLGISQSHLAVVFRQHYGLTPVQYLGHVRERRACALLENTSMPITDIAATIGFES
LASFYAFFKKQTGTSPAKFRQQAHCSVIAD
>Mature_190_residues
MKRLSEATMWQALLDCDPSYDGQFFYAVKTVGAYCRPSCKSRAPLRKNVLYFQTAAEAEQAGLRPCKRCRPDLEKYNPAA
RLAGQAKKLIDGHFDKRARLQAELKHLGISQSHLAVVFRQHYGLTPVQYLGHVRERRACALLENTSMPITDIAATIGFES
LASFYAFFKKQTGTSPAKFRQQAHCSVIAD

Specific function: Methylphosphotriester-DNA alkyltransferase is one of two genes required for the adaptive response of bacteria to alkylating agents. It accepts a methyl group from methylphospho-triesters and then acts as a transcriptional activator of the ada operon [H]

COG id: COG2169

COG function: function code F; Adenosine deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788542, Length=185, Percent_Identity=34.0540540540541, Blast_Score=103, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR016220 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF00165 HTH_AraC [H]

EC number: 2.1.1.63

Molecular weight: Translated: 21361; Mature: 21361

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLSEATMWQALLDCDPSYDGQFFYAVKTVGAYCRPSCKSRAPLRKNVLYFQTAAEAEQ
CCCHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHCCCCCCCCCCHHHCEEEEHHHHHHHH
AGLRPCKRCRPDLEKYNPAARLAGQAKKLIDGHFDKRARLQAELKHLGISQSHLAVVFRQ
HCCCHHHHCCCCHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHH
HYGLTPVQYLGHVRERRACALLENTSMPITDIAATIGFESLASFYAFFKKQTGTSPAKFR
HCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
QQAHCSVIAD
HHHCHHHCCC
>Mature Secondary Structure
MKRLSEATMWQALLDCDPSYDGQFFYAVKTVGAYCRPSCKSRAPLRKNVLYFQTAAEAEQ
CCCHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHCCCCCCCCCCHHHCEEEEHHHHHHHH
AGLRPCKRCRPDLEKYNPAARLAGQAKKLIDGHFDKRARLQAELKHLGISQSHLAVVFRQ
HCCCHHHHCCCCHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHH
HYGLTPVQYLGHVRERRACALLENTSMPITDIAATIGFESLASFYAFFKKQTGTSPAKFR
HCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
QQAHCSVIAD
HHHCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA containing 6-O-methylguanine; protein L-cysteine

Specific reaction: DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine

General reaction: Methyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2120677; 9384377; 1744039 [H]