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The map label for this gene is ogt [H]

Identifier: 220904430

GI number: 220904430

Start: 1368684

End: 1369184

Strand: Reverse

Name: ogt [H]

Synonym: Ddes_1160

Alternate gene names: 220904430

Gene position: 1369184-1368684 (Counterclockwise)

Preceding gene: 220904431

Following gene: 220904429

Centisome position: 47.65

GC content: 51.5

Gene sequence:

>501_bases
ATGAAAAATTTATGGTTTTACACCTGCCCCATCGGCAAGATCGGCATAGTTGAAAAAAATGGAGCCATCTGCCAGGTGTT
TTTCAGTACAGAAGCTCCACCTGACGGCTTTACGCTACAAAAAAGCCCGGTTATCGAAAGAGCGGAACATCAGCTTACGG
AATATTTCGCAGGTGATAGAAAAGAATTTGACTTACCCCTGGACCTCGGCGGCACGGAATTTCAGGCAACGGTATGGCAG
GCGCTACAGACCATTCCAGCCGGAGAAACGCGCAGCTACAAAGATGTGGCCGTCATGATCGGCAAGCCCGGCGCCAGTCG
CGCTGTGGGCATGGCCAACAACCGCAACCCGGTTGCCATCATTGTTCCCTGCCACCGTGTCATCGGGCATGACGGCAGCC
TTACCGGATATGCGGGTGGCCTTGCCATCAAGCAATACCTGCTTGACCATGAAAAAATGTGGCAACCAGAGAGAATCCAT
GCCCAATACACATTATTTTGA

Upstream 100 bases:

>100_bases
TGTTCAGTAATCGCAGATTGACGCACTTGCTCACATTACCGCAACAATACCTGAAAAGGCATACTGCCTGTGACAATGCG
CCGACAATTGGAGTTACACC

Downstream 100 bases:

>100_bases
ATATGGCGAAAAAGAAAAATCATGGCTTAAGTCGCGCGATCCGGTGCTGGCCGCCGCTATGGAAGAAATCGGACACATCC
GGCGCGAAGTTACGCCGGAC

Product: methylated-DNA/protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 166; Mature: 166

Protein sequence:

>166_residues
MKNLWFYTCPIGKIGIVEKNGAICQVFFSTEAPPDGFTLQKSPVIERAEHQLTEYFAGDRKEFDLPLDLGGTEFQATVWQ
ALQTIPAGETRSYKDVAVMIGKPGASRAVGMANNRNPVAIIVPCHRVIGHDGSLTGYAGGLAIKQYLLDHEKMWQPERIH
AQYTLF

Sequences:

>Translated_166_residues
MKNLWFYTCPIGKIGIVEKNGAICQVFFSTEAPPDGFTLQKSPVIERAEHQLTEYFAGDRKEFDLPLDLGGTEFQATVWQ
ALQTIPAGETRSYKDVAVMIGKPGASRAVGMANNRNPVAIIVPCHRVIGHDGSLTGYAGGLAIKQYLLDHEKMWQPERIH
AQYTLF
>Mature_166_residues
MKNLWFYTCPIGKIGIVEKNGAICQVFFSTEAPPDGFTLQKSPVIERAEHQLTEYFAGDRKEFDLPLDLGGTEFQATVWQ
ALQTIPAGETRSYKDVAVMIGKPGASRAVGMANNRNPVAIIVPCHRVIGHDGSLTGYAGGLAIKQYLLDHEKMWQPERIH
AQYTLF

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=75, Percent_Identity=46.6666666666667, Blast_Score=80, Evalue=7e-16,
Organism=Escherichia coli, GI1787596, Length=102, Percent_Identity=54.9019607843137, Blast_Score=120, Evalue=6e-29,
Organism=Escherichia coli, GI1788542, Length=153, Percent_Identity=39.8692810457516, Blast_Score=105, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI115533068, Length=82, Percent_Identity=56.0975609756098, Blast_Score=90, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI115533070, Length=82, Percent_Identity=56.0975609756098, Blast_Score=90, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6320001, Length=84, Percent_Identity=45.2380952380952, Blast_Score=84, Evalue=9e-18,
Organism=Drosophila melanogaster, GI17137554, Length=100, Percent_Identity=41, Blast_Score=72, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 18424; Mature: 18424

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNLWFYTCPIGKIGIVEKNGAICQVFFSTEAPPDGFTLQKSPVIERAEHQLTEYFAGDR
CCCCEEEECCCCCEEEEECCCCEEEEEEECCCCCCCEEECCCCHHHHHHHHHHHHHCCCC
KEFDLPLDLGGTEFQATVWQALQTIPAGETRSYKDVAVMIGKPGASRAVGMANNRNPVAI
CCCCCEECCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCEEECCCCCCCEEE
IVPCHRVIGHDGSLTGYAGGLAIKQYLLDHEKMWQPERIHAQYTLF
EEECHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEC
>Mature Secondary Structure
MKNLWFYTCPIGKIGIVEKNGAICQVFFSTEAPPDGFTLQKSPVIERAEHQLTEYFAGDR
CCCCEEEECCCCCEEEEECCCCEEEEEEECCCCCCCEEECCCCHHHHHHHHHHHHHCCCC
KEFDLPLDLGGTEFQATVWQALQTIPAGETRSYKDVAVMIGKPGASRAVGMANNRNPVAI
CCCCCEECCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCEEECCCCCCCEEE
IVPCHRVIGHDGSLTGYAGGLAIKQYLLDHEKMWQPERIHAQYTLF
EEECHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2505068; 2506524; 9384377 [H]