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The map label for this gene is spsK [H]

Identifier: 220904398

GI number: 220904398

Start: 1328395

End: 1329282

Strand: Reverse

Name: spsK [H]

Synonym: Ddes_1128

Alternate gene names: 220904398

Gene position: 1329282-1328395 (Counterclockwise)

Preceding gene: 220904401

Following gene: 220904397

Centisome position: 46.26

GC content: 58.56

Gene sequence:

>888_bases
ATGGCAAAAGCGCTGATTCTGGGAGGCGCAACCGGTCTGCTGGGGCAGGCCCTTGTTCGTGTGCTTGCGGCACGAGGCTG
GCAAGTGGCGACCCTGGGCAGGCAGGACGGCAATGTGATGGATTTTGACTTCTTACAGTCACGTCTGCTTGCGGCACACG
CGGACGTGGTTTTTAACGCTGTAGGCTTTACGGCTGTTGACGCTGCCGAAGATGACCCCGACGCGGCTTGTGAGGCAAAC
CGCACCCTGCCCGATGCGTTGGCGCACATTCTTAAAACTCTCGGCAACGGATACCTGGTCCACTACAGCACCGACTTTGT
TTTTCAGGGCCAAGGAGAAACTCCGCTGACCGAAGAAGACGAGCCGCATCCCTTGTCGGTATACGGCAGCACCAAGCTCG
AAGGCGAGCAGGCTATACTGCGCGAATTGCCCGAGCGTTCCTGCGTGCTGCGCACGGCCTGGCTTTTTGGCCCCGGCCGC
AAAAATTTTGTGGACACCATCGTGGCAGCCTGCGAAAAACGCGACACCATCAACGTGGTTCACGATCAGGTTGGCTCCCC
CACGTATAGTATGGATCTGGCGCAATGGAGCGCCGCCCTGGCTGAAAAACAGGCCACAGGACTCTGGCACGCCGTTAACA
GCGGCCAGGCCAGCTGGTGCGAACTGGCTTGCGAGTCCATCAACCTCGCGGCCGCTTCCTGCCGGGTTATGCCCATCGAT
TCGGCCCAATGGCCCCAAAAAGCCCAACGGCCGGAGTTTTCCGTGCTGGATAACAATAAACTGGGCGCCTTCCTGGGCAA
AAAGCCACGGCCATGGCCCCAGGCTCTGCGCGACTATATTTTTAGTGATTACCTGCCGTCTCACCGTAAGGAGAAAGACA
AGCATTGA

Upstream 100 bases:

>100_bases
AAGCGTTGTATTGACCAGCATGACTCACAACGATACAGTGTCTGCATTATGGGACGTACGCTCCCTCTGGGGAAGGCGTG
TACCGTGCCGGGAGGTTCAG

Downstream 100 bases:

>100_bases
CCTCTCGCCAGCAGACCATTCCTCTTCTGCCAGGCCCCAGCCGAGACGCCACGGCAGGTAACTGCACCTGCAGGACACGT
GTACGGCTCACCAACGGCCG

Product: dTDP-4-dehydrorhamnose reductase

Products: dTDP-4-dehydro-6-deoxy-L-mannose; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MAKALILGGATGLLGQALVRVLAARGWQVATLGRQDGNVMDFDFLQSRLLAAHADVVFNAVGFTAVDAAEDDPDAACEAN
RTLPDALAHILKTLGNGYLVHYSTDFVFQGQGETPLTEEDEPHPLSVYGSTKLEGEQAILRELPERSCVLRTAWLFGPGR
KNFVDTIVAACEKRDTINVVHDQVGSPTYSMDLAQWSAALAEKQATGLWHAVNSGQASWCELACESINLAAASCRVMPID
SAQWPQKAQRPEFSVLDNNKLGAFLGKKPRPWPQALRDYIFSDYLPSHRKEKDKH

Sequences:

>Translated_295_residues
MAKALILGGATGLLGQALVRVLAARGWQVATLGRQDGNVMDFDFLQSRLLAAHADVVFNAVGFTAVDAAEDDPDAACEAN
RTLPDALAHILKTLGNGYLVHYSTDFVFQGQGETPLTEEDEPHPLSVYGSTKLEGEQAILRELPERSCVLRTAWLFGPGR
KNFVDTIVAACEKRDTINVVHDQVGSPTYSMDLAQWSAALAEKQATGLWHAVNSGQASWCELACESINLAAASCRVMPID
SAQWPQKAQRPEFSVLDNNKLGAFLGKKPRPWPQALRDYIFSDYLPSHRKEKDKH
>Mature_294_residues
AKALILGGATGLLGQALVRVLAARGWQVATLGRQDGNVMDFDFLQSRLLAAHADVVFNAVGFTAVDAAEDDPDAACEANR
TLPDALAHILKTLGNGYLVHYSTDFVFQGQGETPLTEEDEPHPLSVYGSTKLEGEQAILRELPERSCVLRTAWLFGPGRK
NFVDTIVAACEKRDTINVVHDQVGSPTYSMDLAQWSAALAEKQATGLWHAVNSGQASWCELACESINLAAASCRVMPIDS
AQWPQKAQRPEFSVLDNNKLGAFLGKKPRPWPQALRDYIFSDYLPSHRKEKDKH

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI11034825, Length=302, Percent_Identity=28.8079470198675, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI33519455, Length=302, Percent_Identity=28.8079470198675, Blast_Score=96, Evalue=5e-20,
Organism=Escherichia coli, GI1788352, Length=271, Percent_Identity=32.4723247232472, Blast_Score=133, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: 1.1.1.133

Molecular weight: Translated: 32274; Mature: 32143

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKALILGGATGLLGQALVRVLAARGWQVATLGRQDGNVMDFDFLQSRLLAAHADVVFNA
CCCEEEECCCHHHHHHHHHHHHHHCCCEEEEECCCCCCEECHHHHHHHHHHHHHHHHHHH
VGFTAVDAAEDDPDAACEANRTLPDALAHILKTLGNGYLVHYSTDFVFQGQGETPLTEED
HCCEEECCCCCCCCHHHHCCCCCHHHHHHHHHHHCCCEEEEEECCEEEECCCCCCCCCCC
EPHPLSVYGSTKLEGEQAILRELPERSCVLRTAWLFGPGRKNFVDTIVAACEKRDTINVV
CCCCEEEECCCCCCHHHHHHHHCCCHHHHHHHHEEECCCCHHHHHHHHHHHHCCCCHHHH
HDQVGSPTYSMDLAQWSAALAEKQATGLWHAVNSGQASWCELACESINLAAASCRVMPID
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHCCEEHHCEEEEECC
SAQWPQKAQRPEFSVLDNNKLGAFLGKKPRPWPQALRDYIFSDYLPSHRKEKDKH
CCCCCHHHCCCCEEEECCCCCHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHCCCC
>Mature Secondary Structure 
AKALILGGATGLLGQALVRVLAARGWQVATLGRQDGNVMDFDFLQSRLLAAHADVVFNA
CCEEEECCCHHHHHHHHHHHHHHCCCEEEEECCCCCCEECHHHHHHHHHHHHHHHHHHH
VGFTAVDAAEDDPDAACEANRTLPDALAHILKTLGNGYLVHYSTDFVFQGQGETPLTEED
HCCEEECCCCCCCCHHHHCCCCCHHHHHHHHHHHCCCEEEEEECCEEEECCCCCCCCCCC
EPHPLSVYGSTKLEGEQAILRELPERSCVLRTAWLFGPGRKNFVDTIVAACEKRDTINVV
CCCCEEEECCCCCCHHHHHHHHCCCHHHHHHHHEEECCCCHHHHHHHHHHHHCCCCHHHH
HDQVGSPTYSMDLAQWSAALAEKQATGLWHAVNSGQASWCELACESINLAAASCRVMPID
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHCCEEHHCEEEEECC
SAQWPQKAQRPEFSVLDNNKLGAFLGKKPRPWPQALRDYIFSDYLPSHRKEKDKH
CCCCCHHHCCCCEEEECCCCCHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-6-deoxy-L-mannose; NADP+

Specific reaction: dTDP-6-deoxy-L-mannose + NADP+ = dTDP-4-dehydro-6-deoxy-L-mannose + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 10568751 [H]