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The map label for this gene is rnhB [H]

Identifier: 21910354

GI number: 21910354

Start: 870741

End: 871532

Strand: Direct

Name: rnhB [H]

Synonym: SpyM3_0818

Alternate gene names: 21910354

Gene position: 870741-871532 (Clockwise)

Preceding gene: 21910353

Following gene: 21910355

Centisome position: 45.82

GC content: 41.92

Gene sequence:

>792_bases
ATGCCGACTAGTATTAAAGCTATTAAAGAAAGCTTAGAGGCCGTTACTAGCCTCTTGGACCCCCTCTTTCAAGAATTGGC
AACCGACACTAGGTCAGGCGTCCAAAAAGCGCTAAAAAGTCGACAAAAGGCTATTCAGGCCGATTTAGCAGAGGAAGAAC
GATTAGAAGCCATGCTTTCTTATGAAAAAGCGCTTTATAAAGAAGGCTACCAAGCCATTGCAGGTATTGATGAGGTGGGA
CGTGGCCCCCTAGCAGGTCCCGTTGTCGCAGCATGTGTCATCTTACCTCAACATTGCAAAATTAAAGGTCTGAATGATTC
TAAAAAAATCCCTAAAGCTAAGCATGAGACCATTTATCAGGCAGTGAAAGAAAAGGCTTTGGCTATCGGTATCGGTATTA
TTGACAATCAGCTGATTGATGAGGTCAACATTTATGAAGCAACCAAACTAGCTATGCTAGAAGCCATTAAACAGTTGGAG
GGCCAACTCACACAACCAGATTATCTCTTGATTGATGCCATGACATTGGATATTGCTATTTCGCAGCAGTCTATTCTTAA
AGGCGATGCCAATTCCTTGTCTATTGCAGCAGCATCAATTGTAGCTAAGGTCACCAGAGATCAGATGATGGCTAACTATG
ATCGCATTTTTCCTGGTTATGGCTTTGCTAAAAATGCAGGCTATGGCACCAAAGAACATTTACAGGAATTAAAAGCTTAC
GGCATAACGCCTATCCATCGTAAAAGTTTTGAACCTGTTAAATCCATGTGCTGCGATTCAACTAATCCTTAA

Upstream 100 bases:

>100_bases
GGCTTTAAAGATGATTACGACCGTTTCTACAGTTTATTCGTTAAGGAAGTTCGTGATGGTAAATTAGGTCAATACACCTT
AGATCAGGTGGGAGATATGG

Downstream 100 bases:

>100_bases
AAAGTTCTGTTTTTAGCAGAATTTTTTTCTTTTTTACGAATAAATGAAGTAAGGAGGTAACTTAGTGAATCATTTTGAAC
TTTATAAGCTAAAGAAAGCT

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII [H]

Number of amino acids: Translated: 263; Mature: 262

Protein sequence:

>263_residues
MPTSIKAIKESLEAVTSLLDPLFQELATDTRSGVQKALKSRQKAIQADLAEEERLEAMLSYEKALYKEGYQAIAGIDEVG
RGPLAGPVVAACVILPQHCKIKGLNDSKKIPKAKHETIYQAVKEKALAIGIGIIDNQLIDEVNIYEATKLAMLEAIKQLE
GQLTQPDYLLIDAMTLDIAISQQSILKGDANSLSIAAASIVAKVTRDQMMANYDRIFPGYGFAKNAGYGTKEHLQELKAY
GITPIHRKSFEPVKSMCCDSTNP

Sequences:

>Translated_263_residues
MPTSIKAIKESLEAVTSLLDPLFQELATDTRSGVQKALKSRQKAIQADLAEEERLEAMLSYEKALYKEGYQAIAGIDEVG
RGPLAGPVVAACVILPQHCKIKGLNDSKKIPKAKHETIYQAVKEKALAIGIGIIDNQLIDEVNIYEATKLAMLEAIKQLE
GQLTQPDYLLIDAMTLDIAISQQSILKGDANSLSIAAASIVAKVTRDQMMANYDRIFPGYGFAKNAGYGTKEHLQELKAY
GITPIHRKSFEPVKSMCCDSTNP
>Mature_262_residues
PTSIKAIKESLEAVTSLLDPLFQELATDTRSGVQKALKSRQKAIQADLAEEERLEAMLSYEKALYKEGYQAIAGIDEVGR
GPLAGPVVAACVILPQHCKIKGLNDSKKIPKAKHETIYQAVKEKALAIGIGIIDNQLIDEVNIYEATKLAMLEAIKQLEG
QLTQPDYLLIDAMTLDIAISQQSILKGDANSLSIAAASIVAKVTRDQMMANYDRIFPGYGFAKNAGYGTKEHLQELKAYG
ITPIHRKSFEPVKSMCCDSTNP

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids [H]

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family [H]

Homologues:

Organism=Escherichia coli, GI1786380, Length=185, Percent_Identity=47.5675675675676, Blast_Score=160, Evalue=5e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01351 RNase_HII [H]

EC number: =3.1.26.4 [H]

Molecular weight: Translated: 28710; Mature: 28578

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTSIKAIKESLEAVTSLLDPLFQELATDTRSGVQKALKSRQKAIQADLAEEERLEAMLS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YEKALYKEGYQAIAGIDEVGRGPLAGPVVAACVILPQHCKIKGLNDSKKIPKAKHETIYQ
HHHHHHHHHHHHHHCHHHHCCCCCCHHHHHHHHHHHCCCEECCCCCHHCCCCHHHHHHHH
AVKEKALAIGIGIIDNQLIDEVNIYEATKLAMLEAIKQLEGQLTQPDYLLIDAMTLDIAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHH
SQQSILKGDANSLSIAAASIVAKVTRDQMMANYDRIFPGYGFAKNAGYGTKEHLQELKAY
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
GITPIHRKSFEPVKSMCCDSTNP
CCCCHHHCCHHHHHHHHCCCCCC
>Mature Secondary Structure 
PTSIKAIKESLEAVTSLLDPLFQELATDTRSGVQKALKSRQKAIQADLAEEERLEAMLS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YEKALYKEGYQAIAGIDEVGRGPLAGPVVAACVILPQHCKIKGLNDSKKIPKAKHETIYQ
HHHHHHHHHHHHHHCHHHHCCCCCCHHHHHHHHHHHCCCEECCCCCHHCCCCHHHHHHHH
AVKEKALAIGIGIIDNQLIDEVNIYEATKLAMLEAIKQLEGQLTQPDYLLIDAMTLDIAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHH
SQQSILKGDANSLSIAAASIVAKVTRDQMMANYDRIFPGYGFAKNAGYGTKEHLQELKAY
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
GITPIHRKSFEPVKSMCCDSTNP
CCCCHHHCCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11296296 [H]