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The map label for this gene is acoA [H]

Identifier: 21910197

GI number: 21910197

Start: 723233

End: 724201

Strand: Direct

Name: acoA [H]

Synonym: SpyM3_0661

Alternate gene names: 21910197

Gene position: 723233-724201 (Clockwise)

Preceding gene: 21910196

Following gene: 21910198

Centisome position: 38.05

GC content: 41.59

Gene sequence:

>969_bases
ATGGTAACAGTTTCTAAAGAACAACATTTAGATATGTTCCTTAAAATGGAACGTATTCGTGAATTTGATTCACGTATTAA
TAAACTCGTCCGTCGTGGATTTGTACAAGGGATGACTCACTTCTCAGTTGGTGAAGAAGCAGCCAATGTTGGAGCGGTGG
CTCACTTGTCTTACGATGACATTATTTTTTCAAATCACCGTGGACATGGACAATCAATCGCTAAAGACATGGACCTTAAT
AAAATGATGGCTGAGCTTGCTGGTAAAGCAACTGGTGTTTCTAAAGGGCGTGGTGGCTCAATGCACTTGGCTGATTTTGA
AAAAGGAAACTATGGTACCAATGGTATCGTTGGTGGTGGTTACGCTCTTGCAGTTGGTGCTGCCCTCACCCAACAATACA
AAGGAACTAACAATATAGCTGTTGCTTTCTCAGGTGATGGCGCTACTAACGAAGGATCATTCCACGAGTCTGTTAATATG
GCTGCAACATGGAAACTTCCTGTTATTTTCTTCATCATCAACAACCGTTATGGAATTTCAATGAGCATCAATAATGCAAC
AAATACACCACACTTATACACACGTGCAGAAGCTTACGGAATTCCTGGTTTCTACTGTGAAGATGGCAATGACGTGATGG
CTGTCTATGAAACAATGGGCAAAGCCGTTGAACATGTTCGTGGTGGAAATGGCCCAGCTATTGTTGAGGTAGAATCATAC
CGTTGGTTTGGTCACTCGACTGCAGATGCTGGTAAATACCGTACTAAAGAAGAAGTGGATGAGTGGAAAGAAAAAGATCC
AATGATCAAATATCGTACTTATCTGACAAGCGAAGGCATTGCCACTGATGACGAATTGGATGCTATTCAAGCACAAGTCA
AAAAAGAAGTTGACGATGCTTATGAATTTGCACAAAACAGTCCAGATCCAGAACTTTCAGTAGCCTTCGAAGACGTTTGG
GTTGACTAA

Upstream 100 bases:

>100_bases
AAAAAACCAAATAAGGTAAGGTAGAAAGCGATTTAATTATTTGCAAATTGTGAAAAAAAGTTTTATCATAAAAAGGTAAT
ATGATAATATGGAGGCCGAA

Downstream 100 bases:

>100_bases
GATCACCTTGTCAGTAAGAAGCAATCGTATTTGAATAATAAATGGAGAAATAAAAATGATGTCAGAAACAAAATTAATGG
CTTTGCGTGAAGCAGTCAAC

Product: putative acetoin dehydrogenase (TPP-dependent) alpha chain

Products: [dihydrolipoyllysine-residue acetyltransferase] ; $S-acetyldihydrolipoyllysine; CO2

Alternate protein names: Acetoin:DCPIP oxidoreductase-alpha; Ao:DCPIP OR; TPP-dependent acetoin dehydrogenase E1 subunit alpha [H]

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MVTVSKEQHLDMFLKMERIREFDSRINKLVRRGFVQGMTHFSVGEEAANVGAVAHLSYDDIIFSNHRGHGQSIAKDMDLN
KMMAELAGKATGVSKGRGGSMHLADFEKGNYGTNGIVGGGYALAVGAALTQQYKGTNNIAVAFSGDGATNEGSFHESVNM
AATWKLPVIFFIINNRYGISMSINNATNTPHLYTRAEAYGIPGFYCEDGNDVMAVYETMGKAVEHVRGGNGPAIVEVESY
RWFGHSTADAGKYRTKEEVDEWKEKDPMIKYRTYLTSEGIATDDELDAIQAQVKKEVDDAYEFAQNSPDPELSVAFEDVW
VD

Sequences:

>Translated_322_residues
MVTVSKEQHLDMFLKMERIREFDSRINKLVRRGFVQGMTHFSVGEEAANVGAVAHLSYDDIIFSNHRGHGQSIAKDMDLN
KMMAELAGKATGVSKGRGGSMHLADFEKGNYGTNGIVGGGYALAVGAALTQQYKGTNNIAVAFSGDGATNEGSFHESVNM
AATWKLPVIFFIINNRYGISMSINNATNTPHLYTRAEAYGIPGFYCEDGNDVMAVYETMGKAVEHVRGGNGPAIVEVESY
RWFGHSTADAGKYRTKEEVDEWKEKDPMIKYRTYLTSEGIATDDELDAIQAQVKKEVDDAYEFAQNSPDPELSVAFEDVW
VD
>Mature_322_residues
MVTVSKEQHLDMFLKMERIREFDSRINKLVRRGFVQGMTHFSVGEEAANVGAVAHLSYDDIIFSNHRGHGQSIAKDMDLN
KMMAELAGKATGVSKGRGGSMHLADFEKGNYGTNGIVGGGYALAVGAALTQQYKGTNNIAVAFSGDGATNEGSFHESVNM
AATWKLPVIFFIINNRYGISMSINNATNTPHLYTRAEAYGIPGFYCEDGNDVMAVYETMGKAVEHVRGGNGPAIVEVESY
RWFGHSTADAGKYRTKEEVDEWKEKDPMIKYRTYLTSEGIATDDELDAIQAQVKKEVDDAYEFAQNSPDPELSVAFEDVW
VD

Specific function: Catalyzes the 2,6-dichlorophenolindophenol-dependent cleavage of acetoin into acetate and acetaldehyde. The alpha subunit is probably the catalytic subunit of the enzyme [H]

COG id: COG1071

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4885543, Length=312, Percent_Identity=38.1410256410256, Blast_Score=233, Evalue=1e-61,
Organism=Homo sapiens, GI4505685, Length=312, Percent_Identity=36.2179487179487, Blast_Score=209, Evalue=2e-54,
Organism=Homo sapiens, GI291084742, Length=312, Percent_Identity=36.2179487179487, Blast_Score=209, Evalue=3e-54,
Organism=Homo sapiens, GI291084744, Length=319, Percent_Identity=35.423197492163, Blast_Score=202, Evalue=3e-52,
Organism=Homo sapiens, GI291084757, Length=312, Percent_Identity=32.6923076923077, Blast_Score=171, Evalue=8e-43,
Organism=Homo sapiens, GI11386135, Length=320, Percent_Identity=31.25, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI258645172, Length=320, Percent_Identity=31.25, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI17536047, Length=307, Percent_Identity=36.1563517915309, Blast_Score=208, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI32564172, Length=307, Percent_Identity=36.1563517915309, Blast_Score=208, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI86563355, Length=315, Percent_Identity=32.3809523809524, Blast_Score=144, Evalue=7e-35,
Organism=Caenorhabditis elegans, GI86563357, Length=315, Percent_Identity=32.3809523809524, Blast_Score=144, Evalue=7e-35,
Organism=Saccharomyces cerevisiae, GI6321026, Length=322, Percent_Identity=37.5776397515528, Blast_Score=215, Evalue=8e-57,
Organism=Drosophila melanogaster, GI24639740, Length=323, Percent_Identity=38.0804953560372, Blast_Score=212, Evalue=2e-55,
Organism=Drosophila melanogaster, GI24639744, Length=323, Percent_Identity=38.0804953560372, Blast_Score=212, Evalue=3e-55,
Organism=Drosophila melanogaster, GI28571106, Length=323, Percent_Identity=38.0804953560372, Blast_Score=212, Evalue=3e-55,
Organism=Drosophila melanogaster, GI24639746, Length=309, Percent_Identity=38.5113268608414, Blast_Score=203, Evalue=1e-52,
Organism=Drosophila melanogaster, GI24639748, Length=321, Percent_Identity=35.202492211838, Blast_Score=201, Evalue=7e-52,
Organism=Drosophila melanogaster, GI21355903, Length=316, Percent_Identity=27.5316455696203, Blast_Score=112, Evalue=4e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001017 [H]

Pfam domain/function: PF00676 E1_dh [H]

EC number: 1.2.4.1

Molecular weight: Translated: 35459; Mature: 35459

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTVSKEQHLDMFLKMERIREFDSRINKLVRRGFVQGMTHFSVGEEAANVGAVAHLSYDD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCHHHHCCCEEEEECCCC
IIFSNHRGHGQSIAKDMDLNKMMAELAGKATGVSKGRGGSMHLADFEKGNYGTNGIVGGG
EEEECCCCCCHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCEECCH
YALAVGAALTQQYKGTNNIAVAFSGDGATNEGSFHESVNMAATWKLPVIFFIINNRYGIS
HHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHCCCEEEEECCEEEEEEEECCEEEE
MSINNATNTPHLYTRAEAYGIPGFYCEDGNDVMAVYETMGKAVEHVRGGNGPAIVEVESY
EEECCCCCCCEEEEEHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECE
RWFGHSTADAGKYRTKEEVDEWKEKDPMIKYRTYLTSEGIATDDELDAIQAQVKKEVDDA
EEECCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHH
YEFAQNSPDPELSVAFEDVWVD
HHHHCCCCCCCEEEEEEEEECC
>Mature Secondary Structure
MVTVSKEQHLDMFLKMERIREFDSRINKLVRRGFVQGMTHFSVGEEAANVGAVAHLSYDD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCHHHHCCCEEEEECCCC
IIFSNHRGHGQSIAKDMDLNKMMAELAGKATGVSKGRGGSMHLADFEKGNYGTNGIVGGG
EEEECCCCCCHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCEECCH
YALAVGAALTQQYKGTNNIAVAFSGDGATNEGSFHESVNMAATWKLPVIFFIINNRYGIS
HHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHCCCEEEEECCEEEEEEEECCEEEE
MSINNATNTPHLYTRAEAYGIPGFYCEDGNDVMAVYETMGKAVEHVRGGNGPAIVEVESY
EEECCCCCCCEEEEEHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECE
RWFGHSTADAGKYRTKEEVDEWKEKDPMIKYRTYLTSEGIATDDELDAIQAQVKKEVDDA
EEECCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHH
YEFAQNSPDPELSVAFEDVWVD
HHHHCCCCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: pyruvate; [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine

Specific reaction: pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S- $acetyldihydrolipoyllysine + CO2

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 10368162; 9384377 [H]