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The map label for this gene is murN [H]

Identifier: 21909970

GI number: 21909970

Start: 476531

End: 477766

Strand: Reverse

Name: murN [H]

Synonym: SpyM3_0434

Alternate gene names: 21909970

Gene position: 477766-476531 (Counterclockwise)

Preceding gene: 21909971

Following gene: 21909965

Centisome position: 25.14

GC content: 36.0

Gene sequence:

>1236_bases
ATGGCACTAATTGAAATTTCACAAGAACAATTTGACCATTACTGCCACAGTCTCGTCCGTCATTCCTTTATCCAAACCTC
AGAAATGGCCAGCTTGATGGCTAAAAGAGGGGCAAAACCACAATTTCTTGGACTTGAAAAAGATGGTGAACTAAAGGTTG
CTGCTATGGTATTTAGCCAAAAAGTGGCTGGAGGTTGGCGTATGGAACTCAATGCAGGCCCTAATACCAATCATCCCGAA
GAATTAGAACATTTTTACACTCAACTTAAAGACTATGCCAAACAAAAAGACGTTATCGAACTCATTTTAAAACCTTACGA
CAATTATCAGTCATTTGATACTGATGGCATCCCTATTTCTCATCCAAACACTGACCTTATTAGCCTTTTAACGGCGTTAG
GATATAAACATGACGGTTTAAAAACAGGCTATCCTGAGGGTGAACCTGTTTGGCATTACGTTAAAAAGTTAGAAGGAATT
GATTCCTCACGTTTAACCCATTCCTTCAGCAAAAAAGGAAAAGCTTTAATAAAAAAAGCTAATACTTTTGGTATTAAATT
ACGTCAACTGAAACGAGATGAGTTACACCATTTCAAAGAAATCACTGAAGCAACCTCTGATCGTAGAGATTACCTTGACA
AATCATTATCTTACTATCAGGACTTTTATGATAGTTTTGGAGACTCTTGTGAATTTATGGTTGCAACTCTTAATTTTGAA
GATTACCTTAATAACCTTAAACAAAGACAACTACAACTAGCAACCAGCATCAACAAAGTTAAAGGAGACCTTGGCAAAAA
TCCTCATTCTGAAAAAAAGCAAAATAGATTAAAAGAACTATCCAGCCAATTCGAAACCTTTCAGGTAAGAATTTCTGAAG
CTCTCCATTTTCTTGAAGAGTACGGAACTAAAGATGTGTTCCTTGCGGGAAGTCTTTTTATCTATACCAAACAAGAGGCC
GTCTACCTCTTTTCAGGATCTTACCCCAAATTTAATAAATTTTATAGCCCAGCTCTATTACAAGAGCATGCTATGCTCAA
GGCAATTCACAAGGGCATCAAACAATATAATTTTCTTGGTATTACTGGAAAATTTGATGGTTCAGACGGTGTCTTGCGCT
TTAAACAGAATTTCAATGGCTTTATTTTGCAAAAACCAGGAACATTTAGATGTTATCCATTTCCAATCAAATATCATTTC
ATTAGATTGGCAAAAAAGCTATTAAACCGCTATTAG

Upstream 100 bases:

>100_bases
ATCGAAGAATTTACAGGTGAATTCAACATTCCTGTCGGCCTTGTTTCAAGCTTAGCCATCTTGACCTATAACTTAAGAAA
AAAATTAAGGAGCACAACTA

Downstream 100 bases:

>100_bases
GCTAATGCATTTGCCTGTAGAATCACCTTTATTCTCTCATAGTAAGAGTTAAGTCATCTAAAAAAGAAAAGGATTTAACC
TTTTCTTTTTTAGATGACTT

Product: putative peptidoglycan branched peptide synthesis protein, alanine adding enzyme

Products: NA

Alternate protein names: Factor essential for expression of methicillin resistance B [H]

Number of amino acids: Translated: 411; Mature: 410

Protein sequence:

>411_residues
MALIEISQEQFDHYCHSLVRHSFIQTSEMASLMAKRGAKPQFLGLEKDGELKVAAMVFSQKVAGGWRMELNAGPNTNHPE
ELEHFYTQLKDYAKQKDVIELILKPYDNYQSFDTDGIPISHPNTDLISLLTALGYKHDGLKTGYPEGEPVWHYVKKLEGI
DSSRLTHSFSKKGKALIKKANTFGIKLRQLKRDELHHFKEITEATSDRRDYLDKSLSYYQDFYDSFGDSCEFMVATLNFE
DYLNNLKQRQLQLATSINKVKGDLGKNPHSEKKQNRLKELSSQFETFQVRISEALHFLEEYGTKDVFLAGSLFIYTKQEA
VYLFSGSYPKFNKFYSPALLQEHAMLKAIHKGIKQYNFLGITGKFDGSDGVLRFKQNFNGFILQKPGTFRCYPFPIKYHF
IRLAKKLLNRY

Sequences:

>Translated_411_residues
MALIEISQEQFDHYCHSLVRHSFIQTSEMASLMAKRGAKPQFLGLEKDGELKVAAMVFSQKVAGGWRMELNAGPNTNHPE
ELEHFYTQLKDYAKQKDVIELILKPYDNYQSFDTDGIPISHPNTDLISLLTALGYKHDGLKTGYPEGEPVWHYVKKLEGI
DSSRLTHSFSKKGKALIKKANTFGIKLRQLKRDELHHFKEITEATSDRRDYLDKSLSYYQDFYDSFGDSCEFMVATLNFE
DYLNNLKQRQLQLATSINKVKGDLGKNPHSEKKQNRLKELSSQFETFQVRISEALHFLEEYGTKDVFLAGSLFIYTKQEA
VYLFSGSYPKFNKFYSPALLQEHAMLKAIHKGIKQYNFLGITGKFDGSDGVLRFKQNFNGFILQKPGTFRCYPFPIKYHF
IRLAKKLLNRY
>Mature_410_residues
ALIEISQEQFDHYCHSLVRHSFIQTSEMASLMAKRGAKPQFLGLEKDGELKVAAMVFSQKVAGGWRMELNAGPNTNHPEE
LEHFYTQLKDYAKQKDVIELILKPYDNYQSFDTDGIPISHPNTDLISLLTALGYKHDGLKTGYPEGEPVWHYVKKLEGID
SSRLTHSFSKKGKALIKKANTFGIKLRQLKRDELHHFKEITEATSDRRDYLDKSLSYYQDFYDSFGDSCEFMVATLNFED
YLNNLKQRQLQLATSINKVKGDLGKNPHSEKKQNRLKELSSQFETFQVRISEALHFLEEYGTKDVFLAGSLFIYTKQEAV
YLFSGSYPKFNKFYSPALLQEHAMLKAIHKGIKQYNFLGITGKFDGSDGVLRFKQNFNGFILQKPGTFRCYPFPIKYHFI
RLAKKLLNRY

Specific function: Catalyzes the formation of the pentaglycine interpeptide bridge, which is characteristic of the S.aureus peptidoglycan. Adds glycines 4 and 5 of the pentaglycine bridge, using glycyl- tRNA(Gly) as donor. Involved in resistance to methicillin [H]

COG id: COG2348

COG function: function code V; Uncharacterized protein involved in methicillin resistance

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the femABX family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016181
- InterPro:   IPR003447 [H]

Pfam domain/function: PF02388 FemAB [H]

EC number: NA

Molecular weight: Translated: 47509; Mature: 47378

Theoretical pI: Translated: 9.42; Mature: 9.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALIEISQEQFDHYCHSLVRHSFIQTSEMASLMAKRGAKPQFLGLEKDGELKVAAMVFSQ
CCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCEEHHHHHHHH
KVAGGWRMELNAGPNTNHPEELEHFYTQLKDYAKQKDVIELILKPYDNYQSFDTDGIPIS
HHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
HPNTDLISLLTALGYKHDGLKTGYPEGEPVWHYVKKLEGIDSSRLTHSFSKKGKALIKKA
CCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHC
NTFGIKLRQLKRDELHHFKEITEATSDRRDYLDKSLSYYQDFYDSFGDSCEFMVATLNFE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECHH
DYLNNLKQRQLQLATSINKVKGDLGKNPHSEKKQNRLKELSSQFETFQVRISEALHFLEE
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YGTKDVFLAGSLFIYTKQEAVYLFSGSYPKFNKFYSPALLQEHAMLKAIHKGIKQYNFLG
HCCCCEEEECEEEEEEECCEEEEEECCCCCHHHHCCHHHHHHHHHHHHHHHHHHHCCEEE
ITGKFDGSDGVLRFKQNFNGFILQKPGTFRCYPFPIKYHFIRLAKKLLNRY
EEEEECCCCCEEEEECCCCEEEEECCCCEEEEECHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ALIEISQEQFDHYCHSLVRHSFIQTSEMASLMAKRGAKPQFLGLEKDGELKVAAMVFSQ
CEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCEEHHHHHHHH
KVAGGWRMELNAGPNTNHPEELEHFYTQLKDYAKQKDVIELILKPYDNYQSFDTDGIPIS
HHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
HPNTDLISLLTALGYKHDGLKTGYPEGEPVWHYVKKLEGIDSSRLTHSFSKKGKALIKKA
CCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHC
NTFGIKLRQLKRDELHHFKEITEATSDRRDYLDKSLSYYQDFYDSFGDSCEFMVATLNFE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECHH
DYLNNLKQRQLQLATSINKVKGDLGKNPHSEKKQNRLKELSSQFETFQVRISEALHFLEE
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YGTKDVFLAGSLFIYTKQEAVYLFSGSYPKFNKFYSPALLQEHAMLKAIHKGIKQYNFLG
HCCCCEEEECEEEEEEECCEEEEEECCCCCHHHHCCHHHHHHHHHHHHHHHHHHHCCEEE
ITGKFDGSDGVLRFKQNFNGFILQKPGTFRCYPFPIKYHFIRLAKKLLNRY
EEEEECCCCCEEEEECCCCEEEEECCCCEEEEECHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA