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The map label for this gene is rfaH [H]

Identifier: 218930774

GI number: 218930774

Start: 4234847

End: 4235335

Strand: Direct

Name: rfaH [H]

Synonym: YPO3770

Alternate gene names: 218930774

Gene position: 4234847-4235335 (Clockwise)

Preceding gene: 218930771

Following gene: 218930789

Centisome position: 91.0

GC content: 44.17

Gene sequence:

>489_bases
ATGAAATCTTGGTATTTATTGTATTGTAAACGCGGTCAGATTTTACGTGCCAAAGAGCATTTGGAACGGCAAACTGTAAA
CTGTTGGACACCAATAGTGGCTATCGAAAAGATAGTTCGAGGGAAACGTATTGAGGTAATAGAGGCTCTATTCCCCAATT
ATTTATTCGCTGAGTTTGACCCGGAAAATATCCATACCACAACCGTCAGCGCCACCCGGGGGGGCAGCCATTTTGTTCGT
TTTGGTACACAGCCCGCGGTGATCCCAGCAACCGTCATTGCTGATATGCAAGCCCATGCTGTGGATAAGATAATTGCCCC
TGAAGTACCGAAACCTGGTGATATCGTAAAAATTATTGATGGCGTTTTTGCCGGATTACAGGCTATTTATACCGAACCAG
ATGGTGAAGCCCGCTCGATGTTGTTACTGAATATGCTCAATAGCCAAATTAAGCATAGCCTGGACAATCGTCAGTTCGAA
AAGCAATAA

Upstream 100 bases:

>100_bases
CAAAATAATCTGTATATTGGAAGTGATCCATTTTTCCTCACTATATCTCGCTCACTGCTTTTGTTATGCTGCTAAATAGT
ACCCAAAGGTATGTGAAACT

Downstream 100 bases:

>100_bases
CACGCTGACTTACAATAATCTGGATGAGTATTACCTCATCATTATAGAATTCATTCAGATAGGCGCAGTTAGTGCCTATC
TGAATGCTGTGACTCTTAGT

Product: transcriptional activator RfaH

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 162; Mature: 162

Protein sequence:

>162_residues
MKSWYLLYCKRGQILRAKEHLERQTVNCWTPIVAIEKIVRGKRIEVIEALFPNYLFAEFDPENIHTTTVSATRGGSHFVR
FGTQPAVIPATVIADMQAHAVDKIIAPEVPKPGDIVKIIDGVFAGLQAIYTEPDGEARSMLLLNMLNSQIKHSLDNRQFE
KQ

Sequences:

>Translated_162_residues
MKSWYLLYCKRGQILRAKEHLERQTVNCWTPIVAIEKIVRGKRIEVIEALFPNYLFAEFDPENIHTTTVSATRGGSHFVR
FGTQPAVIPATVIADMQAHAVDKIIAPEVPKPGDIVKIIDGVFAGLQAIYTEPDGEARSMLLLNMLNSQIKHSLDNRQFE
KQ
>Mature_162_residues
MKSWYLLYCKRGQILRAKEHLERQTVNCWTPIVAIEKIVRGKRIEVIEALFPNYLFAEFDPENIHTTTVSATRGGSHFVR
FGTQPAVIPATVIADMQAHAVDKIIAPEVPKPGDIVKIIDGVFAGLQAIYTEPDGEARSMLLLNMLNSQIKHSLDNRQFE
KQ

Specific function: Transcriptional activator of hemolysin synthesis and secretion, also required for expression of sex factor and lipopolysaccharide biosynthesis genes. Transcriptional activator of rfaD/htrM and htrL [H]

COG id: COG0250

COG function: function code K; Transcription antiterminator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790276, Length=161, Percent_Identity=63.9751552795031, Blast_Score=229, Evalue=6e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005824
- InterPro:   IPR010215
- InterPro:   IPR006645
- InterPro:   IPR014722 [H]

Pfam domain/function: PF02357 NusG [H]

EC number: NA

Molecular weight: Translated: 18286; Mature: 18286

Theoretical pI: Translated: 8.24; Mature: 8.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSWYLLYCKRGQILRAKEHLERQTVNCWTPIVAIEKIVRGKRIEVIEALFPNYLFAEFD
CCCEEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEECC
PENIHTTTVSATRGGSHFVRFGTQPAVIPATVIADMQAHAVDKIIAPEVPKPGDIVKIID
CCCCEEEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
GVFAGLQAIYTEPDGEARSMLLLNMLNSQIKHSLDNRQFEKQ
HHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKSWYLLYCKRGQILRAKEHLERQTVNCWTPIVAIEKIVRGKRIEVIEALFPNYLFAEFD
CCCEEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEECC
PENIHTTTVSATRGGSHFVRFGTQPAVIPATVIADMQAHAVDKIIAPEVPKPGDIVKIID
CCCCEEEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
GVFAGLQAIYTEPDGEARSMLLLNMLNSQIKHSLDNRQFEKQ
HHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]