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The map label for this gene is iscS [H]

Identifier: 218929955

GI number: 218929955

Start: 3238947

End: 3240176

Strand: Reverse

Name: iscS [H]

Synonym: YPO2896

Alternate gene names: 218929955

Gene position: 3240176-3238947 (Counterclockwise)

Preceding gene: 218929956

Following gene: 218929954

Centisome position: 69.63

GC content: 48.86

Gene sequence:

>1230_bases
ATGACCGAATCAAAAGTAAAGACACCGATCTATCTGGATTATGCAGCAACGACGCCAGTAGATCCGCGTGTCGCTGAAAA
AATGATGCAGTATCTCACTCTGGACGGTATCTTCGGTAACCCAGCCTCACGTTCGCATAAGTTTGGTTGGCAGGCAGAAG
AAGCTGTTGATATTGCACGTAATGACGTCGCCGCATTGGTGGGTGCAGACCCTCGTGAGATCGTCTTCACCTCCGGTGCG
ACCGAGTCTGATAACCTCGCCATTAAAGGTGCTGCTAATTTCTATCAGAAGAAAGGCAAGCACATCATCACTTGTAAAAC
CGAACATAAAGCAGTGTTGGATAGCTGTCGCCAATTGGAGCGTGAAGGCTTCGAAGTGACTTATCTGGCACCGCAGCCTA
ACGGTATTATCGATCTGAAACAGCTTGAAGCGACAATGCGTGAAGACACCATTTTGGTTTCTATCATGCATGTGAATAAT
GAGATCGGCGTGGTACAGGATATCGCTGAAATCGGCGAAATGTGCCGTAGCCGCGGGATCATTTTCCACGTTGATGCTAC
ACAAAGCGTCGGTAAATTACCTATTGATCTGAGCAAACTGAAAGTCGATTTGATGTCCTTCTCTGCCCATAAAGTTTATG
GCCCGATGGGCATTGGCGCACTGTTTGTTCGCCGTAAACCCCGTATTCGTATTGAAGCACAGCAGCACGGCGGTGGTCAT
GAGCGCGGTATGCGTTCAGGCACCTTGCCTGTGCACCAAATCGTTGGGATGGGTGAAGCTTACCGCATTGCCAAAGAAGA
GATGGAAAGCGAAGCTGCACGTCTGCGTTCACTGCGTTTGCGTTTGTGGAACGGCATCAAAGATATCGAAGAAGTTTACC
TGAACGGCTCATTAGAGAATGGCGCTCCTGGCATTCTGAACGTCAGCTTCAACTATGTTGAAGGTGAGTCGTTGATCATG
GCACTGAAAGATTTGGCTGTCTCATCAGGTTCAGCCTGTACCTCTGCGAGTTTGGAGCCTTCTTACGTGTTGCGTGCGTT
GGGGATGAACGACGAACTGGCTCACAGTTCAATTCGTTTCTCTCTGGGGCGTTTCACCACAGAAGAAGAGATCGACTACG
CCATTGCGCTGGTACATAAATCCATTGGTCGTCTGCGTGATTTATCCCCATTGTGGGATATGTTCAAGCAGGGCGTGGAT
ATCAGCAGCATTGAATGGTCCCATCATTAA

Upstream 100 bases:

>100_bases
CTAATGGCCGACCGCAAGAGACGATTAACGTCAATCTGCGCGCATAAGCAGAATTTGCGGAATTTTACGTTTTGGAAGTG
ATGTACGGAGCATAAGAGCA

Downstream 100 bases:

>100_bases
TTCCACACTTTAAGACTCAGGAGAAATCACATGGCTTACAGCGAAAAAGTAATCGATCATTACGAAAATCCACGCAACGT
GGGGTCGTTCGACAGTCAAG

Product: cysteine desulfurase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 409; Mature: 408

Protein sequence:

>409_residues
MTESKVKTPIYLDYAATTPVDPRVAEKMMQYLTLDGIFGNPASRSHKFGWQAEEAVDIARNDVAALVGADPREIVFTSGA
TESDNLAIKGAANFYQKKGKHIITCKTEHKAVLDSCRQLEREGFEVTYLAPQPNGIIDLKQLEATMREDTILVSIMHVNN
EIGVVQDIAEIGEMCRSRGIIFHVDATQSVGKLPIDLSKLKVDLMSFSAHKVYGPMGIGALFVRRKPRIRIEAQQHGGGH
ERGMRSGTLPVHQIVGMGEAYRIAKEEMESEAARLRSLRLRLWNGIKDIEEVYLNGSLENGAPGILNVSFNYVEGESLIM
ALKDLAVSSGSACTSASLEPSYVLRALGMNDELAHSSIRFSLGRFTTEEEIDYAIALVHKSIGRLRDLSPLWDMFKQGVD
ISSIEWSHH

Sequences:

>Translated_409_residues
MTESKVKTPIYLDYAATTPVDPRVAEKMMQYLTLDGIFGNPASRSHKFGWQAEEAVDIARNDVAALVGADPREIVFTSGA
TESDNLAIKGAANFYQKKGKHIITCKTEHKAVLDSCRQLEREGFEVTYLAPQPNGIIDLKQLEATMREDTILVSIMHVNN
EIGVVQDIAEIGEMCRSRGIIFHVDATQSVGKLPIDLSKLKVDLMSFSAHKVYGPMGIGALFVRRKPRIRIEAQQHGGGH
ERGMRSGTLPVHQIVGMGEAYRIAKEEMESEAARLRSLRLRLWNGIKDIEEVYLNGSLENGAPGILNVSFNYVEGESLIM
ALKDLAVSSGSACTSASLEPSYVLRALGMNDELAHSSIRFSLGRFTTEEEIDYAIALVHKSIGRLRDLSPLWDMFKQGVD
ISSIEWSHH
>Mature_408_residues
TESKVKTPIYLDYAATTPVDPRVAEKMMQYLTLDGIFGNPASRSHKFGWQAEEAVDIARNDVAALVGADPREIVFTSGAT
ESDNLAIKGAANFYQKKGKHIITCKTEHKAVLDSCRQLEREGFEVTYLAPQPNGIIDLKQLEATMREDTILVSIMHVNNE
IGVVQDIAEIGEMCRSRGIIFHVDATQSVGKLPIDLSKLKVDLMSFSAHKVYGPMGIGALFVRRKPRIRIEAQQHGGGHE
RGMRSGTLPVHQIVGMGEAYRIAKEEMESEAARLRSLRLRLWNGIKDIEEVYLNGSLENGAPGILNVSFNYVEGESLIMA
LKDLAVSSGSACTSASLEPSYVLRALGMNDELAHSSIRFSLGRFTTEEEIDYAIALVHKSIGRLRDLSPLWDMFKQGVDI
SSIEWSHH

Specific function: Catalyzes the removal of elemental sulfur and selenium atoms from cysteine and selenocysteine to produce alanine. Functions as a sulfur delivery protein for NAD, biotin and Fe-S cluster synthesis. Transfers sulfur on 'Cys-456' of ThiI in a transpersulfida

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=405, Percent_Identity=59.0123456790123, Blast_Score=520, Evalue=1e-148,
Organism=Homo sapiens, GI156713448, Length=416, Percent_Identity=29.5673076923077, Blast_Score=174, Evalue=1e-43,
Organism=Escherichia coli, GI48994898, Length=404, Percent_Identity=88.8613861386139, Blast_Score=757, Evalue=0.0,
Organism=Escherichia coli, GI1787970, Length=181, Percent_Identity=33.1491712707182, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1789175, Length=215, Percent_Identity=29.7674418604651, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI25143064, Length=403, Percent_Identity=59.3052109181141, Blast_Score=504, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI17533177, Length=324, Percent_Identity=32.4074074074074, Blast_Score=141, Evalue=8e-34,
Organism=Saccharomyces cerevisiae, GI6319831, Length=401, Percent_Identity=55.860349127182, Blast_Score=483, Evalue=1e-137,
Organism=Drosophila melanogaster, GI20129463, Length=402, Percent_Identity=57.7114427860697, Blast_Score=493, Evalue=1e-140,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 45258; Mature: 45127

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTESKVKTPIYLDYAATTPVDPRVAEKMMQYLTLDGIFGNPASRSHKFGWQAEEAVDIAR
CCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHH
NDVAALVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITCKTEHKAVLDSCRQLE
HCHHEEECCCCCEEEEECCCCCCCCEEEEECHHHHHHCCCEEEEEECHHHHHHHHHHHHH
REGFEVTYLAPQPNGIIDLKQLEATMREDTILVSIMHVNNEIGVVQDIAEIGEMCRSRGI
HCCCEEEEECCCCCCCEEHHHHHHHHCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHCCE
IFHVDATQSVGKLPIDLSKLKVDLMSFSAHKVYGPMGIGALFVRRKPRIRIEAQQHGGGH
EEEEECCCCCCCCCCCHHHHHHHHHHCCHHHEECCCCHHHHEEECCCCEEEEECCCCCCH
ERGMRSGTLPVHQIVGMGEAYRIAKEEMESEAARLRSLRLRLWNGIKDIEEVYLNGSLEN
HCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCC
GAPGILNVSFNYVEGESLIMALKDLAVSSGSACTSASLEPSYVLRALGMNDELAHSSIRF
CCCCEEEEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHH
SLGRFTTEEEIDYAIALVHKSIGRLRDLSPLWDMFKQGVDISSIEWSHH
HHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
TESKVKTPIYLDYAATTPVDPRVAEKMMQYLTLDGIFGNPASRSHKFGWQAEEAVDIAR
CCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHH
NDVAALVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITCKTEHKAVLDSCRQLE
HCHHEEECCCCCEEEEECCCCCCCCEEEEECHHHHHHCCCEEEEEECHHHHHHHHHHHHH
REGFEVTYLAPQPNGIIDLKQLEATMREDTILVSIMHVNNEIGVVQDIAEIGEMCRSRGI
HCCCEEEEECCCCCCCEEHHHHHHHHCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHCCE
IFHVDATQSVGKLPIDLSKLKVDLMSFSAHKVYGPMGIGALFVRRKPRIRIEAQQHGGGH
EEEEECCCCCCCCCCCHHHHHHHHHHCCHHHEECCCCHHHHEEECCCCEEEEECCCCCCH
ERGMRSGTLPVHQIVGMGEAYRIAKEEMESEAARLRSLRLRLWNGIKDIEEVYLNGSLEN
HCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCC
GAPGILNVSFNYVEGESLIMALKDLAVSSGSACTSASLEPSYVLRALGMNDELAHSSIRF
CCCCEEEEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHH
SLGRFTTEEEIDYAIALVHKSIGRLRDLSPLWDMFKQGVDISSIEWSHH
HHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA