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The map label for this gene is iscA [H]

Identifier: 218929953

GI number: 218929953

Start: 3237937

End: 3238260

Strand: Reverse

Name: iscA [H]

Synonym: YPO2894

Alternate gene names: 218929953

Gene position: 3238260-3237937 (Counterclockwise)

Preceding gene: 218929954

Following gene: 218929952

Centisome position: 69.58

GC content: 46.3

Gene sequence:

>324_bases
ATGTCGATATCAATCAGCGACAGTGCTGCACAGCGGGTCAGTGCCTTTTTAAATCACCGCGGTAAAGGCTTGGGTCTACG
CTTGGGCGTAAGGACTTCGGGTTGTTCCGGCATGGCATATGTACTCGAATTTGTTGACGAAATAAACGATGACGATATCG
TTTTTGAAGACAAAGGTGTGAAAGTGATCATCGACGGTAAAAGCATGGTCTATCTTGACGGTACCGAGCTGGATTTCGTC
AAAGAAGGGCTGAACGAAGGCTTTAAGTTCAACAATCCAAACGTCTCAAATGAGTGCGGATGCGGCGAAAGCTTTAACGT
TTGA

Upstream 100 bases:

>100_bases
GCTTACACAGGTAAGTCATTTGGGTGAACGAGCGCAACCAACACCTGTAGTTTGAAAGATGACGGGGATATGAGCTGGTT
CTCAGAGTTGAGGTTGTTGT

Downstream 100 bases:

>100_bases
TCGCTGCTTTTTCCTGCCCCAGATGTTTGTTGCTCTGTTCCGCTCTCTTTTGCCAAAAGGGAGCGAAACAGATCTCAGCT
TAGCCATTACAACAACTTCC

Product: iron-sulfur cluster assembly protein

Products: NA

Alternate protein names: Iron-sulfur cluster assembly protein [H]

Number of amino acids: Translated: 107; Mature: 106

Protein sequence:

>107_residues
MSISISDSAAQRVSAFLNHRGKGLGLRLGVRTSGCSGMAYVLEFVDEINDDDIVFEDKGVKVIIDGKSMVYLDGTELDFV
KEGLNEGFKFNNPNVSNECGCGESFNV

Sequences:

>Translated_107_residues
MSISISDSAAQRVSAFLNHRGKGLGLRLGVRTSGCSGMAYVLEFVDEINDDDIVFEDKGVKVIIDGKSMVYLDGTELDFV
KEGLNEGFKFNNPNVSNECGCGESFNV
>Mature_106_residues
SISISDSAAQRVSAFLNHRGKGLGLRLGVRTSGCSGMAYVLEFVDEINDDDIVFEDKGVKVIIDGKSMVYLDGTELDFVK
EGLNEGFKFNNPNVSNECGCGESFNV

Specific function: Is able to transfer iron-sulfur clusters to apo- ferredoxin. Multiple cycles of [2Fe2S] cluster formation and transfer are observed, suggesting that iscA acts catalytically. Recruits intracellular free iron so as to provide iron for the assembly of transi

COG id: COG0316

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesB/iscA family [H]

Homologues:

Organism=Homo sapiens, GI52426768, Length=106, Percent_Identity=39.622641509434, Blast_Score=99, Evalue=1e-21,
Organism=Homo sapiens, GI160420328, Length=106, Percent_Identity=36.7924528301887, Blast_Score=73, Evalue=6e-14,
Organism=Escherichia coli, GI1788877, Length=107, Percent_Identity=79.4392523364486, Blast_Score=179, Evalue=4e-47,
Organism=Escherichia coli, GI1787974, Length=105, Percent_Identity=45.7142857142857, Blast_Score=112, Evalue=6e-27,
Organism=Escherichia coli, GI1786351, Length=110, Percent_Identity=36.3636363636364, Blast_Score=74, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI25146411, Length=106, Percent_Identity=35.8490566037736, Blast_Score=89, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI193209025, Length=106, Percent_Identity=35.8490566037736, Blast_Score=89, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI71998452, Length=106, Percent_Identity=33.0188679245283, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323001, Length=105, Percent_Identity=39.0476190476191, Blast_Score=85, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24642263, Length=107, Percent_Identity=41.1214953271028, Blast_Score=94, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24649697, Length=108, Percent_Identity=32.4074074074074, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000361
- InterPro:   IPR016092
- InterPro:   IPR017870
- InterPro:   IPR011302 [H]

Pfam domain/function: PF01521 Fe-S_biosyn [H]

EC number: NA

Molecular weight: Translated: 11589; Mature: 11458

Theoretical pI: Translated: 4.28; Mature: 4.28

Prosite motif: PS01152 HESB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSISISDSAAQRVSAFLNHRGKGLGLRLGVRTSGCSGMAYVLEFVDEINDDDIVFEDKGV
CEEECCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCEEEECCCC
KVIIDGKSMVYLDGTELDFVKEGLNEGFKFNNPNVSNECGCGESFNV
EEEECCCEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SISISDSAAQRVSAFLNHRGKGLGLRLGVRTSGCSGMAYVLEFVDEINDDDIVFEDKGV
EEECCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCEEEECCCC
KVIIDGKSMVYLDGTELDFVKEGLNEGFKFNNPNVSNECGCGESFNV
EEEECCCEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA