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The map label for this gene is phrB [H]

Identifier: 218929768

GI number: 218929768

Start: 3022620

End: 3024083

Strand: Direct

Name: phrB [H]

Synonym: YPO2695

Alternate gene names: 218929768

Gene position: 3022620-3024083 (Clockwise)

Preceding gene: 218929767

Following gene: 218929769

Centisome position: 64.95

GC content: 51.09

Gene sequence:

>1464_bases
ATGTCTACGCATCTGGTCTGGTTCCGCAATGATTTACGGGTCACCGATAATTTGGCGCTTCATGCGGCCTGTCAGGACCC
ACAAGCGAGCGTAATTGCCGTGTTTATTGCCACACCCGTGCAATGGGCTGCACATGGTATGGCACCCAGGCAGGCGGCTT
TCCTGCGACAAAATTTACAACATTTGCAGGCAGCTCTCGCGGCTCGAGGGATACCGCTGCATTATCACCAATGTGATGAT
TTTCAGGCGTCAAATACTTGGCTAGTCGATTTTTGTCAGCAACAGCAGGTCGATAGCCTGTTTTATTCTCAACAGTATGA
ACTGAACGAGCGGTTACGTGACGATGCGCTGGCGGTGCAACTCAGTGAGCGAGCTATCACCTGCCAGTCTTTTCATGACA
GTGTATTGCTCGCGCCGGGGACGGTTCTGACCGGTAATAATGAAATGTATAAAGTGTTTACGCCTTTTCGCCGTGCGTTT
ATTCAGCGTTTATTGATGAGTGATAACCACTGTGTTCCGGCCCCGAAAGCCCGCCATGCTCCTCCCGTTCTGCTCTCACC
GTTGCCTTTTGATTACCCACAGCAGTCCGTTGATAGCCAGTTATTTCCTGCAGGGGAAGACGCTGCCTTACAGCGTTTAC
GCCGTTTTTGTCGTGAACAGGTACAGGATTACCGGCAATACCGCGATTTGCCGGCGGTGCCCGGTACCAGTTGCTTATCC
CCTTATTTGGTGTTGGGGGTCCTTTCACCGCGTCAATGTTTTAATCGCCTGTGGGCTGAATCCCCAGAAATGTTAGAGAA
CGCAGACAGTGGTGCATTCACTTGGCTTAATGAGCTAATCTGGCGTGAGTTTTATCGCCATTTATTAGTGGCCTATCCCC
GTTTATGCCAACATCATCCCTTTATTGGCTGGACTGATGGGTTAGTATGGAACCGATCTGAATCGCAATTGAAAGCCTGG
CAGCAGGGGCAAACCGGTTATCCCATCGTTGATGCCGCGATGCGTCAAATGAATGAAACTGGCTGGATGCATAATCGCCT
TCGCATGATCAGCGCCAGTTTTTTGGTTAAAGACCTGTTGATCGACTGGCGGCAAGGTGAGCGTTATTTTATGTCACAAT
TGGTGGATGGGGACTTAGCCGCGAATAATGGCGGCTGGCAATGGGCCGCATCAACCGGTACCGATGCCGCACCGTATTTT
CGTATTTTTAATCCCACCACGCAGGGCGAGCGGTTTGATAAGGACGGGGTATTTATTCGTCGTTGGTTACCGGAGCTGGC
TGCGGTACCAGACAGTGATATTCATCAGCCATGGCGCTGGGCAGAACGGCAACAGCAGCATCTGGATTATCCTGTCCCGT
TGGTTGATCACAAGCAAGCCAGATTGGCTACACTCGCGGCATTTGAAGCCGCTAAGCGGGATAGCACAAGGATCCCTAAT
CGCAAGGAGCCTTCATGTTATTGA

Upstream 100 bases:

>100_bases
AAGTTACGCACAGGATTAAGTACCAAGCAGGCCGTTGTCTGGTGGTTGAATGAAATTTAACCCTGAAGACGTTGAGCCCA
TGAGTAAATAAGGATAAGGC

Downstream 100 bases:

>100_bases
AATATCTCTCGATTAAATCTGCATCAGATAAGATAAAGAGTGGTCTGCTAAAGACGGGCGTGATTTTATCTTTCAGTCTC
TTCAGTTCACTGTCCACTGC

Product: deoxyribodipyrimidine photolyase

Products: NA

Alternate protein names: DNA photolyase; Photoreactivating enzyme [H]

Number of amino acids: Translated: 487; Mature: 486

Protein sequence:

>487_residues
MSTHLVWFRNDLRVTDNLALHAACQDPQASVIAVFIATPVQWAAHGMAPRQAAFLRQNLQHLQAALAARGIPLHYHQCDD
FQASNTWLVDFCQQQQVDSLFYSQQYELNERLRDDALAVQLSERAITCQSFHDSVLLAPGTVLTGNNEMYKVFTPFRRAF
IQRLLMSDNHCVPAPKARHAPPVLLSPLPFDYPQQSVDSQLFPAGEDAALQRLRRFCREQVQDYRQYRDLPAVPGTSCLS
PYLVLGVLSPRQCFNRLWAESPEMLENADSGAFTWLNELIWREFYRHLLVAYPRLCQHHPFIGWTDGLVWNRSESQLKAW
QQGQTGYPIVDAAMRQMNETGWMHNRLRMISASFLVKDLLIDWRQGERYFMSQLVDGDLAANNGGWQWAASTGTDAAPYF
RIFNPTTQGERFDKDGVFIRRWLPELAAVPDSDIHQPWRWAERQQQHLDYPVPLVDHKQARLATLAAFEAAKRDSTRIPN
RKEPSCY

Sequences:

>Translated_487_residues
MSTHLVWFRNDLRVTDNLALHAACQDPQASVIAVFIATPVQWAAHGMAPRQAAFLRQNLQHLQAALAARGIPLHYHQCDD
FQASNTWLVDFCQQQQVDSLFYSQQYELNERLRDDALAVQLSERAITCQSFHDSVLLAPGTVLTGNNEMYKVFTPFRRAF
IQRLLMSDNHCVPAPKARHAPPVLLSPLPFDYPQQSVDSQLFPAGEDAALQRLRRFCREQVQDYRQYRDLPAVPGTSCLS
PYLVLGVLSPRQCFNRLWAESPEMLENADSGAFTWLNELIWREFYRHLLVAYPRLCQHHPFIGWTDGLVWNRSESQLKAW
QQGQTGYPIVDAAMRQMNETGWMHNRLRMISASFLVKDLLIDWRQGERYFMSQLVDGDLAANNGGWQWAASTGTDAAPYF
RIFNPTTQGERFDKDGVFIRRWLPELAAVPDSDIHQPWRWAERQQQHLDYPVPLVDHKQARLATLAAFEAAKRDSTRIPN
RKEPSCY
>Mature_486_residues
STHLVWFRNDLRVTDNLALHAACQDPQASVIAVFIATPVQWAAHGMAPRQAAFLRQNLQHLQAALAARGIPLHYHQCDDF
QASNTWLVDFCQQQQVDSLFYSQQYELNERLRDDALAVQLSERAITCQSFHDSVLLAPGTVLTGNNEMYKVFTPFRRAFI
QRLLMSDNHCVPAPKARHAPPVLLSPLPFDYPQQSVDSQLFPAGEDAALQRLRRFCREQVQDYRQYRDLPAVPGTSCLSP
YLVLGVLSPRQCFNRLWAESPEMLENADSGAFTWLNELIWREFYRHLLVAYPRLCQHHPFIGWTDGLVWNRSESQLKAWQ
QGQTGYPIVDAAMRQMNETGWMHNRLRMISASFLVKDLLIDWRQGERYFMSQLVDGDLAANNGGWQWAASTGTDAAPYFR
IFNPTTQGERFDKDGVFIRRWLPELAAVPDSDIHQPWRWAERQQQHLDYPVPLVDHKQARLATLAAFEAAKRDSTRIPNR
KEPSCY

Specific function: Involved in repair of UV radiation-induced DNA damage. Catalyzes the light-dependent monomerization (300-600 nm) of cyclobutyl pyrimidine dimers (in cis-syn configuration), which are formed between adjacent bases on the same DNA strand upon exposure to ul

COG id: COG0415

COG function: function code L; Deoxyribodipyrimidine photolyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DNA photolyase domain [H]

Homologues:

Organism=Homo sapiens, GI188536100, Length=489, Percent_Identity=26.7893660531697, Blast_Score=153, Evalue=3e-37,
Organism=Homo sapiens, GI188536103, Length=286, Percent_Identity=31.8181818181818, Blast_Score=142, Evalue=6e-34,
Organism=Homo sapiens, GI4758072, Length=485, Percent_Identity=26.8041237113402, Blast_Score=139, Evalue=5e-33,
Organism=Escherichia coli, GI1786926, Length=475, Percent_Identity=60.2105263157895, Blast_Score=595, Evalue=1e-171,
Organism=Saccharomyces cerevisiae, GI6324962, Length=512, Percent_Identity=31.640625, Blast_Score=258, Evalue=2e-69,
Organism=Drosophila melanogaster, GI17137248, Length=507, Percent_Identity=26.4299802761341, Blast_Score=152, Evalue=6e-37,
Organism=Drosophila melanogaster, GI24585455, Length=507, Percent_Identity=26.4299802761341, Blast_Score=152, Evalue=6e-37,
Organism=Drosophila melanogaster, GI24648152, Length=543, Percent_Identity=25.414364640884, Blast_Score=123, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002081
- InterPro:   IPR018394
- InterPro:   IPR006050
- InterPro:   IPR005101
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00875 DNA_photolyase; PF03441 FAD_binding_7 [H]

EC number: =4.1.99.3 [H]

Molecular weight: Translated: 56067; Mature: 55936

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: PS00394 DNA_PHOTOLYASES_1_1 ; PS00691 DNA_PHOTOLYASES_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTHLVWFRNDLRVTDNLALHAACQDPQASVIAVFIATPVQWAAHGMAPRQAAFLRQNLQ
CCCEEEEEECCCEECCCEEEEEECCCCCCCEEEEEECCCHHHHHCCCCHHHHHHHHHHHH
HLQAALAARGIPLHYHQCDDFQASNTWLVDFCQQQQVDSLFYSQQYELNERLRDDALAVQ
HHHHHHHHCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEE
LSERAITCQSFHDSVLLAPGTVLTGNNEMYKVFTPFRRAFIQRLLMSDNHCVPAPKARHA
HHHHHEEHHHHCCCEEECCCEEEECCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
PPVLLSPLPFDYPQQSVDSQLFPAGEDAALQRLRRFCREQVQDYRQYRDLPAVPGTSCLS
CCCEECCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
PYLVLGVLSPRQCFNRLWAESPEMLENADSGAFTWLNELIWREFYRHLLVAYPRLCQHHP
HHHHHHCCCHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
FIGWTDGLVWNRSESQLKAWQQGQTGYPIVDAAMRQMNETGWMHNRLRMISASFLVKDLL
CCEECCCEEECCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IDWRQGERYFMSQLVDGDLAANNGGWQWAASTGTDAAPYFRIFNPTTQGERFDKDGVFIR
HHHHCCHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCEEEEECCCCCCCCCCCCCHHHH
RWLPELAAVPDSDIHQPWRWAERQQQHLDYPVPLVDHKQARLATLAAFEAAKRDSTRIPN
HHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCC
RKEPSCY
CCCCCCC
>Mature Secondary Structure 
STHLVWFRNDLRVTDNLALHAACQDPQASVIAVFIATPVQWAAHGMAPRQAAFLRQNLQ
CCEEEEEECCCEECCCEEEEEECCCCCCCEEEEEECCCHHHHHCCCCHHHHHHHHHHHH
HLQAALAARGIPLHYHQCDDFQASNTWLVDFCQQQQVDSLFYSQQYELNERLRDDALAVQ
HHHHHHHHCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEE
LSERAITCQSFHDSVLLAPGTVLTGNNEMYKVFTPFRRAFIQRLLMSDNHCVPAPKARHA
HHHHHEEHHHHCCCEEECCCEEEECCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
PPVLLSPLPFDYPQQSVDSQLFPAGEDAALQRLRRFCREQVQDYRQYRDLPAVPGTSCLS
CCCEECCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
PYLVLGVLSPRQCFNRLWAESPEMLENADSGAFTWLNELIWREFYRHLLVAYPRLCQHHP
HHHHHHCCCHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
FIGWTDGLVWNRSESQLKAWQQGQTGYPIVDAAMRQMNETGWMHNRLRMISASFLVKDLL
CCEECCCEEECCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IDWRQGERYFMSQLVDGDLAANNGGWQWAASTGTDAAPYFRIFNPTTQGERFDKDGVFIR
HHHHCCHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCEEEEECCCCCCCCCCCCCHHHH
RWLPELAAVPDSDIHQPWRWAERQQQHLDYPVPLVDHKQARLATLAAFEAAKRDSTRIPN
HHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCC
RKEPSCY
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1840665; 11677609 [H]