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The map label for this gene is insB [H]

Identifier: 218928134

GI number: 218928134

Start: 1065829

End: 1066185

Strand: Reverse

Name: insB [H]

Synonym: YPO0963

Alternate gene names: 218928134

Gene position: 1066185-1065829 (Counterclockwise)

Preceding gene: 218928135

Following gene: 218928133

Centisome position: 22.91

GC content: 46.78

Gene sequence:

>357_bases
GTGGGCAACAAAAAGCAGCAACGTTGGCTTTGGTATGCCTGGGAGTCTCGCCTCAAACGTATTATTGCTCATGTTTTTGG
TCGTCGGAGCAAAAAGACATTTCGCCAATTATTGGGGTTGCTGTCAGGTTTCAATATCGTCTTCTGGTGTACCGACAACT
TCAGTGCTTATGAGATGTTGCCGGATGAAAAACACATCAGGAGCAAGCTTTACACGCAGCGGATTGAGCGTGAAAACCTG
AATATCCGTAATCGGTTGAAACGACTGAACCGCAAGACTCTCGGAGACTCAAAGTCGGCAGAAATGCATGACAGGATCAT
CGGTACGTTCATTGAGCGTGAGCATTACCTAGTGTGA

Upstream 100 bases:

>100_bases
TGCGCACTTTAAAAAACTCTCGCCGCAGCGTGTAACCACGCTCCCACTGGATAACCTGCAAATTCAGCTTATCTGTGAAG
TCGATGAAATGTGGTCATTT

Downstream 100 bases:

>100_bases
TACTAATAACCAGATTGAATACATGACCGAATTTTTATTTTTATGGTGTTTGTCTGTGGGTGCGTGTCAACTATAACCTT
TGAACCGAGTGAATTGGCGG

Product: insertion sequence protein

Products: NA

Alternate protein names: IS1a [H]

Number of amino acids: Translated: 118; Mature: 117

Protein sequence:

>118_residues
MGNKKQQRWLWYAWESRLKRIIAHVFGRRSKKTFRQLLGLLSGFNIVFWCTDNFSAYEMLPDEKHIRSKLYTQRIERENL
NIRNRLKRLNRKTLGDSKSAEMHDRIIGTFIEREHYLV

Sequences:

>Translated_118_residues
MGNKKQQRWLWYAWESRLKRIIAHVFGRRSKKTFRQLLGLLSGFNIVFWCTDNFSAYEMLPDEKHIRSKLYTQRIERENL
NIRNRLKRLNRKTLGDSKSAEMHDRIIGTFIEREHYLV
>Mature_117_residues
GNKKQQRWLWYAWESRLKRIIAHVFGRRSKKTFRQLLGLLSGFNIVFWCTDNFSAYEMLPDEKHIRSKLYTQRIERENLN
IRNRLKRLNRKTLGDSKSAEMHDRIIGTFIEREHYLV

Specific function: Absolutely required for transposition of IS1 [H]

COG id: COG1662

COG function: function code L; Transposase and inactivated derivatives, IS1 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 27 family [H]

Homologues:

Organism=Escherichia coli, GI1789853, Length=117, Percent_Identity=50.4273504273504, Blast_Score=121, Evalue=1e-29,
Organism=Escherichia coli, GI1788203, Length=117, Percent_Identity=50.4273504273504, Blast_Score=121, Evalue=1e-29,
Organism=Escherichia coli, GI1786203, Length=117, Percent_Identity=50.4273504273504, Blast_Score=121, Evalue=1e-29,
Organism=Escherichia coli, GI1787223, Length=117, Percent_Identity=49.5726495726496, Blast_Score=118, Evalue=1e-28,
Organism=Escherichia coli, GI1786470, Length=117, Percent_Identity=49.5726495726496, Blast_Score=117, Evalue=2e-28,
Organism=Escherichia coli, GI1786459, Length=117, Percent_Identity=49.5726495726496, Blast_Score=117, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005063 [H]

Pfam domain/function: PF03400 Transposase_27 [H]

EC number: NA

Molecular weight: Translated: 14399; Mature: 14268

Theoretical pI: Translated: 11.12; Mature: 11.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNKKQQRWLWYAWESRLKRIIAHVFGRRSKKTFRQLLGLLSGFNIVFWCTDNFSAYEML
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHCC
PDEKHIRSKLYTQRIERENLNIRNRLKRLNRKTLGDSKSAEMHDRIIGTFIEREHYLV
CCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
GNKKQQRWLWYAWESRLKRIIAHVFGRRSKKTFRQLLGLLSGFNIVFWCTDNFSAYEML
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHCC
PDEKHIRSKLYTQRIERENLNIRNRLKRLNRKTLGDSKSAEMHDRIIGTFIEREHYLV
CCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 375010; 1849492; 1630901; 9278503 [H]