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The map label for this gene is rio1 [H]

Identifier: 218884398

GI number: 218884398

Start: 1026643

End: 1027446

Strand: Direct

Name: rio1 [H]

Synonym: DKAM_1087

Alternate gene names: 218884398

Gene position: 1026643-1027446 (Clockwise)

Preceding gene: 218884397

Following gene: 218884399

Centisome position: 75.2

GC content: 40.8

Gene sequence:

>804_bases
ATGAGCGACGACATAGATAAATTACTCAGGAAAAAGGAGGAGCCCCGTAGAAAAGATAAAGACCTCTTTGAAACGGTTGA
AGAGGTCTTTGATACAGCCACAGTCATGACTATCATTGAATTAATAAGGAAGAAAATAATTAAGAAGCTAAATGGTGTCG
TGAACACTGGTAAAGAGGCCAGGGTGTATCTTGGAATAGGGTATAATGGAGAATACCTAGCCGTGAAGATATATTTAACT
AGCACGGCAGAGTTCAAGAAGGGAATATACAAGTATATCATGGGGGATCCACGCTTTAGAGACGTCAAGATCAAGGATAC
AAGGACACTTGTATATGTTTGGACAAGGAAAGAATACAGAAACCTGAAAAGACTCTATGAAGCAGGGGTAAAGGTCCCCA
GGCCCGTGGCATTCCTGAACAATGTCTTAGTAATGGAGTTCCTTGGCGAGAACGGAAATAGATATCCACTCCTTATAGAA
GCCTACAAGGAGCTGGAAGTTGAAGAGCTCAAGCACGTTTACCACCTTATATTAGATGAGGTAGTGAAAATATATTGTAA
AGCCGGGTTAGTACACGGTGATCTCTCAGTGTATAATATAGTTGTGACCCCAGGGCTCGACATAGCTATAATAGATGTTG
GCCAAGCAGTGGATTTATCCCATCCAAACTCCGAGGAATTCTTAATCAGGGACATCGAGAATATAAACAGGTTTTTCAGG
GAGGAAACAGGTATAAGTACATATAGTCTAGAGGAAATACTGGAGGCTGTTAAAGAATGCAGGACAATGAAAAAGGAAGA
TTAA

Upstream 100 bases:

>100_bases
AAGGTGAAGTAGTATATAAGTACGGTAAGAACGAGGTAAACAAACTGGTTGAGAAGGGGGTAGTGCCGAAGGAGTTCATC
GATGCATTAAGTGAACTCAT

Downstream 100 bases:

>100_bases
TTCCTGGAGTAACTAGGCTCTACGAGAAACTCCCCTTGGAAAGGATTGGAGTCCTTCTCGGTGATAACGGGAGAGTTAAG
AAGGAGATAGAGGTTAAGAC

Product: putative kinase

Products: NA

Alternate protein names: AfRio1 [H]

Number of amino acids: Translated: 267; Mature: 266

Protein sequence:

>267_residues
MSDDIDKLLRKKEEPRRKDKDLFETVEEVFDTATVMTIIELIRKKIIKKLNGVVNTGKEARVYLGIGYNGEYLAVKIYLT
STAEFKKGIYKYIMGDPRFRDVKIKDTRTLVYVWTRKEYRNLKRLYEAGVKVPRPVAFLNNVLVMEFLGENGNRYPLLIE
AYKELEVEELKHVYHLILDEVVKIYCKAGLVHGDLSVYNIVVTPGLDIAIIDVGQAVDLSHPNSEEFLIRDIENINRFFR
EETGISTYSLEEILEAVKECRTMKKED

Sequences:

>Translated_267_residues
MSDDIDKLLRKKEEPRRKDKDLFETVEEVFDTATVMTIIELIRKKIIKKLNGVVNTGKEARVYLGIGYNGEYLAVKIYLT
STAEFKKGIYKYIMGDPRFRDVKIKDTRTLVYVWTRKEYRNLKRLYEAGVKVPRPVAFLNNVLVMEFLGENGNRYPLLIE
AYKELEVEELKHVYHLILDEVVKIYCKAGLVHGDLSVYNIVVTPGLDIAIIDVGQAVDLSHPNSEEFLIRDIENINRFFR
EETGISTYSLEEILEAVKECRTMKKED
>Mature_266_residues
SDDIDKLLRKKEEPRRKDKDLFETVEEVFDTATVMTIIELIRKKIIKKLNGVVNTGKEARVYLGIGYNGEYLAVKIYLTS
TAEFKKGIYKYIMGDPRFRDVKIKDTRTLVYVWTRKEYRNLKRLYEAGVKVPRPVAFLNNVLVMEFLGENGNRYPLLIEA
YKELEVEELKHVYHLILDEVVKIYCKAGLVHGDLSVYNIVVTPGLDIAIIDVGQAVDLSHPNSEEFLIRDIENINRFFRE
ETGISTYSLEEILEAVKECRTMKKED

Specific function: Autophosphorylation of the rio1 protein is not necessary for maintenance of kinase activity. Prefers ATP over GTP. The yeast ortholog is involved in ribosome biogenesis [H]

COG id: COG1718

COG function: function code TD; Serine/threonine protein kinase involved in cell cycle control

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 protein kinase domain [H]

Homologues:

Organism=Homo sapiens, GI23510356, Length=254, Percent_Identity=36.6141732283465, Blast_Score=152, Evalue=4e-37,
Organism=Homo sapiens, GI22325377, Length=254, Percent_Identity=32.2834645669291, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI23510358, Length=148, Percent_Identity=35.1351351351351, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI229331971, Length=208, Percent_Identity=27.4038461538462, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI229331967, Length=208, Percent_Identity=27.4038461538462, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI115532073, Length=243, Percent_Identity=38.6831275720165, Blast_Score=159, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17556977, Length=240, Percent_Identity=35.8333333333333, Blast_Score=149, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI71989043, Length=147, Percent_Identity=40.8163265306122, Blast_Score=104, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI32564178, Length=210, Percent_Identity=24.7619047619048, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6324693, Length=265, Percent_Identity=37.3584905660377, Blast_Score=141, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6324122, Length=217, Percent_Identity=22.5806451612903, Blast_Score=63, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24662811, Length=260, Percent_Identity=37.6923076923077, Blast_Score=157, Evalue=6e-39,
Organism=Drosophila melanogaster, GI21356879, Length=260, Percent_Identity=37.6923076923077, Blast_Score=157, Evalue=6e-39,
Organism=Drosophila melanogaster, GI19920700, Length=278, Percent_Identity=33.8129496402878, Blast_Score=125, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24649986, Length=237, Percent_Identity=22.3628691983122, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011009
- InterPro:   IPR000719
- InterPro:   IPR018934
- InterPro:   IPR000687
- InterPro:   IPR018935 [H]

Pfam domain/function: PF01163 RIO1 [H]

EC number: =2.7.11.1 [H]

Molecular weight: Translated: 31036; Mature: 30905

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: PS00109 PROTEIN_KINASE_TYR ; PS50011 PROTEIN_KINASE_DOM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDDIDKLLRKKEEPRRKDKDLFETVEEVFDTATVMTIIELIRKKIIKKLNGVVNTGKEA
CCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
RVYLGIGYNGEYLAVKIYLTSTAEFKKGIYKYIMGDPRFRDVKIKDTRTLVYVWTRKEYR
EEEEEECCCCCEEEEEEEEECCHHHHHHHHHHHCCCCCEEEEEEECCEEEEEEEECHHHH
NLKRLYEAGVKVPRPVAFLNNVLVMEFLGENGNRYPLLIEAYKELEVEELKHVYHLILDE
HHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHCCHHHHHHHHHHHHHH
VVKIYCKAGLVHGDLSVYNIVVTPGLDIAIIDVGQAVDLSHPNSEEFLIRDIENINRFFR
HHHHHHHHCCEECCCEEEEEEEECCCCEEEEECCCEEECCCCCCHHHHHHHHHHHHHHHH
EETGISTYSLEEILEAVKECRTMKKED
HHCCCCHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SDDIDKLLRKKEEPRRKDKDLFETVEEVFDTATVMTIIELIRKKIIKKLNGVVNTGKEA
CCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
RVYLGIGYNGEYLAVKIYLTSTAEFKKGIYKYIMGDPRFRDVKIKDTRTLVYVWTRKEYR
EEEEEECCCCCEEEEEEEEECCHHHHHHHHHHHCCCCCEEEEEEECCEEEEEEEECHHHH
NLKRLYEAGVKVPRPVAFLNNVLVMEFLGENGNRYPLLIEAYKELEVEELKHVYHLILDE
HHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHCCHHHHHHHHHHHHHH
VVKIYCKAGLVHGDLSVYNIVVTPGLDIAIIDVGQAVDLSHPNSEEFLIRDIENINRFFR
HHHHHHHHCCEECCCEEEEEEEECCCCEEEEECCCEEECCCCCCHHHHHHHHHHHHHHHH
EETGISTYSLEEILEAVKECRTMKKED
HHCCCCHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]