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The map label for this gene is hycF [H]

Identifier: 218884333

GI number: 218884333

Start: 972331

End: 972846

Strand: Direct

Name: hycF [H]

Synonym: DKAM_1022

Alternate gene names: 218884333

Gene position: 972331-972846 (Clockwise)

Preceding gene: 218884332

Following gene: 218884334

Centisome position: 71.22

GC content: 44.57

Gene sequence:

>516_bases
ATGAGTAAACTCCTTAAACCAGTTAAACTAGTAGTAATAGCTGGTGAAGCAGGGAGAGTAACAAGGAAGTATCCGTTTGA
AGAACCACTTGTAACACCGGACTTCAGGGGTAAAATAGAGATAAATGCCTCCAAATGCATTGGATGCGGTGCATGCGTTA
ATGCATGCCCACCAAACGCGTTACAGTTAATAGAGGATAAGGAGAAACTAGTGCTAAGATATTTCATCGGGAGATGCATA
TTCTGCTGGAGATGCATAGACGTATGTCCTACAGGCGCAATCAAGGGAACCCGGGAGTTCGAGCTGGCTACAGATGATAT
TTCAGACCTCTACACGCACGTAGTACACTCAAGGAAAGCATGTGCATCATGCGGCAATACCTATGCAACAGAGAAAATAA
TACGCTATGTAGCGGAGCACGCGTCCTTAAGTGAGGAATACCTGGATAAATGCCCTGATTGCAGGAAAAGAAACTTCATT
AAGGCATTATCAAGGAGGAGGGGCGGCTTTGAATAG

Upstream 100 bases:

>100_bases
TAGCCAGTATAGACCCATGCTTCTCATGTACTGATAGAATCATGGTTATAAGAGAGAATGGAAGAGTGGAGAGAATTAGT
TTTAACAGGGGTGTTCCACT

Downstream 100 bases:

>100_bases
TCTCTTAAAAAAGAGTATATGGGTATTCCATTTAAACACAGGTAGCTGTAATGCCTGCGATATAGAAATACTCGACGCCT
TGACCCCGTTCCATGATGCA

Product: Hydrogenase 4 Fe-S subunit

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: FHL subunit 6; Hydrogenase-3 component F [H]

Number of amino acids: Translated: 171; Mature: 170

Protein sequence:

>171_residues
MSKLLKPVKLVVIAGEAGRVTRKYPFEEPLVTPDFRGKIEINASKCIGCGACVNACPPNALQLIEDKEKLVLRYFIGRCI
FCWRCIDVCPTGAIKGTREFELATDDISDLYTHVVHSRKACASCGNTYATEKIIRYVAEHASLSEEYLDKCPDCRKRNFI
KALSRRRGGFE

Sequences:

>Translated_171_residues
MSKLLKPVKLVVIAGEAGRVTRKYPFEEPLVTPDFRGKIEINASKCIGCGACVNACPPNALQLIEDKEKLVLRYFIGRCI
FCWRCIDVCPTGAIKGTREFELATDDISDLYTHVVHSRKACASCGNTYATEKIIRYVAEHASLSEEYLDKCPDCRKRNFI
KALSRRRGGFE
>Mature_170_residues
SKLLKPVKLVVIAGEAGRVTRKYPFEEPLVTPDFRGKIEINASKCIGCGACVNACPPNALQLIEDKEKLVLRYFIGRCIF
CWRCIDVCPTGAIKGTREFELATDDISDLYTHVVHSRKACASCGNTYATEKIIRYVAEHASLSEEYLDKCPDCRKRNFIK
ALSRRRGGFE

Specific function: Probable electron transfer protein for hydrogenase 3 [H]

COG id: COG1143

COG function: function code C; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1789075, Length=152, Percent_Identity=38.1578947368421, Blast_Score=103, Evalue=6e-24,
Organism=Escherichia coli, GI1788833, Length=162, Percent_Identity=39.5061728395062, Blast_Score=85, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012285 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: NA

Molecular weight: Translated: 19211; Mature: 19079

Theoretical pI: Translated: 8.55; Mature: 8.55

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

7.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
7.6 %Cys+Met (Translated Protein)
7.1 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLLKPVKLVVIAGEAGRVTRKYPFEEPLVTPDFRGKIEINASKCIGCGACVNACPPNA
CCCHHCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEECHHHHHCCHHHHHCCCCHH
LQLIEDKEKLVLRYFIGRCIFCWRCIDVCPTGAIKGTREFELATDDISDLYTHVVHSRKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHH
CASCGNTYATEKIIRYVAEHASLSEEYLDKCPDCRKRNFIKALSRRRGGFE
HHHCCCHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SKLLKPVKLVVIAGEAGRVTRKYPFEEPLVTPDFRGKIEINASKCIGCGACVNACPPNA
CCHHCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEECHHHHHCCHHHHHCCCCHH
LQLIEDKEKLVLRYFIGRCIFCWRCIDVCPTGAIKGTREFELATDDISDLYTHVVHSRKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHH
CASCGNTYATEKIIRYVAEHASLSEEYLDKCPDCRKRNFIKALSRRRGGFE
HHHCCCHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503 [H]