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The map label for this gene is hycG [H]

Identifier: 218884334

GI number: 218884334

Start: 972839

End: 973309

Strand: Direct

Name: hycG [H]

Synonym: DKAM_1023

Alternate gene names: 218884334

Gene position: 972839-973309 (Clockwise)

Preceding gene: 218884333

Following gene: 218884336

Centisome position: 71.26

GC content: 46.28

Gene sequence:

>471_bases
TTGAATAGTCTCTTAAAAAAGAGTATATGGGTATTCCATTTAAACACAGGTAGCTGTAATGCCTGCGATATAGAAATACT
CGACGCCTTGACCCCGTTCCATGATGCAGAGAGATTCGGTGTTAAACTAGTAGGGTCCCCGAGGCACGCTGACGTGATAT
TGTTAACAGGGCCAATAACTATTGAAACGCTCCCTAAAGTAATAAACGCTATAGAGGCTGCACCGCGCCCAAGAATAATA
GTGGCACTAGGATCCTGCGGTGTTGGAGGCGGTATATGGCATGACACGTATTCAACCATCGGAGGCGTGAGGAGCCTGAA
AAAAATCCTGGATGAAAAGGGTATACAGGTCGACGGGATATACTATGTGCCAGGATGCCCTATAAGGCCTGAAGCCCTCC
TATATGCTATCGCGTTGATAAAGGGACTTGTATCAAAGAAGGTGAAGGGGGAGATAAAGTACGCCGAGTAA

Upstream 100 bases:

>100_bases
GTAGCGGAGCACGCGTCCTTAAGTGAGGAATACCTGGATAAATGCCCTGATTGCAGGAAAAGAAACTTCATTAAGGCATT
ATCAAGGAGGAGGGGCGGCT

Downstream 100 bases:

>100_bases
GCTTGCCGCATATATATGTAAATACATACATCATGAAACATTATTCCCCCGGCACTTCACGGGAGATATATCATCATGCA
GGAGAATCAAGGAACTAAGC

Product: NADH ubiquinone oxidoreductase, 20 kDa subunit

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: FHL subunit 7; Hydrogenase-3 component G [H]

Number of amino acids: Translated: 156; Mature: 156

Protein sequence:

>156_residues
MNSLLKKSIWVFHLNTGSCNACDIEILDALTPFHDAERFGVKLVGSPRHADVILLTGPITIETLPKVINAIEAAPRPRII
VALGSCGVGGGIWHDTYSTIGGVRSLKKILDEKGIQVDGIYYVPGCPIRPEALLYAIALIKGLVSKKVKGEIKYAE

Sequences:

>Translated_156_residues
MNSLLKKSIWVFHLNTGSCNACDIEILDALTPFHDAERFGVKLVGSPRHADVILLTGPITIETLPKVINAIEAAPRPRII
VALGSCGVGGGIWHDTYSTIGGVRSLKKILDEKGIQVDGIYYVPGCPIRPEALLYAIALIKGLVSKKVKGEIKYAE
>Mature_156_residues
MNSLLKKSIWVFHLNTGSCNACDIEILDALTPFHDAERFGVKLVGSPRHADVILLTGPITIETLPKVINAIEAAPRPRII
VALGSCGVGGGIWHDTYSTIGGVRSLKKILDEKGIQVDGIYYVPGCPIRPEALLYAIALIKGLVSKKVKGEIKYAE

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG3260

COG function: function code C; Ni,Fe-hydrogenase III small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=154, Percent_Identity=31.8181818181818, Blast_Score=93, Evalue=1e-19,
Organism=Escherichia coli, GI1789074, Length=144, Percent_Identity=43.0555555555556, Blast_Score=129, Evalue=8e-32,
Organism=Escherichia coli, GI1788834, Length=146, Percent_Identity=42.4657534246575, Blast_Score=129, Evalue=1e-31,
Organism=Escherichia coli, GI1788624, Length=145, Percent_Identity=33.1034482758621, Blast_Score=84, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17509685, Length=154, Percent_Identity=31.1688311688312, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI18859983, Length=149, Percent_Identity=33.5570469798658, Blast_Score=91, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24642371, Length=149, Percent_Identity=33.5570469798658, Blast_Score=91, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24651058, Length=156, Percent_Identity=31.4102564102564, Blast_Score=88, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: NA

Molecular weight: Translated: 16833; Mature: 16833

Theoretical pI: Translated: 8.62; Mature: 8.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSLLKKSIWVFHLNTGSCNACDIEILDALTPFHDAERFGVKLVGSPRHADVILLTGPIT
CCCHHHCCEEEEEECCCCCCCCCHHHHHHHCCCCCHHHCCEEEECCCCCCCEEEEECCCC
IETLPKVINAIEAAPRPRIIVALGSCGVGGGIWHDTYSTIGGVRSLKKILDEKGIQVDGI
HHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEECCE
YYVPGCPIRPEALLYAIALIKGLVSKKVKGEIKYAE
EECCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEECC
>Mature Secondary Structure
MNSLLKKSIWVFHLNTGSCNACDIEILDALTPFHDAERFGVKLVGSPRHADVILLTGPIT
CCCHHHCCEEEEEECCCCCCCCCHHHHHHHCCCCCHHHCCEEEECCCCCCCEEEEECCCC
IETLPKVINAIEAAPRPRIIVALGSCGVGGGIWHDTYSTIGGVRSLKKILDEKGIQVDGI
HHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEECCE
YYVPGCPIRPEALLYAIALIKGLVSKKVKGEIKYAE
EECCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503 [H]