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The map label for this gene is hycE [H]

Identifier: 218884332

GI number: 218884332

Start: 970697

End: 972334

Strand: Direct

Name: hycE [H]

Synonym: DKAM_1021

Alternate gene names: 218884332

Gene position: 970697-972334 (Clockwise)

Preceding gene: 218884331

Following gene: 218884333

Centisome position: 71.1

GC content: 47.92

Gene sequence:

>1638_bases
ATGCATTCCCAGACGCTTCTAGATAGATACATCCAGCTAGCTGGGAAACTAGGCGGCAAGCATCTCGTGGAAGGAGACAT
GGTACACTTATACATACCCCGTGAAAACCTGGTTAAGGCTGCCTCCACATTGTTCAATGAATACAGACTAACACATAGGA
CGTGCGTCGCAATAGATGAGAGGCCATTAAACGGCTTCTTCACGCTGACACATATATTCACGGATGACTCATCGAGGCTA
TATGTATTAGTGAAAACATACCTTGAGGGAGATAACCCGGTTGCACCCAGCATAGTTGGCGAGGTACCAGCCGCGGACTG
GTGTGAGCTGGAAGCAATGGATTTACTAGGGATACAATTCGAGGGGAGGAGAAGAGAGAGACTTGTTCTACCACCATACT
GGCCCGATGGAGTATACCCATTAAGGAAGGAGTTCTCATATGATCAAAGGCTAAACCTCCATGCGGTATCGGAGAAAATA
GAGCAGGCTGGGGAAACCGGGGTACCAGTGCCTATAGGACCCTATCACCCGGCTCTCCACGAGCCAGAGTACTTCGAGCT
GTATGTTGAAGGAGAGCGGATCTTAGACGTTAAATACAGAGGATTCCATGTACATCGTGGAATAGAGAAACTAGCTGAAT
CCAGAATGACGTATCAACAGGTAAACTTTCTAGCAGAGAGGATATGCGGTATATGTGGCTTCGAGCACAGTACATGTTAC
GCCTTGGCAGTTGAAAAAGCTGCTAAACTAGATGTACCGGAGAGAGCGCAATTCATTAGAAGCATAGTCCTCGAAGTTGA
GAGGCTGCACAGCCACCTACTATGGCTCGGCGTCGCATTCCACACCCTTGGATACGATGCCGGCTTCATGCATATGTGGC
GTATAAGGGAAAAGGTAATGGAGCTGGCTGAAGCATTAACCGGGAGTAGGAAAACATATGGTATAAATATGGTTGGTGGG
GTACGGAGAGACATAAATGAGGAGAAGAGAAAGAAGACCATTGAAGTACTCAACGAGTTGGAGAAAGAGTTCAGTAAGAT
AATAGATGTCGCGATAAGCGTCCCACAAGTCAAGAAGAGGTTGACAGGTACAGGAGTACTACCAAAGGAGTGGGCCGTAA
AGCTAGGAGTCGAAGGCCCTGTTGCAAGGGCTTCAGGTGTAGACAGGGATGCCAGGAGGGATTTACCATACGCAGCCTAC
AAGCATGCATCGTTCAAGGTCCCGGTTTACACCGAGGGAGACAACATGGCTAGGGCACTAGTCAGAGTTGACGAGGTGAA
GGAGTCAATGTCGATAATAATACAACTACTAGACGCCATACCCAGGGGTCCCATAATGCTTGATAAGTATGAGATACCCG
AGGGAAGGATAGGGGTCCAGGTGGTTGAGGCCCCTAGGGGAGGAGATATACACTACGTGTTGACAGGTAAAGGGAAACCA
TATAGGTGGAGGGTCCGGGCTCCAACATATAATAACATCCCAGCGCTGAGGATAATGCTGAGGGATCAATCCCTAGCTGA
TGCACCGCTCACAATAGCCAGTATAGACCCATGCTTCTCATGTACTGATAGAATCATGGTTATAAGAGAGAATGGAAGAG
TGGAGAGAATTAGTTTTAACAGGGGTGTTCCACTATGA

Upstream 100 bases:

>100_bases
TGCTTGGGAGGAGCCGTATAGATCTCGCTCCAGGCACCTTATTTAAGGTATATATAGTCCTGTTAACGATATCTATAACA
GGGTTATTGGTGATTAAATA

Downstream 100 bases:

>100_bases
GTAAACTCCTTAAACCAGTTAAACTAGTAGTAATAGCTGGTGAAGCAGGGAGAGTAACAAGGAAGTATCCGTTTGAAGAA
CCACTTGTAACACCGGACTT

Product: NADH dehydrogenase (ubiquinone), 30 kDa subunit

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: FHL subunit 5; Hydrogenase-3 component E [H]

Number of amino acids: Translated: 545; Mature: 545

Protein sequence:

>545_residues
MHSQTLLDRYIQLAGKLGGKHLVEGDMVHLYIPRENLVKAASTLFNEYRLTHRTCVAIDERPLNGFFTLTHIFTDDSSRL
YVLVKTYLEGDNPVAPSIVGEVPAADWCELEAMDLLGIQFEGRRRERLVLPPYWPDGVYPLRKEFSYDQRLNLHAVSEKI
EQAGETGVPVPIGPYHPALHEPEYFELYVEGERILDVKYRGFHVHRGIEKLAESRMTYQQVNFLAERICGICGFEHSTCY
ALAVEKAAKLDVPERAQFIRSIVLEVERLHSHLLWLGVAFHTLGYDAGFMHMWRIREKVMELAEALTGSRKTYGINMVGG
VRRDINEEKRKKTIEVLNELEKEFSKIIDVAISVPQVKKRLTGTGVLPKEWAVKLGVEGPVARASGVDRDARRDLPYAAY
KHASFKVPVYTEGDNMARALVRVDEVKESMSIIIQLLDAIPRGPIMLDKYEIPEGRIGVQVVEAPRGGDIHYVLTGKGKP
YRWRVRAPTYNNIPALRIMLRDQSLADAPLTIASIDPCFSCTDRIMVIRENGRVERISFNRGVPL

Sequences:

>Translated_545_residues
MHSQTLLDRYIQLAGKLGGKHLVEGDMVHLYIPRENLVKAASTLFNEYRLTHRTCVAIDERPLNGFFTLTHIFTDDSSRL
YVLVKTYLEGDNPVAPSIVGEVPAADWCELEAMDLLGIQFEGRRRERLVLPPYWPDGVYPLRKEFSYDQRLNLHAVSEKI
EQAGETGVPVPIGPYHPALHEPEYFELYVEGERILDVKYRGFHVHRGIEKLAESRMTYQQVNFLAERICGICGFEHSTCY
ALAVEKAAKLDVPERAQFIRSIVLEVERLHSHLLWLGVAFHTLGYDAGFMHMWRIREKVMELAEALTGSRKTYGINMVGG
VRRDINEEKRKKTIEVLNELEKEFSKIIDVAISVPQVKKRLTGTGVLPKEWAVKLGVEGPVARASGVDRDARRDLPYAAY
KHASFKVPVYTEGDNMARALVRVDEVKESMSIIIQLLDAIPRGPIMLDKYEIPEGRIGVQVVEAPRGGDIHYVLTGKGKP
YRWRVRAPTYNNIPALRIMLRDQSLADAPLTIASIDPCFSCTDRIMVIRENGRVERISFNRGVPL
>Mature_545_residues
MHSQTLLDRYIQLAGKLGGKHLVEGDMVHLYIPRENLVKAASTLFNEYRLTHRTCVAIDERPLNGFFTLTHIFTDDSSRL
YVLVKTYLEGDNPVAPSIVGEVPAADWCELEAMDLLGIQFEGRRRERLVLPPYWPDGVYPLRKEFSYDQRLNLHAVSEKI
EQAGETGVPVPIGPYHPALHEPEYFELYVEGERILDVKYRGFHVHRGIEKLAESRMTYQQVNFLAERICGICGFEHSTCY
ALAVEKAAKLDVPERAQFIRSIVLEVERLHSHLLWLGVAFHTLGYDAGFMHMWRIREKVMELAEALTGSRKTYGINMVGG
VRRDINEEKRKKTIEVLNELEKEFSKIIDVAISVPQVKKRLTGTGVLPKEWAVKLGVEGPVARASGVDRDARRDLPYAAY
KHASFKVPVYTEGDNMARALVRVDEVKESMSIIIQLLDAIPRGPIMLDKYEIPEGRIGVQVVEAPRGGDIHYVLTGKGKP
YRWRVRAPTYNNIPALRIMLRDQSLADAPLTIASIDPCFSCTDRIMVIRENGRVERISFNRGVPL

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG3261

COG function: function code C; Ni,Fe-hydrogenase III large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 49 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI4758786, Length=386, Percent_Identity=29.0155440414508, Blast_Score=146, Evalue=5e-35,
Organism=Homo sapiens, GI260898743, Length=376, Percent_Identity=28.7234042553192, Blast_Score=140, Evalue=2e-33,
Organism=Escherichia coli, GI1789076, Length=478, Percent_Identity=45.397489539749, Blast_Score=422, Evalue=1e-119,
Organism=Escherichia coli, GI1788832, Length=478, Percent_Identity=41.8410041841004, Blast_Score=395, Evalue=1e-111,
Organism=Escherichia coli, GI145693162, Length=541, Percent_Identity=27.5415896487985, Blast_Score=185, Evalue=5e-48,
Organism=Caenorhabditis elegans, GI17568379, Length=383, Percent_Identity=29.5039164490862, Blast_Score=155, Evalue=5e-38,
Organism=Caenorhabditis elegans, GI17555284, Length=383, Percent_Identity=29.7650130548303, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24638644, Length=382, Percent_Identity=29.5811518324607, Blast_Score=170, Evalue=2e-42,
Organism=Drosophila melanogaster, GI221459469, Length=384, Percent_Identity=29.9479166666667, Blast_Score=162, Evalue=8e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001135
- InterPro:   IPR001268
- InterPro:   IPR014029
- InterPro:   IPR001501
- ProDom:   PD001581 [H]

Pfam domain/function: PF00329 Complex1_30kDa; PF00346 Complex1_49kDa; PF00374 NiFeSe_Hases [H]

EC number: NA

Molecular weight: Translated: 61914; Mature: 61914

Theoretical pI: Translated: 7.66; Mature: 7.66

Prosite motif: PS00535 COMPLEX1_49K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHSQTLLDRYIQLAGKLGGKHLVEGDMVHLYIPRENLVKAASTLFNEYRLTHRTCVAIDE
CCCHHHHHHHHHHHHHCCCCEEECCCEEEEEECHHHHHHHHHHHHHHHHHCCEEEEEECC
RPLNGFFTLTHIFTDDSSRLYVLVKTYLEGDNPVAPSIVGEVPAADWCELEAMDLLGIQF
CCCCCEEEEEEEEECCCCEEEEEEEEEECCCCCCCCHHHCCCCCCCCCCCCHHHEEEEEE
EGRRRERLVLPPYWPDGVYPLRKEFSYDQRLNLHAVSEKIEQAGETGVPVPIGPYHPALH
CCCCCCEEEECCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCC
EPEYFELYVEGERILDVKYRGFHVHRGIEKLAESRMTYQQVNFLAERICGICGFEHSTCY
CCCEEEEEECCCEEEEEEECCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
ALAVEKAAKLDVPERAQFIRSIVLEVERLHSHLLWLGVAFHTLGYDAGFMHMWRIREKVM
EEEHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
ELAEALTGSRKTYGINMVGGVRRDINEEKRKKTIEVLNELEKEFSKIIDVAISVPQVKKR
HHHHHHCCCCCEEEEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
LTGTGVLPKEWAVKLGVEGPVARASGVDRDARRDLPYAAYKHASFKVPVYTEGDNMARAL
HCCCCCCCHHHHEEECCCCCCHHHCCCCCHHHCCCCHHHHCCCEEEEEEEECCCHHHHHH
VRVDEVKESMSIIIQLLDAIPRGPIMLDKYEIPEGRIGVQVVEAPRGGDIHYVLTGKGKP
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEEEECCCCCCEEEEEECCCCC
YRWRVRAPTYNNIPALRIMLRDQSLADAPLTIASIDPCFSCTDRIMVIRENGRVERISFN
EEEEEECCCCCCCCEEEEEEECCCCCCCCEEEEECCCCCCCCCEEEEEECCCCEEEEECC
RGVPL
CCCCC
>Mature Secondary Structure
MHSQTLLDRYIQLAGKLGGKHLVEGDMVHLYIPRENLVKAASTLFNEYRLTHRTCVAIDE
CCCHHHHHHHHHHHHHCCCCEEECCCEEEEEECHHHHHHHHHHHHHHHHHCCEEEEEECC
RPLNGFFTLTHIFTDDSSRLYVLVKTYLEGDNPVAPSIVGEVPAADWCELEAMDLLGIQF
CCCCCEEEEEEEEECCCCEEEEEEEEEECCCCCCCCHHHCCCCCCCCCCCCHHHEEEEEE
EGRRRERLVLPPYWPDGVYPLRKEFSYDQRLNLHAVSEKIEQAGETGVPVPIGPYHPALH
CCCCCCEEEECCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCC
EPEYFELYVEGERILDVKYRGFHVHRGIEKLAESRMTYQQVNFLAERICGICGFEHSTCY
CCCEEEEEECCCEEEEEEECCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
ALAVEKAAKLDVPERAQFIRSIVLEVERLHSHLLWLGVAFHTLGYDAGFMHMWRIREKVM
EEEHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
ELAEALTGSRKTYGINMVGGVRRDINEEKRKKTIEVLNELEKEFSKIIDVAISVPQVKKR
HHHHHHCCCCCEEEEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
LTGTGVLPKEWAVKLGVEGPVARASGVDRDARRDLPYAAYKHASFKVPVYTEGDNMARAL
HCCCCCCCHHHHEEECCCCCCHHHCCCCCHHHCCCCHHHHCCCEEEEEEEECCCHHHHHH
VRVDEVKESMSIIIQLLDAIPRGPIMLDKYEIPEGRIGVQVVEAPRGGDIHYVLTGKGKP
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEEEECCCCCCEEEEEECCCCC
YRWRVRAPTYNNIPALRIMLRDQSLADAPLTIASIDPCFSCTDRIMVIRENGRVERISFN
EEEEEECCCCCCCCEEEEEEECCCCCCCCEEEEECCCCCCCCCEEEEEECCCCEEEEECC
RGVPL
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503; 8125094 [H]