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The map label for this gene is birA [H]

Identifier: 218884068

GI number: 218884068

Start: 730285

End: 731190

Strand: Reverse

Name: birA [H]

Synonym: DKAM_0757

Alternate gene names: 218884068

Gene position: 731190-730285 (Counterclockwise)

Preceding gene: 218884069

Following gene: 218884065

Centisome position: 53.56

GC content: 45.14

Gene sequence:

>906_bases
ATGAGTAGCGAGCTCGCATTAAAGCTTGAACTGGTGAGAATTCTCTCCGAGCACGACGAGATACCTATCTCCGAAGTGGT
GAGAGAGATAGGTGTACAACAGGATGAATTAATCAGCATGATTAACGAGTTATCCAGGTATTACTTAATGGAGACCGCGA
GATCCAGGGTTTCATGGAGCCCGGGCGATAATCCACGTAGATTAAAACCATGGGGTTGGAACTACCTATATAGAATTATA
ACAGGATCCACGATGACTTCAGCGAAACAGATGCCACCATGGAGTATAGTTGTATCAGAGATGCAGACACAGGGTAGAGG
AAGACATGGAAAGACATGGATAGGGAATCTCGGAGGATTATGGATCACGTTCAAGATTAATACCACACCCCAGGTAACAC
AGGTTCTTTCAATAGCGATACCAGTCCTCCTAGTCAGGATGCTAAGAGATAAACTCGGTATAAATGTTGAAATCAAGTGG
CCTAACGACATAATATTTGGGGATAAAAAGATCGCTGGGATACTCCTGGAGGGCGAGTACAAGGAGTCAGTAATGGTCAC
CTATATCGGGATAGGCATAAACGTTAATAATGACCCCCCGCTTGAAGTGGCTGAGAGCATTAAGAACATAGTGGGACACC
TGATACCGCGTAACAGGATCCTCTCCTATCTCTCCGGGTGGATTGGCAGGTTGGATAGATTAGCCGGTAGGCCAGATGAG
TTGAGGATGGAGTATATAGAGCATCTCTCCACACTAGGTAGAAAAGTCATAGCTATGACAAGCAGTGGTGAAATACAGGG
GATCGCAAAGGATGTGACAGAGTATGGAGAGCTGATCATTGAAAACGAATCGGGCAGCCATAGATTATCAAGTGGTGAAA
TACTTAGGTTAAGGCATGCCGAGTAA

Upstream 100 bases:

>100_bases
CCTCTATGAATTTTTGAGGAAATACTACCCGGATCTCCCTAACATGCTTAACATTGATAAAGTCGTTGAGAAGATTAAAC
TATTAGAGGAGGAGAAGTAA

Downstream 100 bases:

>100_bases
CCACCTTACCTCCCAGCCACATTAACCCCGTGGATCCATAGGCTTGGGGAGCCAACCCCGTTATTCACAGCTAGATCTCC
TCCAACACCTGCAAGACCGT

Product: biotin--acetyl-CoA-carboxylase ligase

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 301; Mature: 300

Protein sequence:

>301_residues
MSSELALKLELVRILSEHDEIPISEVVREIGVQQDELISMINELSRYYLMETARSRVSWSPGDNPRRLKPWGWNYLYRII
TGSTMTSAKQMPPWSIVVSEMQTQGRGRHGKTWIGNLGGLWITFKINTTPQVTQVLSIAIPVLLVRMLRDKLGINVEIKW
PNDIIFGDKKIAGILLEGEYKESVMVTYIGIGINVNNDPPLEVAESIKNIVGHLIPRNRILSYLSGWIGRLDRLAGRPDE
LRMEYIEHLSTLGRKVIAMTSSGEIQGIAKDVTEYGELIIENESGSHRLSSGEILRLRHAE

Sequences:

>Translated_301_residues
MSSELALKLELVRILSEHDEIPISEVVREIGVQQDELISMINELSRYYLMETARSRVSWSPGDNPRRLKPWGWNYLYRII
TGSTMTSAKQMPPWSIVVSEMQTQGRGRHGKTWIGNLGGLWITFKINTTPQVTQVLSIAIPVLLVRMLRDKLGINVEIKW
PNDIIFGDKKIAGILLEGEYKESVMVTYIGIGINVNNDPPLEVAESIKNIVGHLIPRNRILSYLSGWIGRLDRLAGRPDE
LRMEYIEHLSTLGRKVIAMTSSGEIQGIAKDVTEYGELIIENESGSHRLSSGEILRLRHAE
>Mature_300_residues
SSELALKLELVRILSEHDEIPISEVVREIGVQQDELISMINELSRYYLMETARSRVSWSPGDNPRRLKPWGWNYLYRIIT
GSTMTSAKQMPPWSIVVSEMQTQGRGRHGKTWIGNLGGLWITFKINTTPQVTQVLSIAIPVLLVRMLRDKLGINVEIKWP
NDIIFGDKKIAGILLEGEYKESVMVTYIGIGINVNNDPPLEVAESIKNIVGHLIPRNRILSYLSGWIGRLDRLAGRPDEL
RMEYIEHLSTLGRKVIAMTSSGEIQGIAKDVTEYGELIIENESGSHRLSSGEILRLRHAE

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Homo sapiens, GI46255045, Length=133, Percent_Identity=31.5789473684211, Blast_Score=65, Evalue=5e-11,
Organism=Escherichia coli, GI1790408, Length=222, Percent_Identity=29.2792792792793, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 34039; Mature: 33908

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSELALKLELVRILSEHDEIPISEVVREIGVQQDELISMINELSRYYLMETARSRVSWS
CCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
PGDNPRRLKPWGWNYLYRIITGSTMTSAKQMPPWSIVVSEMQTQGRGRHGKTWIGNLGGL
CCCCCCCCCCCCHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHCCCCCCCCCEEECCCCE
WITFKINTTPQVTQVLSIAIPVLLVRMLRDKLGINVEIKWPNDIIFGDKKIAGILLEGEY
EEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEECCCEEEEEEEECCC
KESVMVTYIGIGINVNNDPPLEVAESIKNIVGHLIPRNRILSYLSGWIGRLDRLAGRPDE
CCCEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHH
LRMEYIEHLSTLGRKVIAMTSSGEIQGIAKDVTEYGELIIENESGSHRLSSGEILRLRHA
HHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHCCEEEECCCCCCCCCCCCEEEEECC
E
C
>Mature Secondary Structure 
SSELALKLELVRILSEHDEIPISEVVREIGVQQDELISMINELSRYYLMETARSRVSWS
CCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
PGDNPRRLKPWGWNYLYRIITGSTMTSAKQMPPWSIVVSEMQTQGRGRHGKTWIGNLGGL
CCCCCCCCCCCCHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHCCCCCCCCCEEECCCCE
WITFKINTTPQVTQVLSIAIPVLLVRMLRDKLGINVEIKWPNDIIFGDKKIAGILLEGEY
EEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEECCCEEEEEEEECCC
KESVMVTYIGIGINVNNDPPLEVAESIKNIVGHLIPRNRILSYLSGWIGRLDRLAGRPDE
CCCEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHH
LRMEYIEHLSTLGRKVIAMTSSGEIQGIAKDVTEYGELIIENESGSHRLSSGEILRLRHA
HHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHCCEEEECCCCCCCCCCCCEEEEECC
E
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7730294 [H]