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The map label for this gene is ligT [C]

Identifier: 218883633

GI number: 218883633

Start: 296736

End: 297314

Strand: Reverse

Name: ligT [C]

Synonym: DKAM_0322

Alternate gene names: 218883633

Gene position: 297314-296736 (Counterclockwise)

Preceding gene: 218883639

Following gene: 218883632

Centisome position: 21.78

GC content: 47.5

Gene sequence:

>579_bases
GTGGTGTCATTGAGCGGTGAGATCATAAGGTGCTTCATAGCCATCGAGCTGAAGAATCAGGAGGTCCTGAGAAGACTCAT
CGAGATAAGGGATAAGATAACTGCCACCGGGGTGGATCTAAAGCCTGTTGAAGACGAGAATATTCATTTAACCCTGAGAT
TCATTGGTGAAGTACCACGAAGCGTCGTTGACGAGGTGTGCGGCTTCATAAACACGATCTCATACAAGGGGTTCAAAATA
CATGTTAAGAACATAGGGGGCTTTCCAAGTCTTGAGAGGCCAAGGGTGATCTGGGCTGGGATTGAGGAAGGGAGTGATGA
GTTATTGGAATTATACAGGATCGTGGAGGCTGGGTTAAGGAGGATCGGTATTAAACCTGAGAGGGAGGAGTTCACTCCAC
ATATAACCCTAGCCAGGGTCAAGGGGTATAGGGGTATTGAGAAACTCGTGAAGACGCTTATGGAGCTAAGGGGGACGGAC
TTCGGGTATTCACCGGTGGAGGAGGTAATAGTTAAGAAAAGCGTCTTAACGCCGCGTGGACCAATATATAGCAATATATG
CGTGAAAAAGCTTGCCTAG

Upstream 100 bases:

>100_bases
GGCATCCCCACGATTAGTATGAATAACATGAATACTACACCAGCTGGTTTAGGATAGTTAATATATTTTGGAGGCGTTTT
AATGTTTCATAGAGTTAGGG

Downstream 100 bases:

>100_bases
CAGTGGTGGATTATTGAAATGACTGCCAGCGAGATCGAGGAGGCTGTGCTTAAAACAATCAAGCCCAGCAGGGAGGAGTA
CGAGCATGTACTAGGTGTAT

Product: 2'-5' RNA ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 192; Mature: 192

Protein sequence:

>192_residues
MVSLSGEIIRCFIAIELKNQEVLRRLIEIRDKITATGVDLKPVEDENIHLTLRFIGEVPRSVVDEVCGFINTISYKGFKI
HVKNIGGFPSLERPRVIWAGIEEGSDELLELYRIVEAGLRRIGIKPEREEFTPHITLARVKGYRGIEKLVKTLMELRGTD
FGYSPVEEVIVKKSVLTPRGPIYSNICVKKLA

Sequences:

>Translated_192_residues
MVSLSGEIIRCFIAIELKNQEVLRRLIEIRDKITATGVDLKPVEDENIHLTLRFIGEVPRSVVDEVCGFINTISYKGFKI
HVKNIGGFPSLERPRVIWAGIEEGSDELLELYRIVEAGLRRIGIKPEREEFTPHITLARVKGYRGIEKLVKTLMELRGTD
FGYSPVEEVIVKKSVLTPRGPIYSNICVKKLA
>Mature_192_residues
MVSLSGEIIRCFIAIELKNQEVLRRLIEIRDKITATGVDLKPVEDENIHLTLRFIGEVPRSVVDEVCGFINTISYKGFKI
HVKNIGGFPSLERPRVIWAGIEEGSDELLELYRIVEAGLRRIGIKPEREEFTPHITLARVKGYRGIEKLVKTLMELRGTD
FGYSPVEEVIVKKSVLTPRGPIYSNICVKKLA

Specific function: Specifically Ligates tRNA Half-Molecules Containing Nucleoside Base Modifications And Shows A Preference Among Different tRNA Species. Acts In The Absence Of ATP To Form A 2'-5' Phosphodiester Linkage. [C]

COG id: COG1514

COG function: function code J; 2'-5' RNA ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UPF0097 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004175
- InterPro:   IPR014051
- InterPro:   IPR009097 [H]

Pfam domain/function: PF02834 2_5_RNA_ligase [H]

EC number: 6.5.1.- [C]

Molecular weight: Translated: 21759; Mature: 21759

Theoretical pI: Translated: 8.81; Mature: 8.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSLSGEIIRCFIAIELKNQEVLRRLIEIRDKITATGVDLKPVEDENIHLTLRFIGEVPR
CCCCCHHEEEEEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEHHHHHCHH
SVVDEVCGFINTISYKGFKIHVKNIGGFPSLERPRVIWAGIEEGSDELLELYRIVEAGLR
HHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHH
RIGIKPEREEFTPHITLARVKGYRGIEKLVKTLMELRGTDFGYSPVEEVIVKKSVLTPRG
HCCCCCCHHHCCCCEEEEHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCC
PIYSNICVKKLA
CCHHHHHHHHCC
>Mature Secondary Structure
MVSLSGEIIRCFIAIELKNQEVLRRLIEIRDKITATGVDLKPVEDENIHLTLRFIGEVPR
CCCCCHHEEEEEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEHHHHHCHH
SVVDEVCGFINTISYKGFKIHVKNIGGFPSLERPRVIWAGIEEGSDELLELYRIVEAGLR
HHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHH
RIGIKPEREEFTPHITLARVKGYRGIEKLVKTLMELRGTDFGYSPVEEVIVKKSVLTPRG
HCCCCCCHHHCCCCEEEEHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCC
PIYSNICVKKLA
CCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10382966 [H]