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The map label for this gene is cca [H]

Identifier: 218883632

GI number: 218883632

Start: 295317

End: 296717

Strand: Reverse

Name: cca [H]

Synonym: DKAM_0321

Alternate gene names: 218883632

Gene position: 296717-295317 (Counterclockwise)

Preceding gene: 218883633

Following gene: 218883631

Centisome position: 21.73

GC content: 46.82

Gene sequence:

>1401_bases
ATGACTGCCAGCGAGATCGAGGAGGCTGTGCTTAAAACAATCAAGCCCAGCAGGGAGGAGTACGAGCATGTACTAGGTGT
ATACCGTGTGATCGCCGATGCTATAAACACCGTGCTGAGAAAGCATGGTGTCGAAGCCGAGGTAACTCTTCAGGGAAGCA
TAGCGCATGATACATGGCTATCTGGAGACAGGGATATGGATGTATTCGTCCTGTACCCTGAATCATGGAGTATCGAGGAG
TTGAAGAGTAAAGGGTTTAAGCTAGTGGTTGAAGCCGCGGAGAAACTTGGGATATATGAGTTAAGGTATGCTGAACACCC
CTATGTAAGGGTCAGGATCAGCGATGTTGAAGCAGATATCGTGCCAGCGTTCAACATATCAGACCCGGCACATATTAGAA
CAGCTGTGGATAGGACGCCGTTCCACACGAGGTACATTAGGGAGAGGCTCACGGATGAGATGAGAGACCAGGTTAGATTA
TTAAAGAAGTTTATGAAAGGTATTGGGGTATATGGTGCCGAGGTCAAGACAAAGGGGTTCAGCGGCTATGCTGTGGAAGT
ACTTATAGCCAGGTATAATTCCATGAGGAGGGTTCTCGAGGAGGCATCTAAATGGAATCCCCCGGTTTACATTGACTCGA
TAAACGTTGGCGAATCTTTCTGGAAGGGGTTTAGGAAAAAGTATCCTGACTCAGTAATATATATGCCGGACCCGGTGGAT
CCCATGAGGAATGTAACGGCAAATGTATCGATGAAAAGCCTTGCAATATTCAGCCTGGCATCCAGGTGTTATCTCACGCA
TCCATCACTAGTATTCTACGGCTTAGAGTACGAGGGTCCATCTATAGATGATGTGAAGAAAAAGCTCGCGGATAGATGCA
TAGTCCTAGTTGAGTACATGCTGGAGGAGAGACTGCCCCCTGATGTTGTATGGGGCGAGTTGAACAGGGTTAGGGATACC
ATGGTTAAGCTCCTCGAGAACTTTGATTTCCACGTAATCGATTCATCATCGTGGACTGACGAGGACAGGTACTGTGCTAT
ATTATTGGAGCTTGATTCCTGTAGTCTCCCAGTATATAAACACTATAAGGGTCCCTCTGTGAAGCACTGGGAGAGATCAC
ATAGCTTCATATCGAAGCATCTTGGTAGAGGGATCGGTGTATGGGTTGATGAGAAGGGCTCCCTGAACGCGTTATCCCTT
AGAAGATACATTAATGTAAAGCAAATACTGGAGGAGGGAAGCCGAGGGTACTCGGTTGCACCACACTTTAGGGATATAAA
ACCATCGGTGAAGGCCTTAAGCGAGGAGGTGATAGCAGAGTTATCGGGAAGAGGGGCTTTACGATGGATTTCAGAATTTA
TATTGAAGACCCCTATATGGATGGTGAAGTGCATTTCCTAG

Upstream 100 bases:

>100_bases
GGAGGAGGTAATAGTTAAGAAAAGCGTCTTAACGCCGCGTGGACCAATATATAGCAATATATGCGTGAAAAAGCTTGCCT
AGCAGTGGTGGATTATTGAA

Downstream 100 bases:

>100_bases
TCAACTACCCCCGGGATGAATACATATATAGAGTACTATGCTTCCCGGCATGCAATAGAAGAGATTTAATAGTTGAGAGA
GCCCTGGAGCTGATTAAGGA

Product: tRNA CCA-pyrophosphorylase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MTASEIEEAVLKTIKPSREEYEHVLGVYRVIADAINTVLRKHGVEAEVTLQGSIAHDTWLSGDRDMDVFVLYPESWSIEE
LKSKGFKLVVEAAEKLGIYELRYAEHPYVRVRISDVEADIVPAFNISDPAHIRTAVDRTPFHTRYIRERLTDEMRDQVRL
LKKFMKGIGVYGAEVKTKGFSGYAVEVLIARYNSMRRVLEEASKWNPPVYIDSINVGESFWKGFRKKYPDSVIYMPDPVD
PMRNVTANVSMKSLAIFSLASRCYLTHPSLVFYGLEYEGPSIDDVKKKLADRCIVLVEYMLEERLPPDVVWGELNRVRDT
MVKLLENFDFHVIDSSSWTDEDRYCAILLELDSCSLPVYKHYKGPSVKHWERSHSFISKHLGRGIGVWVDEKGSLNALSL
RRYINVKQILEEGSRGYSVAPHFRDIKPSVKALSEEVIAELSGRGALRWISEFILKTPIWMVKCIS

Sequences:

>Translated_466_residues
MTASEIEEAVLKTIKPSREEYEHVLGVYRVIADAINTVLRKHGVEAEVTLQGSIAHDTWLSGDRDMDVFVLYPESWSIEE
LKSKGFKLVVEAAEKLGIYELRYAEHPYVRVRISDVEADIVPAFNISDPAHIRTAVDRTPFHTRYIRERLTDEMRDQVRL
LKKFMKGIGVYGAEVKTKGFSGYAVEVLIARYNSMRRVLEEASKWNPPVYIDSINVGESFWKGFRKKYPDSVIYMPDPVD
PMRNVTANVSMKSLAIFSLASRCYLTHPSLVFYGLEYEGPSIDDVKKKLADRCIVLVEYMLEERLPPDVVWGELNRVRDT
MVKLLENFDFHVIDSSSWTDEDRYCAILLELDSCSLPVYKHYKGPSVKHWERSHSFISKHLGRGIGVWVDEKGSLNALSL
RRYINVKQILEEGSRGYSVAPHFRDIKPSVKALSEEVIAELSGRGALRWISEFILKTPIWMVKCIS
>Mature_465_residues
TASEIEEAVLKTIKPSREEYEHVLGVYRVIADAINTVLRKHGVEAEVTLQGSIAHDTWLSGDRDMDVFVLYPESWSIEEL
KSKGFKLVVEAAEKLGIYELRYAEHPYVRVRISDVEADIVPAFNISDPAHIRTAVDRTPFHTRYIRERLTDEMRDQVRLL
KKFMKGIGVYGAEVKTKGFSGYAVEVLIARYNSMRRVLEEASKWNPPVYIDSINVGESFWKGFRKKYPDSVIYMPDPVDP
MRNVTANVSMKSLAIFSLASRCYLTHPSLVFYGLEYEGPSIDDVKKKLADRCIVLVEYMLEERLPPDVVWGELNRVRDTM
VKLLENFDFHVIDSSSWTDEDRYCAILLELDSCSLPVYKHYKGPSVKHWERSHSFISKHLGRGIGVWVDEKGSLNALSLR
RYINVKQILEEGSRGYSVAPHFRDIKPSVKALSEEVIAELSGRGALRWISEFILKTPIWMVKCIS

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG1746

COG function: function code J; tRNA nucleotidyltransferase (CCA-adding enzyme)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Archaeal CCA-adding enzyme subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006116
- InterPro:   IPR008229
- InterPro:   IPR002934
- InterPro:   IPR011068
- InterPro:   IPR015329 [H]

Pfam domain/function: PF01909 NTP_transf_2; PF09249 tRNA_NucTransf2 [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 53464; Mature: 53333

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASEIEEAVLKTIKPSREEYEHVLGVYRVIADAINTVLRKHGVEAEVTLQGSIAHDTWL
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCC
SGDRDMDVFVLYPESWSIEELKSKGFKLVVEAAEKLGIYELRYAEHPYVRVRISDVEADI
CCCCCCEEEEECCCCCCHHHHHHCCCEEHHHHHHHCCCEEEEECCCCEEEEEEECCCCCE
VPAFNISDPAHIRTAVDRTPFHTRYIRERLTDEMRDQVRLLKKFMKGIGVYGAEVKTKGF
EECCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCC
SGYAVEVLIARYNSMRRVLEEASKWNPPVYIDSINVGESFWKGFRKKYPDSVIYMPDPVD
CCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCCEEECCCCCC
PMRNVTANVSMKSLAIFSLASRCYLTHPSLVFYGLEYEGPSIDDVKKKLADRCIVLVEYM
HHHCCCCCCCHHHHHHHHHHHHHEEECCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
LEERLPPDVVWGELNRVRDTMVKLLENFDFHVIDSSSWTDEDRYCAILLELDSCSLPVYK
HHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCEEEEEEEECCCCCCHHH
HYKGPSVKHWERSHSFISKHLGRGIGVWVDEKGSLNALSLRRYINVKQILEEGSRGYSVA
CCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
PHFRDIKPSVKALSEEVIAELSGRGALRWISEFILKTPIWMVKCIS
CCHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHEEECC
>Mature Secondary Structure 
TASEIEEAVLKTIKPSREEYEHVLGVYRVIADAINTVLRKHGVEAEVTLQGSIAHDTWL
CHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCC
SGDRDMDVFVLYPESWSIEELKSKGFKLVVEAAEKLGIYELRYAEHPYVRVRISDVEADI
CCCCCCEEEEECCCCCCHHHHHHCCCEEHHHHHHHCCCEEEEECCCCEEEEEEECCCCCE
VPAFNISDPAHIRTAVDRTPFHTRYIRERLTDEMRDQVRLLKKFMKGIGVYGAEVKTKGF
EECCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCC
SGYAVEVLIARYNSMRRVLEEASKWNPPVYIDSINVGESFWKGFRKKYPDSVIYMPDPVD
CCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCCEEECCCCCC
PMRNVTANVSMKSLAIFSLASRCYLTHPSLVFYGLEYEGPSIDDVKKKLADRCIVLVEYM
HHHCCCCCCCHHHHHHHHHHHHHEEECCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
LEERLPPDVVWGELNRVRDTMVKLLENFDFHVIDSSSWTDEDRYCAILLELDSCSLPVYK
HHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCEEEEEEEECCCCCCHHH
HYKGPSVKHWERSHSFISKHLGRGIGVWVDEKGSLNALSLRRYINVKQILEEGSRGYSVA
CCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
PHFRDIKPSVKALSEEVIAELSGRGALRWISEFILKTPIWMVKCIS
CCHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11572479 [H]