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The map label for this gene is glyA [H]

Identifier: 218883611

GI number: 218883611

Start: 279917

End: 281263

Strand: Direct

Name: glyA [H]

Synonym: DKAM_0300

Alternate gene names: 218883611

Gene position: 279917-281263 (Clockwise)

Preceding gene: 218883610

Following gene: 218883612

Centisome position: 20.5

GC content: 49.15

Gene sequence:

>1347_bases
TTGGTATATGAATCGGGTGAAGCCCTAGGCATAGATGCTCTTAGAGAAATGTATCCAGACCTCAACCAGGTATTAAGCAT
CACCATAAACCACACGGTATGGAGGAAGCGGCAAACAATAAACATGATTGCAAGCGAGAATGTTATGTCACCCCTTGCAA
TGCTCGTGTACCTGAACGACATGATGCATAGGTATGCAGAAGGGAAGCCGTATAAGAGGTTCTACCAGGGGCTCATATAC
GTTGATGAGTTAGAGGTTAAAGCCCAGGAGTTAATGGGTGATCTCCTAGGCACTAAATACGTGGATTTAAGACCTATAAG
CGGTACGACAGCGAATGCGACGGCCTTTAGAACATTCACGAAGCCAGGTGATAAAGCAGTAGTTGCCCCTGTTCAAGCCG
GTGCACACGTAAGTCACACCAGGTATGGGACCCTTGGGGCGCTGGGCATAGAGCAGATTGAGATGCCGTTCGACATAGAA
GAGTGGAATATAGATGTTGATAAAGCCAGGAAGCTCATCGAAGAAGTTAAGCCGAAGATAGTGACTCTGGGCGGCTCACT
CTACATATTCCCGCATCCAACCAAGGAGATCGCTGAGGCAGCGCACAGTGTGGGGGCTAAGGTAATACATGATGTGGCAC
ATGTCTTGGGCTTAATAGTTGGAGGCGTATGGGAGAACCCGTTGAAGCTTGGGGCAGATGTAATCACCTCGTCAACGCAT
AAGACATTCCCAGGTCCACAAGGCGGAGTATTCGCTACCTCAAACGAGGAGGACTATAAGGAAATGGGTAAAGTCGTCTT
CCCAATGTTCGTCTCCAACCACCATCTCCATAGATTAGCTGCAATGGCTGTTACAGCAATAGAGATGAAGCTCTGGGGCT
CCGAGTACGCTAGGCAGGTGGTTAGAAATGCTAAAGCACTAGCGGAGGCCCTTGCAAGCGAGGGCTTCAAGGTTGTGATG
GAGAGCAAGGGTTATACCACGAGCCACCAGGTGGTAGTAGACGTTGCAGAGCTCGGCAGAGGCACCAAGGTAGCTAAGCT
ACTAGAGGACGCCCACATCATAGTCAACAAGAACATGCTACCATGGGATAGGCCGGAGGATGTCAAAGATCCAAGCGGGC
TGAGACTTGGAACCCAGGAGTTAACCAGGTGGGGTATGAAGGAGGGCGAGATGAAGGAGATAGCTAGGCTCATGAAGATG
GTTGTGATAGATAAGAGGAGCCCAGCTGAAGTGAAGGAGAAAGTCATGGAGTTCAAGAAGGAGTTCATGGAGATACACTA
CGGCTTCAAGCTGACGAGGGAGGATGAGGTAAAACTATTAAAGATAATGCTTCACGCGGAAACTTAA

Upstream 100 bases:

>100_bases
GAAGCGATACCCTGCTAAAATTGTTGACTTTCCATTCATAAAGAAATGAGTATTTATAGGATCGTTAAGACATAGTAAGG
TAAATGTAGGTGGATTGAAA

Downstream 100 bases:

>100_bases
TATTATCCGGCCGCATAGCTATACATGGTGGGATACATGAGTGAAGTCATTGTAGAGGTTAAGGGTAAGAAATACGTTGT
TAAAACGGATAGAAGGTACA

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase [H]

Number of amino acids: Translated: 448; Mature: 448

Protein sequence:

>448_residues
MVYESGEALGIDALREMYPDLNQVLSITINHTVWRKRQTINMIASENVMSPLAMLVYLNDMMHRYAEGKPYKRFYQGLIY
VDELEVKAQELMGDLLGTKYVDLRPISGTTANATAFRTFTKPGDKAVVAPVQAGAHVSHTRYGTLGALGIEQIEMPFDIE
EWNIDVDKARKLIEEVKPKIVTLGGSLYIFPHPTKEIAEAAHSVGAKVIHDVAHVLGLIVGGVWENPLKLGADVITSSTH
KTFPGPQGGVFATSNEEDYKEMGKVVFPMFVSNHHLHRLAAMAVTAIEMKLWGSEYARQVVRNAKALAEALASEGFKVVM
ESKGYTTSHQVVVDVAELGRGTKVAKLLEDAHIIVNKNMLPWDRPEDVKDPSGLRLGTQELTRWGMKEGEMKEIARLMKM
VVIDKRSPAEVKEKVMEFKKEFMEIHYGFKLTREDEVKLLKIMLHAET

Sequences:

>Translated_448_residues
MVYESGEALGIDALREMYPDLNQVLSITINHTVWRKRQTINMIASENVMSPLAMLVYLNDMMHRYAEGKPYKRFYQGLIY
VDELEVKAQELMGDLLGTKYVDLRPISGTTANATAFRTFTKPGDKAVVAPVQAGAHVSHTRYGTLGALGIEQIEMPFDIE
EWNIDVDKARKLIEEVKPKIVTLGGSLYIFPHPTKEIAEAAHSVGAKVIHDVAHVLGLIVGGVWENPLKLGADVITSSTH
KTFPGPQGGVFATSNEEDYKEMGKVVFPMFVSNHHLHRLAAMAVTAIEMKLWGSEYARQVVRNAKALAEALASEGFKVVM
ESKGYTTSHQVVVDVAELGRGTKVAKLLEDAHIIVNKNMLPWDRPEDVKDPSGLRLGTQELTRWGMKEGEMKEIARLMKM
VVIDKRSPAEVKEKVMEFKKEFMEIHYGFKLTREDEVKLLKIMLHAET
>Mature_448_residues
MVYESGEALGIDALREMYPDLNQVLSITINHTVWRKRQTINMIASENVMSPLAMLVYLNDMMHRYAEGKPYKRFYQGLIY
VDELEVKAQELMGDLLGTKYVDLRPISGTTANATAFRTFTKPGDKAVVAPVQAGAHVSHTRYGTLGALGIEQIEMPFDIE
EWNIDVDKARKLIEEVKPKIVTLGGSLYIFPHPTKEIAEAAHSVGAKVIHDVAHVLGLIVGGVWENPLKLGADVITSSTH
KTFPGPQGGVFATSNEEDYKEMGKVVFPMFVSNHHLHRLAAMAVTAIEMKLWGSEYARQVVRNAKALAEALASEGFKVVM
ESKGYTTSHQVVVDVAELGRGTKVAKLLEDAHIIVNKNMLPWDRPEDVKDPSGLRLGTQELTRWGMKEGEMKEIARLMKM
VVIDKRSPAEVKEKVMEFKKEFMEIHYGFKLTREDEVKLLKIMLHAET

Specific function: Interconversion of serine and glycine [H]

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family [H]

Homologues:

Organism=Homo sapiens, GI19923315, Length=420, Percent_Identity=28.5714285714286, Blast_Score=148, Evalue=8e-36,
Organism=Homo sapiens, GI261862352, Length=420, Percent_Identity=28.5714285714286, Blast_Score=148, Evalue=9e-36,
Organism=Homo sapiens, GI261862350, Length=420, Percent_Identity=28.5714285714286, Blast_Score=148, Evalue=9e-36,
Organism=Homo sapiens, GI261862348, Length=420, Percent_Identity=28.5714285714286, Blast_Score=148, Evalue=9e-36,
Organism=Homo sapiens, GI261862346, Length=415, Percent_Identity=28.433734939759, Blast_Score=145, Evalue=5e-35,
Organism=Homo sapiens, GI22547186, Length=420, Percent_Identity=28.5714285714286, Blast_Score=139, Evalue=7e-33,
Organism=Homo sapiens, GI22547189, Length=406, Percent_Identity=28.0788177339901, Blast_Score=127, Evalue=2e-29,
Organism=Escherichia coli, GI1788902, Length=396, Percent_Identity=34.8484848484849, Blast_Score=176, Evalue=4e-45,
Organism=Caenorhabditis elegans, GI25144732, Length=454, Percent_Identity=28.8546255506608, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI25144729, Length=454, Percent_Identity=28.8546255506608, Blast_Score=160, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6319739, Length=419, Percent_Identity=27.6849642004773, Blast_Score=150, Evalue=3e-37,
Organism=Saccharomyces cerevisiae, GI6323087, Length=412, Percent_Identity=29.6116504854369, Blast_Score=150, Evalue=4e-37,
Organism=Drosophila melanogaster, GI24640005, Length=385, Percent_Identity=31.9480519480519, Blast_Score=171, Evalue=9e-43,
Organism=Drosophila melanogaster, GI221329721, Length=384, Percent_Identity=31.25, Blast_Score=171, Evalue=9e-43,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798 [H]

Pfam domain/function: PF00464 SHMT [H]

EC number: =2.1.2.1 [H]

Molecular weight: Translated: 50159; Mature: 50159

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVYESGEALGIDALREMYPDLNQVLSITINHTVWRKRQTINMIASENVMSPLAMLVYLND
CCCCCCCCCCHHHHHHHCCCHHHHHEEHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MMHRYAEGKPYKRFYQGLIYVDELEVKAQELMGDLLGTKYVDLRPISGTTANATAFRTFT
HHHHHHCCCHHHHHHCCCEEEEHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCEEEEEC
KPGDKAVVAPVQAGAHVSHTRYGTLGALGIEQIEMPFDIEEWNIDVDKARKLIEEVKPKI
CCCCCEEEEECCCCCCCCCCCCCCHHHCCHHHCCCCCCHHHCCCCHHHHHHHHHHCCCCE
VTLGGSLYIFPHPTKEIAEAAHSVGAKVIHDVAHVLGLIVGGVWENPLKLGADVITSSTH
EEECCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCHHHHCCCCC
KTFPGPQGGVFATSNEEDYKEMGKVVFPMFVSNHHLHRLAAMAVTAIEMKLWGSEYARQV
CCCCCCCCCEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VRNAKALAEALASEGFKVVMESKGYTTSHQVVVDVAELGRGTKVAKLLEDAHIIVNKNML
HHHHHHHHHHHHCCCCEEEEECCCCCCCHHHEEEHHHCCCCHHHHHHHHCCEEEEECCCC
PWDRPEDVKDPSGLRLGTQELTRWGMKEGEMKEIARLMKMVVIDKRSPAEVKEKVMEFKK
CCCCCCCCCCCCCCEECHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
EFMEIHYGFKLTREDEVKLLKIMLHAET
HHHHHHCCEEECCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MVYESGEALGIDALREMYPDLNQVLSITINHTVWRKRQTINMIASENVMSPLAMLVYLND
CCCCCCCCCCHHHHHHHCCCHHHHHEEHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MMHRYAEGKPYKRFYQGLIYVDELEVKAQELMGDLLGTKYVDLRPISGTTANATAFRTFT
HHHHHHCCCHHHHHHCCCEEEEHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCEEEEEC
KPGDKAVVAPVQAGAHVSHTRYGTLGALGIEQIEMPFDIEEWNIDVDKARKLIEEVKPKI
CCCCCEEEEECCCCCCCCCCCCCCHHHCCHHHCCCCCCHHHCCCCHHHHHHHHHHCCCCE
VTLGGSLYIFPHPTKEIAEAAHSVGAKVIHDVAHVLGLIVGGVWENPLKLGADVITSSTH
EEECCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCHHHHCCCCC
KTFPGPQGGVFATSNEEDYKEMGKVVFPMFVSNHHLHRLAAMAVTAIEMKLWGSEYARQV
CCCCCCCCCEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VRNAKALAEALASEGFKVVMESKGYTTSHQVVVDVAELGRGTKVAKLLEDAHIIVNKNML
HHHHHHHHHHHHCCCCEEEEECCCCCCCHHHEEEHHHCCCCHHHHHHHHCCEEEEECCCC
PWDRPEDVKDPSGLRLGTQELTRWGMKEGEMKEIARLMKMVVIDKRSPAEVKEKVMEFKK
CCCCCCCCCCCCCCEECHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
EFMEIHYGFKLTREDEVKLLKIMLHAET
HHHHHHCCEEECCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA