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The map label for this gene is gcvT [H]

Identifier: 218883610

GI number: 218883610

Start: 278756

End: 279865

Strand: Direct

Name: gcvT [H]

Synonym: DKAM_0299

Alternate gene names: 218883610

Gene position: 278756-279865 (Clockwise)

Preceding gene: 218883606

Following gene: 218883611

Centisome position: 20.42

GC content: 49.01

Gene sequence:

>1110_bases
ATGTCCAGGATACCTCTACTCGAATACTATATTGAGAAAGGGGCTGAAACAGGTTTATTCGGGGAATGGGAGGTACCATA
CCGCTATACCAACGCTATCGAGGAGCATATGGCTGTGAGAACGAGCGTCGGGCTCTTCGACGTATCCCACATGGGGAGAA
TATTGTTAAAAGGCTCCGATGTATTACCATTCGCCCAGTACATATACACTAAGGACATCAGTAAGACGAAGGAGCACTTT
ATGAGCGGCCCGATGCTAGCATTGAATCAATGGGCTAGAGTAAAAGATGATGAAATGATGTATAAGGTAAGCGATGAAGA
ATGGCTCCTGGTAACAAACGCCTTAGCCAGGGAGTCAATGCTAAACTACTTTGAGAAAGTAAAGAGTGAAAAGGGGTTCA
GAGTTGAGATAGCGGATTTAACGTTCAATTACTCCATGATAGCACTGCAAGGTCCCCGTGCAGCCGAAGTAATGGAGTCG
CTTGGAGCAAAGTGGGCGGGCGACCTCAAGGTCCTGGAGTTCCGTATGAATGAGGAGATACATGGGGTCAAAACCTTCTT
AGTGAGTAGAAGCGGATGGACTGGTGAGGATGGATTTGAGATCTGGGGTGAACACGGTGAGATGAGGAAGCTGGTTGATC
TACTTGTCTCGAAAGGGGTTAAACTAGCCGGGTTAATAGCCAGGGACACCCTGCGTATAGAAATGGGCTTCGTGCTGGGC
GGGCACGAGTACGGTGAGGACCCGGTGAAATTCCCATGCGCCCTAAGCCTGAGATACGGTCTCGGCGCGATCACATGGGA
GAAGAGGGGCTTCGTAGGCGAGGAAGCATTAAGGGCCTGTAGACGTGAGGGTGTTAGATGGATTAGAGTAGGGTTAAAGC
TGGGTAAGGAGGCCGGTAGACTGTTTCCACGGCAGGATGCTGGAGTATACTCTGATGATGTATGGGTTGGATGGGTTACC
AGTGGAACCTACTCCCCAGTCCTCAACAGAGGTATAGCGATGGCTTATGTAGACGCTAGGTACGCTGTATTCGGTGAGGA
GTTAGCAGTGGATATAAGGGGGAAGCGATACCCTGCTAAAATTGTTGACTTTCCATTCATAAAGAAATGA

Upstream 100 bases:

>100_bases
CCCAGTAAACAAATAATGGTTTCGAGGTATATATAGTTATAACTGTGTATGGAACCTATAAATACTTAGATAGATAACAT
TAAAACTATGGTGTATAAAA

Downstream 100 bases:

>100_bases
GTATTTATAGGATCGTTAAGACATAGTAAGGTAAATGTAGGTGGATTGAAATTGGTATATGAATCGGGTGAAGCCCTAGG
CATAGATGCTCTTAGAGAAA

Product: glycine cleavage system aminomethyltransferase T

Products: NA

Alternate protein names: Glycine cleavage system T protein [H]

Number of amino acids: Translated: 369; Mature: 368

Protein sequence:

>369_residues
MSRIPLLEYYIEKGAETGLFGEWEVPYRYTNAIEEHMAVRTSVGLFDVSHMGRILLKGSDVLPFAQYIYTKDISKTKEHF
MSGPMLALNQWARVKDDEMMYKVSDEEWLLVTNALARESMLNYFEKVKSEKGFRVEIADLTFNYSMIALQGPRAAEVMES
LGAKWAGDLKVLEFRMNEEIHGVKTFLVSRSGWTGEDGFEIWGEHGEMRKLVDLLVSKGVKLAGLIARDTLRIEMGFVLG
GHEYGEDPVKFPCALSLRYGLGAITWEKRGFVGEEALRACRREGVRWIRVGLKLGKEAGRLFPRQDAGVYSDDVWVGWVT
SGTYSPVLNRGIAMAYVDARYAVFGEELAVDIRGKRYPAKIVDFPFIKK

Sequences:

>Translated_369_residues
MSRIPLLEYYIEKGAETGLFGEWEVPYRYTNAIEEHMAVRTSVGLFDVSHMGRILLKGSDVLPFAQYIYTKDISKTKEHF
MSGPMLALNQWARVKDDEMMYKVSDEEWLLVTNALARESMLNYFEKVKSEKGFRVEIADLTFNYSMIALQGPRAAEVMES
LGAKWAGDLKVLEFRMNEEIHGVKTFLVSRSGWTGEDGFEIWGEHGEMRKLVDLLVSKGVKLAGLIARDTLRIEMGFVLG
GHEYGEDPVKFPCALSLRYGLGAITWEKRGFVGEEALRACRREGVRWIRVGLKLGKEAGRLFPRQDAGVYSDDVWVGWVT
SGTYSPVLNRGIAMAYVDARYAVFGEELAVDIRGKRYPAKIVDFPFIKK
>Mature_368_residues
SRIPLLEYYIEKGAETGLFGEWEVPYRYTNAIEEHMAVRTSVGLFDVSHMGRILLKGSDVLPFAQYIYTKDISKTKEHFM
SGPMLALNQWARVKDDEMMYKVSDEEWLLVTNALARESMLNYFEKVKSEKGFRVEIADLTFNYSMIALQGPRAAEVMESL
GAKWAGDLKVLEFRMNEEIHGVKTFLVSRSGWTGEDGFEIWGEHGEMRKLVDLLVSKGVKLAGLIARDTLRIEMGFVLGG
HEYGEDPVKFPCALSLRYGLGAITWEKRGFVGEEALRACRREGVRWIRVGLKLGKEAGRLFPRQDAGVYSDDVWVGWVTS
GTYSPVLNRGIAMAYVDARYAVFGEELAVDIRGKRYPAKIVDFPFIKK

Specific function: The glycine cleavage system catalyzes the degradation of glycine [H]

COG id: COG0404

COG function: function code E; Glycine cleavage system T protein (aminomethyltransferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gcvT family [H]

Homologues:

Organism=Homo sapiens, GI44662838, Length=373, Percent_Identity=30.5630026809651, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI257796258, Length=358, Percent_Identity=30.4469273743017, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI257796254, Length=371, Percent_Identity=28.3018867924528, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI257796256, Length=315, Percent_Identity=30.7936507936508, Blast_Score=112, Evalue=7e-25,
Organism=Homo sapiens, GI24797151, Length=391, Percent_Identity=24.8081841432225, Blast_Score=102, Evalue=5e-22,
Organism=Escherichia coli, GI1789272, Length=379, Percent_Identity=33.509234828496, Blast_Score=152, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI17560118, Length=375, Percent_Identity=32.5333333333333, Blast_Score=154, Evalue=8e-38,
Organism=Caenorhabditis elegans, GI71994045, Length=357, Percent_Identity=23.8095238095238, Blast_Score=78, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI71994052, Length=357, Percent_Identity=23.8095238095238, Blast_Score=78, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI32563613, Length=349, Percent_Identity=21.2034383954155, Blast_Score=66, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6320222, Length=388, Percent_Identity=31.1855670103093, Blast_Score=134, Evalue=3e-32,
Organism=Drosophila melanogaster, GI20129441, Length=378, Percent_Identity=32.5396825396825, Blast_Score=159, Evalue=2e-39,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013977
- InterPro:   IPR006222
- InterPro:   IPR006223
- InterPro:   IPR022903 [H]

Pfam domain/function: PF01571 GCV_T; PF08669 GCV_T_C [H]

EC number: =2.1.2.10 [H]

Molecular weight: Translated: 41891; Mature: 41760

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRIPLLEYYIEKGAETGLFGEWEVPYRYTNAIEEHMAVRTSVGLFDVSHMGRILLKGSD
CCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEHHHCCEEEECCCC
VLPFAQYIYTKDISKTKEHFMSGPMLALNQWARVKDDEMMYKVSDEEWLLVTNALARESM
CCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCEEEEECCCCEEEEEHHHHHHHH
LNYFEKVKSEKGFRVEIADLTFNYSMIALQGPRAAEVMESLGAKWAGDLKVLEFRMNEEI
HHHHHHHHCCCCCEEEEEEEEEEEEEEEECCCCHHHHHHHHCCCCCCCEEEEEEECCCCH
HGVKTFLVSRSGWTGEDGFEIWGEHGEMRKLVDLLVSKGVKLAGLIARDTLRIEMGFVLG
HHEEEEEEECCCCCCCCCHHEECCCCHHHHHHHHHHHCCCCEEEEEECCEEEEEEEEEEC
GHEYGEDPVKFPCALSLRYGLGAITWEKRGFVGEEALRACRREGVRWIRVGLKLGKEAGR
CCCCCCCCCCCCEEEEEECCCCEEEECCCCCCCHHHHHHHHHCCCCEEEHHHHHCHHHHC
LFPRQDAGVYSDDVWVGWVTSGTYSPVLNRGIAMAYVDARYAVFGEELAVDIRGKRYPAK
CCCCCCCCCCCCCEEEEEEECCCCCHHHHCCEEEEEECCHHEEECCEEEEEECCCCCCHH
IVDFPFIKK
HCCCCCCCC
>Mature Secondary Structure 
SRIPLLEYYIEKGAETGLFGEWEVPYRYTNAIEEHMAVRTSVGLFDVSHMGRILLKGSD
CCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEHHHCCEEEECCCC
VLPFAQYIYTKDISKTKEHFMSGPMLALNQWARVKDDEMMYKVSDEEWLLVTNALARESM
CCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCEEEEECCCCEEEEEHHHHHHHH
LNYFEKVKSEKGFRVEIADLTFNYSMIALQGPRAAEVMESLGAKWAGDLKVLEFRMNEEI
HHHHHHHHCCCCCEEEEEEEEEEEEEEEECCCCHHHHHHHHCCCCCCCEEEEEEECCCCH
HGVKTFLVSRSGWTGEDGFEIWGEHGEMRKLVDLLVSKGVKLAGLIARDTLRIEMGFVLG
HHEEEEEEECCCCCCCCCHHEECCCCHHHHHHHHHHHCCCCEEEEEECCEEEEEEEEEEC
GHEYGEDPVKFPCALSLRYGLGAITWEKRGFVGEEALRACRREGVRWIRVGLKLGKEAGR
CCCCCCCCCCCCEEEEEECCCCEEEECCCCCCCHHHHHHHHHCCCCEEEHHHHHCHHHHC
LFPRQDAGVYSDDVWVGWVTSGTYSPVLNRGIAMAYVDARYAVFGEELAVDIRGKRYPAK
CCCCCCCCCCCCCEEEEEEECCCCCHHHHCCEEEEEECCHHEEECCEEEEEECCCCCCHH
IVDFPFIKK
HCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10382966 [H]