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The map label for this gene is gcvH [H]

Identifier: 218883612

GI number: 218883612

Start: 281288

End: 281728

Strand: Direct

Name: gcvH [H]

Synonym: DKAM_0301

Alternate gene names: 218883612

Gene position: 281288-281728 (Clockwise)

Preceding gene: 218883611

Following gene: 218883613

Centisome position: 20.6

GC content: 48.53

Gene sequence:

>441_bases
ATGGTGGGATACATGAGTGAAGTCATTGTAGAGGTTAAGGGTAAGAAATACGTTGTTAAAACGGATAGAAGGTACACTGA
GACAGATGAATGGGCTCTCCTCGAGAACGGGAGGATCAGGGTGGGTATAAGCGACTATGCTCAGAAGGAGCTCAAGGACA
TAGTCTCGGTTGAGCTACCCGAGGTCGGGAGAGTAGTGAGGAAAGGGGAGGAGCTGGGTATAATAGACTCCATTAAAGCA
TCATCATCGTATTACGCGCCGGTGAGCGGTAGGATAGTAGAGGTAAATGAAGAGCTATCCACGACCCCGGAGCTCCTTAA
TAAGGACCCCTATGGAGCCGGGTGGATCATCGTTATCGAGCCATCGAACCCGAAGGAGTATGAGGAGCTATATACTCCTG
AGAAATACTCTGAGAAAATAAGGAGCGGGAAACAACACTAG

Upstream 100 bases:

>100_bases
GATACACTACGGCTTCAAGCTGACGAGGGAGGATGAGGTAAAACTATTAAAGATAATGCTTCACGCGGAAACTTAATATT
ATCCGGCCGCATAGCTATAC

Downstream 100 bases:

>100_bases
TTTTTTTAACTCGTTTCTGATTCACTTGATAACCCCGAGAAATAAAGAGGTGTTGTACTTGTTTACACAATTTGTTGAGA
AGCTGAGTGTGAGAGAGCTG

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 146; Mature: 146

Protein sequence:

>146_residues
MVGYMSEVIVEVKGKKYVVKTDRRYTETDEWALLENGRIRVGISDYAQKELKDIVSVELPEVGRVVRKGEELGIIDSIKA
SSSYYAPVSGRIVEVNEELSTTPELLNKDPYGAGWIIVIEPSNPKEYEELYTPEKYSEKIRSGKQH

Sequences:

>Translated_146_residues
MVGYMSEVIVEVKGKKYVVKTDRRYTETDEWALLENGRIRVGISDYAQKELKDIVSVELPEVGRVVRKGEELGIIDSIKA
SSSYYAPVSGRIVEVNEELSTTPELLNKDPYGAGWIIVIEPSNPKEYEELYTPEKYSEKIRSGKQH
>Mature_146_residues
MVGYMSEVIVEVKGKKYVVKTDRRYTETDEWALLENGRIRVGISDYAQKELKDIVSVELPEVGRVVRKGEELGIIDSIKA
SSSYYAPVSGRIVEVNEELSTTPELLNKDPYGAGWIIVIEPSNPKEYEELYTPEKYSEKIRSGKQH

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI49574537, Length=120, Percent_Identity=45.8333333333333, Blast_Score=114, Evalue=4e-26,
Organism=Homo sapiens, GI89057342, Length=120, Percent_Identity=45, Blast_Score=111, Evalue=2e-25,
Organism=Escherichia coli, GI1789271, Length=119, Percent_Identity=44.5378151260504, Blast_Score=104, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17551294, Length=138, Percent_Identity=36.231884057971, Blast_Score=99, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17507493, Length=120, Percent_Identity=38.3333333333333, Blast_Score=99, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6319272, Length=121, Percent_Identity=38.8429752066116, Blast_Score=86, Evalue=2e-18,
Organism=Drosophila melanogaster, GI17865652, Length=130, Percent_Identity=40, Blast_Score=106, Evalue=6e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 16600; Mature: 16600

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVGYMSEVIVEVKGKKYVVKTDRRYTETDEWALLENGRIRVGISDYAQKELKDIVSVELP
CCCCHHHHHEEECCCEEEEECCCCCCCCCCEEEEECCEEEECHHHHHHHHHHHHHCCCCC
EVGRVVRKGEELGIIDSIKASSSYYAPVSGRIVEVNEELSTTPELLNKDPYGAGWIIVIE
HHHHHHHCCCCCCCEECCCCCCCEECCCCCEEEEECCCCCCCHHHHCCCCCCCEEEEEEE
PSNPKEYEELYTPEKYSEKIRSGKQH
CCCCHHHHHHCCCHHHHHHHHCCCCC
>Mature Secondary Structure
MVGYMSEVIVEVKGKKYVVKTDRRYTETDEWALLENGRIRVGISDYAQKELKDIVSVELP
CCCCHHHHHEEECCCEEEEECCCCCCCCCCEEEEECCEEEECHHHHHHHHHHHHHCCCCC
EVGRVVRKGEELGIIDSIKASSSYYAPVSGRIVEVNEELSTTPELLNKDPYGAGWIIVIE
HHHHHHHCCCCCCCEECCCCCCCEECCCCCEEEEECCCCCCCHHHHCCCCCCCEEEEEEE
PSNPKEYEELYTPEKYSEKIRSGKQH
CCCCHHHHHHCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10382966 [H]