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The map label for this gene is proB [H]

Identifier: 218710379

GI number: 218710379

Start: 2585232

End: 2586416

Strand: Reverse

Name: proB [H]

Synonym: VS_2416

Alternate gene names: 218710379

Gene position: 2586416-2585232 (Counterclockwise)

Preceding gene: 218710380

Following gene: 218710378

Centisome position: 78.39

GC content: 47.17

Gene sequence:

>1185_bases
TTGCTAAAACATCACCTCTTATTTGTTTTATCCATAAAAGACTTCATGACAACAAATCATCAAAGCGGGACAACAACACA
GCGTAAAACTGTCGTTGTTAAACTGGGTACCAGTGTCTTAACTGGTGGAACATTGGCATTAGACCGCGCTCATATGGTTG
AGCTGGTTCGTCAATGTGCTGAATTAAAAAAACAAGGCCACTCTGTGGTTATGGTTTCGTCTGGCGCAATTGCAGCCGGA
CGTGAGCACCTTGGTTACCCCGCACTTCCCAACTCGATGGCAAGCAAACAATTGCTTGCGGCAGTTGGGCAAAGTCAGTT
GATTCAAGTTTGGGAGTCTTTGTTTGCTATCTATGGCCTTAAGATTGGCCAGATGCTACTGACTCGTGCTGATCTCGATG
ATCGCGAGCGTTTTCTTAATGCACGTGACACGATCAATGCACTTGTTGAGCACGATATTATTCCGGTAGTAAACGAAAAC
GACGCAGTAGCCACCAACGAAATTAAAGTTGGCGACAATGATAACTTGTCGGCCTTAGTGGGTATTTTGTGCGGTGCTGA
TAAGCTTTTGCTATTAACGGACCAAAAAGGCCTGTTTACGGCTGACCCTCGTAAAGACCCAAATGCTGAGCTCATCAAAG
AAGTGAAAACTATTGATGACACACTGCGCAAGATCGCAGGCGGCAGTGGTACTACGTTAGGTACTGGCGGCATGGCGACA
AAACTGCAGGCGGCTGATATTGCTCGTCGCGCTGGCATCGAAGTAATTATTGCTGCGGGCAGTGCTGAAAATGTAGTGTT
TGACTCACTGAGTGACAACCCACAAGGCACACGTTTCTTGCCTTTAGCGGAAGCGCTTGAAAACCGTAAACGCTGGATTC
TAGCGGGCCCTGCTTCAGCTGGTGACATTGTAGTCGACGACGGCGCAGTAAACGCAGTTAACACCAAAGGCAGCAGCTTG
TTGGCGAAAGGGGTTGTTCGAGTTAAAGGCGAATTCTCTCGTGGTGAAGTCACCCAAGTCACAGACAGCAAAGGCAAAGT
AATTGCGCGTGGTATCGCCAGTTATTCAAGCCAAGACCTAGCAAAAATTGCAGGCAAGCACAGTAAAGATATTGGCGATA
TTCTTGGATACGATTACGGGTCGGAAGTCATTCACCGTGACGACCTTGTTGTAATCCAAGAATAG

Upstream 100 bases:

>100_bases
GAAGAGTCTGTCGAATTCGTCTGATTTTAAAGGTTACTCTTCTATATAAAGCAAAAAGGTGCGATTTCGCACCTTTTCTG
CTTGTGAATTCCCCTTTTGA

Downstream 100 bases:

>100_bases
CTCGTGATGAGAAGGCGAATCACCTTTTTGCATCCAAAGACAGACAGAATTTAGGGAGAGTTAAACGTGGATTTAACTAA
CATGGGTATTGCAGCAAAAG

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MLKHHLLFVLSIKDFMTTNHQSGTTTQRKTVVVKLGTSVLTGGTLALDRAHMVELVRQCAELKKQGHSVVMVSSGAIAAG
REHLGYPALPNSMASKQLLAAVGQSQLIQVWESLFAIYGLKIGQMLLTRADLDDRERFLNARDTINALVEHDIIPVVNEN
DAVATNEIKVGDNDNLSALVGILCGADKLLLLTDQKGLFTADPRKDPNAELIKEVKTIDDTLRKIAGGSGTTLGTGGMAT
KLQAADIARRAGIEVIIAAGSAENVVFDSLSDNPQGTRFLPLAEALENRKRWILAGPASAGDIVVDDGAVNAVNTKGSSL
LAKGVVRVKGEFSRGEVTQVTDSKGKVIARGIASYSSQDLAKIAGKHSKDIGDILGYDYGSEVIHRDDLVVIQE

Sequences:

>Translated_394_residues
MLKHHLLFVLSIKDFMTTNHQSGTTTQRKTVVVKLGTSVLTGGTLALDRAHMVELVRQCAELKKQGHSVVMVSSGAIAAG
REHLGYPALPNSMASKQLLAAVGQSQLIQVWESLFAIYGLKIGQMLLTRADLDDRERFLNARDTINALVEHDIIPVVNEN
DAVATNEIKVGDNDNLSALVGILCGADKLLLLTDQKGLFTADPRKDPNAELIKEVKTIDDTLRKIAGGSGTTLGTGGMAT
KLQAADIARRAGIEVIIAAGSAENVVFDSLSDNPQGTRFLPLAEALENRKRWILAGPASAGDIVVDDGAVNAVNTKGSSL
LAKGVVRVKGEFSRGEVTQVTDSKGKVIARGIASYSSQDLAKIAGKHSKDIGDILGYDYGSEVIHRDDLVVIQE
>Mature_394_residues
MLKHHLLFVLSIKDFMTTNHQSGTTTQRKTVVVKLGTSVLTGGTLALDRAHMVELVRQCAELKKQGHSVVMVSSGAIAAG
REHLGYPALPNSMASKQLLAAVGQSQLIQVWESLFAIYGLKIGQMLLTRADLDDRERFLNARDTINALVEHDIIPVVNEN
DAVATNEIKVGDNDNLSALVGILCGADKLLLLTDQKGLFTADPRKDPNAELIKEVKTIDDTLRKIAGGSGTTLGTGGMAT
KLQAADIARRAGIEVIIAAGSAENVVFDSLSDNPQGTRFLPLAEALENRKRWILAGPASAGDIVVDDGAVNAVNTKGSSL
LAKGVVRVKGEFSRGEVTQVTDSKGKVIARGIASYSSQDLAKIAGKHSKDIGDILGYDYGSEVIHRDDLVVIQE

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI21361368, Length=283, Percent_Identity=31.8021201413428, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI62912457, Length=281, Percent_Identity=31.6725978647687, Blast_Score=121, Evalue=1e-27,
Organism=Escherichia coli, GI1786437, Length=362, Percent_Identity=65.1933701657459, Blast_Score=476, Evalue=1e-136,
Organism=Caenorhabditis elegans, GI17569967, Length=267, Percent_Identity=34.0823970037453, Blast_Score=133, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17569969, Length=267, Percent_Identity=34.0823970037453, Blast_Score=133, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6321822, Length=416, Percent_Identity=36.0576923076923, Blast_Score=224, Evalue=3e-59,
Organism=Saccharomyces cerevisiae, GI6320506, Length=417, Percent_Identity=35.9712230215827, Blast_Score=221, Evalue=2e-58,
Organism=Drosophila melanogaster, GI21357643, Length=291, Percent_Identity=33.6769759450172, Blast_Score=129, Evalue=4e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 42007; Mature: 42007

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS50890 PUA ; PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKHHLLFVLSIKDFMTTNHQSGTTTQRKTVVVKLGTSVLTGGTLALDRAHMVELVRQCA
CCCCEEEEEEEHHHHHHCCCCCCCCCCCEEEEEEECCHHHCCCCHHHHHHHHHHHHHHHH
ELKKQGHSVVMVSSGAIAAGREHLGYPALPNSMASKQLLAAVGQSQLIQVWESLFAIYGL
HHHHCCCEEEEEECCCCCCCHHHCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
KIGQMLLTRADLDDRERFLNARDTINALVEHDIIPVVNENDAVATNEIKVGDNDNLSALV
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEECEEEECCCCCHHHHH
GILCGADKLLLLTDQKGLFTADPRKDPNAELIKEVKTIDDTLRKIAGGSGTTLGTGGMAT
HHHHCCCEEEEEECCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEECCCCCHH
KLQAADIARRAGIEVIIAAGSAENVVFDSLSDNPQGTRFLPLAEALENRKRWILAGPASA
HHHHHHHHHHCCCEEEEECCCCCCEEEECCCCCCCCCEEEHHHHHHHCCCEEEEECCCCC
GDIVVDDGAVNAVNTKGSSLLAKGVVRVKGEFSRGEVTQVTDSKGKVIARGIASYSSQDL
CCEEEECCCEEEECCCCHHHHHHCCEEEECCCCCCCEEEEECCCCCEEEHHHHHCCHHHH
AKIAGKHSKDIGDILGYDYGSEVIHRDDLVVIQE
HHHHCCCCCCHHHHHCCCCCCCCEECCCEEEEEC
>Mature Secondary Structure
MLKHHLLFVLSIKDFMTTNHQSGTTTQRKTVVVKLGTSVLTGGTLALDRAHMVELVRQCA
CCCCEEEEEEEHHHHHHCCCCCCCCCCCEEEEEEECCHHHCCCCHHHHHHHHHHHHHHHH
ELKKQGHSVVMVSSGAIAAGREHLGYPALPNSMASKQLLAAVGQSQLIQVWESLFAIYGL
HHHHCCCEEEEEECCCCCCCHHHCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
KIGQMLLTRADLDDRERFLNARDTINALVEHDIIPVVNENDAVATNEIKVGDNDNLSALV
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEECEEEECCCCCHHHHH
GILCGADKLLLLTDQKGLFTADPRKDPNAELIKEVKTIDDTLRKIAGGSGTTLGTGGMAT
HHHHCCCEEEEEECCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEECCCCCHH
KLQAADIARRAGIEVIIAAGSAENVVFDSLSDNPQGTRFLPLAEALENRKRWILAGPASA
HHHHHHHHHHCCCEEEEECCCCCCEEEECCCCCCCCCEEEHHHHHHHCCCEEEEECCCCC
GDIVVDDGAVNAVNTKGSSLLAKGVVRVKGEFSRGEVTQVTDSKGKVIARGIASYSSQDL
CCEEEECCCEEEECCCCHHHHHHCCEEEECCCCCCCEEEEECCCCCEEEHHHHHCCHHHH
AKIAGKHSKDIGDILGYDYGSEVIHRDDLVVIQE
HHHHCCCCCCHHHHHCCCCCCCCEECCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA