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The map label for this gene is proA [H]

Identifier: 218710378

GI number: 218710378

Start: 2583915

End: 2585165

Strand: Reverse

Name: proA [H]

Synonym: VS_2415

Alternate gene names: 218710378

Gene position: 2585165-2583915 (Counterclockwise)

Preceding gene: 218710379

Following gene: 218710377

Centisome position: 78.35

GC content: 46.92

Gene sequence:

>1251_bases
GTGGATTTAACTAACATGGGTATTGCAGCAAAAGACGCTGCTTTCCACCTCGCGACCGCATCAACGGCGCAAAAGAATAA
AGCATTGGCGATTATCGCTGATGAGCTAGAAGCAAACGCAGCCACGATTTTAGAAGCAAACGCGAAAGATATCGAACTTG
GCCGTGAAGCGGGTCTGACTGATGCACTGCTTGATCGTCTATTGCTGAACGAAGAACGCTTAACCGGTATCGCTAACGAT
GTGCGTAATGTGATTAGCCTGAATGACCCAGTTGGCAGCGAAATTGACAGTAAGGCACTTGAAAACGGTATGTCACTCTC
TCGCCGCCGCGTACCACTTGGTGTGGTGGGTGTTATCTACGAAGCGCGTCCAAACGTAACCATCGATATTGCAGCACTGT
GTCTCAAAACTGGTAACGCAAGCATCCTACGTGGCGGCAAAGAGACATTCTTCTCAAACATGGAGCTGGTTAAAGTAATC
CAGTCTGCATTAGAGAAAGCGGAACTTCCTGCAGCTTCTGTGCAGTACATCGAGAAACCCGATCGTGAACTTGTCTCTCA
ACTGCTTAAACTGGATGACTACGTGGATATGATCATTCCTCGTGGTGGCGCTGGTCTGCACAAGATGTGTAAAGAGAACA
GTACTATTCCAGTTATCATCGGTGGTTTCGGTATCAGCCATATCTTTGTTGATGAGAGCGCTGATCTTGAGAAGTCTGTT
GATGTGGTTGAAAACTCGAAAGTTCAACGTCCATCTGCATGTAACTCATTGGATACATTATTAGTGCATGAAGCGGTTGC
TGAGGCTTTCCTTGCTAAGCTAACACAGCGTTTAGCAGGCAAAGTAACCTTGGTTGCTGACGCTAGAGCAAAATCATTGC
TAGCAGGTTTTGAAGACCAACGTGATGCAGTTGAAGGTGACTTTGACACTGAGTGGCTAAGCTACACGCTAGGCGTAAAA
GTAGTTGCGGATGTGGCAGAAGCGATTGACCACATGCGCGTACACAACGCGAGTCACTCAGATGCGATTATGACTAACAG
TCTAGAGAGCTCAGAACGCTTTATTAATTCAGTCGGTTCAGCTGCAGTCTATGTGAATGCATCAACACGTTTTACTGATG
GCGCACAGTTTGGTTTGGGTGCAGAAGTTGCTGTGTCTACTCAAAAGCTGCATGCTCGCGGGCCAATGGGCTTAGAAGAG
CTGACAAGTTACAAGTGGGTAGGTAAAGCGAACTATTTAGTTCGCGGTTAA

Upstream 100 bases:

>100_bases
TCACCGTGACGACCTTGTTGTAATCCAAGAATAGCTCGTGATGAGAAGGCGAATCACCTTTTTGCATCCAAAGACAGACA
GAATTTAGGGAGAGTTAAAC

Downstream 100 bases:

>100_bases
CGCTGGTCGTTTTTTGCTCCTCAGCTCATTTTTGAAATGGCGATCGAGTAGCAATAATCAATGAATTACCACACAAAAGG
GCCTTAATTGGCCCTTTTTC

Product: gamma-glutamyl phosphate reductase

Products: NA

Alternate protein names: GPR; Glutamate-5-semialdehyde dehydrogenase; Glutamyl-gamma-semialdehyde dehydrogenase; GSA dehydrogenase [H]

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MDLTNMGIAAKDAAFHLATASTAQKNKALAIIADELEANAATILEANAKDIELGREAGLTDALLDRLLLNEERLTGIAND
VRNVISLNDPVGSEIDSKALENGMSLSRRRVPLGVVGVIYEARPNVTIDIAALCLKTGNASILRGGKETFFSNMELVKVI
QSALEKAELPAASVQYIEKPDRELVSQLLKLDDYVDMIIPRGGAGLHKMCKENSTIPVIIGGFGISHIFVDESADLEKSV
DVVENSKVQRPSACNSLDTLLVHEAVAEAFLAKLTQRLAGKVTLVADARAKSLLAGFEDQRDAVEGDFDTEWLSYTLGVK
VVADVAEAIDHMRVHNASHSDAIMTNSLESSERFINSVGSAAVYVNASTRFTDGAQFGLGAEVAVSTQKLHARGPMGLEE
LTSYKWVGKANYLVRG

Sequences:

>Translated_416_residues
MDLTNMGIAAKDAAFHLATASTAQKNKALAIIADELEANAATILEANAKDIELGREAGLTDALLDRLLLNEERLTGIAND
VRNVISLNDPVGSEIDSKALENGMSLSRRRVPLGVVGVIYEARPNVTIDIAALCLKTGNASILRGGKETFFSNMELVKVI
QSALEKAELPAASVQYIEKPDRELVSQLLKLDDYVDMIIPRGGAGLHKMCKENSTIPVIIGGFGISHIFVDESADLEKSV
DVVENSKVQRPSACNSLDTLLVHEAVAEAFLAKLTQRLAGKVTLVADARAKSLLAGFEDQRDAVEGDFDTEWLSYTLGVK
VVADVAEAIDHMRVHNASHSDAIMTNSLESSERFINSVGSAAVYVNASTRFTDGAQFGLGAEVAVSTQKLHARGPMGLEE
LTSYKWVGKANYLVRG
>Mature_416_residues
MDLTNMGIAAKDAAFHLATASTAQKNKALAIIADELEANAATILEANAKDIELGREAGLTDALLDRLLLNEERLTGIAND
VRNVISLNDPVGSEIDSKALENGMSLSRRRVPLGVVGVIYEARPNVTIDIAALCLKTGNASILRGGKETFFSNMELVKVI
QSALEKAELPAASVQYIEKPDRELVSQLLKLDDYVDMIIPRGGAGLHKMCKENSTIPVIIGGFGISHIFVDESADLEKSV
DVVENSKVQRPSACNSLDTLLVHEAVAEAFLAKLTQRLAGKVTLVADARAKSLLAGFEDQRDAVEGDFDTEWLSYTLGVK
VVADVAEAIDHMRVHNASHSDAIMTNSLESSERFINSVGSAAVYVNASTRFTDGAQFGLGAEVAVSTQKLHARGPMGLEE
LTSYKWVGKANYLVRG

Specific function: Catalyzes the NADPH dependent reduction of L-gamma- glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate [H]

COG id: COG0014

COG function: function code E; Gamma-glutamyl phosphate reductase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gamma-glutamyl phosphate reductase family [H]

Homologues:

Organism=Homo sapiens, GI21361368, Length=407, Percent_Identity=36.6093366093366, Blast_Score=226, Evalue=3e-59,
Organism=Homo sapiens, GI62912457, Length=407, Percent_Identity=36.6093366093366, Blast_Score=226, Evalue=3e-59,
Organism=Escherichia coli, GI1786438, Length=415, Percent_Identity=58.0722891566265, Blast_Score=486, Evalue=1e-138,
Organism=Caenorhabditis elegans, GI17569967, Length=402, Percent_Identity=34.5771144278607, Blast_Score=220, Evalue=1e-57,
Organism=Caenorhabditis elegans, GI17569969, Length=402, Percent_Identity=34.5771144278607, Blast_Score=220, Evalue=1e-57,
Organism=Saccharomyces cerevisiae, GI6324899, Length=428, Percent_Identity=37.1495327102804, Blast_Score=273, Evalue=5e-74,
Organism=Drosophila melanogaster, GI21357643, Length=407, Percent_Identity=33.6609336609337, Blast_Score=232, Evalue=4e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR000965
- InterPro:   IPR020593
- InterPro:   IPR012134 [H]

Pfam domain/function: PF00171 Aldedh [H]

EC number: =1.2.1.41 [H]

Molecular weight: Translated: 44648; Mature: 44648

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS01223 PROA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLTNMGIAAKDAAFHLATASTAQKNKALAIIADELEANAATILEANAKDIELGREAGLT
CCCCCCCCEECCCCEEEEECCHHHCCCEEEEEECCCCCCCEEEEECCCCHHHCCHHCCCH
DALLDRLLLNEERLTGIANDVRNVISLNDPVGSEIDSKALENGMSLSRRRVPLGVVGVIY
HHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCHHHHHCCCCEEEEEEEE
EARPNVTIDIAALCLKTGNASILRGGKETFFSNMELVKVIQSALEKAELPAASVQYIEKP
ECCCCCEEEEEHHHHHCCCHHHCCCCHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHCCC
DRELVSQLLKLDDYVDMIIPRGGAGLHKMCKENSTIPVIIGGFGISHIFVDESADLEKSV
CHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCEEEEECCCEEEEEECCCCCHHHHH
DVVENSKVQRPSACNSLDTLLVHEAVAEAFLAKLTQRLAGKVTLVADARAKSLLAGFEDQ
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHCCCHH
RDAVEGDFDTEWLSYTLGVKVVADVAEAIDHMRVHNASHSDAIMTNSLESSERFINSVGS
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHHHCCC
AAVYVNASTRFTDGAQFGLGAEVAVSTQKLHARGPMGLEELTSYKWVGKANYLVRG
EEEEEECCCCCCCCCCCCCCCCEEEEHHHHHCCCCCCHHHHHCCCCCCCCCCEECC
>Mature Secondary Structure
MDLTNMGIAAKDAAFHLATASTAQKNKALAIIADELEANAATILEANAKDIELGREAGLT
CCCCCCCCEECCCCEEEEECCHHHCCCEEEEEECCCCCCCEEEEECCCCHHHCCHHCCCH
DALLDRLLLNEERLTGIANDVRNVISLNDPVGSEIDSKALENGMSLSRRRVPLGVVGVIY
HHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCHHHHHCCCCEEEEEEEE
EARPNVTIDIAALCLKTGNASILRGGKETFFSNMELVKVIQSALEKAELPAASVQYIEKP
ECCCCCEEEEEHHHHHCCCHHHCCCCHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHCCC
DRELVSQLLKLDDYVDMIIPRGGAGLHKMCKENSTIPVIIGGFGISHIFVDESADLEKSV
CHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCEEEEECCCEEEEEECCCCCHHHHH
DVVENSKVQRPSACNSLDTLLVHEAVAEAFLAKLTQRLAGKVTLVADARAKSLLAGFEDQ
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHCCCHH
RDAVEGDFDTEWLSYTLGVKVVADVAEAIDHMRVHNASHSDAIMTNSLESSERFINSVGS
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHHHCCC
AAVYVNASTRFTDGAQFGLGAEVAVSTQKLHARGPMGLEELTSYKWVGKANYLVRG
EEEEEECCCCCCCCCCCCCCCCEEEEHHHHHCCCCCCHHHHHCCCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]