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The map label for this gene is ribH [H]

Identifier: 218710373

GI number: 218710373

Start: 2579494

End: 2580138

Strand: Reverse

Name: ribH [H]

Synonym: VS_2410

Alternate gene names: 218710373

Gene position: 2580138-2579494 (Counterclockwise)

Preceding gene: 218710374

Following gene: 218710372

Centisome position: 78.2

GC content: 42.95

Gene sequence:

>645_bases
GTGTTGAATACACAAGTTTGGCTACTTGCTGCTCCAATATGGTTGGCAGCAGCGCTGCGTTATCATCTTTACATCTCCGG
TGTCTGTTCTTCTAAATTAGCATTAGATATTGCTCACAGTTTTGTGCTAGAATCCGGCGATTCTCACTTGATGAAAATAG
TTAAAGGAAGGCTTATGAAAGTGATCGAGGGTGGCTTCCCAGCGCCAAATGCAAAAATTGCTATCGTTATTGCTCGTTTC
AACAGTTTTATTAACGAAAGTTTACTTTCTGGTGCAATCGATACTTTAAAGCGTCATGGACAAGTAAGCGAAGACAACAT
CACTGTTGTTCGTTGCCCTGGTGCAGTAGAACTTCCACTTGTAGCGCAACGCGTTGCAAAAACGGGTAAGTTCGATGCGA
TTGTATCTCTTGGTACAGTAATCCGTGGCGGTACACCTCACTTTGACTATGTTTGTAGTGAATGTAATAAAGGTTTGGCA
CAAGTGTCTCTGGAATTTTCTCTTCCAGTAGCGTTTGGTGTTCTTACTGTTGATACGATCGATCAAGCTATTGAACGCGC
AGGAACCAAGGCTGGTAATAAGGGTGCAGAAGCCGCACTTAGCGCACTTGAGATGATCAACGTTCTTTCTGAAATCGATT
CCTAA

Upstream 100 bases:

>100_bases
GTCTGTCCGTTTATAGCGACATAATTTACCGATGATTTCAGCGGCTCTTTTAGAGGTTAATTTCGATAAAAGAGCAAAGT
AGCCAAGCCCGATTCAAAAC

Downstream 100 bases:

>100_bases
TGGGGGCCAGTGTGAAACCAGCCGCACGTCGTAACGCACGTCAATTTGCTCTACAAGCAATTTATTCTTGGCAAATTACT
AAAGAAAATATTGCTACCGT

Product: 6,7-dimethyl-8-ribityllumazine synthase

Products: NA

Alternate protein names: DMRL synthase; Lumazine synthase; Riboflavin synthase beta chain [H]

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MLNTQVWLLAAPIWLAAALRYHLYISGVCSSKLALDIAHSFVLESGDSHLMKIVKGRLMKVIEGGFPAPNAKIAIVIARF
NSFINESLLSGAIDTLKRHGQVSEDNITVVRCPGAVELPLVAQRVAKTGKFDAIVSLGTVIRGGTPHFDYVCSECNKGLA
QVSLEFSLPVAFGVLTVDTIDQAIERAGTKAGNKGAEAALSALEMINVLSEIDS

Sequences:

>Translated_214_residues
MLNTQVWLLAAPIWLAAALRYHLYISGVCSSKLALDIAHSFVLESGDSHLMKIVKGRLMKVIEGGFPAPNAKIAIVIARF
NSFINESLLSGAIDTLKRHGQVSEDNITVVRCPGAVELPLVAQRVAKTGKFDAIVSLGTVIRGGTPHFDYVCSECNKGLA
QVSLEFSLPVAFGVLTVDTIDQAIERAGTKAGNKGAEAALSALEMINVLSEIDS
>Mature_214_residues
MLNTQVWLLAAPIWLAAALRYHLYISGVCSSKLALDIAHSFVLESGDSHLMKIVKGRLMKVIEGGFPAPNAKIAIVIARF
NSFINESLLSGAIDTLKRHGQVSEDNITVVRCPGAVELPLVAQRVAKTGKFDAIVSLGTVIRGGTPHFDYVCSECNKGLA
QVSLEFSLPVAFGVLTVDTIDQAIERAGTKAGNKGAEAALSALEMINVLSEIDS

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes

COG id: COG0054

COG function: function code H; Riboflavin synthase beta-chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DMRL synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786617, Length=156, Percent_Identity=65.3846153846154, Blast_Score=211, Evalue=4e-56,
Organism=Saccharomyces cerevisiae, GI6324429, Length=149, Percent_Identity=33.5570469798658, Blast_Score=85, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002180 [H]

Pfam domain/function: PF00885 DMRL_synthase [H]

EC number: =2.5.1.9 [H]

Molecular weight: Translated: 22789; Mature: 22789

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNTQVWLLAAPIWLAAALRYHLYISGVCSSKLALDIAHSFVLESGDSHLMKIVKGRLMK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
VIEGGFPAPNAKIAIVIARFNSFINESLLSGAIDTLKRHGQVSEDNITVVRCPGAVELPL
HHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHH
VAQRVAKTGKFDAIVSLGTVIRGGTPHFDYVCSECNKGLAQVSLEFSLPVAFGVLTVDTI
HHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHEEEEEEECCHHHHHHHHHHH
DQAIERAGTKAGNKGAEAALSALEMINVLSEIDS
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLNTQVWLLAAPIWLAAALRYHLYISGVCSSKLALDIAHSFVLESGDSHLMKIVKGRLMK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
VIEGGFPAPNAKIAIVIARFNSFINESLLSGAIDTLKRHGQVSEDNITVVRCPGAVELPL
HHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHH
VAQRVAKTGKFDAIVSLGTVIRGGTPHFDYVCSECNKGLAQVSLEFSLPVAFGVLTVDTI
HHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHEEEEEEECCHHHHHHHHHHH
DQAIERAGTKAGNKGAEAALSALEMINVLSEIDS
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA