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The map label for this gene is nusB [H]

Identifier: 218710372

GI number: 218710372

Start: 2579027

End: 2579494

Strand: Reverse

Name: nusB [H]

Synonym: VS_2409

Alternate gene names: 218710372

Gene position: 2579494-2579027 (Counterclockwise)

Preceding gene: 218710373

Following gene: 218710371

Centisome position: 78.18

GC content: 44.66

Gene sequence:

>468_bases
ATGGGGGCCAGTGTGAAACCAGCCGCACGTCGTAACGCACGTCAATTTGCTCTACAAGCAATTTATTCTTGGCAAATTAC
TAAAGAAAATATTGCTACCGTTGAAGAACAGTTCTTATCTGGTGGTAAGTATGATGAAGAAGAGCATCATGCCGCAGAAC
CTGCTCTAGCTATGCCAGAAACAGACGTTGCATACTTCCGCGACTTACTAACTGGTGTTGCTCTTAGCCACATGGAACTT
GATAGCAAGCTTCGTCCATTCGTATCTCGCCCTATGCAAGATCTGGATTTGATGGAACTAGCGCTTCTACGTTTAGCTAT
GTACGAGATGACTCGTCGCGAAGATGTACCATACAAAGTGGTTATCAACGAAGCTATCGAGCTTGCGAAAGTATTCGCAG
CAGAAGACAGCCATAAGTTTGTTAACGGTGTGCTTGATAAAGCTGCACCGCACGTTCGTAAGAAATAA

Upstream 100 bases:

>100_bases
AAGCTATTGAACGCGCAGGAACCAAGGCTGGTAATAAGGGTGCAGAAGCCGCACTTAGCGCACTTGAGATGATCAACGTT
CTTTCTGAAATCGATTCCTA

Downstream 100 bases:

>100_bases
GACGTTCGTATATTGAATCTAAAGGTCAGCTTTTATGCTGGCCTTTTTTGTAACTAAATTATCGTAATACCAATAATTAC
GGTGATTTGTATGAAGACTA

Product: transcription antitermination protein NusB

Products: NA

Alternate protein names: Protein nusB [H]

Number of amino acids: Translated: 155; Mature: 154

Protein sequence:

>155_residues
MGASVKPAARRNARQFALQAIYSWQITKENIATVEEQFLSGGKYDEEEHHAAEPALAMPETDVAYFRDLLTGVALSHMEL
DSKLRPFVSRPMQDLDLMELALLRLAMYEMTRREDVPYKVVINEAIELAKVFAAEDSHKFVNGVLDKAAPHVRKK

Sequences:

>Translated_155_residues
MGASVKPAARRNARQFALQAIYSWQITKENIATVEEQFLSGGKYDEEEHHAAEPALAMPETDVAYFRDLLTGVALSHMEL
DSKLRPFVSRPMQDLDLMELALLRLAMYEMTRREDVPYKVVINEAIELAKVFAAEDSHKFVNGVLDKAAPHVRKK
>Mature_154_residues
GASVKPAARRNARQFALQAIYSWQITKENIATVEEQFLSGGKYDEEEHHAAEPALAMPETDVAYFRDLLTGVALSHMELD
SKLRPFVSRPMQDLDLMELALLRLAMYEMTRREDVPYKVVINEAIELAKVFAAEDSHKFVNGVLDKAAPHVRKK

Specific function: Involved in the transcription termination process [H]

COG id: COG0781

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nusB family [H]

Homologues:

Organism=Escherichia coli, GI1786618, Length=149, Percent_Identity=54.3624161073826, Blast_Score=148, Evalue=2e-37,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011605
- InterPro:   IPR006027 [H]

Pfam domain/function: PF01029 NusB [H]

EC number: NA

Molecular weight: Translated: 17559; Mature: 17428

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGASVKPAARRNARQFALQAIYSWQITKENIATVEEQFLSGGKYDEEEHHAAEPALAMPE
CCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCC
TDVAYFRDLLTGVALSHMELDSKLRPFVSRPMQDLDLMELALLRLAMYEMTRREDVPYKV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
VINEAIELAKVFAAEDSHKFVNGVLDKAAPHVRKK
HHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
GASVKPAARRNARQFALQAIYSWQITKENIATVEEQFLSGGKYDEEEHHAAEPALAMPE
CCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCC
TDVAYFRDLLTGVALSHMELDSKLRPFVSRPMQDLDLMELALLRLAMYEMTRREDVPYKV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
VINEAIELAKVFAAEDSHKFVNGVLDKAAPHVRKK
HHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA