Click here to switch to the map view.

The map label for this gene is thrS [H]

Identifier: 218709221

GI number: 218709221

Start: 1303920

End: 1305887

Strand: Direct

Name: thrS [H]

Synonym: VS_1228

Alternate gene names: 218709221

Gene position: 1303920-1305887 (Clockwise)

Preceding gene: 218709218

Following gene: 218709222

Centisome position: 39.52

GC content: 45.53

Gene sequence:

>1968_bases
GTGTTGCTTGGTGTGCAACACCACTGTAAAGGACAAATAATGCCAATTATTACTCTTCCTGACGGTAGTCAGCGTCAATT
TGACAACCCTGTATCAACTCTAGATGTTGCCCTATCAATCGGTCCTGGTCTTGCGAAAGCAACCATTGCTGGTCGTGTAG
ACGGCGAGCGTGTTGATGCTTGTGATCTTATCGAAAACGATGCAAGCCTAGAAATCATCACAGCTAAAGATGAAGTTGAT
GGCCTTGAGATCGTTCGTCACTCTTGTGCTCACCTTTTAGGTCACGCGATTAAGCAGCTTTTTCCAGAAGCGAAAATGGC
GATCGGTCCTACCATCGATAACGGTTTCTACTACGATATCGACCTTGAGCACTCTCTAACGCAAGAAGATCTAGAAAAGA
TTGAAAAGCGTATGAAAGAGCTAGCGAAGACCAAGTATCAGGTTGTTAAGAAGAAAGTTAGCTGGCAGGAAGCGCGTGAC
GCATTCGAAGCTCGCGGCGAGACTTACAAAATCGAAATCTTGGATGAGAACGTTTCTAAAGACGATCGTCCAGGCCTGTA
CCATCATGAAGAATACATCGATATGTGTCGTGGTCCCCACGTTCCACATATGGGCTTCTGCCAACACTTCACACTACTTA
ACGTAGCGGGTGCTTACTGGCGTGGTAACAGTGACAACAAGATGCTTCAACGTATCTACGGCACTGCATTCCACGACAAG
AAAGCGCTTAAGGCTCACCTAGTGCGCCTAGAAGAAGCGGCTAAGCGTGATCACCGTAAAATCGGTAAGCACTTAGATCT
ATTCCACATGCAGCAAGAAGCACCAGGCATGGTGTTCTGGCATCACAACGGTTGGACTATCTTCCGTGAGCTAGAAGTAT
TTATTCGTCAGAAGCTGACTGAGTACGATTACCAAGAAGTAAAAGGCCCATTAATGATGGACCGTGTTCTTTGGGAACGC
TCTGGTCACTGGGATAAGTACGCTGAAGCGATGTTCACGACTTCTTCAGAGAACCGTGAATACGCGATTAAGCCAATGAA
CTGCCCTGGTCACGTTCAAATCTTTAACCAAGGTTTGAAATCTTACCGTGATCTACCGTTACGTATGGCTGAGTTTGGCT
CATGTCACCGTAACGAGCCGTCTGGCGCACTTCACGGCATTATGCGTGTTCGTGGCTTCACTCAAGATGATGCTCACGTA
TTCTGTACTGAAGACCAAGTTCAGCAAGAAGTTAAAGCTTGTATTGAAATGGTTTACGATACTTACACAACTTTCGGTTT
TGAAAACATCGTTGTTAAGCTATCTACTCGTCCAGAACAGCGTGTAGGTTCAGACGAAATGTGGGACCGTGCAGAGGCCG
ATCTTAAGCAAGCGCTTGAGGCGATGGAGATTGCATACGAGATTCAAGAAGGCGAGGGTGCGTTCTACGGACCTAAGATT
GAATTTACTTTGCATGATTGTTTGGACCGCGCATGGCAATGTGGTACAGTGCAGCTCGATTTTGCATTACCAGAACGTTT
AGGTGCAACTTACGTAGGTGAAGATAACGAGCGTCACACGCCAGTTATGATTCACCGCGCGATTTTAGGTTCACTAGAAC
GCTTCATCGGTATTCTTATTGAAGAATACGCTGGCTTCTTCCCAACGTGGTTGGCGCCAGAACAAGCAATTGTTATGGGC
ATTACAGACAAACAGTCTGAATATGTACAAGAAATTGCGAAAAAACTGCAAAAAAGTGGATTTAGAGTCAAAGCAGACTT
GAGAAATGAGAAGATTGGCTTTAAAATCCGCGAACATACTTTGAAACGTGTGCCGTTCATGCTTGTGTGTGGTGACCAAG
AAATGGAAGCCGGCGAAATTGCAGTACGTACACGTAAAGGTAAGGACCTTGGCAAATTTAAAGTGGATGACTTTATTTCA
TACATCCAAGCCGAGGTTTCAAGCCGTAAGCTCAATCTGGAGGAATAG

Upstream 100 bases:

>100_bases
ATGGGGCATCTGTATTTGCTTCTATGTGGGTATCTTTATTATGCATATATCTCCGATCAACATACTTGTTGGTTGAAAAA
CATATATTAAACATTTTCAT

Downstream 100 bases:

>100_bases
CTATTAAAGGCGGAAGACGTGGCCAACAACCGGCCAAACAAAACCAGCACCGTTTAAACGGTGACATTCGTGGCGTTCGT
GAAGTGCGTCTAACTGGCGC

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS [H]

Number of amino acids: Translated: 655; Mature: 655

Protein sequence:

>655_residues
MLLGVQHHCKGQIMPIITLPDGSQRQFDNPVSTLDVALSIGPGLAKATIAGRVDGERVDACDLIENDASLEIITAKDEVD
GLEIVRHSCAHLLGHAIKQLFPEAKMAIGPTIDNGFYYDIDLEHSLTQEDLEKIEKRMKELAKTKYQVVKKKVSWQEARD
AFEARGETYKIEILDENVSKDDRPGLYHHEEYIDMCRGPHVPHMGFCQHFTLLNVAGAYWRGNSDNKMLQRIYGTAFHDK
KALKAHLVRLEEAAKRDHRKIGKHLDLFHMQQEAPGMVFWHHNGWTIFRELEVFIRQKLTEYDYQEVKGPLMMDRVLWER
SGHWDKYAEAMFTTSSENREYAIKPMNCPGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGALHGIMRVRGFTQDDAHV
FCTEDQVQQEVKACIEMVYDTYTTFGFENIVVKLSTRPEQRVGSDEMWDRAEADLKQALEAMEIAYEIQEGEGAFYGPKI
EFTLHDCLDRAWQCGTVQLDFALPERLGATYVGEDNERHTPVMIHRAILGSLERFIGILIEEYAGFFPTWLAPEQAIVMG
ITDKQSEYVQEIAKKLQKSGFRVKADLRNEKIGFKIREHTLKRVPFMLVCGDQEMEAGEIAVRTRKGKDLGKFKVDDFIS
YIQAEVSSRKLNLEE

Sequences:

>Translated_655_residues
MLLGVQHHCKGQIMPIITLPDGSQRQFDNPVSTLDVALSIGPGLAKATIAGRVDGERVDACDLIENDASLEIITAKDEVD
GLEIVRHSCAHLLGHAIKQLFPEAKMAIGPTIDNGFYYDIDLEHSLTQEDLEKIEKRMKELAKTKYQVVKKKVSWQEARD
AFEARGETYKIEILDENVSKDDRPGLYHHEEYIDMCRGPHVPHMGFCQHFTLLNVAGAYWRGNSDNKMLQRIYGTAFHDK
KALKAHLVRLEEAAKRDHRKIGKHLDLFHMQQEAPGMVFWHHNGWTIFRELEVFIRQKLTEYDYQEVKGPLMMDRVLWER
SGHWDKYAEAMFTTSSENREYAIKPMNCPGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGALHGIMRVRGFTQDDAHV
FCTEDQVQQEVKACIEMVYDTYTTFGFENIVVKLSTRPEQRVGSDEMWDRAEADLKQALEAMEIAYEIQEGEGAFYGPKI
EFTLHDCLDRAWQCGTVQLDFALPERLGATYVGEDNERHTPVMIHRAILGSLERFIGILIEEYAGFFPTWLAPEQAIVMG
ITDKQSEYVQEIAKKLQKSGFRVKADLRNEKIGFKIREHTLKRVPFMLVCGDQEMEAGEIAVRTRKGKDLGKFKVDDFIS
YIQAEVSSRKLNLEE
>Mature_655_residues
MLLGVQHHCKGQIMPIITLPDGSQRQFDNPVSTLDVALSIGPGLAKATIAGRVDGERVDACDLIENDASLEIITAKDEVD
GLEIVRHSCAHLLGHAIKQLFPEAKMAIGPTIDNGFYYDIDLEHSLTQEDLEKIEKRMKELAKTKYQVVKKKVSWQEARD
AFEARGETYKIEILDENVSKDDRPGLYHHEEYIDMCRGPHVPHMGFCQHFTLLNVAGAYWRGNSDNKMLQRIYGTAFHDK
KALKAHLVRLEEAAKRDHRKIGKHLDLFHMQQEAPGMVFWHHNGWTIFRELEVFIRQKLTEYDYQEVKGPLMMDRVLWER
SGHWDKYAEAMFTTSSENREYAIKPMNCPGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGALHGIMRVRGFTQDDAHV
FCTEDQVQQEVKACIEMVYDTYTTFGFENIVVKLSTRPEQRVGSDEMWDRAEADLKQALEAMEIAYEIQEGEGAFYGPKI
EFTLHDCLDRAWQCGTVQLDFALPERLGATYVGEDNERHTPVMIHRAILGSLERFIGILIEEYAGFFPTWLAPEQAIVMG
ITDKQSEYVQEIAKKLQKSGFRVKADLRNEKIGFKIREHTLKRVPFMLVCGDQEMEAGEIAVRTRKGKDLGKFKVDDFIS
YIQAEVSSRKLNLEE

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI38570111, Length=652, Percent_Identity=39.2638036809816, Blast_Score=457, Evalue=1e-128,
Organism=Homo sapiens, GI38202255, Length=646, Percent_Identity=38.5448916408669, Blast_Score=450, Evalue=1e-126,
Organism=Homo sapiens, GI20070344, Length=660, Percent_Identity=34.8484848484849, Blast_Score=391, Evalue=1e-108,
Organism=Escherichia coli, GI1788013, Length=643, Percent_Identity=76.8273716951789, Blast_Score=1073, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71984184, Length=631, Percent_Identity=39.3026941362916, Blast_Score=463, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI71984192, Length=631, Percent_Identity=39.3026941362916, Blast_Score=463, Evalue=1e-130,
Organism=Saccharomyces cerevisiae, GI6322112, Length=627, Percent_Identity=38.1180223285486, Blast_Score=438, Evalue=1e-123,
Organism=Saccharomyces cerevisiae, GI6322654, Length=398, Percent_Identity=38.4422110552764, Blast_Score=298, Evalue=2e-81,
Organism=Drosophila melanogaster, GI24583839, Length=633, Percent_Identity=40.6003159557662, Blast_Score=484, Evalue=1e-137,
Organism=Drosophila melanogaster, GI24583842, Length=633, Percent_Identity=40.6003159557662, Blast_Score=484, Evalue=1e-137,
Organism=Drosophila melanogaster, GI28574141, Length=633, Percent_Identity=40.6003159557662, Blast_Score=484, Evalue=1e-137,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR012675
- InterPro:   IPR004095
- InterPro:   IPR012676
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF02824 TGS; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD [H]

EC number: =6.1.1.3 [H]

Molecular weight: Translated: 75151; Mature: 75151

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLGVQHHCKGQIMPIITLPDGSQRQFDNPVSTLDVALSIGPGLAKATIAGRVDGERVDA
CCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHEECCCCHHHHHEECCCCCCCCCH
CDLIENDASLEIITAKDEVDGLEIVRHSCAHLLGHAIKQLFPEAKMAIGPTIDNGFYYDI
HHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCEECCCEEEEE
DLEHSLTQEDLEKIEKRMKELAKTKYQVVKKKVSWQEARDAFEARGETYKIEILDENVSK
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCC
DDRPGLYHHEEYIDMCRGPHVPHMGFCQHFTLLNVAGAYWRGNSDNKMLQRIYGTAFHDK
CCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHH
KALKAHLVRLEEAAKRDHRKIGKHLDLFHMQQEAPGMVFWHHNGWTIFRELEVFIRQKLT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEHHHHHHHHHHHHHH
EYDYQEVKGPLMMDRVLWERSGHWDKYAEAMFTTSSENREYAIKPMNCPGHVQIFNQGLK
HCCHHHHCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHH
SYRDLPLRMAEFGSCHRNEPSGALHGIMRVRGFTQDDAHVFCTEDQVQQEVKACIEMVYD
HHHCCCHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHHHHHH
TYTTFGFENIVVKLSTRPEQRVGSDEMWDRAEADLKQALEAMEIAYEIQEGEGAFYGPKI
HHHHCCCEEEEEEECCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEECCEE
EFTLHDCLDRAWQCGTVQLDFALPERLGATYVGEDNERHTPVMIHRAILGSLERFIGILI
EEEHHHHHHHHHCCCCEEEEECCHHHHCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHH
EEYAGFFPTWLAPEQAIVMGITDKQSEYVQEIAKKLQKSGFRVKADLRNEKIGFKIREHT
HHHHCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHH
LKRVPFMLVCGDQEMEAGEIAVRTRKGKDLGKFKVDDFISYIQAEVSSRKLNLEE
HHHCCEEEEECCCCCCCCCEEEEECCCCCCCCEEHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLLGVQHHCKGQIMPIITLPDGSQRQFDNPVSTLDVALSIGPGLAKATIAGRVDGERVDA
CCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHEECCCCHHHHHEECCCCCCCCCH
CDLIENDASLEIITAKDEVDGLEIVRHSCAHLLGHAIKQLFPEAKMAIGPTIDNGFYYDI
HHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCEECCCEEEEE
DLEHSLTQEDLEKIEKRMKELAKTKYQVVKKKVSWQEARDAFEARGETYKIEILDENVSK
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCC
DDRPGLYHHEEYIDMCRGPHVPHMGFCQHFTLLNVAGAYWRGNSDNKMLQRIYGTAFHDK
CCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHH
KALKAHLVRLEEAAKRDHRKIGKHLDLFHMQQEAPGMVFWHHNGWTIFRELEVFIRQKLT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEHHHHHHHHHHHHHH
EYDYQEVKGPLMMDRVLWERSGHWDKYAEAMFTTSSENREYAIKPMNCPGHVQIFNQGLK
HCCHHHHCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHH
SYRDLPLRMAEFGSCHRNEPSGALHGIMRVRGFTQDDAHVFCTEDQVQQEVKACIEMVYD
HHHCCCHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHHHHHH
TYTTFGFENIVVKLSTRPEQRVGSDEMWDRAEADLKQALEAMEIAYEIQEGEGAFYGPKI
HHHHCCCEEEEEEECCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEECCEE
EFTLHDCLDRAWQCGTVQLDFALPERLGATYVGEDNERHTPVMIHRAILGSLERFIGILI
EEEHHHHHHHHHCCCCEEEEECCHHHHCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHH
EEYAGFFPTWLAPEQAIVMGITDKQSEYVQEIAKKLQKSGFRVKADLRNEKIGFKIREHT
HHHHCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHH
LKRVPFMLVCGDQEMEAGEIAVRTRKGKDLGKFKVDDFISYIQAEVSSRKLNLEE
HHHCCEEEEECCCCCCCCCEEEEECCCCCCCCEEHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA