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The map label for this gene is mlc [H]

Identifier: 218709032

GI number: 218709032

Start: 1081020

End: 1082237

Strand: Direct

Name: mlc [H]

Synonym: VS_1038

Alternate gene names: 218709032

Gene position: 1081020-1082237 (Clockwise)

Preceding gene: 218709030

Following gene: 218709033

Centisome position: 32.77

GC content: 45.24

Gene sequence:

>1218_bases
ATGTACATGGCTCAACCGGGCCATATTGATCATATCAAACAGGTCAATGCTGGTCGTGTATATAAACTAATTGACCTTAA
AGGTCCTATTTCTCGTATCGATCTGTCCAAGCAAAGTGAGTTGGCCCCGGCGAGTATTACTAAAATTACCCGTGAACTCA
TTGAAGCTCACCTTATTCATGAAACGACGGTTCAAGAAGCCACGACTCGTGGGCGTCCTGCTGTCGGTCTGCAAACCAAT
AACGAAGGTTGGCAGTTTCTGTCGATGCGTCTTGGTCGCGGATACTTAACGATCGCGCTCCATGAATTGGGCGGCGATGT
ACTTATCGATACTAAGATTGATATTCATGAACGTGACCAAGACGATGTACTTGCGCGTCTTCTGCATGAAATTGATGAGT
TTTTCCAAACTTATGCCGAGCAACTCGATAGGGTAACAAGTATTGCGGTTACGTTACCGGGGCTTGTGAATTCTGAGCAG
GGAATTGTGTTGCAGATGCCACACTACAACGTTAAAAACTTAGCGTTGGGTCCTGAGATCTACAAAGAAACAGGTCTACC
TGTCTTTATTGCCAATGATACCCGAGCGTGGGCATTAGCAGAGAAGCTGTTTGGTCACTCTCAAGATAACGATAACTCCG
TTTTGATCTCGATTCACCACGGAGTAGGCGCAGGGATCATTCTCGACGGCCGAGTATTGCAAGGTCGCCACGGCAATATT
GGTGAGCTGGGTCATATTCAGATCGATAAACAAGGCAAGCTTTGCCATTGTGGTAATCATGGCTGTTTAGAAACGGTTGC
GAGTTCACAGGCTCTCCGTGAGCAAGTCAAAGAAAGATTGGCTAATGGCGAGGCATCATCACTAACGGCGTTTGAAGATG
TCACAATTGAACAGATCTGCGCTGCTGCTGCTGATGGTGATCCACTGGCTGTTGAAGTGATTGAACAGTTAGGTAGTTAC
TTAGGTGCCGCGATAGCGATTGTGATCAACCTTTTCAATCCTGAGAAAATCTTGATAGGTGGTGTTATCAATCAGGCTAA
AAGCGTGTTGTATCCTGCGATTCAAAAGTGTATCGAAGAGCAGAGTTTATCGGTTTACCATCAAGATTTAGAGCTGGTGG
AATCTCGATTCTATAAACAAGCCACCATGCCAGGTGCTGCGCTTGTTAAACAAGCTTTATATGATGGCCAATTGTTAATG
AAAGTGATTGAAGGCTAA

Upstream 100 bases:

>100_bases
ATGTATATACGTGATACGGGTCACGGCGATATACCAAGTGACTTCACAATTACTTCGCAAAGTAAAAAAATTACCGCGTT
GATGATTTCTGGAGCGTAAA

Downstream 100 bases:

>100_bases
CCCTTTTTTTATCGTTTTTAGCCTTCCTTTAACCCTTTCCATGCACTTAAACTATGTATAACAGCATTTGTTGTACTTAG
TTTAAGCATCTGTGTATTTG

Product: transcriptional regulator

Products: NA

Alternate protein names: Making large colonies protein [H]

Number of amino acids: Translated: 405; Mature: 405

Protein sequence:

>405_residues
MYMAQPGHIDHIKQVNAGRVYKLIDLKGPISRIDLSKQSELAPASITKITRELIEAHLIHETTVQEATTRGRPAVGLQTN
NEGWQFLSMRLGRGYLTIALHELGGDVLIDTKIDIHERDQDDVLARLLHEIDEFFQTYAEQLDRVTSIAVTLPGLVNSEQ
GIVLQMPHYNVKNLALGPEIYKETGLPVFIANDTRAWALAEKLFGHSQDNDNSVLISIHHGVGAGIILDGRVLQGRHGNI
GELGHIQIDKQGKLCHCGNHGCLETVASSQALREQVKERLANGEASSLTAFEDVTIEQICAAAADGDPLAVEVIEQLGSY
LGAAIAIVINLFNPEKILIGGVINQAKSVLYPAIQKCIEEQSLSVYHQDLELVESRFYKQATMPGAALVKQALYDGQLLM
KVIEG

Sequences:

>Translated_405_residues
MYMAQPGHIDHIKQVNAGRVYKLIDLKGPISRIDLSKQSELAPASITKITRELIEAHLIHETTVQEATTRGRPAVGLQTN
NEGWQFLSMRLGRGYLTIALHELGGDVLIDTKIDIHERDQDDVLARLLHEIDEFFQTYAEQLDRVTSIAVTLPGLVNSEQ
GIVLQMPHYNVKNLALGPEIYKETGLPVFIANDTRAWALAEKLFGHSQDNDNSVLISIHHGVGAGIILDGRVLQGRHGNI
GELGHIQIDKQGKLCHCGNHGCLETVASSQALREQVKERLANGEASSLTAFEDVTIEQICAAAADGDPLAVEVIEQLGSY
LGAAIAIVINLFNPEKILIGGVINQAKSVLYPAIQKCIEEQSLSVYHQDLELVESRFYKQATMPGAALVKQALYDGQLLM
KVIEG
>Mature_405_residues
MYMAQPGHIDHIKQVNAGRVYKLIDLKGPISRIDLSKQSELAPASITKITRELIEAHLIHETTVQEATTRGRPAVGLQTN
NEGWQFLSMRLGRGYLTIALHELGGDVLIDTKIDIHERDQDDVLARLLHEIDEFFQTYAEQLDRVTSIAVTLPGLVNSEQ
GIVLQMPHYNVKNLALGPEIYKETGLPVFIANDTRAWALAEKLFGHSQDNDNSVLISIHHGVGAGIILDGRVLQGRHGNI
GELGHIQIDKQGKLCHCGNHGCLETVASSQALREQVKERLANGEASSLTAFEDVTIEQICAAAADGDPLAVEVIEQLGSY
LGAAIAIVINLFNPEKILIGGVINQAKSVLYPAIQKCIEEQSLSVYHQDLELVESRFYKQATMPGAALVKQALYDGQLLM
KVIEG

Specific function: Transcriptional repressor that regulates the expression of proteins that are part of the phosphotransferase system for sugar uptake. Regulates the expression of malT [H]

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family [H]

Homologues:

Organism=Homo sapiens, GI190014632, Length=208, Percent_Identity=30.2884615384615, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI4885285, Length=208, Percent_Identity=30.2884615384615, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI298566325, Length=208, Percent_Identity=30.2884615384615, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI298566315, Length=208, Percent_Identity=30.2884615384615, Blast_Score=81, Evalue=2e-15,
Organism=Escherichia coli, GI1787878, Length=402, Percent_Identity=46.2686567164179, Blast_Score=390, Evalue=1e-110,
Organism=Escherichia coli, GI1786891, Length=401, Percent_Identity=44.8877805486284, Blast_Score=343, Evalue=8e-96,
Organism=Escherichia coli, GI87082127, Length=222, Percent_Identity=28.3783783783784, Blast_Score=98, Evalue=9e-22,
Organism=Escherichia coli, GI1790522, Length=214, Percent_Identity=25.7009345794392, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI87081733, Length=223, Percent_Identity=22.4215246636771, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000600
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00480 ROK [H]

EC number: NA

Molecular weight: Translated: 44377; Mature: 44377

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS01125 ROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYMAQPGHIDHIKQVNAGRVYKLIDLKGPISRIDLSKQSELAPASITKITRELIEAHLIH
CCCCCCCCCHHHHHCCCCCEEEEEECCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHH
ETTVQEATTRGRPAVGLQTNNEGWQFLSMRLGRGYLTIALHELGGDVLIDTKIDIHERDQ
HHHHHHHHHCCCCEEEEEECCCCHHHHHHHHCCCEEEEEEECCCCCEEEEEEECCCCCCH
DDVLARLLHEIDEFFQTYAEQLDRVTSIAVTLPGLVNSEQGIVLQMPHYNVKNLALGPEI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCEEEECCCCCCCCEECCHHH
YKETGLPVFIANDTRAWALAEKLFGHSQDNDNSVLISIHHGVGAGIILDGRVLQGRHGNI
HHHCCCEEEEECCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCEEECCEEECCCCCCC
GELGHIQIDKQGKLCHCGNHGCLETVASSQALREQVKERLANGEASSLTAFEDVTIEQIC
CCCCEEEECCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHCCHHHHH
AAAADGDPLAVEVIEQLGSYLGAAIAIVINLFNPEKILIGGVINQAKSVLYPAIQKCIEE
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHH
QSLSVYHQDLELVESRFYKQATMPGAALVKQALYDGQLLMKVIEG
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCHHHHHHHHCC
>Mature Secondary Structure
MYMAQPGHIDHIKQVNAGRVYKLIDLKGPISRIDLSKQSELAPASITKITRELIEAHLIH
CCCCCCCCCHHHHHCCCCCEEEEEECCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHH
ETTVQEATTRGRPAVGLQTNNEGWQFLSMRLGRGYLTIALHELGGDVLIDTKIDIHERDQ
HHHHHHHHHCCCCEEEEEECCCCHHHHHHHHCCCEEEEEEECCCCCEEEEEEECCCCCCH
DDVLARLLHEIDEFFQTYAEQLDRVTSIAVTLPGLVNSEQGIVLQMPHYNVKNLALGPEI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCEEEECCCCCCCCEECCHHH
YKETGLPVFIANDTRAWALAEKLFGHSQDNDNSVLISIHHGVGAGIILDGRVLQGRHGNI
HHHCCCEEEEECCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCEEECCEEECCCCCCC
GELGHIQIDKQGKLCHCGNHGCLETVASSQALREQVKERLANGEASSLTAFEDVTIEQIC
CCCCEEEECCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHCCHHHHH
AAAADGDPLAVEVIEQLGSYLGAAIAIVINLFNPEKILIGGVINQAKSVLYPAIQKCIEE
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHH
QSLSVYHQDLELVESRFYKQATMPGAALVKQALYDGQLLMKVIEG
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7766024; 9097039; 9278503 [H]