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The map label for this gene is ushA [H]

Identifier: 218708760

GI number: 218708760

Start: 777907

End: 779601

Strand: Direct

Name: ushA [H]

Synonym: VS_0757

Alternate gene names: 218708760

Gene position: 777907-779601 (Clockwise)

Preceding gene: 218708759

Following gene: 218708761

Centisome position: 23.58

GC content: 44.84

Gene sequence:

>1695_bases
GTGGATTCCCCTAAAAAGTTCAAAGGTATGACAATGAAGCAACGCCTTATTCTAAAGACAGCACTAAGTGCTGCAATCCT
AGCTACTTTGGCTGGATGTGCGTCTCAATCAGCTCATGATTGGAACCAAGACGAAACTTATAAGCTAACTATCCTGCATA
CGAACGACAACCATGGTCGTTTTTGGCAGAACAAATACGGCGAATACGGCATGTCTGCACGTAAAACGCTTGTTGACCAA
CTTCGTGCAGAAGTTGAAGCAGAAGGCGGTAGCGTGTTGCTTCTATCTGGCGGTGACATCAACACTGGTGTACCAGAGTC
AGACCTTCAGGATGCAGAACCTGATTTTAAAGGTATGAATAAAATTGGTTACGATGCAATGGCATTAGGTAACCACGAGT
TCGATAACTCACTAGACGTACTGCAAAAACAGATCGACTGGGCAAACTTCCCAATGCTATCTGCAAACATCTACGACAAA
GCGACAGGCGAGCGTAAATTCCAAGCTTACGAGATGTTTGAAAAGCAAGGTATCAAGATTGCTGTTATCGGTCTAACGAC
TGAAGATACTGCGAAAATTGGTAACCCAGAATTTATTGCTGGTATCGATTTCCGTGACCCTAAAGAAGAAGCGAAGAAAC
TGATCGCTGAACTTAAAGAAACAGAAAAACCCGATCTTATCTTCGCTGTGACTCACATGGGTCACTACGAAAATGGTCAG
CGTGGTGTTAATGCTCCGGGTGATGTAGCACTAGCTCGTTACCTAAATGAAGGCGATCTAGATATGATCGTTGGTGGTCA
CTCTCAAGAGCCTGTATGTATGGAAGGCCCTAACGTTGCGAAGCAAAACTTCAAGCCGGGTGATGAGTGTAAACCAGACG
TTCAAAACGGTACTTACATCGTTCAAGCTCACGAATGGGGCAAGTACGTAGGCCGTGCTGATTACGAATTCCGTAACGGC
GAACTAGAGATGGTGAGCTACGACTTGGTTCCCGTTAACCTTAAGAAGAAAGTTAAGATTGACGGCAAGAAGCAACGCGT
TCTTATTCAAGATGAAATCGCACAAGATCCAGAGCTGCTTGAATTCCTACGTCCTTTCCAAGAACAGGGCCAAGCTCAGT
TAGAAGTTCAAATCGCTGAGACAAATGGCAAGCTTGAAGGTGACCGTAACGTAGTTCGCTTCCAACAAACTAACCTGGGT
CGTTTGATTGCGACTTCTCACATGGAGCGTGCAAAAGCAGACTTCGCTGTGATGAACTCTGGTGGCGTTCGTGACTCAAT
TGAAGCCGGTGATGTTACGTACAAAGATGTACTGACAGTACAGCCTTTTGCAAACATCCTGACTTACACTGATATGACTG
GTAAAGAAGTTCTAGATTACCTAAACGTGGTGGCGACTAAACCTGTTGATTCAGGTGCTTATGCACAGTTTGCAGGTATT
TCTATGACTGTCGCTAACGGTGAAGTATCGAATGTCTTCATCGGTGGCAAGCAGCTTCGTTTAGACGAAACATACCGTTT
TACGGTACCAAGCTTTAACGCTGCTGGCGGTGACGGTTATCCTAAACTGTCTGATCACCCTGGCTTTGTGAACACAGGTT
TTGTTGATGCTGAAGTACTAAAAGAGTACCTAGAAGCAAATAGCCCTGTTGATGTGAACAAGTACGCGCCTTCAGGTCAA
ATGGTTTACAAGTAA

Upstream 100 bases:

>100_bases
AATCGTCGTGTTACATTTAGAATGTTAATTTAAGATTAGTCATGTTGAAGTCTTATTGTTCGAACTCCAGACAAGGAGTC
GCAATGGATTATAGAAAGCA

Downstream 100 bases:

>100_bases
TTGAACCCAGTTAATTGAATCACTAGGTGCTAAATTAGATTGACTCTAGCACCATGATAAATTTAACTAAAGCGACGCCT
CGGCGTCGCTTTTTGTTTAT

Product: bifunctional UDP-sugar hydrolase/5'-nucleotidase periplasmic precursor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 564; Mature: 564

Protein sequence:

>564_residues
MDSPKKFKGMTMKQRLILKTALSAAILATLAGCASQSAHDWNQDETYKLTILHTNDNHGRFWQNKYGEYGMSARKTLVDQ
LRAEVEAEGGSVLLLSGGDINTGVPESDLQDAEPDFKGMNKIGYDAMALGNHEFDNSLDVLQKQIDWANFPMLSANIYDK
ATGERKFQAYEMFEKQGIKIAVIGLTTEDTAKIGNPEFIAGIDFRDPKEEAKKLIAELKETEKPDLIFAVTHMGHYENGQ
RGVNAPGDVALARYLNEGDLDMIVGGHSQEPVCMEGPNVAKQNFKPGDECKPDVQNGTYIVQAHEWGKYVGRADYEFRNG
ELEMVSYDLVPVNLKKKVKIDGKKQRVLIQDEIAQDPELLEFLRPFQEQGQAQLEVQIAETNGKLEGDRNVVRFQQTNLG
RLIATSHMERAKADFAVMNSGGVRDSIEAGDVTYKDVLTVQPFANILTYTDMTGKEVLDYLNVVATKPVDSGAYAQFAGI
SMTVANGEVSNVFIGGKQLRLDETYRFTVPSFNAAGGDGYPKLSDHPGFVNTGFVDAEVLKEYLEANSPVDVNKYAPSGQ
MVYK

Sequences:

>Translated_564_residues
MDSPKKFKGMTMKQRLILKTALSAAILATLAGCASQSAHDWNQDETYKLTILHTNDNHGRFWQNKYGEYGMSARKTLVDQ
LRAEVEAEGGSVLLLSGGDINTGVPESDLQDAEPDFKGMNKIGYDAMALGNHEFDNSLDVLQKQIDWANFPMLSANIYDK
ATGERKFQAYEMFEKQGIKIAVIGLTTEDTAKIGNPEFIAGIDFRDPKEEAKKLIAELKETEKPDLIFAVTHMGHYENGQ
RGVNAPGDVALARYLNEGDLDMIVGGHSQEPVCMEGPNVAKQNFKPGDECKPDVQNGTYIVQAHEWGKYVGRADYEFRNG
ELEMVSYDLVPVNLKKKVKIDGKKQRVLIQDEIAQDPELLEFLRPFQEQGQAQLEVQIAETNGKLEGDRNVVRFQQTNLG
RLIATSHMERAKADFAVMNSGGVRDSIEAGDVTYKDVLTVQPFANILTYTDMTGKEVLDYLNVVATKPVDSGAYAQFAGI
SMTVANGEVSNVFIGGKQLRLDETYRFTVPSFNAAGGDGYPKLSDHPGFVNTGFVDAEVLKEYLEANSPVDVNKYAPSGQ
MVYK
>Mature_564_residues
MDSPKKFKGMTMKQRLILKTALSAAILATLAGCASQSAHDWNQDETYKLTILHTNDNHGRFWQNKYGEYGMSARKTLVDQ
LRAEVEAEGGSVLLLSGGDINTGVPESDLQDAEPDFKGMNKIGYDAMALGNHEFDNSLDVLQKQIDWANFPMLSANIYDK
ATGERKFQAYEMFEKQGIKIAVIGLTTEDTAKIGNPEFIAGIDFRDPKEEAKKLIAELKETEKPDLIFAVTHMGHYENGQ
RGVNAPGDVALARYLNEGDLDMIVGGHSQEPVCMEGPNVAKQNFKPGDECKPDVQNGTYIVQAHEWGKYVGRADYEFRNG
ELEMVSYDLVPVNLKKKVKIDGKKQRVLIQDEIAQDPELLEFLRPFQEQGQAQLEVQIAETNGKLEGDRNVVRFQQTNLG
RLIATSHMERAKADFAVMNSGGVRDSIEAGDVTYKDVLTVQPFANILTYTDMTGKEVLDYLNVVATKPVDSGAYAQFAGI
SMTVANGEVSNVFIGGKQLRLDETYRFTVPSFNAAGGDGYPKLSDHPGFVNTGFVDAEVLKEYLEANSPVDVNKYAPSGQ
MVYK

Specific function: Degradation of extracellular 5'-nucleotides for nutritional needs [H]

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=549, Percent_Identity=24.5901639344262, Blast_Score=108, Evalue=1e-23,
Organism=Escherichia coli, GI1786687, Length=549, Percent_Identity=61.9307832422586, Blast_Score=730, Evalue=0.0,
Organism=Escherichia coli, GI1790658, Length=501, Percent_Identity=23.75249500998, Blast_Score=91, Evalue=3e-19,
Organism=Drosophila melanogaster, GI19922446, Length=537, Percent_Identity=26.8156424581006, Blast_Score=138, Evalue=9e-33,
Organism=Drosophila melanogaster, GI24654424, Length=537, Percent_Identity=26.8156424581006, Blast_Score=138, Evalue=9e-33,
Organism=Drosophila melanogaster, GI19922444, Length=550, Percent_Identity=24.9090909090909, Blast_Score=132, Evalue=6e-31,
Organism=Drosophila melanogaster, GI24641187, Length=569, Percent_Identity=24.0773286467487, Blast_Score=113, Evalue=4e-25,
Organism=Drosophila melanogaster, GI28573524, Length=564, Percent_Identity=24.468085106383, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24652512, Length=498, Percent_Identity=26.1044176706827, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI19921980, Length=498, Percent_Identity=26.3052208835341, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI161076508, Length=498, Percent_Identity=26.1044176706827, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI221329836, Length=565, Percent_Identity=23.8938053097345, Blast_Score=103, Evalue=2e-22,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR004843 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.5 [H]

Molecular weight: Translated: 62393; Mature: 62393

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00785 5_NUCLEOTIDASE_1 ; PS00786 5_NUCLEOTIDASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSPKKFKGMTMKQRLILKTALSAAILATLAGCASQSAHDWNQDETYKLTILHTNDNHGR
CCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEECCCCCC
FWQNKYGEYGMSARKTLVDQLRAEVEAEGGSVLLLSGGDINTGVPESDLQDAEPDFKGMN
CHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHCCH
KIGYDAMALGNHEFDNSLDVLQKQIDWANFPMLSANIYDKATGERKFQAYEMFEKQGIKI
HCCCCEEEECCCCCCCHHHHHHHHCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHCCCEE
AVIGLTTEDTAKIGNPEFIAGIDFRDPKEEAKKLIAELKETEKPDLIFAVTHMGHYENGQ
EEEEECCCCHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCC
RGVNAPGDVALARYLNEGDLDMIVGGHSQEPVCMEGPNVAKQNFKPGDECKPDVQNGTYI
CCCCCCCHHHHHHHCCCCCEEEEECCCCCCCEEECCCCCHHCCCCCCCCCCCCCCCCEEE
VQAHEWGKYVGRADYEFRNGELEMVSYDLVPVNLKKKVKIDGKKQRVLIQDEIAQDPELL
EEECHHHHHCCCCCCEEECCEEEEEEEEEEEECCCEEEEECCCCCEEEEEHHHCCCHHHH
EFLRPFQEQGQAQLEVQIAETNGKLEGDRNVVRFQQTNLGRLIATSHMERAKADFAVMNS
HHHHHHHHCCCEEEEEEEEECCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCEEEECC
GGVRDSIEAGDVTYKDVLTVQPFANILTYTDMTGKEVLDYLNVVATKPVDSGAYAQFAGI
CCCCCCCCCCCEEHHHEEEECCHHHHHEECCCCHHHHHHHHHHHCCCCCCCCCCEEECCE
SMTVANGEVSNVFIGGKQLRLDETYRFTVPSFNAAGGDGYPKLSDHPGFVNTGFVDAEVL
EEEEECCCEEEEEECCEEEEECCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
KEYLEANSPVDVNKYAPSGQMVYK
HHHHHCCCCCCCCCCCCCCCEEEC
>Mature Secondary Structure
MDSPKKFKGMTMKQRLILKTALSAAILATLAGCASQSAHDWNQDETYKLTILHTNDNHGR
CCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEECCCCCC
FWQNKYGEYGMSARKTLVDQLRAEVEAEGGSVLLLSGGDINTGVPESDLQDAEPDFKGMN
CHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHCCH
KIGYDAMALGNHEFDNSLDVLQKQIDWANFPMLSANIYDKATGERKFQAYEMFEKQGIKI
HCCCCEEEECCCCCCCHHHHHHHHCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHCCCEE
AVIGLTTEDTAKIGNPEFIAGIDFRDPKEEAKKLIAELKETEKPDLIFAVTHMGHYENGQ
EEEEECCCCHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCC
RGVNAPGDVALARYLNEGDLDMIVGGHSQEPVCMEGPNVAKQNFKPGDECKPDVQNGTYI
CCCCCCCHHHHHHHCCCCCEEEEECCCCCCCEEECCCCCHHCCCCCCCCCCCCCCCCEEE
VQAHEWGKYVGRADYEFRNGELEMVSYDLVPVNLKKKVKIDGKKQRVLIQDEIAQDPELL
EEECHHHHHCCCCCCEEECCEEEEEEEEEEEECCCEEEEECCCCCEEEEEHHHCCCHHHH
EFLRPFQEQGQAQLEVQIAETNGKLEGDRNVVRFQQTNLGRLIATSHMERAKADFAVMNS
HHHHHHHHCCCEEEEEEEEECCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCEEEECC
GGVRDSIEAGDVTYKDVLTVQPFANILTYTDMTGKEVLDYLNVVATKPVDSGAYAQFAGI
CCCCCCCCCCCEEHHHEEEECCHHHHHEECCCCHHHHHHHHHHHCCCCCCCCCCEEECCE
SMTVANGEVSNVFIGGKQLRLDETYRFTVPSFNAAGGDGYPKLSDHPGFVNTGFVDAEVL
EEEEECCCEEEEEECCEEEEECCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
KEYLEANSPVDVNKYAPSGQMVYK
HHHHHCCCCCCCCCCCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA