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The map label for this gene is dacA [H]

Identifier: 218708731

GI number: 218708731

Start: 744711

End: 745892

Strand: Reverse

Name: dacA [H]

Synonym: VS_0710

Alternate gene names: 218708731

Gene position: 745892-744711 (Counterclockwise)

Preceding gene: 218708732

Following gene: 218708730

Centisome position: 22.61

GC content: 45.69

Gene sequence:

>1182_bases
ATGATTAAATCTAATAAACTTGTTAAATCGATTTTTGCTACTTCAGTTGCGCTTTCTGCAACGATAGCAACATCGTCATT
CGCCGCTCCTATTATTGTTCCTGACGCTCCTCAAATCGCTGCTAAGGGATTTGTTCTGATGGATTACCATTCAGGCAAAG
TACTCGCAGAGAAAGAGATGAACACTCAACTTTCTCCAGCAAGTTTAACCAAGATGATGACGAGCTACGTAGTCGGCCAA
GAGCTAGAGCGCGGCAACATCAACCTAAACGACGATGTTGTTATCAGTGAAAATGCTTGGGCTAAAAACTTCCCTGATTC
ATCTAAGATGTTCATTGAAGTGGGTACAACCGTTAAAGTTGAAGAGCTGAACCGCGGTATCATTATTCAATCAGGTAACG
ATGCTTGTGTTGCGATGGCTGAACATATTGCTGGCTCTGAAGATGCATTTGTTGACTTAATGAACGCATGGGCGAGCTCT
ATCGGAATGAAAGACACGCACTTTGCTAACGTACACGGTCTAGACAACCCTAACCTTTACTCAACGCCTTACGATATGGC
GCTACTAGGTCAGGCGCTGATTCGCGATGTTCCTGATGAGTACCGTATCTACTCACAAAAGAAATTCACTTACAACGGCA
TCACCCAGTACAACCGCAACGGTCTGTTATGGGATAAGAGCATGAACGTTGATGGCATTAAAACGGGCCACACAAGCAAT
GCAGGTTACAGCCTAGTAAGCTCAGCAACCGAAGGTAAAATGCGCCTAGTTGCTGTTGTTATGGGCACCAAGAATGCTAA
CGCTCGTAAAACAGAAAGCAAAAAACTGCTTAGCTACGGTTTCCGCTTCTTCGAAACAGTGGCACCACACACCGCTGGTG
AAACCTTCGTTGAAGAGAAGATCTGGATGGGTAGCAAGGACACGGTTGCACTCGGTGTCGACGAAGATACGTTCGTGACT
CTGCCTCGTGGCCAAGCTAAGAACCTGAAGGCAAGCTTCGTTCTTGAGAAAGAGCTAGAAGCACCAATCAGCAAAGGCGA
CGTTGTGGGTAAACTATTCTACCAAGTCGACGGTGAAGACGTTGCTGAATACCCACTACTTGCACTTGAAGATGTAGACC
AAGGCAGCCTATTTAGCCGCCTGTGGGATTACCTAGTACTGCTGTTTAAAGGTTTGTTCTAA

Upstream 100 bases:

>100_bases
TCGCTAATTTATCTAACAGTTACTTCTGGTAATCAGCGCTCTGGTGTGACAGAGTGATTCTGTTAATATATGAATAGTTC
ACCTAATAATTGCATTCAAA

Downstream 100 bases:

>100_bases
ATCAGCCAACGGTTTGTTCTACACAAAACCTAAGCTTGCTGTATAGCTAACCTAATGAGTAGGTTCATGATTTTCTCATT
AGCGAAACAAAAGCCGCCAT

Product: putative Penicillin-binding protein

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5; PBP-5 [H]

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MIKSNKLVKSIFATSVALSATIATSSFAAPIIVPDAPQIAAKGFVLMDYHSGKVLAEKEMNTQLSPASLTKMMTSYVVGQ
ELERGNINLNDDVVISENAWAKNFPDSSKMFIEVGTTVKVEELNRGIIIQSGNDACVAMAEHIAGSEDAFVDLMNAWASS
IGMKDTHFANVHGLDNPNLYSTPYDMALLGQALIRDVPDEYRIYSQKKFTYNGITQYNRNGLLWDKSMNVDGIKTGHTSN
AGYSLVSSATEGKMRLVAVVMGTKNANARKTESKKLLSYGFRFFETVAPHTAGETFVEEKIWMGSKDTVALGVDEDTFVT
LPRGQAKNLKASFVLEKELEAPISKGDVVGKLFYQVDGEDVAEYPLLALEDVDQGSLFSRLWDYLVLLFKGLF

Sequences:

>Translated_393_residues
MIKSNKLVKSIFATSVALSATIATSSFAAPIIVPDAPQIAAKGFVLMDYHSGKVLAEKEMNTQLSPASLTKMMTSYVVGQ
ELERGNINLNDDVVISENAWAKNFPDSSKMFIEVGTTVKVEELNRGIIIQSGNDACVAMAEHIAGSEDAFVDLMNAWASS
IGMKDTHFANVHGLDNPNLYSTPYDMALLGQALIRDVPDEYRIYSQKKFTYNGITQYNRNGLLWDKSMNVDGIKTGHTSN
AGYSLVSSATEGKMRLVAVVMGTKNANARKTESKKLLSYGFRFFETVAPHTAGETFVEEKIWMGSKDTVALGVDEDTFVT
LPRGQAKNLKASFVLEKELEAPISKGDVVGKLFYQVDGEDVAEYPLLALEDVDQGSLFSRLWDYLVLLFKGLF
>Mature_393_residues
MIKSNKLVKSIFATSVALSATIATSSFAAPIIVPDAPQIAAKGFVLMDYHSGKVLAEKEMNTQLSPASLTKMMTSYVVGQ
ELERGNINLNDDVVISENAWAKNFPDSSKMFIEVGTTVKVEELNRGIIIQSGNDACVAMAEHIAGSEDAFVDLMNAWASS
IGMKDTHFANVHGLDNPNLYSTPYDMALLGQALIRDVPDEYRIYSQKKFTYNGITQYNRNGLLWDKSMNVDGIKTGHTSN
AGYSLVSSATEGKMRLVAVVMGTKNANARKTESKKLLSYGFRFFETVAPHTAGETFVEEKIWMGSKDTVALGVDEDTFVT
LPRGQAKNLKASFVLEKELEAPISKGDVVGKLFYQVDGEDVAEYPLLALEDVDQGSLFSRLWDYLVLLFKGLF

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein. Note=N-terminal lies in the periplasmic space (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1786851, Length=399, Percent_Identity=51.3784461152882, Blast_Score=425, Evalue=1e-120,
Organism=Escherichia coli, GI1787062, Length=397, Percent_Identity=50.1259445843829, Blast_Score=403, Evalue=1e-113,
Organism=Escherichia coli, GI87082022, Length=368, Percent_Identity=47.0108695652174, Blast_Score=347, Evalue=1e-96,
Organism=Escherichia coli, GI87082054, Length=237, Percent_Identity=25.7383966244726, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 43127; Mature: 43127

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKSNKLVKSIFATSVALSATIATSSFAAPIIVPDAPQIAAKGFVLMDYHSGKVLAEKEM
CCCCHHHHHHHHHHHHHHHEEEECCCCCCCEEECCCCHHHHCCEEEEEECCCCEEEECCC
NTQLSPASLTKMMTSYVVGQELERGNINLNDDVVISENAWAKNFPDSSKMFIEVGTTVKV
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCEEEEEECCEEEE
EELNRGIIIQSGNDACVAMAEHIAGSEDAFVDLMNAWASSIGMKDTHFANVHGLDNPNLY
EECCCCEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCC
STPYDMALLGQALIRDVPDEYRIYSQKKFTYNGITQYNRNGLLWDKSMNVDGIKTGHTSN
CCCHHHHHHHHHHHHHCCHHHHEECCCEEEECCCEEECCCCEEEECCCCCCCEECCCCCC
AGYSLVSSATEGKMRLVAVVMGTKNANARKTESKKLLSYGFRFFETVAPHTAGETFVEEK
CCCHHHHCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
IWMGSKDTVALGVDEDTFVTLPRGQAKNLKASFVLEKELEAPISKGDVVGKLFYQVDGED
HCCCCCCEEEEECCCCEEEEECCCCCCCCEEEEEEEHHHCCCCCCCCCEEEEEEEECCCC
VAEYPLLALEDVDQGSLFSRLWDYLVLLFKGLF
HHHCCEEEEECCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIKSNKLVKSIFATSVALSATIATSSFAAPIIVPDAPQIAAKGFVLMDYHSGKVLAEKEM
CCCCHHHHHHHHHHHHHHHEEEECCCCCCCEEECCCCHHHHCCEEEEEECCCCEEEECCC
NTQLSPASLTKMMTSYVVGQELERGNINLNDDVVISENAWAKNFPDSSKMFIEVGTTVKV
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCEEEEEECCEEEE
EELNRGIIIQSGNDACVAMAEHIAGSEDAFVDLMNAWASSIGMKDTHFANVHGLDNPNLY
EECCCCEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCC
STPYDMALLGQALIRDVPDEYRIYSQKKFTYNGITQYNRNGLLWDKSMNVDGIKTGHTSN
CCCHHHHHHHHHHHHHCCHHHHEECCCEEEECCCEEECCCCEEEECCCCCCCEECCCCCC
AGYSLVSSATEGKMRLVAVVMGTKNANARKTESKKLLSYGFRFFETVAPHTAGETFVEEK
CCCHHHHCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
IWMGSKDTVALGVDEDTFVTLPRGQAKNLKASFVLEKELEAPISKGDVVGKLFYQVDGED
HCCCCCCEEEEECCCCEEEEECCCCCCCCEEEEEEEHHHCCCCCCCCCEEEEEEEECCCC
VAEYPLLALEDVDQGSLFSRLWDYLVLLFKGLF
HHHCCEEEEECCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]