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The map label for this gene is ppnK

Identifier: 218708669

GI number: 218708669

Start: 673218

End: 674102

Strand: Reverse

Name: ppnK

Synonym: VS_0648

Alternate gene names: 218708669

Gene position: 674102-673218 (Counterclockwise)

Preceding gene: 218708674

Following gene: 218708668

Centisome position: 20.43

GC content: 45.76

Gene sequence:

>885_bases
ATGAAAAAGCCATTTGAAGTCATCGCCATTATTGGTAAACCTCGAGATCAGCAAGCAATTCAGACTCATAGAGAGCTCTA
TCAGTGGTTAAGTACTGAGGGTTATCAAGTGTTTGTGGATGACAGACTCGCTACAATTTTAGACGATATCCCACAAGAAC
ATTTTTCTAGCCTAATTGAATTAGGTAAACGCGCCGATCTCGCCATTGTGGTGGGTGGTGACGGAAATATGCTCGGTGCC
GCTAGAATCTTGTCACGTTTCGATATTTCAGTGATTGGCGTAAACCGAGGTAACCTAGGTTTCCTTACCGATCTTAATCC
TGAAAACTTCCAGAGTGCGCTTACTGATGTACTCAAGGGTGAATTCATGGAAGAAGAGCGCTTCTTACTTGAAACTGAAA
TTCATCGTCATGGCCAAATAAAAAGCCATAACGCCGCACTCAACGAAGCAGTACTTCACCCCGGTCAAGTCGCACATATG
ATCGAGTTCGAAGTGTATATCGATGACAGCTTCGCCTTCTCACAGCGTTCTGATGGCTTGATCGTCTCAACACCGACAGG
TTCAACCGCCTATTCGCTTTCTGGTGGTGGTCCTATCCTATCATCAAGCTTAAATGCAATTTCATTAGTACCGATGTTCC
CACACACCCTTTCAAGCCGACCACTTGTGGTGGATGGCAAGCGTCGTATCAAGCTGATCGTATCGCCAGATAACCGCGGT
ACACAGGAAGTCAGCTGCGATGGCCAAATCTCGCTACCAGTTTCTCCTGGCGATGAGATCCACATTTACCAAAGTCCGAA
TGTGCTCAAGCTGATCCACCCTAAAGACTACAACTACTACCATGTTCTACGTAACAAACTAGGCTGGTCGAGTAAGCTGT
TCTAA

Upstream 100 bases:

>100_bases
TGCACCCCAAATTGATATAAAACACTCGCATAGACGTTCAACTTGCCTTTATTATGGGGATGAAGAATTTTGATTCAAGC
CTAATTCGTTTGGAAACGTT

Downstream 100 bases:

>100_bases
AAGAATAAAAGCTCCAGAAGTAACCGAATTATGAAAGGCTGACGACGTTCGTCAGCCTTTTTTATTGCCCATAGAAAGCC
GTAGGCTCTCGCTTCTCTGC

Product: inorganic polyphosphate/ATP-NAD kinase

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MKKPFEVIAIIGKPRDQQAIQTHRELYQWLSTEGYQVFVDDRLATILDDIPQEHFSSLIELGKRADLAIVVGGDGNMLGA
ARILSRFDISVIGVNRGNLGFLTDLNPENFQSALTDVLKGEFMEEERFLLETEIHRHGQIKSHNAALNEAVLHPGQVAHM
IEFEVYIDDSFAFSQRSDGLIVSTPTGSTAYSLSGGGPILSSSLNAISLVPMFPHTLSSRPLVVDGKRRIKLIVSPDNRG
TQEVSCDGQISLPVSPGDEIHIYQSPNVLKLIHPKDYNYYHVLRNKLGWSSKLF

Sequences:

>Translated_294_residues
MKKPFEVIAIIGKPRDQQAIQTHRELYQWLSTEGYQVFVDDRLATILDDIPQEHFSSLIELGKRADLAIVVGGDGNMLGA
ARILSRFDISVIGVNRGNLGFLTDLNPENFQSALTDVLKGEFMEEERFLLETEIHRHGQIKSHNAALNEAVLHPGQVAHM
IEFEVYIDDSFAFSQRSDGLIVSTPTGSTAYSLSGGGPILSSSLNAISLVPMFPHTLSSRPLVVDGKRRIKLIVSPDNRG
TQEVSCDGQISLPVSPGDEIHIYQSPNVLKLIHPKDYNYYHVLRNKLGWSSKLF
>Mature_294_residues
MKKPFEVIAIIGKPRDQQAIQTHRELYQWLSTEGYQVFVDDRLATILDDIPQEHFSSLIELGKRADLAIVVGGDGNMLGA
ARILSRFDISVIGVNRGNLGFLTDLNPENFQSALTDVLKGEFMEEERFLLETEIHRHGQIKSHNAALNEAVLHPGQVAHM
IEFEVYIDDSFAFSQRSDGLIVSTPTGSTAYSLSGGGPILSSSLNAISLVPMFPHTLSSRPLVVDGKRRIKLIVSPDNRG
TQEVSCDGQISLPVSPGDEIHIYQSPNVLKLIHPKDYNYYHVLRNKLGWSSKLF

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family

Homologues:

Organism=Homo sapiens, GI55743112, Length=330, Percent_Identity=30, Blast_Score=110, Evalue=2e-24,
Organism=Escherichia coli, GI1788968, Length=294, Percent_Identity=58.5034013605442, Blast_Score=353, Evalue=6e-99,
Organism=Saccharomyces cerevisiae, GI6320794, Length=242, Percent_Identity=33.0578512396694, Blast_Score=139, Evalue=7e-34,
Organism=Saccharomyces cerevisiae, GI6322509, Length=241, Percent_Identity=31.1203319502075, Blast_Score=123, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6325068, Length=213, Percent_Identity=30.5164319248826, Blast_Score=101, Evalue=2e-22,
Organism=Drosophila melanogaster, GI281363321, Length=371, Percent_Identity=26.6846361185984, Blast_Score=108, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24653424, Length=371, Percent_Identity=26.6846361185984, Blast_Score=108, Evalue=3e-24,
Organism=Drosophila melanogaster, GI20129957, Length=371, Percent_Identity=26.6846361185984, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI281363323, Length=371, Percent_Identity=26.6846361185984, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24653422, Length=371, Percent_Identity=26.6846361185984, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI28573826, Length=254, Percent_Identity=31.8897637795276, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI28573832, Length=254, Percent_Identity=31.8897637795276, Blast_Score=107, Evalue=6e-24,
Organism=Drosophila melanogaster, GI28573828, Length=254, Percent_Identity=32.2834645669291, Blast_Score=107, Evalue=7e-24,
Organism=Drosophila melanogaster, GI28573830, Length=254, Percent_Identity=31.8897637795276, Blast_Score=107, Evalue=8e-24,
Organism=Drosophila melanogaster, GI161077047, Length=254, Percent_Identity=31.8897637795276, Blast_Score=107, Evalue=8e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PPNK_VIBSL (B7VJW6)

Other databases:

- EMBL:   FM954972
- RefSeq:   YP_002416290.1
- ProteinModelPortal:   B7VJW6
- SMR:   B7VJW6
- GeneID:   7160267
- GenomeReviews:   FM954972_GR
- KEGG:   vsp:VS_0648
- HOGENOM:   HBG713904
- OMA:   FESFMID
- ProtClustDB:   PRK03378
- GO:   GO:0005737
- HAMAP:   MF_00361
- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504
- Gene3D:   G3DSA:2.60.200.30
- Gene3D:   G3DSA:3.40.50.10330
- PANTHER:   PTHR20275

Pfam domain/function: PF01513 NAD_kinase; SSF111331 ATP-NAD_kinase_PpnK-typ

EC number: =2.7.1.23

Molecular weight: Translated: 32678; Mature: 32678

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKPFEVIAIIGKPRDQQAIQTHRELYQWLSTEGYQVFVDDRLATILDDIPQEHFSSLIE
CCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHCCHHHHHHHHH
LGKRADLAIVVGGDGNMLGAARILSRFDISVIGVNRGNLGFLTDLNPENFQSALTDVLKG
CCCCCCEEEEECCCCCCHHHHHHHHHCCEEEEEECCCCEEEEECCCHHHHHHHHHHHHHC
EFMEEERFLLETEIHRHGQIKSHNAALNEAVLHPGQVAHMIEFEVYIDDSFAFSQRSDGL
CHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCEEEEEEEEEEECCCEEECCCCCCE
IVSTPTGSTAYSLSGGGPILSSSLNAISLVPMFPHTLSSRPLVVDGKRRIKLIVSPDNRG
EEECCCCCEEEEECCCCCHHHCCCCEEEEEECCCCCCCCCCEEEECCCEEEEEECCCCCC
TQEVSCDGQISLPVSPGDEIHIYQSPNVLKLIHPKDYNYYHVLRNKLGWSSKLF
CCEEECCCEEEEECCCCCEEEEEECCCEEEEECCCCCCHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKKPFEVIAIIGKPRDQQAIQTHRELYQWLSTEGYQVFVDDRLATILDDIPQEHFSSLIE
CCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHCCHHHHHHHHH
LGKRADLAIVVGGDGNMLGAARILSRFDISVIGVNRGNLGFLTDLNPENFQSALTDVLKG
CCCCCCEEEEECCCCCCHHHHHHHHHCCEEEEEECCCCEEEEECCCHHHHHHHHHHHHHC
EFMEEERFLLETEIHRHGQIKSHNAALNEAVLHPGQVAHMIEFEVYIDDSFAFSQRSDGL
CHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCEEEEEEEEEEECCCEEECCCCCCE
IVSTPTGSTAYSLSGGGPILSSSLNAISLVPMFPHTLSSRPLVVDGKRRIKLIVSPDNRG
EEECCCCCEEEEECCCCCHHHCCCCEEEEEECCCCCCCCCCEEEECCCEEEEEECCCCCC
TQEVSCDGQISLPVSPGDEIHIYQSPNVLKLIHPKDYNYYHVLRNKLGWSSKLF
CCEEECCCEEEEECCCCCEEEEEECCCEEEEECCCCCCHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA