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The map label for this gene is 218708656

Identifier: 218708656

GI number: 218708656

Start: 660217

End: 661587

Strand: Reverse

Name: 218708656

Synonym: VS_0634

Alternate gene names: NA

Gene position: 661587-660217 (Counterclockwise)

Preceding gene: 218708657

Following gene: 218708655

Centisome position: 20.05

GC content: 46.1

Gene sequence:

>1371_bases
ATGGAAGTGATTCACCATGGCGGCAAACATACTATCACAGGATCTTGCCACGAATTAAGAGATTCAGGCCAAGCGGTGCT
CATCGATTGTGGCTTGTTTCAAGGTTTAGATCTTCAAGGTAAAAAGCTTCAAGGTGAAGATGCACGCCCTCTTGATATTG
AATTTGAAACGTCCCACCTTAACGCTCTGCTGCTGACTCATACTCACATTGATCATATTGGTCGATTACCTTGGTTGCTC
GCCAGTGGCTTTAACCAACCAATTTATTGCACTCAGGCGACGGCTGAACTCGCCCCTTTAATGATTGAAGACGGCCTAAA
ACTGCAAGGGCTCAGTCATAAACAATCCAAACTCATCCTTAAAAAAATTCATTCATTGATTGCTCCTAAGCCTTATGGGG
AGTGGTTTCCAATTGCCTCTAAACAGCAAAACGACGGAAAGGATCATCATAGGCCAAATACTCTGTATGCTCGTTTTCAA
CCTGCTGGGCATATCTTAGGTTCCGCCTATATTGAGCTTAAGTTACCAAACCAAGAGGTGGTGGTCTTTTCAGGAGACTT
GGGCACAAGTAATACGCCACTGCTGCCGGATCCGAAACCACCACAACAAGCCGACTATTTGTTTATCGAATCGACTTACG
GCACTAGCACGCACGACGATATTGCTTCACGCTCTGAACGCCTCAAAGCAATTATTGACCGTTCTTTACTCGATGGTGGC
GTCATCCTGATCCCGGCATTTAGTATCGGCAGAACACAAGAACTGTTGTTTGATATTGAAAACTTGATCTTTGAGCATCT
ACTCAGCGCCGATATTCCTATCATCCTCGATTCGCCTATGGCAGAGAAAGTAACGCGTTCATACCGACGCTTTAAAGAGT
TATGGGGGCAAGAAGCAAAGCAGCGTCTTGAACTGAAACGTCACCCACTCGCTTTTGAGCAGTGCATTACGGTTGATGGA
CATAGAATGCACAAGAAAATCGTGAATCGCCTCAAGTCGACAGGTGAACCCGCGATTGTGGTCGCAGCTTCTGGAATGTG
TCAGGGCGGCAGGATAATGAACTACTTATCCGCTTTGCTTCCCGATAAACGGACGGATGTGATTTTAGCGGGCTATCAAG
CACACGGAACGCTCGGACGAGAGCTGCAACAAGGCGAAATGCAGGTTTCTATTGATAACAAAGACGTTGAGGTTAATGCT
CAAATTCATGGCATGTCTGGCTATTCTGCTCATGCCGATAAAGAGGATCTCAATCGATTTATAGAGGGCATAGCGACTCC
ACCAAAAGAGGTGCATTTGATTCACGGCGAGCCAAATACTCAGTCTGAGTTCGCTAAAGAATTACAGGCGCGAGGGTTCG
TGGTTGTTTGA

Upstream 100 bases:

>100_bases
TCAACGATCGCAACTCTCATTGTTCTTGTTTTACATGCCGGCGCTTATCCGCAAAATAAAAACAGGCGTTAATGGCTTTA
TAGAGCAATGGGAGTAAACG

Downstream 100 bases:

>100_bases
AACAGGCATCAAACTAGCTACTTTCACTCAGTAAACATGAAGGCCAGAATTATCGAGCCTTCAAAGGCACAGTTTCTTAG
GCCTGCGAGATCACTCGATG

Product: putative exonuclease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MEVIHHGGKHTITGSCHELRDSGQAVLIDCGLFQGLDLQGKKLQGEDARPLDIEFETSHLNALLLTHTHIDHIGRLPWLL
ASGFNQPIYCTQATAELAPLMIEDGLKLQGLSHKQSKLILKKIHSLIAPKPYGEWFPIASKQQNDGKDHHRPNTLYARFQ
PAGHILGSAYIELKLPNQEVVVFSGDLGTSNTPLLPDPKPPQQADYLFIESTYGTSTHDDIASRSERLKAIIDRSLLDGG
VILIPAFSIGRTQELLFDIENLIFEHLLSADIPIILDSPMAEKVTRSYRRFKELWGQEAKQRLELKRHPLAFEQCITVDG
HRMHKKIVNRLKSTGEPAIVVAASGMCQGGRIMNYLSALLPDKRTDVILAGYQAHGTLGRELQQGEMQVSIDNKDVEVNA
QIHGMSGYSAHADKEDLNRFIEGIATPPKEVHLIHGEPNTQSEFAKELQARGFVVV

Sequences:

>Translated_456_residues
MEVIHHGGKHTITGSCHELRDSGQAVLIDCGLFQGLDLQGKKLQGEDARPLDIEFETSHLNALLLTHTHIDHIGRLPWLL
ASGFNQPIYCTQATAELAPLMIEDGLKLQGLSHKQSKLILKKIHSLIAPKPYGEWFPIASKQQNDGKDHHRPNTLYARFQ
PAGHILGSAYIELKLPNQEVVVFSGDLGTSNTPLLPDPKPPQQADYLFIESTYGTSTHDDIASRSERLKAIIDRSLLDGG
VILIPAFSIGRTQELLFDIENLIFEHLLSADIPIILDSPMAEKVTRSYRRFKELWGQEAKQRLELKRHPLAFEQCITVDG
HRMHKKIVNRLKSTGEPAIVVAASGMCQGGRIMNYLSALLPDKRTDVILAGYQAHGTLGRELQQGEMQVSIDNKDVEVNA
QIHGMSGYSAHADKEDLNRFIEGIATPPKEVHLIHGEPNTQSEFAKELQARGFVVV
>Mature_456_residues
MEVIHHGGKHTITGSCHELRDSGQAVLIDCGLFQGLDLQGKKLQGEDARPLDIEFETSHLNALLLTHTHIDHIGRLPWLL
ASGFNQPIYCTQATAELAPLMIEDGLKLQGLSHKQSKLILKKIHSLIAPKPYGEWFPIASKQQNDGKDHHRPNTLYARFQ
PAGHILGSAYIELKLPNQEVVVFSGDLGTSNTPLLPDPKPPQQADYLFIESTYGTSTHDDIASRSERLKAIIDRSLLDGG
VILIPAFSIGRTQELLFDIENLIFEHLLSADIPIILDSPMAEKVTRSYRRFKELWGQEAKQRLELKRHPLAFEQCITVDG
HRMHKKIVNRLKSTGEPAIVVAASGMCQGGRIMNYLSALLPDKRTDVILAGYQAHGTLGRELQQGEMQVSIDNKDVEVNA
QIHGMSGYSAHADKEDLNRFIEGIATPPKEVHLIHGEPNTQSEFAKELQARGFVVV

Specific function: Has endoribonuclease activity towards 23S and 16S rRNA (in vitro) [H]

COG id: COG1236

COG function: function code J; Predicted exonuclease of the beta-lactamase fold involved in RNA processing

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family [H]

Homologues:

Organism=Homo sapiens, GI7706427, Length=448, Percent_Identity=26.7857142857143, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI33300633, Length=451, Percent_Identity=25.7206208425721, Blast_Score=145, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI32566029, Length=436, Percent_Identity=29.5871559633028, Blast_Score=169, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI32564696, Length=472, Percent_Identity=24.7881355932203, Blast_Score=151, Evalue=8e-37,
Organism=Saccharomyces cerevisiae, GI6323307, Length=446, Percent_Identity=24.4394618834081, Blast_Score=146, Evalue=6e-36,
Organism=Drosophila melanogaster, GI24648013, Length=449, Percent_Identity=26.9487750556793, Blast_Score=166, Evalue=4e-41,
Organism=Drosophila melanogaster, GI21358523, Length=445, Percent_Identity=27.1910112359551, Blast_Score=145, Evalue=5e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022712
- InterPro:   IPR001279
- InterPro:   IPR011108 [H]

Pfam domain/function: PF10996 Beta-Casp; PF00753 Lactamase_B; PF07521 RMMBL [H]

EC number: NA

Molecular weight: Translated: 50688; Mature: 50688

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVIHHGGKHTITGSCHELRDSGQAVLIDCGLFQGLDLQGKKLQGEDARPLDIEFETSHL
CCCEECCCCEEECCCHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEECCCC
NALLLTHTHIDHIGRLPWLLASGFNQPIYCTQATAELAPLMIEDGLKLQGLSHKQSKLIL
EEEEEEEHHHHHHHHHHHHHHCCCCCCEEEECCHHHHCCHHHHCCCEECCCCCHHHHHHH
KKIHSLIAPKPYGEWFPIASKQQNDGKDHHRPNTLYARFQPAGHILGSAYIELKLPNQEV
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEEEEEEEECCCCEE
VVFSGDLGTSNTPLLPDPKPPQQADYLFIESTYGTSTHDDIASRSERLKAIIDRSLLDGG
EEEECCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCC
VILIPAFSIGRTQELLFDIENLIFEHLLSADIPIILDSPMAEKVTRSYRRFKELWGQEAK
EEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHH
QRLELKRHPLAFEQCITVDGHRMHKKIVNRLKSTGEPAIVVAASGMCQGGRIMNYLSALL
HHHHHHCCCCHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHC
PDKRTDVILAGYQAHGTLGRELQQGEMQVSIDNKDVEVNAQIHGMSGYSAHADKEDLNRF
CCCCCCEEEEECCCCCCHHHHHHCCCEEEEECCCCEEEEEEEECCCCCCCCCCHHHHHHH
IEGIATPPKEVHLIHGEPNTQSEFAKELQARGFVVV
HHHHCCCCCCEEEEECCCCCHHHHHHHHHHCCCEEC
>Mature Secondary Structure
MEVIHHGGKHTITGSCHELRDSGQAVLIDCGLFQGLDLQGKKLQGEDARPLDIEFETSHL
CCCEECCCCEEECCCHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEECCCC
NALLLTHTHIDHIGRLPWLLASGFNQPIYCTQATAELAPLMIEDGLKLQGLSHKQSKLIL
EEEEEEEHHHHHHHHHHHHHHCCCCCCEEEECCHHHHCCHHHHCCCEECCCCCHHHHHHH
KKIHSLIAPKPYGEWFPIASKQQNDGKDHHRPNTLYARFQPAGHILGSAYIELKLPNQEV
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEEEEEEEECCCCEE
VVFSGDLGTSNTPLLPDPKPPQQADYLFIESTYGTSTHDDIASRSERLKAIIDRSLLDGG
EEEECCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCC
VILIPAFSIGRTQELLFDIENLIFEHLLSADIPIILDSPMAEKVTRSYRRFKELWGQEAK
EEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHH
QRLELKRHPLAFEQCITVDGHRMHKKIVNRLKSTGEPAIVVAASGMCQGGRIMNYLSALL
HHHHHHCCCCHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHC
PDKRTDVILAGYQAHGTLGRELQQGEMQVSIDNKDVEVNAQIHGMSGYSAHADKEDLNRF
CCCCCCEEEEECCCCCCHHHHHHCCCEEEEECCCCEEEEEEEECCCCCCCCCCHHHHHHH
IEGIATPPKEVHLIHGEPNTQSEFAKELQARGFVVV
HHHHCCCCCCEEEEECCCCCHHHHHHHHHHCCCEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA