Click here to switch to the map view.

The map label for this gene is ddg

Identifier: 218696021

GI number: 218696021

Start: 2728023

End: 2728943

Strand: Direct

Name: ddg

Synonym: EC55989_2674

Alternate gene names: 218696021

Gene position: 2728023-2728943 (Clockwise)

Preceding gene: 218696018

Following gene: 218696023

Centisome position: 52.92

GC content: 51.47

Gene sequence:

>921_bases
ATGTTTCCACAATGCAAATTTTCCCGCGAGTTTCTACATCCTCGCTACTGGCTCACATGGTTTGGGCTTGGTGTACTCTG
GCTTTGGGTACAGCTTCCTTATCCTGTTCTCTGCTTTCTCGGCACGCGTATTGGCGCAATGGCGCGACCATTCCTGAAAC
GTCGTGAATCTATCGCCCGTAAAAACCTGGAACTTTGTTTCCCGCAGCATTCTGCGGAAGAACGCGAGAAGATGATTGCC
GAAAACTTTCGTTCACTCGGCATGGCGCTGGTAGAAACCGGCATGGCATGGTTCTGGCCCGACAGTCGCGTACGTAAATG
GTTTGATGTTGAAGGGTTGGATAACCTTAAACGCGCACAAATGCAAAATCGCGGCGTAATGGTTGTCGGCGTCCATTTTA
TGTCGCTGGAACTGGGCGGCCGCGTGATGGGACTGTGCCAACCAATGATGGCTACCTATCGTCCACATAATAATCAGCTG
ATGGAATGGGTGCAGACCCGTGGGCGCATGCGCTCTAACAAAGCGATGATCGGCAGAAATAATCTGCGCGGCATTGTCGG
TGCACTGAAGAAAGGTGAAGCGGTATGGTTTGCTCCCGATCAGGATTATGGTCGTAAAGGCAGCTCCTTCGCGCCGTTCT
TTGCGGTGGAAAATGTCGCCACAACCAATGGCACCTATGTTCTCTCCCGTCTCTCTGGCGCAGCCATGTTGACCGTAACG
ATGGTAAGAAAAGCGGATTACAGCGGATATCGTTTGTTCATCACCCCAGAGATGGAAGGCTACCCGACAGATGAAAATCA
AGCCGCTGCCTATATGAACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCT
TTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAA

Upstream 100 bases:

>100_bases
CAGGAACCATTGTCGTACATGATGGCCCAACCAATTGAGTGTACCGCTGTCGACATGGAATCTTCTGCGATACAACAATT
CGTATCTACAGAAGGTAACT

Downstream 100 bases:

>100_bases
CAAACTGCTCTTACTCTTTCCCTCCTCAAATCGGCCATAATAACCTCATGGTTTTTATGGCTTTTTTCTTAAGGTTTTTT
CCTTCGCTGAATATCTAATT

Product: lipid A biosynthesis palmitoleoyl acyltransferase

Products: KDO2-(palmitoleoyl)-lipid IVA [C]

Alternate protein names: NA

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRIGAMARPFLKRRESIARKNLELCFPQHSAEEREKMIA
ENFRSLGMALVETGMAWFWPDSRVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVENVATTNGTYVLSRLSGAAMLTVT
MVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKIIEKEIMRAPEQYLWIHRRFKTRPVGESSLYI

Sequences:

>Translated_306_residues
MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRIGAMARPFLKRRESIARKNLELCFPQHSAEEREKMIA
ENFRSLGMALVETGMAWFWPDSRVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVENVATTNGTYVLSRLSGAAMLTVT
MVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKIIEKEIMRAPEQYLWIHRRFKTRPVGESSLYI
>Mature_306_residues
MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRIGAMARPFLKRRESIARKNLELCFPQHSAEEREKMIA
ENFRSLGMALVETGMAWFWPDSRVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVENVATTNGTYVLSRLSGAAMLTVT
MVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKIIEKEIMRAPEQYLWIHRRFKTRPVGESSLYI

Specific function: Unknown

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family

Homologues:

Organism=Escherichia coli, GI87082094, Length=306, Percent_Identity=100, Blast_Score=638, Evalue=0.0,
Organism=Escherichia coli, GI1787292, Length=301, Percent_Identity=54.8172757475083, Blast_Score=362, Evalue=1e-101,
Organism=Escherichia coli, GI1788161, Length=307, Percent_Identity=26.0586319218241, Blast_Score=106, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDG_ECOLI (P0ACV2)

Other databases:

- EMBL:   U49787
- EMBL:   U00096
- EMBL:   AP009048
- RefSeq:   AP_002978.1
- RefSeq:   NP_416879.4
- ProteinModelPortal:   P0ACV2
- STRING:   P0ACV2
- EnsemblBacteria:   EBESCT00000003367
- EnsemblBacteria:   EBESCT00000017454
- GeneID:   946847
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW2375
- KEGG:   eco:b2378
- EchoBASE:   EB2738
- EcoGene:   EG12901
- eggNOG:   COG1560
- GeneTree:   EBGT00050000010320
- HOGENOM:   HBG730280
- OMA:   GRAMGLC
- ProtClustDB:   PRK08025
- BioCyc:   EcoCyc:PALMITOTRANS-MONOMER
- BioCyc:   MetaCyc:PALMITOTRANS-MONOMER
- Genevestigator:   P0ACV2
- InterPro:   IPR004960
- InterPro:   IPR011920
- TIGRFAMs:   TIGR02207

Pfam domain/function: PF03279 Lip_A_acyltrans

EC number: NA

Molecular weight: Translated: 35494; Mature: 35494

Theoretical pI: Translated: 10.28; Mature: 10.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1a87a548)-;

Cys/Met content:

1.3 %Cys     (Translated Protein)
6.2 %Met     (Translated Protein)
7.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
6.2 %Met     (Mature Protein)
7.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRIGAMARPFLKRRESIAR
CCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDSRVRKWFDVEGLDNLKRAQ
CCHHEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHCCCCHHHHHHHH
MQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQLMEWVQTRGRMRSNKAMIGRN
HHCCCEEEEEEEEEEHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEECCC
NLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVENVATTNGTYVLSRLSGAAMLTVT
HHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCHHEECCCCCCCCCEEEEHHCCCHHHHHH
MVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKIIEKEIMRAPEQYLWIHRRFKTRPVG
HHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCC
ESSLYI
CCCCCH
>Mature Secondary Structure
MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRIGAMARPFLKRRESIAR
CCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDSRVRKWFDVEGLDNLKRAQ
CCHHEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHCCCCHHHHHHHH
MQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQLMEWVQTRGRMRSNKAMIGRN
HHCCCEEEEEEEEEEHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEECCC
NLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVENVATTNGTYVLSRLSGAAMLTVT
HHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCHHEECCCCCCCCCEEEEHHCCCHHHHHH
MVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKIIEKEIMRAPEQYLWIHRRFKTRPVG
HHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCC
ESSLYI
CCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: KDO2-lipid IVA; palmitoleoyl-ACP [C]

Specific reaction: KDO2-lipid IVA + palmitoleoyl-ACP = KDO2-(palmitoleoyl)-lipid IVA + [acyl carrier protein] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503