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The map label for this gene is yiaO [H]

Identifier: 218691869

GI number: 218691869

Start: 4188160

End: 4189146

Strand: Direct

Name: yiaO [H]

Synonym: ECED1_4268

Alternate gene names: 218691869

Gene position: 4188160-4189146 (Clockwise)

Preceding gene: 218691868

Following gene: 218691870

Centisome position: 80.39

GC content: 50.86

Gene sequence:

>987_bases
ATGAAATTACGTTCTGTAACCTACGCTTTATTAATTGCCGGAGTGGCGACGTTCAGCACATCTTCTCTGGCGGCACAATC
ATTACGTTTCGGTTATGAAACCTCACAAACCGATTCACAACATATTGCGGCGAAAAAATTTAATGAATTACTGCAGGAAA
AAACCAAAGGCGAACTGAAATTAAAACTGTTCCCGGATAGCACCCTTGGTAATGCGCAGGCGATGATCAGCGGCGTGCGT
GGCGGCACTATTGATATGGAGATGTCTGGCTCTAACAACTTTACGGGACTCTCGCCCGTCATCAACCTGCTCGATGTCCC
GTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGACCTGAAAGTCTCCCTTGAAG
GTAAGGGGCTGAAAGTACTGGCCTACTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGTGCACCGGTCAAAACGCCT
GCCGACCTGAAAGGGTTGAAAATCCGCACCAACAACAGCCCGATGAATATCGCTGCATTTAAAGTTTTTGGCGCTAACCC
GATCCCGATGCCGTTTGCCGAAGTCTATACCGGGCTGGAAACCCGCACTATCGACGCTCAGGAACACCCGATCAACGTCG
TCTGGTCAGCAAAATTTTATGAAGTGCAGAAGTACCTTTCTCTGACCCATCACGCCTATTCGCCGCTTCTGGTGGTGATC
AACAAAGCGAAGTTTGATGGCTTAACCCCGGAGTTCCAGCAAGCACTGATCTCATCTGCACAGGAAGCGGGTAGCTATCA
GCGCAAACTGGTCGCCGAAGATCAGCAAAAAATCATCGACGGTATGAAAGAAGCGGGCGTGGAAGTCATCACCGATCTCG
ACCGCAAAGCCTTTAGCGATGCGCTGGGTACCCAGGTCCGTGACATGTTTGTTAAAGATGTTCCGCAGGGTGCTGATCTG
CTGAAAGCCGTGGATGAGGTGCAATAA

Upstream 100 bases:

>100_bases
GCCTTACGTCATGGTTTTATTCATGTTGTTAGCATTATTTATTTTTATACCGGAGCTCATTACCGCTCCTCTTAAATGGA
TGAGTTAAAAGGAAATCATA

Downstream 100 bases:

>100_bases
ACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGAGAAGGCCGGGA
GGCGGGCGTGCAGCGCCTGC

Product: transporter

Products: NA

Alternate protein names: 2,3-DKG-binding protein; Extracytoplasmic solute receptor protein yiaO [H]

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MKLRSVTYALLIAGVATFSTSSLAAQSLRFGYETSQTDSQHIAAKKFNELLQEKTKGELKLKLFPDSTLGNAQAMISGVR
GGTIDMEMSGSNNFTGLSPVINLLDVPFLFRDTAHAHKTLDGKVGDDLKVSLEGKGLKVLAYWENGWRDVTNSRAPVKTP
ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAKFYEVQKYLSLTHHAYSPLLVVI
NKAKFDGLTPEFQQALISSAQEAGSYQRKLVAEDQQKIIDGMKEAGVEVITDLDRKAFSDALGTQVRDMFVKDVPQGADL
LKAVDEVQ

Sequences:

>Translated_328_residues
MKLRSVTYALLIAGVATFSTSSLAAQSLRFGYETSQTDSQHIAAKKFNELLQEKTKGELKLKLFPDSTLGNAQAMISGVR
GGTIDMEMSGSNNFTGLSPVINLLDVPFLFRDTAHAHKTLDGKVGDDLKVSLEGKGLKVLAYWENGWRDVTNSRAPVKTP
ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAKFYEVQKYLSLTHHAYSPLLVVI
NKAKFDGLTPEFQQALISSAQEAGSYQRKLVAEDQQKIIDGMKEAGVEVITDLDRKAFSDALGTQVRDMFVKDVPQGADL
LKAVDEVQ
>Mature_328_residues
MKLRSVTYALLIAGVATFSTSSLAAQSLRFGYETSQTDSQHIAAKKFNELLQEKTKGELKLKLFPDSTLGNAQAMISGVR
GGTIDMEMSGSNNFTGLSPVINLLDVPFLFRDTAHAHKTLDGKVGDDLKVSLEGKGLKVLAYWENGWRDVTNSRAPVKTP
ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAKFYEVQKYLSLTHHAYSPLLVVI
NKAKFDGLTPEFQQALISSAQEAGSYQRKLVAEDQQKIIDGMKEAGVEVITDLDRKAFSDALGTQVRDMFVKDVPQGADL
LKAVDEVQ

Specific function: Part of the tripartite ATP-independent periplasmic (TRAP) transport system yiaMNO involved in the uptake of 2,3- diketo-L-gulonate. This protein specifically binds 2,3-diketo-L- gulonate. Is not able to bind either L-ascorbate or dehydroascorbate [H]

COG id: COG1638

COG function: function code G; TRAP-type C4-dicarboxylate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 7 family [H]

Homologues:

Organism=Escherichia coli, GI1790004, Length=328, Percent_Identity=95.7317073170732, Blast_Score=654, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018389
- InterPro:   IPR004682 [H]

Pfam domain/function: PF03480 SBP_bac_7 [H]

EC number: NA

Molecular weight: Translated: 35999; Mature: 35999

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLRSVTYALLIAGVATFSTSSLAAQSLRFGYETSQTDSQHIAAKKFNELLQEKTKGELK
CCCHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEE
LKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFTGLSPVINLLDVPFLFRDTAHAHKTL
EEECCCCCCCCHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHCCCHHHHCHHHHHHCC
DGKVGDDLKVSLEGKGLKVLAYWENGWRDVTNSRAPVKTPADLKGLKIRTNNSPMNIAAF
CCCCCCCEEEEECCCCEEEEEEECCCCHHHCCCCCCCCCCCCCCCEEEEECCCCCEEEEE
KVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAKFYEVQKYLSLTHHAYSPLLVVI
EEECCCCCCCCHHHHHCCCCCEEECCCCCCEEEEECHHHHHHHHHHHHHHHHCCCEEEEE
NKAKFDGLTPEFQQALISSAQEAGSYQRKLVAEDQQKIIDGMKEAGVEVITDLDRKAFSD
ECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
ALGTQVRDMFVKDVPQGADLLKAVDEVQ
HHHHHHHHHHHHCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MKLRSVTYALLIAGVATFSTSSLAAQSLRFGYETSQTDSQHIAAKKFNELLQEKTKGELK
CCCHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEE
LKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFTGLSPVINLLDVPFLFRDTAHAHKTL
EEECCCCCCCCHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHCCCHHHHCHHHHHHCC
DGKVGDDLKVSLEGKGLKVLAYWENGWRDVTNSRAPVKTPADLKGLKIRTNNSPMNIAAF
CCCCCCCEEEEECCCCEEEEEEECCCCHHHCCCCCCCCCCCCCCCEEEEECCCCCEEEEE
KVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAKFYEVQKYLSLTHHAYSPLLVVI
EEECCCCCCCCHHHHHCCCCCEEECCCCCCEEEEECHHHHHHHHHHHHHHHHCCCEEEEE
NKAKFDGLTPEFQQALISSAQEAGSYQRKLVAEDQQKIIDGMKEAGVEVITDLDRKAFSD
ECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
ALGTQVRDMFVKDVPQGADLLKAVDEVQ
HHHHHHHHHHHHCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503 [H]