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The map label for this gene is gph [H]

Identifier: 218550641

GI number: 218550641

Start: 3407966

End: 3408724

Strand: Reverse

Name: gph [H]

Synonym: EFER_3351

Alternate gene names: 218550641

Gene position: 3408724-3407966 (Counterclockwise)

Preceding gene: 218550642

Following gene: 218550640

Centisome position: 74.29

GC content: 51.78

Gene sequence:

>759_bases
ATGAATAAGTTTGAAGATATTCGCGGCGTCGCTTTTGATCTCGATGGTACGCTGGTCGACAGTGCTCCTGGTCTGGCTGC
TGCGGTAGATATGGCGCTGTATGCGCTGGAGTTGCCCGTCGCAGGTGAAGAGCGTGTTATTACCTGGATTGGTAATGGCG
CAGATGTTCTGATGGAGCGTGCATTGACCTGGGCGCGTCAGGAACGTGCGACTCTGCGTAAAACAATGGGTAAACCGCCC
GTTGATGACGACATTCCGGCAGAAGAACAGGTACGTATTCTGCGTAAACTGTTCGATCGCTACTATGGCGAGGTTGCCGA
AGAGGGGACGTTTTTGTTCCCGCACGTTGCCGATACGCTGGGCGCATTGCAGGCTAAAGGCCTGCCGCTAGGTCTGGTTA
CCAACAAACCGACGCCGTTTGTTGCGCCGCTGCTCGAAGCCTTAGATATCGCCAAATATTTCAGCGTGGTGATCGGTGGC
GATGATGTGCAAAACAAAAAGCCGCACCCGGACCCGCTATTACTGGTGGCTGAACGAATGGGCATTGCCCCACAACAGAT
GCTGTTTGTTGGCGACTCACGCAATGATATTCAGGCAGCAAAAGCAGCTGGTTGCCCTTCTGTTGGTTTAACCTATGGAT
ATAACTACGGCGAGGCTATCGATCTTAGCCAGCCTGATGTAATTTATCAGTCTATCAATGACCTTCTGCCCGCATTAGGG
CTTCCGCATAGCGAAAATCAGGAATCGAAAAATGACTAA

Upstream 100 bases:

>100_bases
GCAGGTGCCGATATGTTCGTCGCTGGTTCGGCCATTTTCGATCAGCCAGACTACAAAAAGGTCATTGATGATATGCGCAG
TGAACTGGCAAAGGTAAGTC

Downstream 100 bases:

>100_bases
GCCCATCGTTTTTAGTGGCGCACAGCCCTCAGGTGAATTGACCATTGGTAACTACATGGGTGCGCTGCGTCAGTGGGTAA
ACATGCAGGATGACTACCAT

Product: phosphoglycolate phosphatase

Products: NA

Alternate protein names: PGP; PGPase [H]

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEERVITWIGNGADVLMERALTWARQERATLRKTMGKPP
VDDDIPAEEQVRILRKLFDRYYGEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGEAIDLSQPDVIYQSINDLLPALG
LPHSENQESKND

Sequences:

>Translated_252_residues
MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEERVITWIGNGADVLMERALTWARQERATLRKTMGKPP
VDDDIPAEEQVRILRKLFDRYYGEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGEAIDLSQPDVIYQSINDLLPALG
LPHSENQESKND
>Mature_252_residues
MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEERVITWIGNGADVLMERALTWARQERATLRKTMGKPP
VDDDIPAEEQVRILRKLFDRYYGEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGEAIDLSQPDVIYQSINDLLPALG
LPHSENQESKND

Specific function: Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stres

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=252, Percent_Identity=99.6031746031746, Blast_Score=509, Evalue=1e-146,
Organism=Escherichia coli, GI1787576, Length=211, Percent_Identity=23.696682464455, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833
- InterPro:   IPR006346
- InterPro:   IPR023198 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.1.3.18 [H]

Molecular weight: Translated: 27374; Mature: 27374

Theoretical pI: Translated: 4.32; Mature: 4.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEERVITWIGNGADVLMER
CCCHHHHCCEEEECCCEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHH
ALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYYGEVAEEGTFLFPHVADTL
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEECHHHHHHH
GALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGGDDVQNKKPHPDPLLLVAERM
HHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHH
GIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGEAIDLSQPDVIYQSINDLLPALG
CCCHHHEEEECCCCCHHHHHHHCCCCCCCCEECCCCCCEECCCCCHHHHHHHHHHHHHHC
LPHSENQESKND
CCCCCCCCCCCC
>Mature Secondary Structure
MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEERVITWIGNGADVLMER
CCCHHHHCCEEEECCCEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHH
ALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYYGEVAEEGTFLFPHVADTL
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEECHHHHHHH
GALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGGDDVQNKKPHPDPLLLVAERM
HHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHH
GIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGEAIDLSQPDVIYQSINDLLPALG
CCCHHHEEEECCCCCHHHHHHHCCCCCCCCEECCCCCCEECCCCCHHHHHHHHHHHHHHC
LPHSENQESKND
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]