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The map label for this gene is trpS

Identifier: 218550640

GI number: 218550640

Start: 3406969

End: 3407973

Strand: Reverse

Name: trpS

Synonym: EFER_3350

Alternate gene names: 218550640

Gene position: 3407973-3406969 (Counterclockwise)

Preceding gene: 218550641

Following gene: 218550639

Centisome position: 74.27

GC content: 52.24

Gene sequence:

>1005_bases
ATGACTAAGCCCATCGTTTTTAGTGGCGCACAGCCCTCAGGTGAATTGACCATTGGTAACTACATGGGTGCGCTGCGTCA
GTGGGTAAACATGCAGGATGACTACCATTGCATTTACTGTATCGTTGACCAACACGCGATCACCGTGCGCCAGGATGCAC
AGAAGCTGCGTAAAGCGACGCTGGATACGCTGGCCTTGTATCTGGCTTGTGGTATCGATCCTGAGAAAAGCACCATTTTT
GTTCAGTCCCACGTGCCGGAACATGCGCAGTTAGGCTGGGCACTGAACTGCTATACCTACTTCGGCGAACTGAGCCGCAT
GACGCAGTTTAAAGATAAATCTGCGCGTTATGCCGAGAACATCAACGCGGGTCTGTTTGACTATCCGGTGCTGATGGCGG
CGGATATCCTACTGTATCAAACCAACCTGGTGCCGGTGGGTGAAGACCAGAAACAGCACCTGGAACTGAGCCGCGATATC
GCCCAGCGTTTCAACGCGCTGTATGGCGATATTTTTAAAGTACCGGAGCCGTTTATTCCGAAATCTGGCGCGCGCGTAAT
GTCGCTGCTGGAGCCGACCAAGAAGATGTCCAAGTCTGACGATAACCGCAATAACGTTATCGGCCTGCTGGAAGATCCGA
AATCGGTAGTGAAGAAAATCAAACGCGCGGTCACTGACTCCGACGAGCCGCCGGTAGTTCGCTACGATGTGCAGAATAAA
GCGGGCGTTTCCAACCTGCTGGATATTCTTTCTGCGGTAACTGGCCAGAGTATCCCGGAACTGGAAAAACAGTTCGAAGG
CAAGATGTATGGTCATCTGAAAGGCGAAGTGGCTGATGCCGTTTCCGGTATGCTGACTGAATTGCAGGAACGCTATCACC
GTTTCCGCAATGACGAAGCCTTCCTGCAACAGGTGATGAAAGATGGCGCAGAAAAAGCCAGCGCACACGCTTCCCGTACG
CTAAAAGCGGTGTACGAAGCGATTGGATTCGTGGCGAAGCCGTAA

Upstream 100 bases:

>100_bases
GAGGCTATCGATCTTAGCCAGCCTGATGTAATTTATCAGTCTATCAATGACCTTCTGCCCGCATTAGGGCTTCCGCATAG
CGAAAATCAGGAATCGAAAA

Downstream 100 bases:

>100_bases
TTTAACATTAATGCCAAAAACCGGGGAAACCCGGTTTTTTTATCCTCTTTTTGCAATAATCCGAAAAAATGTGAAGCGCC
TCGCCGTTTCCATATCTCAT

Product: tryptophanyl-tRNA synthetase

Products: NA

Alternate protein names: Tryptophan--tRNA ligase; TrpRS

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQHAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIF
VQSHVPEHAQLGWALNCYTYFGELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
AQRFNALYGDIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVKKIKRAVTDSDEPPVVRYDVQNK
AGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGEVADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASAHASRT
LKAVYEAIGFVAKP

Sequences:

>Translated_334_residues
MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQHAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIF
VQSHVPEHAQLGWALNCYTYFGELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
AQRFNALYGDIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVKKIKRAVTDSDEPPVVRYDVQNK
AGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGEVADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASAHASRT
LKAVYEAIGFVAKP
>Mature_333_residues
TKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQHAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFV
QSHVPEHAQLGWALNCYTYFGELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDIA
QRFNALYGDIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVKKIKRAVTDSDEPPVVRYDVQNKA
GVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGEVADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASAHASRTL
KAVYEAIGFVAKP

Specific function: Unknown

COG id: COG0180

COG function: function code J; Tryptophanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI7710154, Length=331, Percent_Identity=42.9003021148036, Blast_Score=269, Evalue=2e-72,
Organism=Homo sapiens, GI41352700, Length=179, Percent_Identity=51.9553072625698, Blast_Score=194, Evalue=8e-50,
Organism=Escherichia coli, GI1789786, Length=334, Percent_Identity=99.7005988023952, Blast_Score=697, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71982800, Length=334, Percent_Identity=35.9281437125749, Blast_Score=208, Evalue=3e-54,
Organism=Caenorhabditis elegans, GI71982793, Length=334, Percent_Identity=35.9281437125749, Blast_Score=206, Evalue=9e-54,
Organism=Saccharomyces cerevisiae, GI6320474, Length=345, Percent_Identity=38.5507246376812, Blast_Score=226, Evalue=3e-60,
Organism=Drosophila melanogaster, GI24666151, Length=334, Percent_Identity=43.4131736526946, Blast_Score=247, Evalue=9e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYW_ECO57 (P67589)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   B91157
- PIR:   H86002
- RefSeq:   NP_289923.1
- RefSeq:   NP_312253.1
- ProteinModelPortal:   P67589
- SMR:   P67589
- EnsemblBacteria:   EBESCT00000024996
- EnsemblBacteria:   EBESCT00000055707
- GeneID:   915919
- GeneID:   958834
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z4737
- KEGG:   ecs:ECs4226
- GeneTree:   EBGT00050000010970
- HOGENOM:   HBG293263
- OMA:   NKPGVSN
- ProtClustDB:   PRK00927
- BioCyc:   ECOL83334:ECS4226-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00140_B
- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002306
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10055
- PRINTS:   PR01039
- TIGRFAMs:   TIGR00233

Pfam domain/function: PF00579 tRNA-synt_1b

EC number: =6.1.1.2

Molecular weight: Translated: 37424; Mature: 37293

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 198-198

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQHAITVRQDAQKLRKAT
CCCCEEEECCCCCCCEEHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHHH
LDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFGELSRMTQFKDKSARYAEN
HHHHHHHHHHCCCCCCCEEEEECCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
INAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDIAQRFNALYGDIFKVPEPFIP
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
KSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVKKIKRAVTDSDEPPVVRYDVQNK
CCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCCCEEEEECCCC
AGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGEVADAVSGMLTELQERYHRFRNDEA
CCHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
FLQQVMKDGAEKASAHASRTLKAVYEAIGFVAKP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQHAITVRQDAQKLRKAT
CCCEEEECCCCCCCEEHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHHH
LDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFGELSRMTQFKDKSARYAEN
HHHHHHHHHHCCCCCCCEEEEECCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
INAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDIAQRFNALYGDIFKVPEPFIP
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
KSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVKKIKRAVTDSDEPPVVRYDVQNK
CCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCCCEEEEECCCC
AGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGEVADAVSGMLTELQERYHRFRNDEA
CCHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
FLQQVMKDGAEKASAHASRTLKAVYEAIGFVAKP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796