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The map label for this gene is cusR

Identifier: 218549855

GI number: 218549855

Start: 2603799

End: 2604482

Strand: Direct

Name: cusR

Synonym: EFER_2540

Alternate gene names: 218549855

Gene position: 2603799-2604482 (Clockwise)

Preceding gene: 218549848

Following gene: 218549856

Centisome position: 56.74

GC content: 53.36

Gene sequence:

>684_bases
ATGAAACTGTTGATTGTCGAAGATGAAAAGAAAACCGGCGAATACTTAACCAAAGGGTTAACCGAGGCCGGTTTTGTGGT
TGATTTGGCCGATAACGGGCTGAATGGATATCATCTGGCGATGACAGGTGATTATGACCTGATAATCCTCGATATTATGC
TGCCGGACGTGAACGGCTGGGATATTGTGCGCATGTTGCGTTCCGCCAATAAAGGGATGCCGATTCTGTTGCTTACCGCG
CTTGGCACCATTGAACATCGCGTCAAGGGGCTGGAGCTGGGAGCGGATGACTATCTGGTGAAGCCGTTCGCTTTTGCTGA
ACTGCTGGCGCGGGTGCGCACTCTACTGCGTCGCGGGGCGGCGGTGATTATCGAAAGTCAGTTTCAGGTTGCCGACCTGA
TGGTCGATCTCGTCAGCCGCAAAGTCACCCGCAGCGGCACGCGCATCACTTTGACCAGTAAAGAGTTTACTCTGCTGGAG
TTTTTTCTCCGCCATCAGGGCGAAGTGCTGCCCCGCTCGCTTATCGCCTCGCAGGTATGGGACATGAATTTTGACAGCGA
CACTAACGCCATTGATGTGGCGGTGAAGCGACTGCGCGGCAAAATCGACAACGACTTTGAGCCAAAGCTAATTCAGACCG
TACGCGGCGTAGGCTATATGCTTGAGGTGCCGGATGGCCAGTAA

Upstream 100 bases:

>100_bases
TTCCGGTGACAGGAAAATGACAAAATTGTCATTTTGCCAATAAGCGATTGCCATCTGATCCCGCTACTCTAGAATTGCCC
GGGCAACATGCGGAGGAAAT

Downstream 100 bases:

>100_bases
GCCGTTTCAGCGCCCGTTTTCGCTGGCAACCCGCCTGACCTTTTTTATCAGCCTTGCCACCATTGCAGCGTTTTTCGTCT
TTGCCTGGATCATGATCCAC

Product: DNA-binding transcriptional activator CusR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 227; Mature: 227

Protein sequence:

>227_residues
MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGDYDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTA
LGTIEHRVKGLELGADDYLVKPFAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVGYMLEVPDGQ

Sequences:

>Translated_227_residues
MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGDYDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTA
LGTIEHRVKGLELGADDYLVKPFAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVGYMLEVPDGQ
>Mature_227_residues
MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGDYDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTA
LGTIEHRVKGLELGADDYLVKPFAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVGYMLEVPDGQ

Specific function: Member of the two-component regulatory system CusS/CusR. Activates the expression of cusCFBA in response to increasing levels of copper ions, and probably silver ions

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain

Homologues:

Organism=Escherichia coli, GI1786784, Length=227, Percent_Identity=100, Blast_Score=456, Evalue=1e-130,
Organism=Escherichia coli, GI87082012, Length=219, Percent_Identity=51.5981735159817, Blast_Score=237, Evalue=5e-64,
Organism=Escherichia coli, GI1789402, Length=223, Percent_Identity=39.9103139013453, Blast_Score=157, Evalue=4e-40,
Organism=Escherichia coli, GI1789809, Length=232, Percent_Identity=37.5, Blast_Score=139, Evalue=1e-34,
Organism=Escherichia coli, GI1786599, Length=220, Percent_Identity=34.0909090909091, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1790552, Length=221, Percent_Identity=35.2941176470588, Blast_Score=130, Evalue=7e-32,
Organism=Escherichia coli, GI1790860, Length=221, Percent_Identity=35.7466063348416, Blast_Score=127, Evalue=8e-31,
Organism=Escherichia coli, GI1787375, Length=224, Percent_Identity=33.4821428571429, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI1786911, Length=220, Percent_Identity=35.4545454545455, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1788394, Length=223, Percent_Identity=34.0807174887892, Blast_Score=111, Evalue=3e-26,
Organism=Escherichia coli, GI1787229, Length=227, Percent_Identity=32.15859030837, Blast_Score=104, Evalue=5e-24,
Organism=Escherichia coli, GI2367329, Length=229, Percent_Identity=34.061135371179, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI145693140, Length=231, Percent_Identity=30.7359307359307, Blast_Score=98, Evalue=4e-22,
Organism=Escherichia coli, GI1790863, Length=226, Percent_Identity=28.7610619469027, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI1788550, Length=107, Percent_Identity=33.6448598130841, Blast_Score=74, Evalue=6e-15,
Organism=Escherichia coli, GI1788713, Length=115, Percent_Identity=32.1739130434783, Blast_Score=63, Evalue=1e-11,
Organism=Escherichia coli, GI1790437, Length=124, Percent_Identity=27.4193548387097, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CUSR_ECO57 (P0AD00)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   A90705
- PIR:   D85555
- RefSeq:   NP_286296.1
- RefSeq:   NP_308636.1
- ProteinModelPortal:   P0AD00
- SMR:   P0AD00
- EnsemblBacteria:   EBESCT00000028107
- EnsemblBacteria:   EBESCT00000055610
- GeneID:   916967
- GeneID:   957567
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z0709
- KEGG:   ecs:ECs0609
- GeneTree:   EBGT00070000031686
- HOGENOM:   HBG753323
- OMA:   AVEDRVH
- ProtClustDB:   PRK09836
- BioCyc:   ECOL83334:ECS0609-MONOMER
- GO:   GO:0005737
- GO:   GO:0006350
- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR006291
- InterPro:   IPR001789
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- SMART:   SM00448
- SMART:   SM00862
- TIGRFAMs:   TIGR01387

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C; SSF52172 CheY_like

EC number: NA

Molecular weight: Translated: 25395; Mature: 25395

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGDYDLIILDIMLPDVNGW
CEEEEEECCCHHHHHHHHCCCCCCEEEEECCCCCCCEEEEEECCCCEEEEEEEECCCCCH
DIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKPFAFAELLARVRTLLRRGA
HHHHHHHHCCCCCCEEEEHHHHHHHHHHHCCEECCCCCEECHHHHHHHHHHHHHHHHCCC
AVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLEFFLRHQGEVLPRSLIASQVW
EEEEECCHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHCCCCCHHHHHHHHHH
DMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVGYMLEVPDGQ
CCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCEEEECCCCC
>Mature Secondary Structure
MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGDYDLIILDIMLPDVNGW
CEEEEEECCCHHHHHHHHCCCCCCEEEEECCCCCCCEEEEEECCCCEEEEEEEECCCCCH
DIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKPFAFAELLARVRTLLRRGA
HHHHHHHHCCCCCCEEEEHHHHHHHHHHHCCEECCCCCEECHHHHHHHHHHHHHHHHCCC
AVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLEFFLRHQGEVLPRSLIASQVW
EEEEECCHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHCCCCCHHHHHHHHHH
DMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVGYMLEVPDGQ
CCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796