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The map label for this gene is cusS [H]

Identifier: 218549856

GI number: 218549856

Start: 2604460

End: 2605920

Strand: Direct

Name: cusS [H]

Synonym: EFER_2541

Alternate gene names: 218549856

Gene position: 2604460-2605920 (Clockwise)

Preceding gene: 218549855

Following gene: 218549857

Centisome position: 56.76

GC content: 52.29

Gene sequence:

>1461_bases
TTGAGGTGCCGGATGGCCAGTAAGCCGTTTCAGCGCCCGTTTTCGCTGGCAACCCGCCTGACCTTTTTTATCAGCCTTGC
CACCATTGCAGCGTTTTTCGTCTTTGCCTGGATCATGATCCACTCGGTAAAAGTGCATTTTGCCGAGCAGGATATTAATG
ATTTAAAAGAGATTAGCGCCACATTCAAGCGCGTTCTTAATCATCCCGAAGAGACACAAGCCCGACGCTTAATGACGCTG
GAAGATATCGTCAGTGGTTATTCCAACGTGTTGATTTCTCTGGCAGATAGCCACGGTAAAACGGTGTATCACTCCCCCGG
TGCGCCGGATATCCGCGAGTTTGCACGTGATGCCATACCCGATAAAGACGCTCGGGGCGGCGAGGTGTATCTCCTTTCCG
GCCCGACAATGATGATGCCAGGCCACGGTCACGGGCATATGGAACACAGCAACTGGCGGATGATTAACTTGCCGGTTGGC
CCGTTGGTGGACGGCAAACCGATTTATACGCTCTACATCGCGCTGTCTATCGATTTTCATCTTCATTACATTAATGATTT
GATGAATAAACTTATTATGACCGCATCGATCATCAGCATCCTGATCGTCTTTATCGTGCTGCTGGCGGTACATAAAGGCC
ACGCGCCAATTCGCAGCGTCAGCCGTCAAATCCAGAACATTACCTCGAAAGATCTCGACGTTCGCCTCGATCCACAGACC
GTGCCCATTGAACTCGAACAATTGGGGCTGTCGTTCAACCATATGATCGAGCGTATCGAGGATGTCTTTACCCGCCAGTC
CAATTTCTCAGCGGATATCGCCCACGAAATTCGCACGCCGATTACGAATCTCATAACGCAAACGGAAATCGCCCTCAGCC
AGTCGCGCAGCCAGAAGGAGCTGGAAGATGTGCTCTACTCTAATCTCGAAGAGCTGACGCGAATGGCGAAAATGGTCAGC
GATATGCTGTTTCTCGCTCAGGCCGATAACAACCAGTTAATCCCCGAAAAGAAAATGCTCAACCTGGCGGATGAAGTTGG
CAAAGTGTTCGATTTTTTCGAGGCGTTAGCGGAAGATCGCGGCGTAGCACTACGGTTTGTTGGCGACAAATGTCAGGTTG
CGGGCGATCCGCTGATGCTGCGTCGGGCGTTAAGCAACCTGCTTTCTAACGCCCTGCGTTATACGCCACCCGGAAAGGCA
ATTGTAGTGCGCTGCCAGACGGTCGATCACCAGGTGCAAGTTACCGTCGAAAACCCCGGTGCTCCCATTGCGCCCGAGCA
CTTACCGCGATTGTTTGACCGTTTCTACCGCGTTGACCCTTCCCGCCAGCGAAAAGGTGAAGGCAGCGGCATTGGGCTGG
CGATAGTGAAATCGATTGTTGTCGCGCATAAAGGCACGGTTGCAGTAACGTCAGATGCGCGGGGAACAAGGTTTGTTATC
GGGCTACCGGAGAGAGAGTGA

Upstream 100 bases:

>100_bases
ACTAACGCCATTGATGTGGCGGTGAAGCGACTGCGCGGCAAAATCGACAACGACTTTGAGCCAAAGCTAATTCAGACCGT
ACGCGGCGTAGGCTATATGC

Downstream 100 bases:

>100_bases
TTGGTCAGATAATGCAGTGGATGTTAATTGAATTGAGAATTAGCTATTTCGTCACGAGAAAAAGCGTAAAATCCGTTTCG
TGCAGATGGTGAGTTTTATC

Product: sensor kinase CusS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 486; Mature: 486

Protein sequence:

>486_residues
MRCRMASKPFQRPFSLATRLTFFISLATIAAFFVFAWIMIHSVKVHFAEQDINDLKEISATFKRVLNHPEETQARRLMTL
EDIVSGYSNVLISLADSHGKTVYHSPGAPDIREFARDAIPDKDARGGEVYLLSGPTMMMPGHGHGHMEHSNWRMINLPVG
PLVDGKPIYTLYIALSIDFHLHYINDLMNKLIMTASIISILIVFIVLLAVHKGHAPIRSVSRQIQNITSKDLDVRLDPQT
VPIELEQLGLSFNHMIERIEDVFTRQSNFSADIAHEIRTPITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVS
DMLFLAQADNNQLIPEKKMLNLADEVGKVFDFFEALAEDRGVALRFVGDKCQVAGDPLMLRRALSNLLSNALRYTPPGKA
IVVRCQTVDHQVQVTVENPGAPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLAIVKSIVVAHKGTVAVTSDARGTRFVI
GLPERE

Sequences:

>Translated_486_residues
MRCRMASKPFQRPFSLATRLTFFISLATIAAFFVFAWIMIHSVKVHFAEQDINDLKEISATFKRVLNHPEETQARRLMTL
EDIVSGYSNVLISLADSHGKTVYHSPGAPDIREFARDAIPDKDARGGEVYLLSGPTMMMPGHGHGHMEHSNWRMINLPVG
PLVDGKPIYTLYIALSIDFHLHYINDLMNKLIMTASIISILIVFIVLLAVHKGHAPIRSVSRQIQNITSKDLDVRLDPQT
VPIELEQLGLSFNHMIERIEDVFTRQSNFSADIAHEIRTPITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVS
DMLFLAQADNNQLIPEKKMLNLADEVGKVFDFFEALAEDRGVALRFVGDKCQVAGDPLMLRRALSNLLSNALRYTPPGKA
IVVRCQTVDHQVQVTVENPGAPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLAIVKSIVVAHKGTVAVTSDARGTRFVI
GLPERE
>Mature_486_residues
MRCRMASKPFQRPFSLATRLTFFISLATIAAFFVFAWIMIHSVKVHFAEQDINDLKEISATFKRVLNHPEETQARRLMTL
EDIVSGYSNVLISLADSHGKTVYHSPGAPDIREFARDAIPDKDARGGEVYLLSGPTMMMPGHGHGHMEHSNWRMINLPVG
PLVDGKPIYTLYIALSIDFHLHYINDLMNKLIMTASIISILIVFIVLLAVHKGHAPIRSVSRQIQNITSKDLDVRLDPQT
VPIELEQLGLSFNHMIERIEDVFTRQSNFSADIAHEIRTPITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVS
DMLFLAQADNNQLIPEKKMLNLADEVGKVFDFFEALAEDRGVALRFVGDKCQVAGDPLMLRRALSNLLSNALRYTPPGKA
IVVRCQTVDHQVQVTVENPGAPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLAIVKSIVVAHKGTVAVTSDARGTRFVI
GLPERE

Specific function: Member of the two-component regulatory system CusS/CusR. Copper ion sensor. Could also be a silver ion sensor. Probably activates CusR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1786783, Length=479, Percent_Identity=96.2421711899791, Blast_Score=935, Evalue=0.0,
Organism=Escherichia coli, GI1788279, Length=296, Percent_Identity=33.1081081081081, Blast_Score=155, Evalue=6e-39,
Organism=Escherichia coli, GI1786600, Length=265, Percent_Identity=34.7169811320755, Blast_Score=135, Evalue=5e-33,
Organism=Escherichia coli, GI1790346, Length=332, Percent_Identity=29.8192771084337, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1788393, Length=275, Percent_Identity=30.9090909090909, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI1787894, Length=311, Percent_Identity=29.5819935691318, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI145693157, Length=237, Percent_Identity=27.0042194092827, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI1789403, Length=282, Percent_Identity=30.4964539007092, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1790551, Length=296, Percent_Identity=27.7027027027027, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1789808, Length=285, Percent_Identity=27.719298245614, Blast_Score=82, Evalue=9e-17,
Organism=Escherichia coli, GI1789149, Length=267, Percent_Identity=26.9662921348315, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1788713, Length=230, Percent_Identity=29.1304347826087, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI87081816, Length=253, Percent_Identity=27.2727272727273, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1790436, Length=220, Percent_Identity=25.9090909090909, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI87082128, Length=295, Percent_Identity=24.0677966101695, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1790861, Length=295, Percent_Identity=25.4237288135593, Blast_Score=70, Evalue=5e-13,
Organism=Escherichia coli, GI48994928, Length=236, Percent_Identity=25.8474576271186, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1786912, Length=217, Percent_Identity=25.8064516129032, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1787374, Length=340, Percent_Identity=21.1764705882353, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1788549, Length=223, Percent_Identity=26.457399103139, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR006290
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 54491; Mature: 54491

Theoretical pI: Translated: 7.35; Mature: 7.35

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRCRMASKPFQRPFSLATRLTFFISLATIAAFFVFAWIMIHSVKVHFAEQDINDLKEISA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHH
TFKRVLNHPEETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSPGAPDIREFARDAIP
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEECCCCCHHHHHHHHCCC
DKDARGGEVYLLSGPTMMMPGHGHGHMEHSNWRMINLPVGPLVDGKPIYTLYIALSIDFH
CCCCCCCEEEEEECCEEECCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEEEEEEEHH
LHYINDLMNKLIMTASIISILIVFIVLLAVHKGHAPIRSVSRQIQNITSKDLDVRLDPQT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEECCCC
VPIELEQLGLSFNHMIERIEDVFTRQSNFSADIAHEIRTPITNLITQTEIALSQSRSQKE
CCEEHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
LEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKKMLNLADEVGKVFDFFEALAEDR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
GVALRFVGDKCQVAGDPLMLRRALSNLLSNALRYTPPGKAIVVRCQTVDHQVQVTVENPG
CEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCEEEEEEECCC
APIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLAIVKSIVVAHKGTVAVTSDARGTRFVI
CCCCHHHHHHHHHHHHCCCCCHHCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCEEEE
GLPERE
ECCCCC
>Mature Secondary Structure
MRCRMASKPFQRPFSLATRLTFFISLATIAAFFVFAWIMIHSVKVHFAEQDINDLKEISA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHH
TFKRVLNHPEETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSPGAPDIREFARDAIP
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEECCCCCHHHHHHHHCCC
DKDARGGEVYLLSGPTMMMPGHGHGHMEHSNWRMINLPVGPLVDGKPIYTLYIALSIDFH
CCCCCCCEEEEEECCEEECCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEEEEEEEHH
LHYINDLMNKLIMTASIISILIVFIVLLAVHKGHAPIRSVSRQIQNITSKDLDVRLDPQT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEECCCC
VPIELEQLGLSFNHMIERIEDVFTRQSNFSADIAHEIRTPITNLITQTEIALSQSRSQKE
CCEEHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
LEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKKMLNLADEVGKVFDFFEALAEDR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
GVALRFVGDKCQVAGDPLMLRRALSNLLSNALRYTPPGKAIVVRCQTVDHQVQVTVENPG
CEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCEEEEEEECCC
APIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLAIVKSIVVAHKGTVAVTSDARGTRFVI
CCCCHHHHHHHHHHHHCCCCCHHCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCEEEE
GLPERE
ECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]