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The map label for this gene is vsr [H]

Identifier: 218549284

GI number: 218549284

Start: 1992853

End: 1993326

Strand: Reverse

Name: vsr [H]

Synonym: EFER_1944

Alternate gene names: 218549284

Gene position: 1993326-1992853 (Counterclockwise)

Preceding gene: 218549285

Following gene: 218549282

Centisome position: 43.44

GC content: 53.16

Gene sequence:

>474_bases
TTGCAAATGGCCGATGTTCACGATAAAGCCACACGTAGCAAAAACATGCGGGCCATCGCGACCCGCGACACCGCCATCGA
AAAGCGTCTTGCCAGTGTGCTTAACGAGCTTGGTATACCGTATCGCGTTCAGGACGCCTCGCTGCCAGGCAAACCTGATT
TTATTATTGATGATTATCAGTGCGTTATATTTACCCATGGCTGTTTCTGGCACCAGCATCATTGTCACCTGTTTAAAGTT
CCGGCGACACGCACGGCGTTCTGGCTGGAAAAGATTGGCAAAAATGTTGAGCGTGATCGTCGTGACCAGGTGCGTTTGCA
GGCGCTTGGCTGGCGAGTGTTGATCGTCTGGGAGTGTGCGTTGCGGGGGCGAGAAAAACTCAGTGACGCCGCGTTATCTG
AGCGCTTAGAAGAGTGGATCTGCGGCGCTGACGCCGACGCGCAGATCGACACTCTCGGTATTCAATTACTCTGA

Upstream 100 bases:

>100_bases
GTTTATGGCGAAGTTGAGTGCGGAATTAAACGGCGACTATGATTCGTTGGATCAAAACGTTATCTGCACATTTACTCGAT
AAATCATCAGCATAAGGACG

Downstream 100 bases:

>100_bases
ATGTTTGCCCGTGGGAGCACAAACCGCAGCGGATTTATCCGGTAACAGATATTTTCCCAGCGTCACCAGCAACACGGCAA
ACACAATTACCCCCAGTGCC

Product: DNA mismatch endonuclease of very short patch repair

Products: DNA [C]

Alternate protein names: DNA mismatch endonuclease; V.EcoKDcm; Vsr mismatch endonuclease [H]

Number of amino acids: Translated: 157; Mature: 157

Protein sequence:

>157_residues
MQMADVHDKATRSKNMRAIATRDTAIEKRLASVLNELGIPYRVQDASLPGKPDFIIDDYQCVIFTHGCFWHQHHCHLFKV
PATRTAFWLEKIGKNVERDRRDQVRLQALGWRVLIVWECALRGREKLSDAALSERLEEWICGADADAQIDTLGIQLL

Sequences:

>Translated_157_residues
MQMADVHDKATRSKNMRAIATRDTAIEKRLASVLNELGIPYRVQDASLPGKPDFIIDDYQCVIFTHGCFWHQHHCHLFKV
PATRTAFWLEKIGKNVERDRRDQVRLQALGWRVLIVWECALRGREKLSDAALSERLEEWICGADADAQIDTLGIQLL
>Mature_157_residues
MQMADVHDKATRSKNMRAIATRDTAIEKRLASVLNELGIPYRVQDASLPGKPDFIIDDYQCVIFTHGCFWHQHHCHLFKV
PATRTAFWLEKIGKNVERDRRDQVRLQALGWRVLIVWECALRGREKLSDAALSERLEEWICGADADAQIDTLGIQLL

Specific function: Deamination of 5-methylcytosine in DNA results in T/G mismatches. If unrepaired, these mismatches can lead to C-to-C transition mutations. The very short patch (VSP) repair process in E.coli counteracts the mutagenic process by repairing the mismatches in

COG id: COG3727

COG function: function code L; DNA G:T-mismatch repair endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vsr family [H]

Homologues:

Organism=Escherichia coli, GI1788270, Length=155, Percent_Identity=83.2258064516129, Blast_Score=274, Evalue=2e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004603
- InterPro:   IPR011335 [H]

Pfam domain/function: PF03852 Vsr [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 18054; Mature: 18054

Theoretical pI: Translated: 7.51; Mature: 7.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQMADVHDKATRSKNMRAIATRDTAIEKRLASVLNELGIPYRVQDASLPGKPDFIIDDYQ
CCCCCCHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEECCEE
CVIFTHGCFWHQHHCHLFKVPATRTAFWLEKIGKNVERDRRDQVRLQALGWRVLIVWECA
EEEEECCCEEECCCCEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCEEEEEEEEHH
LRGREKLSDAALSERLEEWICGADADAQIDTLGIQLL
HCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEC
>Mature Secondary Structure
MQMADVHDKATRSKNMRAIATRDTAIEKRLASVLNELGIPYRVQDASLPGKPDFIIDDYQ
CCCCCCHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEECCEE
CVIFTHGCFWHQHHCHLFKVPATRTAFWLEKIGKNVERDRRDQVRLQALGWRVLIVWECA
EEEEECCCEEECCCCEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCEEEEEEEEHH
LRGREKLSDAALSERLEEWICGADADAQIDTLGIQLL
HCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: DNA mismatch endonuclease vsr specifically recognizes a T-G mismatch in a special context related to the dcm methylation sequence and makes an incision 5' to the mismatched T residue [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2527357; 2198248; 9097040; 9278503; 1944537; 10871403; 10612397; 10360178 [H]