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The map label for this gene is pgaC [H]

Identifier: 218549246

GI number: 218549246

Start: 1956269

End: 1957594

Strand: Direct

Name: pgaC [H]

Synonym: EFER_1906

Alternate gene names: 218549246

Gene position: 1956269-1957594 (Clockwise)

Preceding gene: 218549245

Following gene: 218549247

Centisome position: 42.63

GC content: 46.83

Gene sequence:

>1326_bases
ATGACTAATCGCATCGTATCGTTTTTTATATTGTGTCTGGTGTTATTTATTCCTCTGAGTGTTGCGTTCTTCCACTCTGG
CGAATTAATGATGAGATTCGTGTTCTTCTGGCCATTTTTTATGTCCATTATGTGGATCGTTGGTGGTGTCTATTTCTGGT
TTCACCGCGAACGCCACTGGCAATGGGGGGAAAATGCACCAGCACCGCAACTTAAGGATAATCCACCGATCTCAATTATC
ATTCCCTGCTTTAATGAAGAGAAAAATGTTGAGGAAACTATTCATGCGGCTTTAGCGCAGCGATATGAAAATATTGAAGT
GATTGCCGTTAATGACGGTTCATCAGATCAAACTCAGGCGATACTGGATCGGCTGGCCTCGCTACACCCCAAACTGCGCG
TCATTCACCTTGCGCAAAACCAGGGTAAAGCGATTGCCCTGAAAACCGGTGCAGCAGCGGCAAGAAGTGAGTATTTAGTC
TGTATTGATGGCGACGCCTTGCTGGATCGCGATGCGGCAGCCTATATAGTGGAGCCCATGCTATACAATCCGCGCGTCGG
CGCGGTGACCGGAAACCCGCGTATTCGCACACGATCCACGCTGGTTGGCAAAGTTCAGGTTGGCGAGTTCTCATCAATTA
TTGGTCTGATCAAACGTACCCAGCGCGTCTACGGTAACGTTTTTACCGTTTCCGGTGTTATCGCCGCATTTCGTCGCAGT
GCACTGGCAGAAGTTGGTTACTGGAGTGACGATATGATCACCGAAGATATTGATATTAGCTGGAAGCTGCAACTGAATCA
GTGGACAATTTTTTACGAACCACGAGCGCTGTGCTGGATATTAATGCCGGAAACGTTAAAAGGGCTGTGGAAACAACGGT
TGCGTTGGGCGCAGGGGGGCGCGGAAGTCTTCCTGAAAAATTTAAATAAATTGTGGCAATGGAAAAATCATCGGATGTGG
CCGCTATTTTTTGAATATTGTCTGACCACCACCTGGGCTTTTACCTGTTTGATTGGTTTGTTCATCTTTATTGCCCAACA
ACTTGGCGTACCTGTAAATGTTGAGCTTAGCCATCTTGCCGCGACTCACACTGCCGGGATATTGCTATGCACATTATGTC
TGCTGCAATTTTTAGTGAGTTTGATTATTGAAAACCGCTATGAACAAAATATGGCTTCATCATTGTTCTGGATTATCTGG
TTCCCGGTCGTCTTCTGGACGCTTAGTCTGGCAACGACTCTGGTGTCTTTTACCCGGGTTATGCTGGTGCGTAAAAAGAA
ACGCGCCCGTTGGATAAGCCCGGATCGTGGAATAATGCGAGGTTAA

Upstream 100 bases:

>100_bases
GGAGTGAAAAACTATGGTTATTACCCTGATGATTTTCTGCGCAATCAGCCTGAAATAAAGGTCATTCGTCCTCAGTTCTC
CACAGCCTGGTATCCAAAAA

Downstream 100 bases:

>100_bases
TATGAATAATTTGATTATTACCACTAAGCGCAGCCCGTTACGGAAATTATTTGATTATCTGGCGACCTTTGTATTATGGA
TTCTGTTCGCATTTTTTATC

Product: N-glycosyltransferase

Products: NA

Alternate protein names: PGA synthase; Poly-beta-1,6-GlcNAc synthase; Biofilm PGA synthesis protein PgaC; N-acetylglucosaminyltransferase PgaC [H]

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MTNRIVSFFILCLVLFIPLSVAFFHSGELMMRFVFFWPFFMSIMWIVGGVYFWFHRERHWQWGENAPAPQLKDNPPISII
IPCFNEEKNVEETIHAALAQRYENIEVIAVNDGSSDQTQAILDRLASLHPKLRVIHLAQNQGKAIALKTGAAAARSEYLV
CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKVQVGEFSSIIGLIKRTQRVYGNVFTVSGVIAAFRRS
ALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRALCWILMPETLKGLWKQRLRWAQGGAEVFLKNLNKLWQWKNHRMW
PLFFEYCLTTTWAFTCLIGLFIFIAQQLGVPVNVELSHLAATHTAGILLCTLCLLQFLVSLIIENRYEQNMASSLFWIIW
FPVVFWTLSLATTLVSFTRVMLVRKKKRARWISPDRGIMRG

Sequences:

>Translated_441_residues
MTNRIVSFFILCLVLFIPLSVAFFHSGELMMRFVFFWPFFMSIMWIVGGVYFWFHRERHWQWGENAPAPQLKDNPPISII
IPCFNEEKNVEETIHAALAQRYENIEVIAVNDGSSDQTQAILDRLASLHPKLRVIHLAQNQGKAIALKTGAAAARSEYLV
CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKVQVGEFSSIIGLIKRTQRVYGNVFTVSGVIAAFRRS
ALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRALCWILMPETLKGLWKQRLRWAQGGAEVFLKNLNKLWQWKNHRMW
PLFFEYCLTTTWAFTCLIGLFIFIAQQLGVPVNVELSHLAATHTAGILLCTLCLLQFLVSLIIENRYEQNMASSLFWIIW
FPVVFWTLSLATTLVSFTRVMLVRKKKRARWISPDRGIMRG
>Mature_440_residues
TNRIVSFFILCLVLFIPLSVAFFHSGELMMRFVFFWPFFMSIMWIVGGVYFWFHRERHWQWGENAPAPQLKDNPPISIII
PCFNEEKNVEETIHAALAQRYENIEVIAVNDGSSDQTQAILDRLASLHPKLRVIHLAQNQGKAIALKTGAAAARSEYLVC
IDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKVQVGEFSSIIGLIKRTQRVYGNVFTVSGVIAAFRRSA
LAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRALCWILMPETLKGLWKQRLRWAQGGAEVFLKNLNKLWQWKNHRMWP
LFFEYCLTTTWAFTCLIGLFIFIAQQLGVPVNVELSHLAATHTAGILLCTLCLLQFLVSLIIENRYEQNMASSLFWIIWF
PVVFWTLSLATTLVSFTRVMLVRKKKRARWISPDRGIMRG

Specific function: Probable N-acetylglucosaminyltransferase that catalyzes the polymerization of single monomer units of UDP-N- acetylglucosamine to produce the linear homopolymer poly-beta-1,6- N-acetyl-D-glucosamine (PGA), a biofilm adhesin polysaccharide [H]

COG id: COG1215

COG function: function code M; Glycosyltransferases, probably involved in cell wall biogenesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787259, Length=441, Percent_Identity=88.2086167800454, Blast_Score=819, Evalue=0.0,
Organism=Escherichia coli, GI1786560, Length=211, Percent_Identity=28.436018957346, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001173 [H]

Pfam domain/function: PF00535 Glycos_transf_2 [H]

EC number: NA

Molecular weight: Translated: 50746; Mature: 50615

Theoretical pI: Translated: 9.36; Mature: 9.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNRIVSFFILCLVLFIPLSVAFFHSGELMMRFVFFWPFFMSIMWIVGGVYFWFHRERHW
CCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
QWGENAPAPQLKDNPPISIIIPCFNEEKNVEETIHAALAQRYENIEVIAVNDGSSDQTQA
CCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHH
ILDRLASLHPKLRVIHLAQNQGKAIALKTGAAAARSEYLVCIDGDALLDRDAAAYIVEPM
HHHHHHHCCCCEEEEEEECCCCCEEEEECCCCCCCCCEEEEECCCHHHCCCCHHHHCCCC
LYNPRVGAVTGNPRIRTRSTLVGKVQVGEFSSIIGLIKRTQRVYGNVFTVSGVIAAFRRS
EECCCEEEEECCCCEEEHHHEEEEEECCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
ALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRALCWILMPETLKGLWKQRLRWAQGG
HHHHCCCCCCCCEECCCCEEEEEEEEEEEEEECCCEEEEEECHHHHHHHHHHHHHHHCCH
AEVFLKNLNKLWQWKNHRMWPLFFEYCLTTTWAFTCLIGLFIFIAQQLGVPVNVELSHLA
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHH
ATHTAGILLCTLCLLQFLVSLIIENRYEQNMASSLFWIIWFPVVFWTLSLATTLVSFTRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MLVRKKKRARWISPDRGIMRG
HHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
TNRIVSFFILCLVLFIPLSVAFFHSGELMMRFVFFWPFFMSIMWIVGGVYFWFHRERHW
CHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
QWGENAPAPQLKDNPPISIIIPCFNEEKNVEETIHAALAQRYENIEVIAVNDGSSDQTQA
CCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHH
ILDRLASLHPKLRVIHLAQNQGKAIALKTGAAAARSEYLVCIDGDALLDRDAAAYIVEPM
HHHHHHHCCCCEEEEEEECCCCCEEEEECCCCCCCCCEEEEECCCHHHCCCCHHHHCCCC
LYNPRVGAVTGNPRIRTRSTLVGKVQVGEFSSIIGLIKRTQRVYGNVFTVSGVIAAFRRS
EECCCEEEEECCCCEEEHHHEEEEEECCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
ALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRALCWILMPETLKGLWKQRLRWAQGG
HHHHCCCCCCCCEECCCCEEEEEEEEEEEEEECCCEEEEEECHHHHHHHHHHHHHHHCCH
AEVFLKNLNKLWQWKNHRMWPLFFEYCLTTTWAFTCLIGLFIFIAQQLGVPVNVELSHLA
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHH
ATHTAGILLCTLCLLQFLVSLIIENRYEQNMASSLFWIIWFPVVFWTLSLATTLVSFTRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MLVRKKKRARWISPDRGIMRG
HHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]