| Definition | Escherichia fergusonii ATCC 35469 chromosome, complete genome. |
|---|---|
| Accession | NC_011740 |
| Length | 4,588,711 |
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The map label for this gene is pgaB [H]
Identifier: 218549245
GI number: 218549245
Start: 1954258
End: 1956276
Strand: Direct
Name: pgaB [H]
Synonym: EFER_1905
Alternate gene names: 218549245
Gene position: 1954258-1956276 (Clockwise)
Preceding gene: 218549244
Following gene: 218549246
Centisome position: 42.59
GC content: 45.72
Gene sequence:
>2019_bases ATGTTACGCAAAGGAAAAAGATCTCTCTTTATGCTGGTAAGCATTTTTATGCTTTCGGCATGTCTGAGCCAGCAAAAAAA TGAATTCACGCCACCACAACAACGTGAATCATTACAGGCTGAGCAACCATGGCCGCGTAATGGTTTTGTGGCGATTTCAT GGCATAACGTCGAAGACGAAGCTGCCGATCAGCGTTTTATGTCGGTGCGTACTTCTGCCCTGCGTGAACAATTCGCCTGG TTACGTGAGAACGGTTATCAACCTGTCAGCATTGCTCAAATTCGTGAGGCACATCGTGGTGGCAAACCGCTACCTGAAAA AGCGGTATTGCTGACTTTCGATGACGGTTACCAAAGTTTTTACACCCGTGTTTATCCACTCCTCAAAGCGTTCCAGTGGC CCGCATTATGGGCCCCGGTCGGCAGTTGGATCGATACACCACCTAATCAGCAAGTGAAATTTGGCGATGAAATGGTTGCC CGACACTATTTTGCCACCTGGCAACAAGTGCGTGAAATAGCACGTTCGAAACTGGTTGAAATAGCCTCCCACACCTGGAA TTCTCACTACGGCATAGAGGCTAATCCCACCGGTAGCCAATTACCCGTTTTTGTTAATCGCATGTATTTATTAAATAATG GGCGGTATGAAACCGAAGCGGAATATCGCCACCGTATTCGTCAGGATGCAATAAAAATGACCGCGCAGTTGCAGAAGAAT GCTGGCGTAACTGCCCAGGTCTTTGTCTGGCCCTATGGTGAAGCAAACGGTATTGCGATTGAGGAATTAAAAAAACTGGG CTATGACATGTTCTTTACGCTAGAGTCCGGGCTGGGAAACACTTCCCGGTTAGATTCCATTCCGCGTGTATTAATCGCCA ATAATCCATCACTTAAAGAGTTTGCTCAGCAAATTATTCGTGTGCAGGAAACATCACCAGAGCGAGTTATGCACATCGAT CTCGATTATGTCTACGATGCTGACCGCCAACAACAGGAGCGGAATCTCGATGTGTTGATTCAGCGGGTGAAAGATATGCA AATTTCCAGTGTGTACTTGCAGGCTTTTGCCGATCCGACAGGTAATGGTCTGGTTAAAGAGGTCTGGTTTCCTAATCGCC TGTTACCCATGAAAGCGGATTTATTTAACCGTGTTGCATGGCAATTAAGGACTCGTGCCGGAGTGGCCGTTTACGCCTGG ATGCCGGTGCTGAGTTGGGATTTAGATCCTACATTACCCCGGGTGGAATATTTGCCCACTGATAAGAAAACATCACAAAT TCATCCGTCGCAATACCGTCGTCTTTCTCCTTTTGATGACCGGGTACGTGAACAAATTAGCACCCTTTATGAAGATCTTG CAGGTCATGCGTCTTTTGACGGCATTTTGTTTCATGACGATGCCGTGTTGTCAGATTATGAAGATGCCAGTGCCCCTGCA ATAGCCGCTTATAAGAAAGCAGGTTTTTCGACAAGTATTCGTGAAATCCGCGAGGATCCTGAGCAATTTAAACGCTGGAC GCAATTTAAACGCCAGGCCTTAACAGACTTCACATTAGCGCTAAGTGCCAAAGTCAAAGCGGTTCGCGGTCCACAAATTA AGACTGCGCGAAATATTTTTGCCCTCCCCGTTATGCTCTCAGAAAGTGAAGCATGGTTTGCCCAAAACTATGCCGATTTT CTACAAAGTTATGACTGGACGGCAATCATGGCAATGCCCTATATGGAAGGCATTAATGAGAAATCTGCTGATAAGTGGCT GGTTCAACTGGTGAATCACATTAAAGCGATTCCACAGGCAAAAGATAAATCAGTTTTAGAATTACAGACACAAGACTGGA AAGCGAATGGTCAACATCAGGCTATTCAATCGCAACAAATCGCTCACTGGATGAGCCTTCTACAACTCAATGGAGTGAAA AACTATGGTTATTACCCTGATGATTTTCTGCGCAATCAGCCTGAAATAAAGGTCATTCGTCCTCAGTTCTCCACAGCCTG GTATCCAAAAAATGACTAA
Upstream 100 bases:
>100_bases CCGGAGCAACCTTACGCTATGAGAAACGACCTTACGACGGCGACAGAGAACATAACTTATATGTTGAATTTGATATGACA TTCAAATTTTAAGGATCATT
Downstream 100 bases:
>100_bases TCGCATCGTATCGTTTTTTATATTGTGTCTGGTGTTATTTATTCCTCTGAGTGTTGCGTTCTTCCACTCTGGCGAATTAA TGATGAGATTCGTGTTCTTC
Product: outer membrane N-deacetylase
Products: NA
Alternate protein names: PGA N-deacetylase; Poly-beta-1,6-GlcNAc N-deacetylase [H]
Number of amino acids: Translated: 672; Mature: 672
Protein sequence:
>672_residues MLRKGKRSLFMLVSIFMLSACLSQQKNEFTPPQQRESLQAEQPWPRNGFVAISWHNVEDEAADQRFMSVRTSALREQFAW LRENGYQPVSIAQIREAHRGGKPLPEKAVLLTFDDGYQSFYTRVYPLLKAFQWPALWAPVGSWIDTPPNQQVKFGDEMVA RHYFATWQQVREIARSKLVEIASHTWNSHYGIEANPTGSQLPVFVNRMYLLNNGRYETEAEYRHRIRQDAIKMTAQLQKN AGVTAQVFVWPYGEANGIAIEELKKLGYDMFFTLESGLGNTSRLDSIPRVLIANNPSLKEFAQQIIRVQETSPERVMHID LDYVYDADRQQQERNLDVLIQRVKDMQISSVYLQAFADPTGNGLVKEVWFPNRLLPMKADLFNRVAWQLRTRAGVAVYAW MPVLSWDLDPTLPRVEYLPTDKKTSQIHPSQYRRLSPFDDRVREQISTLYEDLAGHASFDGILFHDDAVLSDYEDASAPA IAAYKKAGFSTSIREIREDPEQFKRWTQFKRQALTDFTLALSAKVKAVRGPQIKTARNIFALPVMLSESEAWFAQNYADF LQSYDWTAIMAMPYMEGINEKSADKWLVQLVNHIKAIPQAKDKSVLELQTQDWKANGQHQAIQSQQIAHWMSLLQLNGVK NYGYYPDDFLRNQPEIKVIRPQFSTAWYPKND
Sequences:
>Translated_672_residues MLRKGKRSLFMLVSIFMLSACLSQQKNEFTPPQQRESLQAEQPWPRNGFVAISWHNVEDEAADQRFMSVRTSALREQFAW LRENGYQPVSIAQIREAHRGGKPLPEKAVLLTFDDGYQSFYTRVYPLLKAFQWPALWAPVGSWIDTPPNQQVKFGDEMVA RHYFATWQQVREIARSKLVEIASHTWNSHYGIEANPTGSQLPVFVNRMYLLNNGRYETEAEYRHRIRQDAIKMTAQLQKN AGVTAQVFVWPYGEANGIAIEELKKLGYDMFFTLESGLGNTSRLDSIPRVLIANNPSLKEFAQQIIRVQETSPERVMHID LDYVYDADRQQQERNLDVLIQRVKDMQISSVYLQAFADPTGNGLVKEVWFPNRLLPMKADLFNRVAWQLRTRAGVAVYAW MPVLSWDLDPTLPRVEYLPTDKKTSQIHPSQYRRLSPFDDRVREQISTLYEDLAGHASFDGILFHDDAVLSDYEDASAPA IAAYKKAGFSTSIREIREDPEQFKRWTQFKRQALTDFTLALSAKVKAVRGPQIKTARNIFALPVMLSESEAWFAQNYADF LQSYDWTAIMAMPYMEGINEKSADKWLVQLVNHIKAIPQAKDKSVLELQTQDWKANGQHQAIQSQQIAHWMSLLQLNGVK NYGYYPDDFLRNQPEIKVIRPQFSTAWYPKND >Mature_672_residues MLRKGKRSLFMLVSIFMLSACLSQQKNEFTPPQQRESLQAEQPWPRNGFVAISWHNVEDEAADQRFMSVRTSALREQFAW LRENGYQPVSIAQIREAHRGGKPLPEKAVLLTFDDGYQSFYTRVYPLLKAFQWPALWAPVGSWIDTPPNQQVKFGDEMVA RHYFATWQQVREIARSKLVEIASHTWNSHYGIEANPTGSQLPVFVNRMYLLNNGRYETEAEYRHRIRQDAIKMTAQLQKN AGVTAQVFVWPYGEANGIAIEELKKLGYDMFFTLESGLGNTSRLDSIPRVLIANNPSLKEFAQQIIRVQETSPERVMHID LDYVYDADRQQQERNLDVLIQRVKDMQISSVYLQAFADPTGNGLVKEVWFPNRLLPMKADLFNRVAWQLRTRAGVAVYAW MPVLSWDLDPTLPRVEYLPTDKKTSQIHPSQYRRLSPFDDRVREQISTLYEDLAGHASFDGILFHDDAVLSDYEDASAPA IAAYKKAGFSTSIREIREDPEQFKRWTQFKRQALTDFTLALSAKVKAVRGPQIKTARNIFALPVMLSESEAWFAQNYADF LQSYDWTAIMAMPYMEGINEKSADKWLVQLVNHIKAIPQAKDKSVLELQTQDWKANGQHQAIQSQQIAHWMSLLQLNGVK NYGYYPDDFLRNQPEIKVIRPQFSTAWYPKND
Specific function: Catalyzes the N-deacetylation of poly-beta-1,6-N-acetyl- D-glucosamine (PGA), a biofilm adhesin polysaccharide. N- deacetylation promotes PGA export through the PgaA porin [H]
COG id: COG0726
COG function: function code G; Predicted xylanase/chitin deacetylase
Gene ontology:
Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]
Metaboloic importance: Unknown [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the polysaccharide deacetylase family [H]
Homologues:
Organism=Escherichia coli, GI1787260, Length=672, Percent_Identity=80.5059523809524, Blast_Score=1160, Evalue=0.0, Organism=Escherichia coli, GI1786322, Length=110, Percent_Identity=32.7272727272727, Blast_Score=66, Evalue=7e-12,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR011330 - InterPro: IPR002509 [H]
Pfam domain/function: PF01522 Polysacc_deac_1 [H]
EC number: NA
Molecular weight: Translated: 77626; Mature: 77626
Theoretical pI: Translated: 8.28; Mature: 8.28
Prosite motif: PS00013 PROKAR_LIPOPROTEIN
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.1 %Cys (Translated Protein) 2.5 %Met (Translated Protein) 2.7 %Cys+Met (Translated Protein) 0.1 %Cys (Mature Protein) 2.5 %Met (Mature Protein) 2.7 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MLRKGKRSLFMLVSIFMLSACLSQQKNEFTPPQQRESLQAEQPWPRNGFVAISWHNVEDE CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCCEEEEEECCCCHH AADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGKPLPEKAVLLTFDDGYQSF HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCEEEEEECCCHHHH YTRVYPLLKAFQWPALWAPVGSWIDTPPNQQVKFGDEMVARHYFATWQQVREIARSKLVE HHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH IASHTWNSHYGIEANPTGSQLPVFVNRMYLLNNGRYETEAEYRHRIRQDAIKMTAQLQKN HHHHCCCCCCCCCCCCCCCCCHHHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHC AGVTAQVFVWPYGEANGIAIEELKKLGYDMFFTLESGLGNTSRLDSIPRVLIANNPSLKE CCCEEEEEEEECCCCCCCCHHHHHHCCCEEEEEEECCCCCCHHHHCCCEEEEECCCCHHH FAQQIIRVQETSPERVMHIDLDYVYDADRQQQERNLDVLIQRVKDMQISSVYLQAFADPT HHHHHHHHHCCCCCEEEEEEEHHHCCCHHHHHHCCHHHHHHHHHCCHHHHHHHHHHCCCC GNGLVKEVWFPNRLLPMKADLFNRVAWQLRTRAGVAVYAWMPVLSWDLDPTLPRVEYLPT CCCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEHHHHCCCCCCCCCCEEECCC DKKTSQIHPSQYRRLSPFDDRVREQISTLYEDLAGHASFDGILFHDDAVLSDYEDASAPA CCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHCCCCCCCCCH IAAYKKAGFSTSIREIREDPEQFKRWTQFKRQALTDFTLALSAKVKAVRGPQIKTARNIF HHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCEE ALPVMLSESEAWFAQNYADFLQSYDWTAIMAMPYMEGINEKSADKWLVQLVNHIKAIPQA EEEEEECCCHHHHHHHHHHHHHCCCCCEEEHHHHHHCCCCCHHHHHHHHHHHHHHHCCCC KDKSVLELQTQDWKANGQHQAIQSQQIAHWMSLLQLNGVKNYGYYPDDFLRNQPEIKVIR CCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCEEEEC PQFSTAWYPKND CCCCCCCCCCCC >Mature Secondary Structure MLRKGKRSLFMLVSIFMLSACLSQQKNEFTPPQQRESLQAEQPWPRNGFVAISWHNVEDE CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCCEEEEEECCCCHH AADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGKPLPEKAVLLTFDDGYQSF HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCEEEEEECCCHHHH YTRVYPLLKAFQWPALWAPVGSWIDTPPNQQVKFGDEMVARHYFATWQQVREIARSKLVE HHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH IASHTWNSHYGIEANPTGSQLPVFVNRMYLLNNGRYETEAEYRHRIRQDAIKMTAQLQKN HHHHCCCCCCCCCCCCCCCCCHHHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHC AGVTAQVFVWPYGEANGIAIEELKKLGYDMFFTLESGLGNTSRLDSIPRVLIANNPSLKE CCCEEEEEEEECCCCCCCCHHHHHHCCCEEEEEEECCCCCCHHHHCCCEEEEECCCCHHH FAQQIIRVQETSPERVMHIDLDYVYDADRQQQERNLDVLIQRVKDMQISSVYLQAFADPT HHHHHHHHHCCCCCEEEEEEEHHHCCCHHHHHHCCHHHHHHHHHCCHHHHHHHHHHCCCC GNGLVKEVWFPNRLLPMKADLFNRVAWQLRTRAGVAVYAWMPVLSWDLDPTLPRVEYLPT CCCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEHHHHCCCCCCCCCCEEECCC DKKTSQIHPSQYRRLSPFDDRVREQISTLYEDLAGHASFDGILFHDDAVLSDYEDASAPA CCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHCCCCCCCCCH IAAYKKAGFSTSIREIREDPEQFKRWTQFKRQALTDFTLALSAKVKAVRGPQIKTARNIF HHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCEE ALPVMLSESEAWFAQNYADFLQSYDWTAIMAMPYMEGINEKSADKWLVQLVNHIKAIPQA EEEEEECCCHHHHHHHHHHHHHCCCCCEEEHHHHHHCCCCCHHHHHHHHHHHHHHHCCCC KDKSVLELQTQDWKANGQHQAIQSQQIAHWMSLLQLNGVKNYGYYPDDFLRNQPEIKVIR CCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCEEEEC PQFSTAWYPKND CCCCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 6.0
TargetDB status: NA
Availability: NA
References: 11206551; 11258796 [H]