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The map label for this gene is pgaA [H]

Identifier: 218549244

GI number: 218549244

Start: 1951826

End: 1954249

Strand: Direct

Name: pgaA [H]

Synonym: EFER_1904

Alternate gene names: 218549244

Gene position: 1951826-1954249 (Clockwise)

Preceding gene: 218549233

Following gene: 218549245

Centisome position: 42.54

GC content: 48.56

Gene sequence:

>2424_bases
ATGTATTCAAACACCAGTAAAAGGCGCACCCGTGCAATCTTTGCCTTAAATTTTTTTACCGCAACGCTGTTAACACTCAG
TCCCACGGCTATATCCGCTACTACCAGCCAGTATGATGCATTGATCATTGAAGCTCGTAAGGGAAATACCCAACCAGCAT
TAAGCTGGTTTGCAGATAATACCGATCTCAATAGCACTCAGATTGCGGACTGGTTGCAGATTGCCTCCTGGGCCGGTCAG
GACCAGAAGGTTATTGCCATTTATAACCGCTTTGGTCATCAACCATTACCTGCTCGTGGTTATGCCCCTGTTGCAATGGC
ATATCGCAATTTACAACAGTGGCAGAAGTCGCTTCAGCTCTGGCAACTGGCGGTGTCAAAAGCGCCACAAAATACCGATT
ATCGTCGTGGGCAAATTCTCACCCTGGCCGATGCAGGCCAGTTCGATATCGCGCTTAATAAGCTCGAACAGTTGCAACGT
GAAGCACCAGACAAAACTAACACACTGGTTGCAGCGTATATCTACAAGCTGGCAGGTCGCCACCAGGATGAACTACTGGC
AATTACTGAAACCTTATCGGCGCCATCATCGTCCAACAATCTTGCGCAGGAATACGCCCAGGCGTTACGTAATAATCAGC
TGTCCGCCGCAATTAATGACGCCAGTTTAACCCCGGATATTCTCGCGGATATTCATGCGGAACTGGTCAGGCTCTCATTT
ATGCCAACCAGAAGTGAAAGTGAACGCTACGCTATTGCCGATCGTGCACTGGAACAATACACCGCTTTAGAGATCCTCTG
GAAAGATAACCCGGATCGTACCCTGCAATATCAACGCATTCAGATAGATCATCTCGGGGCATTATTAACCCGCGATCGTT
ATAAAGACGTCATTGCATACTATCAGCAATTAAAACAAGCGGGTCAAACCGTACCGTCCTGGGCGCAATATTGGGTGGCA
TCGGCCTATCTCAGAGATCACCAACCACAAAAAGCTCAGGCCATGATGTCTGATCTTTTTTACCATCAGGAAGTTCTGGC
CAGCACTTTATCGGAAGAAGAACGTGCGGATCTTTTTTATAGCCATCTTGAGAGTGAAAATTACGCCAGTGCATTGGCTG
TTACCCGCCATACGCTTAACAACACACCCGCCTGGCGTCGGGTAATGGGCTCACCGGTAACTTTGCCAAATGACAGCTGG
TTGCAGGGATATTCGTTTCTCTCTTCCGTGGCGCAATATGGCAATGATCTTCCTCAGGCGGAAAAAATAACCCGCAACCT
GGCAACCAATGCGCCTGGAAATCAGGGCTTACGCATCGATTATGCCAGCGTATTACAGGCGCGTGGCTGGCCGCATGCGG
CAGAAACGGAATTAAAAAAAGCCGAGGTGCTCGAACCGCGTAATATTTATCTGGAGGTTGAGCAAGCCTGGAATGCCTTA
ACATTACAAGAATGGCAACAGGCTACGGTATTAACTGACGATGTGGTGGCCCGTAATCCGCAAGATCCTGGCGTGGTACG
TTTAAAACGCGCCGTTGAGGTACATAATCTTGCTGAACTGCGTATTGCGGGCGCCTCAGGTCTGGATGCTGAAGGCCCGG
ACAGTGGCAAACATGATGTTAATTTTACCAGCGTCATTTACTCACCTCCGGTGAATGATAATTGGCGCGGTTTTGCCGGT
TTCGGCTATGCCGATGGGCAATTTAGCGAAGGAAAAGGCATTGTCCGCGACTGGCTGGCGGGGGTTGAATGGCGTTCACG
TGATCTCTGGCTGGAAGCAGAATACGCCGGACGCTATTTCAATCATGAACATAAACCCGGCGCAAGATTATCAGGCTGGT
ACGATTTCAACGACAACTGGCGTGTCGGCTCAGAGCTGCAACGCCTCTCCCATCGTGTACCGTTGCGGGCAATGAAAAAT
GGCATTACCGGCAATAGCGCACAAGCATATGTACGCTGGTATCAACATGAGCGCCGCCAGTACAGCCTCTCCTGGGCATT
TACTGATTTTTCCGACAATAACCAGCGGCACGAAGCGGCAATAGAAGGATCAGAACGTATCTGGTCTTCGGCAAATTTAA
TTATCGATTTCCAGCCAACTGTCTATTACCAACAGAATACGGAAACAGATACCCCTTACTTTAACCCGCGAAAAACGATC
GATATTGTTCCGGCTTTTGAGGCCAGTCATTTGCTCTGGCGTAATTATGAAAATAGCTGGCAACAAATATTCAGTGCTGG
TGTGGGCGCGTCCTGGCAAAAAGATTATGGCACGGATGTGATTACGCAACTCGGCTATGGGCAACGTATTAGCTGGAATG
ACGTCATTGATGCCGGAGCAACCTTACGCTATGAGAAACGACCTTACGACGGCGACAGAGAACATAACTTATATGTTGAA
TTTGATATGACATTCAAATTTTAA

Upstream 100 bases:

>100_bases
CGCAATTAACCTTACGGATGTTGAGGTACAGAACTCTTTATTTTCAAGCGACTGGAATCACTCGCTTGAGGAGTTCTTGT
TACAAATGGGGTAATACAGG

Downstream 100 bases:

>100_bases
GGATCATTATGTTACGCAAAGGAAAAAGATCTCTCTTTATGCTGGTAAGCATTTTTATGCTTTCGGCATGTCTGAGCCAG
CAAAAAAATGAATTCACGCC

Product: outer membrane protein PgaA

Products: NA

Alternate protein names: PGA export protein; Poly-beta-1,6-GlcNAc export protein [H]

Number of amino acids: Translated: 807; Mature: 807

Protein sequence:

>807_residues
MYSNTSKRRTRAIFALNFFTATLLTLSPTAISATTSQYDALIIEARKGNTQPALSWFADNTDLNSTQIADWLQIASWAGQ
DQKVIAIYNRFGHQPLPARGYAPVAMAYRNLQQWQKSLQLWQLAVSKAPQNTDYRRGQILTLADAGQFDIALNKLEQLQR
EAPDKTNTLVAAYIYKLAGRHQDELLAITETLSAPSSSNNLAQEYAQALRNNQLSAAINDASLTPDILADIHAELVRLSF
MPTRSESERYAIADRALEQYTALEILWKDNPDRTLQYQRIQIDHLGALLTRDRYKDVIAYYQQLKQAGQTVPSWAQYWVA
SAYLRDHQPQKAQAMMSDLFYHQEVLASTLSEEERADLFYSHLESENYASALAVTRHTLNNTPAWRRVMGSPVTLPNDSW
LQGYSFLSSVAQYGNDLPQAEKITRNLATNAPGNQGLRIDYASVLQARGWPHAAETELKKAEVLEPRNIYLEVEQAWNAL
TLQEWQQATVLTDDVVARNPQDPGVVRLKRAVEVHNLAELRIAGASGLDAEGPDSGKHDVNFTSVIYSPPVNDNWRGFAG
FGYADGQFSEGKGIVRDWLAGVEWRSRDLWLEAEYAGRYFNHEHKPGARLSGWYDFNDNWRVGSELQRLSHRVPLRAMKN
GITGNSAQAYVRWYQHERRQYSLSWAFTDFSDNNQRHEAAIEGSERIWSSANLIIDFQPTVYYQQNTETDTPYFNPRKTI
DIVPAFEASHLLWRNYENSWQQIFSAGVGASWQKDYGTDVITQLGYGQRISWNDVIDAGATLRYEKRPYDGDREHNLYVE
FDMTFKF

Sequences:

>Translated_807_residues
MYSNTSKRRTRAIFALNFFTATLLTLSPTAISATTSQYDALIIEARKGNTQPALSWFADNTDLNSTQIADWLQIASWAGQ
DQKVIAIYNRFGHQPLPARGYAPVAMAYRNLQQWQKSLQLWQLAVSKAPQNTDYRRGQILTLADAGQFDIALNKLEQLQR
EAPDKTNTLVAAYIYKLAGRHQDELLAITETLSAPSSSNNLAQEYAQALRNNQLSAAINDASLTPDILADIHAELVRLSF
MPTRSESERYAIADRALEQYTALEILWKDNPDRTLQYQRIQIDHLGALLTRDRYKDVIAYYQQLKQAGQTVPSWAQYWVA
SAYLRDHQPQKAQAMMSDLFYHQEVLASTLSEEERADLFYSHLESENYASALAVTRHTLNNTPAWRRVMGSPVTLPNDSW
LQGYSFLSSVAQYGNDLPQAEKITRNLATNAPGNQGLRIDYASVLQARGWPHAAETELKKAEVLEPRNIYLEVEQAWNAL
TLQEWQQATVLTDDVVARNPQDPGVVRLKRAVEVHNLAELRIAGASGLDAEGPDSGKHDVNFTSVIYSPPVNDNWRGFAG
FGYADGQFSEGKGIVRDWLAGVEWRSRDLWLEAEYAGRYFNHEHKPGARLSGWYDFNDNWRVGSELQRLSHRVPLRAMKN
GITGNSAQAYVRWYQHERRQYSLSWAFTDFSDNNQRHEAAIEGSERIWSSANLIIDFQPTVYYQQNTETDTPYFNPRKTI
DIVPAFEASHLLWRNYENSWQQIFSAGVGASWQKDYGTDVITQLGYGQRISWNDVIDAGATLRYEKRPYDGDREHNLYVE
FDMTFKF
>Mature_807_residues
MYSNTSKRRTRAIFALNFFTATLLTLSPTAISATTSQYDALIIEARKGNTQPALSWFADNTDLNSTQIADWLQIASWAGQ
DQKVIAIYNRFGHQPLPARGYAPVAMAYRNLQQWQKSLQLWQLAVSKAPQNTDYRRGQILTLADAGQFDIALNKLEQLQR
EAPDKTNTLVAAYIYKLAGRHQDELLAITETLSAPSSSNNLAQEYAQALRNNQLSAAINDASLTPDILADIHAELVRLSF
MPTRSESERYAIADRALEQYTALEILWKDNPDRTLQYQRIQIDHLGALLTRDRYKDVIAYYQQLKQAGQTVPSWAQYWVA
SAYLRDHQPQKAQAMMSDLFYHQEVLASTLSEEERADLFYSHLESENYASALAVTRHTLNNTPAWRRVMGSPVTLPNDSW
LQGYSFLSSVAQYGNDLPQAEKITRNLATNAPGNQGLRIDYASVLQARGWPHAAETELKKAEVLEPRNIYLEVEQAWNAL
TLQEWQQATVLTDDVVARNPQDPGVVRLKRAVEVHNLAELRIAGASGLDAEGPDSGKHDVNFTSVIYSPPVNDNWRGFAG
FGYADGQFSEGKGIVRDWLAGVEWRSRDLWLEAEYAGRYFNHEHKPGARLSGWYDFNDNWRVGSELQRLSHRVPLRAMKN
GITGNSAQAYVRWYQHERRQYSLSWAFTDFSDNNQRHEAAIEGSERIWSSANLIIDFQPTVYYQQNTETDTPYFNPRKTI
DIVPAFEASHLLWRNYENSWQQIFSAGVGASWQKDYGTDVITQLGYGQRISWNDVIDAGATLRYEKRPYDGDREHNLYVE
FDMTFKF

Specific function: Exports the biofilm adhesin polysaccharide poly-beta- 1,6-N-acetyl-D-glucosamine (PGA) across the outer membrane. The PGA transported seems to be partially N-deacetylated since N- deacetylation of PGA by PgaB is needed for PGA export through the PgaA pori

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 TPR repeats [H]

Homologues:

Organism=Escherichia coli, GI1787261, Length=807, Percent_Identity=77.3234200743494, Blast_Score=1307, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013026
- InterPro:   IPR011990
- InterPro:   IPR019734 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 91981; Mature: 91981

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS50293 TPR_REGION

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYSNTSKRRTRAIFALNFFTATLLTLSPTAISATTSQYDALIIEARKGNTQPALSWFADN
CCCCCCCHHHHEEEEHHHHHHHHHHCCCCCEECCCCCCCEEEEEECCCCCCCHHHEECCC
TDLNSTQIADWLQIASWAGQDQKVIAIYNRFGHQPLPARGYAPVAMAYRNLQQWQKSLQL
CCCCHHHHHHHHHHHHHCCCCCEEEEEEHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
WQLAVSKAPQNTDYRRGQILTLADAGQFDIALNKLEQLQREAPDKTNTLVAAYIYKLAGR
HHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCC
HQDELLAITETLSAPSSSNNLAQEYAQALRNNQLSAAINDASLTPDILADIHAELVRLSF
CCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHC
MPTRSESERYAIADRALEQYTALEILWKDNPDRTLQYQRIQIDHLGALLTRDRYKDVIAY
CCCCCCCCHHHHHHHHHHHHHEEEEEECCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHH
YQQLKQAGQTVPSWAQYWVASAYLRDHQPQKAQAMMSDLFYHQEVLASTLSEEERADLFY
HHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
SHLESENYASALAVTRHTLNNTPAWRRVMGSPVTLPNDSWLQGYSFLSSVAQYGNDLPQA
HHHCCCCHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHH
EKITRNLATNAPGNQGLRIDYASVLQARGWPHAAETELKKAEVLEPRNIYLEVEQAWNAL
HHHHHHHHCCCCCCCCCEEEHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEEHHHCCHH
TLQEWQQATVLTDDVVARNPQDPGVVRLKRAVEVHNLAELRIAGASGLDAEGPDSGKHDV
HHHHHHHHHHEEHHHHHCCCCCCCHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCC
NFTSVIYSPPVNDNWRGFAGFGYADGQFSEGKGIVRDWLAGVEWRSRDLWLEAEYAGRYF
EEEEEEECCCCCCCCCCEEECCCCCCCCCCCCCHHHHHHCCCCCCCCCEEEEEHHCCCCC
NHEHKPGARLSGWYDFNDNWRVGSELQRLSHRVPLRAMKNGITGNSAQAYVRWYQHERRQ
CCCCCCCCCCCCEEECCCCCCCHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHHHHE
YSLSWAFTDFSDNNQRHEAAIEGSERIWSSANLIIDFQPTVYYQQNTETDTPYFNPRKTI
EEEEEEEECCCCCCCHHHHHHCCHHHHHCCCCEEEEECCEEEEECCCCCCCCCCCCCCEE
DIVPAFEASHLLWRNYENSWQQIFSAGVGASWQKDYGTDVITQLGYGQRISWNDVIDAGA
EEEECCCHHHHHHHCHHHHHHHHHHHCCCCCCCHHCCHHHHHHCCCCCCCCHHHHHCCCC
TLRYEKRPYDGDREHNLYVEFDMTFKF
EEEECCCCCCCCCCCEEEEEEEEEECC
>Mature Secondary Structure
MYSNTSKRRTRAIFALNFFTATLLTLSPTAISATTSQYDALIIEARKGNTQPALSWFADN
CCCCCCCHHHHEEEEHHHHHHHHHHCCCCCEECCCCCCCEEEEEECCCCCCCHHHEECCC
TDLNSTQIADWLQIASWAGQDQKVIAIYNRFGHQPLPARGYAPVAMAYRNLQQWQKSLQL
CCCCHHHHHHHHHHHHHCCCCCEEEEEEHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
WQLAVSKAPQNTDYRRGQILTLADAGQFDIALNKLEQLQREAPDKTNTLVAAYIYKLAGR
HHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCC
HQDELLAITETLSAPSSSNNLAQEYAQALRNNQLSAAINDASLTPDILADIHAELVRLSF
CCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHC
MPTRSESERYAIADRALEQYTALEILWKDNPDRTLQYQRIQIDHLGALLTRDRYKDVIAY
CCCCCCCCHHHHHHHHHHHHHEEEEEECCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHH
YQQLKQAGQTVPSWAQYWVASAYLRDHQPQKAQAMMSDLFYHQEVLASTLSEEERADLFY
HHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
SHLESENYASALAVTRHTLNNTPAWRRVMGSPVTLPNDSWLQGYSFLSSVAQYGNDLPQA
HHHCCCCHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHH
EKITRNLATNAPGNQGLRIDYASVLQARGWPHAAETELKKAEVLEPRNIYLEVEQAWNAL
HHHHHHHHCCCCCCCCCEEEHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEEHHHCCHH
TLQEWQQATVLTDDVVARNPQDPGVVRLKRAVEVHNLAELRIAGASGLDAEGPDSGKHDV
HHHHHHHHHHEEHHHHHCCCCCCCHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCC
NFTSVIYSPPVNDNWRGFAGFGYADGQFSEGKGIVRDWLAGVEWRSRDLWLEAEYAGRYF
EEEEEEECCCCCCCCCCEEECCCCCCCCCCCCCHHHHHHCCCCCCCCCEEEEEHHCCCCC
NHEHKPGARLSGWYDFNDNWRVGSELQRLSHRVPLRAMKNGITGNSAQAYVRWYQHERRQ
CCCCCCCCCCCCEEECCCCCCCHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHHHHE
YSLSWAFTDFSDNNQRHEAAIEGSERIWSSANLIIDFQPTVYYQQNTETDTPYFNPRKTI
EEEEEEEECCCCCCCHHHHHHCCHHHHHCCCCEEEEECCEEEEECCCCCCCCCCCCCCEE
DIVPAFEASHLLWRNYENSWQQIFSAGVGASWQKDYGTDVITQLGYGQRISWNDVIDAGA
EEEECCCHHHHHHHCHHHHHHHHHHHCCCCCCCHHCCHHHHHHCCCCCCCCHHHHHCCCC
TLRYEKRPYDGDREHNLYVEFDMTFKF
EEEECCCCCCCCCCCEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]