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The map label for this gene is pcaJ [H]

Identifier: 218548987

GI number: 218548987

Start: 1681935

End: 1682591

Strand: Reverse

Name: pcaJ [H]

Synonym: EFER_1635

Alternate gene names: 218548987

Gene position: 1682591-1681935 (Counterclockwise)

Preceding gene: 218548988

Following gene: 218548986

Centisome position: 36.67

GC content: 50.53

Gene sequence:

>657_bases
ATGAAAAAGCTTTCGCGTGATGAAATGGCGAAACGCGTTGCCCAGGATATCCCGGAAGGCGCATACGTCAATCTTGGGAT
TGGTCTGCCGACCCGTATCGCCAATTATCTGCCTGCCGATAAAGAAATTTTTCTGCATAGCGAAAATGGTTTGTTGGGAA
TGGGGCCGAAGCCGAAAGAAGGCAATGAAGATCCTGAACTGATTAACGCAGGTAAAGAGTACGTGACATTGTTACAGGGT
GGTTGTTATTTCCACCATGGGGATTCCTTCGCCATGATGCGCGGTGGGCATCTGGATATTTGCGTATTGGGGGCTTATCA
GGTTTCCGCAACAGGTGATTTAGCTAACTGGAGCACCGGTGCACCGGACGCCATTCCCGCTGTCGGCGGTGCGATGGATT
TAGCCATTGGTGCTCGTCAGGTTTTTGTGATGATGGATCATTTGACCCGTGACGGGCAGAGCAAGTTGGTTGAGCGTTGC
AGTTATCCACTGACGGGCATCGGCTGCGTTAGCCGTATTTATACTGACCTGGCAGTCATTGATATCACCAATAACGGTCC
CGTGGTGCGAGAAATCTTTAACGGCATGTCGTTTGAAGAACTGCAACGTCTGACGCCTGTCACGCTCAGCTTTGAACAAC
TGGCGGAAAGCGCATAA

Upstream 100 bases:

>100_bases
CTGGATCCAGAACATATCATTACGCCAGGCATCTTCGTCCAACGCGTTTTTTCCCTTGAAAATCTGCAAGATAACGCACT
GAGCGCCTAAGGAGTCAATA

Downstream 100 bases:

>100_bases
GGAACTAATATGAATCAGGCATTTATCTGCGATGCAGTGCGAACTCCTTTTGGTCGATTTGGTGGCACGTTGGCAACGGT
TCGGGCTGATGATCTGGCTG

Product: 3-oxoadipate CoA-transferase subunit B

Products: NA

Alternate protein names: Beta-ketoadipate:succinyl-CoA transferase subunit B [H]

Number of amino acids: Translated: 218; Mature: 218

Protein sequence:

>218_residues
MKKLSRDEMAKRVAQDIPEGAYVNLGIGLPTRIANYLPADKEIFLHSENGLLGMGPKPKEGNEDPELINAGKEYVTLLQG
GCYFHHGDSFAMMRGGHLDICVLGAYQVSATGDLANWSTGAPDAIPAVGGAMDLAIGARQVFVMMDHLTRDGQSKLVERC
SYPLTGIGCVSRIYTDLAVIDITNNGPVVREIFNGMSFEELQRLTPVTLSFEQLAESA

Sequences:

>Translated_218_residues
MKKLSRDEMAKRVAQDIPEGAYVNLGIGLPTRIANYLPADKEIFLHSENGLLGMGPKPKEGNEDPELINAGKEYVTLLQG
GCYFHHGDSFAMMRGGHLDICVLGAYQVSATGDLANWSTGAPDAIPAVGGAMDLAIGARQVFVMMDHLTRDGQSKLVERC
SYPLTGIGCVSRIYTDLAVIDITNNGPVVREIFNGMSFEELQRLTPVTLSFEQLAESA
>Mature_218_residues
MKKLSRDEMAKRVAQDIPEGAYVNLGIGLPTRIANYLPADKEIFLHSENGLLGMGPKPKEGNEDPELINAGKEYVTLLQG
GCYFHHGDSFAMMRGGHLDICVLGAYQVSATGDLANWSTGAPDAIPAVGGAMDLAIGARQVFVMMDHLTRDGQSKLVERC
SYPLTGIGCVSRIYTDLAVIDITNNGPVVREIFNGMSFEELQRLTPVTLSFEQLAESA

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG2057

COG function: function code I; Acyl CoA:acetate/3-ketoacid CoA transferase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-oxoacid CoA-transferase subunit B family [H]

Homologues:

Organism=Homo sapiens, GI11545841, Length=203, Percent_Identity=44.3349753694581, Blast_Score=183, Evalue=1e-46,
Organism=Homo sapiens, GI4557817, Length=219, Percent_Identity=41.0958904109589, Blast_Score=177, Evalue=6e-45,
Organism=Escherichia coli, GI1788552, Length=201, Percent_Identity=42.2885572139304, Blast_Score=165, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI17531517, Length=212, Percent_Identity=46.2264150943396, Blast_Score=190, Evalue=5e-49,
Organism=Drosophila melanogaster, GI21358325, Length=198, Percent_Identity=45.959595959596, Blast_Score=179, Evalue=2e-45,
Organism=Drosophila melanogaster, GI24655794, Length=198, Percent_Identity=45.959595959596, Blast_Score=179, Evalue=2e-45,

Paralogues:

None

Copy number: 369 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012791
- InterPro:   IPR004165
- InterPro:   IPR004164 [H]

Pfam domain/function: PF01144 CoA_trans [H]

EC number: =2.8.3.6 [H]

Molecular weight: Translated: 23576; Mature: 23576

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS01274 COA_TRANSF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLSRDEMAKRVAQDIPEGAYVNLGIGLPTRIANYLPADKEIFLHSENGLLGMGPKPKE
CCCCCHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHCCCCCEEEEECCCCEECCCCCCCC
GNEDPELINAGKEYVTLLQGGCYFHHGDSFAMMRGGHLDICVLGAYQVSATGDLANWSTG
CCCCHHHHHCCHHHHHHHHCCEEEEECCCEEEEECCCEEEEEEEEEEEECCCCCCCCCCC
APDAIPAVGGAMDLAIGARQVFVMMDHLTRDGQSKLVERCSYPLTGIGCVSRIYTDLAVI
CCCCHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHCEEEE
DITNNGPVVREIFNGMSFEELQRLTPVTLSFEQLAESA
EECCCCCHHHHHHCCCCHHHHHHCCCCEECHHHHHCCC
>Mature Secondary Structure
MKKLSRDEMAKRVAQDIPEGAYVNLGIGLPTRIANYLPADKEIFLHSENGLLGMGPKPKE
CCCCCHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHCCCCCEEEEECCCCEECCCCCCCC
GNEDPELINAGKEYVTLLQGGCYFHHGDSFAMMRGGHLDICVLGAYQVSATGDLANWSTG
CCCCHHHHHCCHHHHHHHHCCEEEEECCCEEEEECCCEEEEEEEEEEEECCCCCCCCCCC
APDAIPAVGGAMDLAIGARQVFVMMDHLTRDGQSKLVERCSYPLTGIGCVSRIYTDLAVI
CCCCHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHCEEEE
DITNNGPVVREIFNGMSFEELQRLTPVTLSFEQLAESA
EECCCCCHHHHHHCCCCHHHHHHCCCCEECHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8063101; 8125318 [H]