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The map label for this gene is paaJ [H]

Identifier: 218548986

GI number: 218548986

Start: 1680723

End: 1681925

Strand: Reverse

Name: paaJ [H]

Synonym: EFER_1634

Alternate gene names: 218548986

Gene position: 1681925-1680723 (Counterclockwise)

Preceding gene: 218548987

Following gene: 218548985

Centisome position: 36.65

GC content: 54.61

Gene sequence:

>1203_bases
ATGAATCAGGCATTTATCTGCGATGCAGTGCGAACTCCTTTTGGTCGATTTGGTGGCACGTTGGCAACGGTTCGGGCTGA
TGATCTGGCTGCAATTCCGCTTAAAGCGCTGTTATCACGCAATCCAGGACTCGATCCGGCATTTATTGACGATGTGATTT
ACGGTTGTGCCAACCAGGCAGGGGAAGATAACCGTAATGTGGCACGAATGGCATTACTGTTAGCCGGGCTGCCGGAAAGC
GTGCCGGGAAGCACTATAAATCGACTTTGTGGTTCAAGTCTTGATGCCATTGGTGTTGCTGCCCGGGCGATTAAAAGCGG
TGAAACACATTTGATGATCGCCGGTGGCGTGGAAAGCATGTCGCGAGCGCCGTTTGTCATGGGGAAAGCGGAATCCGCAT
GGAATCGCAACATGAAGATGGAAGATACCACCATTGGATGGCGCTTTATTAACGCTCAGATGAAAGCGCGGTATGGCGTT
GATTCAATGCCAGAAACAGCCGAAAACGTCGCCGGGCAATTTAGCATCAGTCGTGCGGATCAAGACGCTTTTGCCCTGCG
TAGTCAGCAACGTACTGCTCAGGCACAGCAAGATGGTCTGTTTGAAGAAGAATTGACGGAAGTTAGCGTGCCGCAGCGTA
AAGGCCCGGCGCAACTCTTTATCCGCGATGAGCATCCCCGTGCAACCTCACTGGAGGCGCTGGCAGGTTTGAAGGCGGTG
GTACGTGAAGGTGGCTCCGTCACTGCGGGGAATGCCTCCGGTGTCAACGATGGCGCATGTGCATTATTGCTCGCCAGTGA
ACAAGCAATGCGGCAGCATGATTTGCAACCGTTAGTTCGGGTGGTGGGTATTGCAACCGCAGGCGTTGCACCGCGTATTA
TGGGGTTTGGACCCGCGCCTGCGGTGCGCAAAGTTCTGGCGCAGACAGGGTTAACGCTGGATCAAATGGATGTTATAGAA
CTCAATGAAGCCTTTGCCGCTCAGGCGCTGGCGGTAATGCGTGATTTAGGGTTACCGGATGACGCGACACATGTGAATCC
GAATGGCGGGGCGATTGCGCTGGGGCATCCGTTGGGTGCATCTGGTGGGCGGCTGGCGATGACAGCGGCCCGACAACTGC
AACGTACCGGGGGGCGTTATGCGCTATGCACTATGTGTATCGGTGTCGGGCAGGGGATCGCCCTGATTATTGAACGAGTG
TAA

Upstream 100 bases:

>100_bases
GCGAGAAATCTTTAACGGCATGTCGTTTGAAGAACTGCAACGTCTGACGCCTGTCACGCTCAGCTTTGAACAACTGGCGG
AAAGCGCATAAGGAACTAAT

Downstream 100 bases:

>100_bases
GGAGAAGTGATGGCAGTGTTGACTCCGCTGATGCGTGGCAGTGCGCTAACGGACTGTTTTACCGATGAGTGCTTATTGCA
GGGGATGTTGGATTTCGAGG

Product: Beta-ketoadipyl CoA thiolase

Products: NA

Alternate protein names: 3-oxoadipyl-CoA thiolase [H]

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MNQAFICDAVRTPFGRFGGTLATVRADDLAAIPLKALLSRNPGLDPAFIDDVIYGCANQAGEDNRNVARMALLLAGLPES
VPGSTINRLCGSSLDAIGVAARAIKSGETHLMIAGGVESMSRAPFVMGKAESAWNRNMKMEDTTIGWRFINAQMKARYGV
DSMPETAENVAGQFSISRADQDAFALRSQQRTAQAQQDGLFEEELTEVSVPQRKGPAQLFIRDEHPRATSLEALAGLKAV
VREGGSVTAGNASGVNDGACALLLASEQAMRQHDLQPLVRVVGIATAGVAPRIMGFGPAPAVRKVLAQTGLTLDQMDVIE
LNEAFAAQALAVMRDLGLPDDATHVNPNGGAIALGHPLGASGGRLAMTAARQLQRTGGRYALCTMCIGVGQGIALIIERV

Sequences:

>Translated_400_residues
MNQAFICDAVRTPFGRFGGTLATVRADDLAAIPLKALLSRNPGLDPAFIDDVIYGCANQAGEDNRNVARMALLLAGLPES
VPGSTINRLCGSSLDAIGVAARAIKSGETHLMIAGGVESMSRAPFVMGKAESAWNRNMKMEDTTIGWRFINAQMKARYGV
DSMPETAENVAGQFSISRADQDAFALRSQQRTAQAQQDGLFEEELTEVSVPQRKGPAQLFIRDEHPRATSLEALAGLKAV
VREGGSVTAGNASGVNDGACALLLASEQAMRQHDLQPLVRVVGIATAGVAPRIMGFGPAPAVRKVLAQTGLTLDQMDVIE
LNEAFAAQALAVMRDLGLPDDATHVNPNGGAIALGHPLGASGGRLAMTAARQLQRTGGRYALCTMCIGVGQGIALIIERV
>Mature_400_residues
MNQAFICDAVRTPFGRFGGTLATVRADDLAAIPLKALLSRNPGLDPAFIDDVIYGCANQAGEDNRNVARMALLLAGLPES
VPGSTINRLCGSSLDAIGVAARAIKSGETHLMIAGGVESMSRAPFVMGKAESAWNRNMKMEDTTIGWRFINAQMKARYGV
DSMPETAENVAGQFSISRADQDAFALRSQQRTAQAQQDGLFEEELTEVSVPQRKGPAQLFIRDEHPRATSLEALAGLKAV
VREGGSVTAGNASGVNDGACALLLASEQAMRQHDLQPLVRVVGIATAGVAPRIMGFGPAPAVRKVLAQTGLTLDQMDVIE
LNEAFAAQALAVMRDLGLPDDATHVNPNGGAIALGHPLGASGGRLAMTAARQLQRTGGRYALCTMCIGVGQGIALIIERV

Specific function: Catalyzes thiolytic cleavage of beta-ketoadipyl-CoA to succinyl-CoA and acetyl-CoA [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=397, Percent_Identity=43.3249370277078, Blast_Score=296, Evalue=2e-80,
Organism=Homo sapiens, GI148539872, Length=398, Percent_Identity=39.1959798994975, Blast_Score=257, Evalue=2e-68,
Organism=Homo sapiens, GI4501853, Length=395, Percent_Identity=40.253164556962, Blast_Score=223, Evalue=3e-58,
Organism=Homo sapiens, GI4557237, Length=401, Percent_Identity=34.1645885286783, Blast_Score=218, Evalue=6e-57,
Organism=Homo sapiens, GI4504327, Length=423, Percent_Identity=32.6241134751773, Blast_Score=182, Evalue=4e-46,
Organism=Homo sapiens, GI194353979, Length=390, Percent_Identity=30, Blast_Score=131, Evalue=1e-30,
Organism=Escherichia coli, GI1787663, Length=401, Percent_Identity=68.3291770573566, Blast_Score=548, Evalue=1e-157,
Organism=Escherichia coli, GI1788554, Length=404, Percent_Identity=45.049504950495, Blast_Score=282, Evalue=3e-77,
Organism=Escherichia coli, GI48994986, Length=405, Percent_Identity=42.7160493827161, Blast_Score=281, Evalue=5e-77,
Organism=Escherichia coli, GI87082165, Length=401, Percent_Identity=42.8927680798005, Blast_Score=262, Evalue=2e-71,
Organism=Escherichia coli, GI1788683, Length=426, Percent_Identity=30.7511737089202, Blast_Score=150, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI133906874, Length=399, Percent_Identity=43.1077694235589, Blast_Score=311, Evalue=4e-85,
Organism=Caenorhabditis elegans, GI25147385, Length=405, Percent_Identity=33.0864197530864, Blast_Score=214, Evalue=6e-56,
Organism=Caenorhabditis elegans, GI17535921, Length=401, Percent_Identity=33.9152119700748, Blast_Score=198, Evalue=5e-51,
Organism=Caenorhabditis elegans, GI17551802, Length=436, Percent_Identity=30.7339449541284, Blast_Score=170, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17535917, Length=402, Percent_Identity=28.8557213930348, Blast_Score=145, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6325229, Length=400, Percent_Identity=34.75, Blast_Score=224, Evalue=2e-59,
Organism=Saccharomyces cerevisiae, GI6322031, Length=400, Percent_Identity=37.5, Blast_Score=214, Evalue=3e-56,
Organism=Drosophila melanogaster, GI24655093, Length=401, Percent_Identity=44.3890274314214, Blast_Score=317, Evalue=6e-87,
Organism=Drosophila melanogaster, GI17648125, Length=403, Percent_Identity=41.439205955335, Blast_Score=267, Evalue=1e-71,
Organism=Drosophila melanogaster, GI24640423, Length=402, Percent_Identity=31.3432835820896, Blast_Score=199, Evalue=3e-51,
Organism=Drosophila melanogaster, GI17137578, Length=424, Percent_Identity=32.7830188679245, Blast_Score=184, Evalue=9e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012793
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.174 [H]

Molecular weight: Translated: 42042; Mature: 42042

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQAFICDAVRTPFGRFGGTLATVRADDLAAIPLKALLSRNPGLDPAFIDDVIYGCANQA
CCCCEEEHHHHCCHHHHCCEEEEEECCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCC
GEDNRNVARMALLLAGLPESVPGSTINRLCGSSLDAIGVAARAIKSGETHLMIAGGVESM
CCCCHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEECCHHHH
SRAPFVMGKAESAWNRNMKMEDTTIGWRFINAQMKARYGVDSMPETAENVAGQFSISRAD
CCCCEEEECCHHHHCCCCCCCCCCCCEEEECHHHHHHCCCCCCCHHHHHHCCCEEECCCC
QDAFALRSQQRTAQAQQDGLFEEELTEVSVPQRKGPAQLFIRDEHPRATSLEALAGLKAV
HHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHH
VREGGSVTAGNASGVNDGACALLLASEQAMRQHDLQPLVRVVGIATAGVAPRIMGFGPAP
HHCCCCEECCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCH
AVRKVLAQTGLTLDQMDVIELNEAFAAQALAVMRDLGLPDDATHVNPNGGAIALGHPLGA
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCEEEECCCCCC
SGGRLAMTAARQLQRTGGRYALCTMCIGVGQGIALIIERV
CCCCHHHHHHHHHHHCCCCEEHHHHHHHCCCCHHHHEECC
>Mature Secondary Structure
MNQAFICDAVRTPFGRFGGTLATVRADDLAAIPLKALLSRNPGLDPAFIDDVIYGCANQA
CCCCEEEHHHHCCHHHHCCEEEEEECCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCC
GEDNRNVARMALLLAGLPESVPGSTINRLCGSSLDAIGVAARAIKSGETHLMIAGGVESM
CCCCHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEECCHHHH
SRAPFVMGKAESAWNRNMKMEDTTIGWRFINAQMKARYGVDSMPETAENVAGQFSISRAD
CCCCEEEECCHHHHCCCCCCCCCCCCEEEECHHHHHHCCCCCCCHHHHHHCCCEEECCCC
QDAFALRSQQRTAQAQQDGLFEEELTEVSVPQRKGPAQLFIRDEHPRATSLEALAGLKAV
HHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHH
VREGGSVTAGNASGVNDGACALLLASEQAMRQHDLQPLVRVVGIATAGVAPRIMGFGPAP
HHCCCCEECCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCH
AVRKVLAQTGLTLDQMDVIELNEAFAAQALAVMRDLGLPDDATHVNPNGGAIALGHPLGA
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCEEEECCCCCC
SGGRLAMTAARQLQRTGGRYALCTMCIGVGQGIALIIERV
CCCCHHHHHHHHHHHCCCCEEHHHHHHHCCCCHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8125318 [H]