Click here to switch to the map view.

The map label for this gene is pcaB [H]

Identifier: 218548985

GI number: 218548985

Start: 1679361

End: 1680716

Strand: Reverse

Name: pcaB [H]

Synonym: EFER_1633

Alternate gene names: 218548985

Gene position: 1680716-1679361 (Counterclockwise)

Preceding gene: 218548986

Following gene: 218548984

Centisome position: 36.63

GC content: 53.39

Gene sequence:

>1356_bases
GTGATGGCAGTGTTGACTCCGCTGATGCGTGGCAGTGCGCTAACGGACTGTTTTACCGATGAGTGCTTATTGCAGGGGAT
GTTGGATTTCGAGGCAGCCCTGGCACTGGCTCAGGCACGTTGTGGGGTGATCCCGCCATCTGCGGTCGCGGCAATTGCGG
CTGCATGCAGAGCTGAAAATATTGACGTTCAGGCGCTGGGGGGGGATGCGGCGCTGGCGGGGAATCTGGCTATCCCACTG
GTGAAGCAATTAACCGCGCAGGTGAAGAAAAATAATGCCGCAGCGGCACGTTACGTTCACTGGGGAGCAACCAGCCAGGA
TGTCATTGATACCGGGTTAATACTGCAATTACGCCAGGCACTTCAACTTACCCAACAGAGTGTAAACCAGTTGCTTGCTA
CGTTCGCGGAACAGGTTTTTCGTTATCAGAAGACGGTAATGCCAGGTCGTACATGGATGCAGCAAGCGCTCCCGGTGACC
CTGGGCTTAAAACTTGCCGGAACGCTGGATGCATTACTTCGCTGGCAGGTGCGTTTAGAGGAGTTACTGCCGCGTGTGTT
GGTGCTGCAATTTGGTGGTGCGGCAGGAACTCTGGCTTCGCTTAAGCAGGATGCACTACCGGTGGCTGCCGAACTGGCGA
AAGTCCTGGGATTGACGCTGCCGGATTTGCCATGGCATACGCAACGGGATCGCTTACTGGAAATTGCCGCCTGGTATGCG
GGCACAGGGGCGACACTGGCAAAATTTGCGCAGGATTTTGCACTGTTAATGCAGACGGAAGTTGCAGAAGTGAGTGAACC
TGCCGTTGCCGGACGAGGCGGTTCATCCGCAATGCCCCATAAGCGTAACCCTGTCATTTGTGCCTCTATTCTTACCGCTG
CACAGCGTACCCCGGGTTTAATGGCTACCCTGTATGCCAGCCAGTTACAACAGCATGAACGCGCACTTGGTGGCTGGCAA
GCTGAGTGGGAAACCTTACCAGAGCTGATTATGTTGACAGGGAATATTATCGCGCAAAGCCAGGAACTGGTGCAGGGAAT
GCAAGTGTACCCACAAAAGATGCGTGAAAATCTCGATATTACCCACGGGTTATTGATGGCGGAGTCAGTGACTTTGGCGC
TGGCGGAGTTTATTGGTAAACAGGATGCCCATCATCTGGTGGAAGTGCTTTGTCGGCAAGCAATCGAACAGCAACACGCC
TTGTACGACGTACTGGTAAACGATACGCGTATTACCTCATGGCTTAACGCCACCCAAATTAAAAATTTGCTGGAACCTAC
CGCAGCGACCGGCAGTGCACAGGTCTTTATCGACCGGGTGCTGGCTCGCTATAAGGAACAACAAAATGAAAATTAA

Upstream 100 bases:

>100_bases
CCGACAACTGCAACGTACCGGGGGGCGTTATGCGCTATGCACTATGTGTATCGGTGTCGGGCAGGGGATCGCCCTGATTA
TTGAACGAGTGTAAGGAGAA

Downstream 100 bases:

>100_bases
TTACCAGATAGAAGGGCCGGAGAATGCGCCGGTTATTGTGCTGTCGAATTCACTGGGCACCACGCTTTCGATGTGGCAAC
CACAAATATCCGCACTGACT

Product: 3-carboxy-cis,cis-muconate cycloisomerase

Products: NA

Alternate protein names: 3-carboxymuconate lactonizing enzyme; CMLE [H]

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MMAVLTPLMRGSALTDCFTDECLLQGMLDFEAALALAQARCGVIPPSAVAAIAAACRAENIDVQALGGDAALAGNLAIPL
VKQLTAQVKKNNAAAARYVHWGATSQDVIDTGLILQLRQALQLTQQSVNQLLATFAEQVFRYQKTVMPGRTWMQQALPVT
LGLKLAGTLDALLRWQVRLEELLPRVLVLQFGGAAGTLASLKQDALPVAAELAKVLGLTLPDLPWHTQRDRLLEIAAWYA
GTGATLAKFAQDFALLMQTEVAEVSEPAVAGRGGSSAMPHKRNPVICASILTAAQRTPGLMATLYASQLQQHERALGGWQ
AEWETLPELIMLTGNIIAQSQELVQGMQVYPQKMRENLDITHGLLMAESVTLALAEFIGKQDAHHLVEVLCRQAIEQQHA
LYDVLVNDTRITSWLNATQIKNLLEPTAATGSAQVFIDRVLARYKEQQNEN

Sequences:

>Translated_451_residues
MMAVLTPLMRGSALTDCFTDECLLQGMLDFEAALALAQARCGVIPPSAVAAIAAACRAENIDVQALGGDAALAGNLAIPL
VKQLTAQVKKNNAAAARYVHWGATSQDVIDTGLILQLRQALQLTQQSVNQLLATFAEQVFRYQKTVMPGRTWMQQALPVT
LGLKLAGTLDALLRWQVRLEELLPRVLVLQFGGAAGTLASLKQDALPVAAELAKVLGLTLPDLPWHTQRDRLLEIAAWYA
GTGATLAKFAQDFALLMQTEVAEVSEPAVAGRGGSSAMPHKRNPVICASILTAAQRTPGLMATLYASQLQQHERALGGWQ
AEWETLPELIMLTGNIIAQSQELVQGMQVYPQKMRENLDITHGLLMAESVTLALAEFIGKQDAHHLVEVLCRQAIEQQHA
LYDVLVNDTRITSWLNATQIKNLLEPTAATGSAQVFIDRVLARYKEQQNEN
>Mature_451_residues
MMAVLTPLMRGSALTDCFTDECLLQGMLDFEAALALAQARCGVIPPSAVAAIAAACRAENIDVQALGGDAALAGNLAIPL
VKQLTAQVKKNNAAAARYVHWGATSQDVIDTGLILQLRQALQLTQQSVNQLLATFAEQVFRYQKTVMPGRTWMQQALPVT
LGLKLAGTLDALLRWQVRLEELLPRVLVLQFGGAAGTLASLKQDALPVAAELAKVLGLTLPDLPWHTQRDRLLEIAAWYA
GTGATLAKFAQDFALLMQTEVAEVSEPAVAGRGGSSAMPHKRNPVICASILTAAQRTPGLMATLYASQLQQHERALGGWQ
AEWETLPELIMLTGNIIAQSQELVQGMQVYPQKMRENLDITHGLLMAESVTLALAEFIGKQDAHHLVEVLCRQAIEQQHA
LYDVLVNDTRITSWLNATQIKNLLEPTAATGSAQVFIDRVLARYKEQQNEN

Specific function: Catalyzes an anti cycloisomerization [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=367, Percent_Identity=25.6130790190736, Blast_Score=82, Evalue=1e-15,
Organism=Escherichia coli, GI1790398, Length=327, Percent_Identity=26.2996941896024, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323391, Length=356, Percent_Identity=24.438202247191, Blast_Score=77, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24647570, Length=442, Percent_Identity=25.7918552036199, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24640179, Length=348, Percent_Identity=24.4252873563218, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24640177, Length=348, Percent_Identity=24.4252873563218, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR012789 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =5.5.1.2 [H]

Molecular weight: Translated: 48933; Mature: 48933

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHCCCCCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure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HHHHCCCCCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]