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The map label for this gene is narQ [H]

Identifier: 218548098

GI number: 218548098

Start: 726770

End: 728482

Strand: Reverse

Name: narQ [H]

Synonym: EFER_0709

Alternate gene names: 218548098

Gene position: 728482-726770 (Counterclockwise)

Preceding gene: 218548102

Following gene: 218548097

Centisome position: 15.88

GC content: 49.21

Gene sequence:

>1713_bases
ATGCGAGTGATCGTTAAACGTCCCGTCTCGGCCAGTCTGGCCAGGGCCTTTTTTTATATTGTCCTGTTGTCCATTCTCTC
CACCGGTATCGCGCTATTGACACTGGCGAGCAGTTTGCGTGACGCAGAGGCCATCAATATTGCCGGTTCACTGCGAATGC
AAAGCTATCGGCTAGGCTACGATTTACAGCGGCAAAGCCCGCAACTGCATGATCATCGTCAGCTTTTCCAACAAGCGCTG
CATTCACCGGTATTAAACAATCTCAACGCATGGTATGTGCCACAAGCGGTGAAAACTCGCTATGCCCATCTTCACGCCAA
TTGGCTGGAGATGAATAAACGGCTGAACGAAGGCGATATTGACTGGTATCAGGAAAATATTAATAACTATGTCAACCAGA
TAGATCTCTTCGTTCTGGCTTTGCAACACTATGCTGAACGTAAAATGATGTTGGTTGTGGCGATATCGCTGGCCGGGGGA
ATAGGTATTTTTACCCTCGTCTTTTTTACCCTACGGCGGATTCGCCATCAGGTGGTGCAGCCATTGAATCATTTGGTGAT
GGCCAGCCAGCGAATGGAGCAAGGTCAGTTTGATTCGTTGCCCTTAGATACCAGTCTCCCCAATGAATTAGGTTTATTGG
CCAAGACCTTCACTCAAATGTCGAGTGAATTACAAAAGCTGTATCTGTCGCTGGAAGCTTCGGTTGCAGAAAAAACGCGT
GATTTGCATGAGGCCAAGCGCCGTCTGGAGGTACTGTATCAATGCTCTCAGGCGCTAAATACCAGCCAGATTGATGTTCA
CTGTTTCCGCCTCATATTGCAGATAGTCCGGGAACATGATGCAGCCTGCTGGCTGGAACTTACTGTTGGCGATAACTGGC
GTATTAGTGAAGGGCACAAAAATGGCGAATTACCCTTACAGATTATACCGGTGACAATGCAGGATACTGTTTATGGCGAG
TTGCAATGGCAAAGCGAGAATGCTTCTGCTTTCGCACCGTTAATGAATAGCGTATCGACCATGTTAGGTCGTGGTTTGTA
CTTTAATCAGGCACAGAAGCATTTCCAGCAACTGCTACTGATGGAAGAACGCGCCACCATCGCACGGGAATTACATGATT
CGCTGGCGCAGGTGCTCTCTTATCTGCGTATTCAGCTCACGCTTCTGAAACGATCAATTCCGCAAGATAACGACACAGCG
CAAAGTATCATGGCGGATTTTTCACGCGCCTTAAATGATGCGTACCAGCAGCTGCGTGAACTGCTCACAACGTTCCGCCT
GACATTGCAACAGGCTGATCTGCCTTCTGCACTACGCGAAATGCTGGATACCCTGCAAAACCAAACTTCCGCTAAGCTCA
GCCTCGACTGTCGCCTGCCAACACTGGCGCTGGATGCGCAAATGCAGGTGCATTTGTTACAGATTATTCGTGAAGCGGTT
GTTAATGCGATCAAACATGCTAACGCCAGTGAGATCGCCGTCAGTTGCGTAACTGCTCCTGACGGTAACCACACCGTGTT
TATTCGTGATAACGGAATTGGCATTGGCGACCCCACTGAACCTGAGGGGCACTACGGATTGAATATCATGCGCGAACGCG
CGGAACGGCTGGGTGGTACGCTAAGTTTTTCCCAACCGTCTGGTGGCGGTACTTTGGTGAGTATCAGTTTTCGATCCGTA
GAAGATGAACAGAACCATCCTTCAGGCGCGTGA

Upstream 100 bases:

>100_bases
ACATGTGGCAGATCAATTTATTACACAGCTGATATTATTTTGGATGTAATTATCTGAAACGCTTTGTTTAATTTCATATT
GACCCTGTTTCGCGGAGAAA

Downstream 100 bases:

>100_bases
AACAGGCACGATAATTTACATTATCTCCTTTTTTTCTCCACGATTGGCCCGTACCTTGCCGCTACAGTGAAACAAGGTAT
GCCCATAACGATACGCAGAA

Product: nitrate/nitrite sensor protein NarQ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 570; Mature: 570

Protein sequence:

>570_residues
MRVIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAINIAGSLRMQSYRLGYDLQRQSPQLHDHRQLFQQAL
HSPVLNNLNAWYVPQAVKTRYAHLHANWLEMNKRLNEGDIDWYQENINNYVNQIDLFVLALQHYAERKMMLVVAISLAGG
IGIFTLVFFTLRRIRHQVVQPLNHLVMASQRMEQGQFDSLPLDTSLPNELGLLAKTFTQMSSELQKLYLSLEASVAEKTR
DLHEAKRRLEVLYQCSQALNTSQIDVHCFRLILQIVREHDAACWLELTVGDNWRISEGHKNGELPLQIIPVTMQDTVYGE
LQWQSENASAFAPLMNSVSTMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIPQDNDTA
QSIMADFSRALNDAYQQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAKLSLDCRLPTLALDAQMQVHLLQIIREAV
VNAIKHANASEIAVSCVTAPDGNHTVFIRDNGIGIGDPTEPEGHYGLNIMRERAERLGGTLSFSQPSGGGTLVSISFRSV
EDEQNHPSGA

Sequences:

>Translated_570_residues
MRVIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAINIAGSLRMQSYRLGYDLQRQSPQLHDHRQLFQQAL
HSPVLNNLNAWYVPQAVKTRYAHLHANWLEMNKRLNEGDIDWYQENINNYVNQIDLFVLALQHYAERKMMLVVAISLAGG
IGIFTLVFFTLRRIRHQVVQPLNHLVMASQRMEQGQFDSLPLDTSLPNELGLLAKTFTQMSSELQKLYLSLEASVAEKTR
DLHEAKRRLEVLYQCSQALNTSQIDVHCFRLILQIVREHDAACWLELTVGDNWRISEGHKNGELPLQIIPVTMQDTVYGE
LQWQSENASAFAPLMNSVSTMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIPQDNDTA
QSIMADFSRALNDAYQQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAKLSLDCRLPTLALDAQMQVHLLQIIREAV
VNAIKHANASEIAVSCVTAPDGNHTVFIRDNGIGIGDPTEPEGHYGLNIMRERAERLGGTLSFSQPSGGGTLVSISFRSV
EDEQNHPSGA
>Mature_570_residues
MRVIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAINIAGSLRMQSYRLGYDLQRQSPQLHDHRQLFQQAL
HSPVLNNLNAWYVPQAVKTRYAHLHANWLEMNKRLNEGDIDWYQENINNYVNQIDLFVLALQHYAERKMMLVVAISLAGG
IGIFTLVFFTLRRIRHQVVQPLNHLVMASQRMEQGQFDSLPLDTSLPNELGLLAKTFTQMSSELQKLYLSLEASVAEKTR
DLHEAKRRLEVLYQCSQALNTSQIDVHCFRLILQIVREHDAACWLELTVGDNWRISEGHKNGELPLQIIPVTMQDTVYGE
LQWQSENASAFAPLMNSVSTMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIPQDNDTA
QSIMADFSRALNDAYQQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAKLSLDCRLPTLALDAQMQVHLLQIIREAV
VNAIKHANASEIAVSCVTAPDGNHTVFIRDNGIGIGDPTEPEGHYGLNIMRERAERLGGTLSFSQPSGGGTLVSISFRSV
EDEQNHPSGA

Specific function: Acts as a sensor for nitrate/nitrite and transduces signal of nitrate/nitrite availability to the narL/narP proteins. NarQ probably activates narL and narP by phosphorylation. NarQ probably negatively regulates the narL protein by dephosphorylation [H]

COG id: COG3850

COG function: function code T; Signal transduction histidine kinase, nitrate/nitrite-specific

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788812, Length=563, Percent_Identity=88.2770870337478, Blast_Score=1021, Evalue=0.0,
Organism=Escherichia coli, GI1787474, Length=589, Percent_Identity=28.3531409168082, Blast_Score=185, Evalue=7e-48,
Organism=Escherichia coli, GI87082311, Length=212, Percent_Identity=32.0754716981132, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR016380
- InterPro:   IPR011712
- InterPro:   IPR005467 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 64300; Mature: 64300

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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EEECCCCCCCEEEEEEEECCCCCCCCCCCC
>Mature Secondary Structure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EEECCCCCCCEEEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1528845; 1508040; 9205837; 9278503 [H]