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The map label for this gene is hyfB [H]

Identifier: 218548084

GI number: 218548084

Start: 710420

End: 712495

Strand: Reverse

Name: hyfB [H]

Synonym: EFER_0693

Alternate gene names: 218548084

Gene position: 712495-710420 (Counterclockwise)

Preceding gene: 218548085

Following gene: 218548083

Centisome position: 15.53

GC content: 51.78

Gene sequence:

>2076_bases
GTGTCTGGCAGACACAGGGTTGGATTCATTTTCTTCTACTTCGGAGCAAGGCTGATGGACGCTCTGCAATTATTGTTGTG
TTCACTGATGCTATATTTCTTTGCCGGAATCGCGTCGCTGTTCCTTTACGACCTGGATCGCATTGCCATAAAAATTTCCG
GCATCGCATCCCTGGCCGGTGGACTGGTCGGTATTGTCAGCGCTCTGACGCAGCTGCACTCTGCTATTCCGCTGGTTACA
GAATTTATCACGCCATTCCCCTTCGCCACACTCACTATTCGTATGGATAGCCTTTCAGCTTTTATGCTGCTGGTGATTTC
CGTACTGGTCGCGGTGTGTGCTCTCTATTCTCTGGCCTATATGCGTGAATATCTTGGTAAAGGAGCGGCAGCAATCGGCT
TCTTTATGAATATGTTTATCGCCTCTATGGTGAGTTTGCTGGTGATGGATAACGCCTTCTGGTTCATCGTGTTCTTCGAG
ATGATGTCTCTTGCCTCCTGGTTTCTGGTTATCGCCGCTCAGGATAAAGAGTCTATCCGCGCCGGGATGCTTTACTTTTT
TATTGCCCATGCGGGTTCGGTATTGATCATGATCGCCTTCTTCCTGATGTGGCGTGAGTGTCATAGTCTCGACTTCGCCA
GTTTCCGAACGCTACCGCTTTCACCTGGTCTGGCCTCAGCGGTATTTCTGTTGGGTTTCTTCGGGTTTGGCGCCAAAGCC
GGGATGCTCCCTTTGCATAGTTGGCTTCCCCTCGCTCACCCTGCAGCTCCGTCACATGCTTCGGCATTAATGTCGGGAGT
AATGGTAAAAATTGGGATTTTCGGCATTTTGAAAGTCTCCATCGACTTGCTGGGTAATAGCGGTTTGCAATTGTGGTGGG
GCGTGGTGGTGATCATTTTTGGCGCAGTTTCGGCATTATTAGGTGTCCTGTATGCCCTGGCGGAAAATGATATCAAGCGT
CTGCTGGCATGGAGTACCGTGGAAAATGTCGGGATAATCCTCCTCGGTGTGGGGGTGAGTATGGTCGGGCTGTCATTACA
ATTGCCTGTCATCGCCACGGTTGGGTTACTGGCTGCACTGTTTCATCTGCTTAACCACGCCATGTTTAAAGGCTTGCTGT
TTCTCGGTGCGGGGGCTGTTATCGGGCGTCTACACACCCGCGATATGGAAAAAATGGGCGCTCTGGCAAAACGGATGCCA
CGTACCGCAGCTGCTTTTTTGATTGGATGTGTGGCGATTTCCGCCCTGCCGCCATTAAATGGATTTATCAGTGAGTGGTA
TACCTACCAGTCACTGTTCGCCATGACGCATTTTGATGCGGTCACCCTGCGTTTGCTTGGACCTGTTGCCATTGTGATGC
TGGCTGTTACGGGTGCACTGGCAGCGATGTGTTTTGTCAAATTGTATGGCATTAGTTTTTGCGGTGCGCCACGCAGTAGC
GCGGCAGAAGAAGCCCACGAAGCCCCCTGGCAGATGACCGTTTCGTTATTGATCCTCGCGGCACTTTGCATTTTGCTCGG
GGTTGGTGCTCGTTGGGTTGCACCGCACATCTTGCAGGTGGTGTTCTCATTTACGCACCTCACATCGCTTCCCGTTGCAG
AAAGTTTTGCGCTCCTTCCAGGTACCAGTGATGCAACATTACTCACGCCTTCCGCCCTCTTCCTGTTGCTGATTACTGTA
CCGCTTATTCCGGCGCTCTACTGGTGGCTGACCCGTTCTCATCGTGCGGCATTTCGTCGTAGTGGTGATGCATGGGCCTG
TGGTTATGGCTGGGAAAGCGCAATGGCGCCATCAGCCAGCGGTGTTATGCAACCGATGCGTGTGTTTTTCTCTTCACTGT
ACAGATTGCGTAATCAGCTTGATCCCAGCGCGTCACTTGCCTGTGGTTTACAGCACGTGACAGAGGGAGCACGGCGTACA
GAGCCGTTCTGGGACGATTATCTGATTCGCCCCGTAGTAAACGTGATTCAACGTATTGCACTTCGCGTTCAGCAGCTGCA
AAGCGGCGATTTCCGCCTCTATTGTCTGTACGTGGTAGTTGCGTTGGTCGTACTGCTCATCGCTATAGCCCTCTGA

Upstream 100 bases:

>100_bases
GCCCCGCCTGTATTCGAGCTTGTGCCACTAACGCCCTGCAACTCGTCACAGATAAAGTTTTGCATCAGCAAATGAAGCAA
AAACAGCATCTTGCCGCCAC

Downstream 100 bases:

>100_bases
GGAGATCATCATGGCTAACTTTTATAACGACGGGTGTCTGATCGTTTTTGCGCTACTCCAGGCGTTAATTATGCTGGCGC
TGACGCCGTTGTTTACTGGT

Product: hydrogenase 4 subunit B

Products: H2; oxidized ferredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 691; Mature: 690

Protein sequence:

>691_residues
MSGRHRVGFIFFYFGARLMDALQLLLCSLMLYFFAGIASLFLYDLDRIAIKISGIASLAGGLVGIVSALTQLHSAIPLVT
EFITPFPFATLTIRMDSLSAFMLLVISVLVAVCALYSLAYMREYLGKGAAAIGFFMNMFIASMVSLLVMDNAFWFIVFFE
MMSLASWFLVIAAQDKESIRAGMLYFFIAHAGSVLIMIAFFLMWRECHSLDFASFRTLPLSPGLASAVFLLGFFGFGAKA
GMLPLHSWLPLAHPAAPSHASALMSGVMVKIGIFGILKVSIDLLGNSGLQLWWGVVVIIFGAVSALLGVLYALAENDIKR
LLAWSTVENVGIILLGVGVSMVGLSLQLPVIATVGLLAALFHLLNHAMFKGLLFLGAGAVIGRLHTRDMEKMGALAKRMP
RTAAAFLIGCVAISALPPLNGFISEWYTYQSLFAMTHFDAVTLRLLGPVAIVMLAVTGALAAMCFVKLYGISFCGAPRSS
AAEEAHEAPWQMTVSLLILAALCILLGVGARWVAPHILQVVFSFTHLTSLPVAESFALLPGTSDATLLTPSALFLLLITV
PLIPALYWWLTRSHRAAFRRSGDAWACGYGWESAMAPSASGVMQPMRVFFSSLYRLRNQLDPSASLACGLQHVTEGARRT
EPFWDDYLIRPVVNVIQRIALRVQQLQSGDFRLYCLYVVVALVVLLIAIAL

Sequences:

>Translated_691_residues
MSGRHRVGFIFFYFGARLMDALQLLLCSLMLYFFAGIASLFLYDLDRIAIKISGIASLAGGLVGIVSALTQLHSAIPLVT
EFITPFPFATLTIRMDSLSAFMLLVISVLVAVCALYSLAYMREYLGKGAAAIGFFMNMFIASMVSLLVMDNAFWFIVFFE
MMSLASWFLVIAAQDKESIRAGMLYFFIAHAGSVLIMIAFFLMWRECHSLDFASFRTLPLSPGLASAVFLLGFFGFGAKA
GMLPLHSWLPLAHPAAPSHASALMSGVMVKIGIFGILKVSIDLLGNSGLQLWWGVVVIIFGAVSALLGVLYALAENDIKR
LLAWSTVENVGIILLGVGVSMVGLSLQLPVIATVGLLAALFHLLNHAMFKGLLFLGAGAVIGRLHTRDMEKMGALAKRMP
RTAAAFLIGCVAISALPPLNGFISEWYTYQSLFAMTHFDAVTLRLLGPVAIVMLAVTGALAAMCFVKLYGISFCGAPRSS
AAEEAHEAPWQMTVSLLILAALCILLGVGARWVAPHILQVVFSFTHLTSLPVAESFALLPGTSDATLLTPSALFLLLITV
PLIPALYWWLTRSHRAAFRRSGDAWACGYGWESAMAPSASGVMQPMRVFFSSLYRLRNQLDPSASLACGLQHVTEGARRT
EPFWDDYLIRPVVNVIQRIALRVQQLQSGDFRLYCLYVVVALVVLLIAIAL
>Mature_690_residues
SGRHRVGFIFFYFGARLMDALQLLLCSLMLYFFAGIASLFLYDLDRIAIKISGIASLAGGLVGIVSALTQLHSAIPLVTE
FITPFPFATLTIRMDSLSAFMLLVISVLVAVCALYSLAYMREYLGKGAAAIGFFMNMFIASMVSLLVMDNAFWFIVFFEM
MSLASWFLVIAAQDKESIRAGMLYFFIAHAGSVLIMIAFFLMWRECHSLDFASFRTLPLSPGLASAVFLLGFFGFGAKAG
MLPLHSWLPLAHPAAPSHASALMSGVMVKIGIFGILKVSIDLLGNSGLQLWWGVVVIIFGAVSALLGVLYALAENDIKRL
LAWSTVENVGIILLGVGVSMVGLSLQLPVIATVGLLAALFHLLNHAMFKGLLFLGAGAVIGRLHTRDMEKMGALAKRMPR
TAAAFLIGCVAISALPPLNGFISEWYTYQSLFAMTHFDAVTLRLLGPVAIVMLAVTGALAAMCFVKLYGISFCGAPRSSA
AEEAHEAPWQMTVSLLILAALCILLGVGARWVAPHILQVVFSFTHLTSLPVAESFALLPGTSDATLLTPSALFLLLITVP
LIPALYWWLTRSHRAAFRRSGDAWACGYGWESAMAPSASGVMQPMRVFFSSLYRLRNQLDPSASLACGLQHVTEGARRTE
PFWDDYLIRPVVNVIQRIALRVQQLQSGDFRLYCLYVVVALVVLLIAIAL

Specific function: Unknown

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=357, Percent_Identity=28.5714285714286, Blast_Score=108, Evalue=2e-23,
Organism=Escherichia coli, GI1788827, Length=673, Percent_Identity=73.4026745913819, Blast_Score=932, Evalue=0.0,
Organism=Escherichia coli, GI2367154, Length=618, Percent_Identity=36.8932038834951, Blast_Score=334, Evalue=1e-92,
Organism=Escherichia coli, GI1788831, Length=372, Percent_Identity=30.9139784946237, Blast_Score=155, Evalue=7e-39,
Organism=Escherichia coli, GI1788829, Length=425, Percent_Identity=26.8235294117647, Blast_Score=129, Evalue=5e-31,
Organism=Escherichia coli, GI1788614, Length=367, Percent_Identity=32.6975476839237, Blast_Score=126, Evalue=6e-30,
Organism=Escherichia coli, GI1788613, Length=380, Percent_Identity=26.8421052631579, Blast_Score=120, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 75088; Mature: 74957

Theoretical pI: Translated: 8.78; Mature: 8.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHCCCCEEHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
SGRHRVGFIFFYFGARLMDALQLLLCSLMLYFFAGIASLFLYDLDRIAIKISGIASLAG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH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HHHHHHCCCCEEHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 2016588 [H]