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The map label for this gene is hyfC [H]

Identifier: 218548083

GI number: 218548083

Start: 709462

End: 710409

Strand: Reverse

Name: hyfC [H]

Synonym: EFER_0692

Alternate gene names: 218548083

Gene position: 710409-709462 (Counterclockwise)

Preceding gene: 218548084

Following gene: 218548082

Centisome position: 15.48

GC content: 52.53

Gene sequence:

>948_bases
ATGGCTAACTTTTATAACGACGGGTGTCTGATCGTTTTTGCGCTACTCCAGGCGTTAATTATGCTGGCGCTGACGCCGTT
GTTTACTGGTATTTCCCGCCAGATCCGTGCCTGGATGCATTCTCGTCGTGGGCCAGACATTTGGCAAGATTATCGTGATA
TCAACAAGTTACTAAAACGCCAGGAAGTTGCTCCGTCCTCCTCAGGTTTAATGTTTCGCCTGATGCCCTGGGTATTACTC
AGTAGCATGATGGTCGTTGCCATGACCTTGCCGTTGTTTTTACGAGAATCACCTTTCGCTGGGGGCGGCGACATGATCAC
GTTGATTTATCTGCTCGCCCTTTATCGTTTCTTTTTTGCCCTTTCCGGGTTGGATACCGGAAGTCCCTTCGCGGGTGTTG
GTGCCTCAAGGGAGTTAGCACTGGGCATTTTGGTGGAACCGATTCTGATCCTCGCCCTGTTGGTGCTGGCACTCATTGCC
GGTTCCACGCACATCGCCGTGATCAGTCATACGCTGGCAGGCGGTTGGATTTCACCACTGGCAACGTTGCTGGCACTTCT
GGCGTGTGTTTTCGCCTGCTTCATTGAAATGGGCAAAATTCCGTTTGATGTTGCCGAAGCTGAGCAGGAGTTACAGGAGG
GGCCGCTAACAGAGTATTCCGGTGCCGGGCTGGCACTGGTGAAATGGGGGTTAGGGCTTAAACAGGTGGTGATGGCGGCG
CTCGTTGTCTCGCTGTTTGTACCTTTTGGCAACGCCCCGGATAGCGCCCCGGCTGACCTGTTGCTCTCTTTGATTCTCTC
TCTGGGCAAGATTCTGCTGGTTTTTATTCTGGCTTCCATTGTGGAGAACTCACTGGCACGTGGACGCTTTTTGCTCACAC
ACCATTTGACCTGGCTCGGTTTTAGTCTCGCCGCGCTGTCGTATGTCTTTTGGTTAACCGGACTGTAG

Upstream 100 bases:

>100_bases
GTTCAGCAGCTGCAAAGCGGCGATTTCCGCCTCTATTGTCTGTACGTGGTAGTTGCGTTGGTCGTACTGCTCATCGCTAT
AGCCCTCTGAGGAGATCATC

Downstream 100 bases:

>100_bases
GGAGGCTTGCAGCCAATGGAAAATCTTGCTTTAACCACGCTCTTGCTACCCTTTATTGGCGCCCTGGTTGTTTCGCTAAG
CCCGCAACGTCATGCCGCTA

Product: hydrogenase 4, membrane subunit

Products: H2; oxidized ferredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MANFYNDGCLIVFALLQALIMLALTPLFTGISRQIRAWMHSRRGPDIWQDYRDINKLLKRQEVAPSSSGLMFRLMPWVLL
SSMMVVAMTLPLFLRESPFAGGGDMITLIYLLALYRFFFALSGLDTGSPFAGVGASRELALGILVEPILILALLVLALIA
GSTHIAVISHTLAGGWISPLATLLALLACVFACFIEMGKIPFDVAEAEQELQEGPLTEYSGAGLALVKWGLGLKQVVMAA
LVVSLFVPFGNAPDSAPADLLLSLILSLGKILLVFILASIVENSLARGRFLLTHHLTWLGFSLAALSYVFWLTGL

Sequences:

>Translated_315_residues
MANFYNDGCLIVFALLQALIMLALTPLFTGISRQIRAWMHSRRGPDIWQDYRDINKLLKRQEVAPSSSGLMFRLMPWVLL
SSMMVVAMTLPLFLRESPFAGGGDMITLIYLLALYRFFFALSGLDTGSPFAGVGASRELALGILVEPILILALLVLALIA
GSTHIAVISHTLAGGWISPLATLLALLACVFACFIEMGKIPFDVAEAEQELQEGPLTEYSGAGLALVKWGLGLKQVVMAA
LVVSLFVPFGNAPDSAPADLLLSLILSLGKILLVFILASIVENSLARGRFLLTHHLTWLGFSLAALSYVFWLTGL
>Mature_314_residues
ANFYNDGCLIVFALLQALIMLALTPLFTGISRQIRAWMHSRRGPDIWQDYRDINKLLKRQEVAPSSSGLMFRLMPWVLLS
SMMVVAMTLPLFLRESPFAGGGDMITLIYLLALYRFFFALSGLDTGSPFAGVGASRELALGILVEPILILALLVLALIAG
STHIAVISHTLAGGWISPLATLLALLACVFACFIEMGKIPFDVAEAEQELQEGPLTEYSGAGLALVKWGLGLKQVVMAAL
VVSLFVPFGNAPDSAPADLLLSLILSLGKILLVFILASIVENSLARGRFLLTHHLTWLGFSLAALSYVFWLTGL

Specific function: Unknown

COG id: COG0650

COG function: function code C; Formate hydrogenlyase subunit 4

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 1 family [H]

Homologues:

Organism=Escherichia coli, GI87082115, Length=315, Percent_Identity=80, Blast_Score=460, Evalue=1e-131,
Organism=Escherichia coli, GI1789077, Length=300, Percent_Identity=50.3333333333333, Blast_Score=299, Evalue=2e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001694
- InterPro:   IPR018086 [H]

Pfam domain/function: PF00146 NADHdh [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 34137; Mature: 34006

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS00668 COMPLEX1_ND1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANFYNDGCLIVFALLQALIMLALTPLFTGISRQIRAWMHSRRGPDIWQDYRDINKLLKR
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
QEVAPSSSGLMFRLMPWVLLSSMMVVAMTLPLFLRESPFAGGGDMITLIYLLALYRFFFA
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
LSGLDTGSPFAGVGASRELALGILVEPILILALLVLALIAGSTHIAVISHTLAGGWISPL
HHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHH
ATLLALLACVFACFIEMGKIPFDVAEAEQELQEGPLTEYSGAGLALVKWGLGLKQVVMAA
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCHHHHHHHH
LVVSLFVPFGNAPDSAPADLLLSLILSLGKILLVFILASIVENSLARGRFLLTHHLTWLG
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHH
FSLAALSYVFWLTGL
HHHHHHHHHHHHHCC
>Mature Secondary Structure 
ANFYNDGCLIVFALLQALIMLALTPLFTGISRQIRAWMHSRRGPDIWQDYRDINKLLKR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
QEVAPSSSGLMFRLMPWVLLSSMMVVAMTLPLFLRESPFAGGGDMITLIYLLALYRFFFA
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
LSGLDTGSPFAGVGASRELALGILVEPILILALLVLALIAGSTHIAVISHTLAGGWISPL
HHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHH
ATLLALLACVFACFIEMGKIPFDVAEAEQELQEGPLTEYSGAGLALVKWGLGLKQVVMAA
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCHHHHHHHH
LVVSLFVPFGNAPDSAPADLLLSLILSLGKILLVFILASIVENSLARGRFLLTHHLTWLG
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHH
FSLAALSYVFWLTGL
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]