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The map label for this gene is hyfA [H]

Identifier: 218548085

GI number: 218548085

Start: 712441

End: 713058

Strand: Reverse

Name: hyfA [H]

Synonym: EFER_0694

Alternate gene names: 218548085

Gene position: 713058-712441 (Counterclockwise)

Preceding gene: 218548086

Following gene: 218548084

Centisome position: 15.54

GC content: 52.75

Gene sequence:

>618_bases
ATGAACCGCTTTGTAATGGCCGATCCTCAGTGGTGTACAGGATGTAAAACCTGTCTCGCTGCGTGTTCGGACGTACATAA
AAAGCAAGGCTTACAGCAACATCCTCGACTCGCCCTGGTTTGCACACCTGAGCAAACTGCGCCTGTTCAGTGCCATCACT
GTGAGGATGCGCCCTGTCAGCAGGTCTGCCCGGTTAACGCAATTTCCCGGCATGACGACGCAATCCAGCTCAATGAAACG
CTCTGCATTGGCTGCAAACTCTGTGCATTGGTATGTCCGTTTGGCGCGATAACGGCTGCAGGAAGTGGACCAGTGGACGC
GCCAGCGTTATATCAGCACCAGGCTGAGGAGCCACTTAACGATATCCCGGAAAGCTCACCGACATTGCATCCCTTATTAC
GCTGGCAGGTTGGCGTCCAGGCAGTCGCTGTGAAATGTGATCTCTGTTATTTCCTGCCAGAAGGCCCCGCCTGTATTCGA
GCTTGTGCCACTAACGCCCTGCAACTCGTCACAGATAAAGTTTTGCATCAGCAAATGAAGCAAAAACAGCATCTTGCCGC
CACGTGTCTGGCAGACACAGGGTTGGATTCATTTTCTTCTACTTCGGAGCAAGGCTGA

Upstream 100 bases:

>100_bases
GTTGAAAATCATATAACAAACTGAAATAAAAGCACTTTTAATGATGGCATCCTTTATGCATAGCAAGGCGTAACGTTTTG
TTTTTATTTAAGGAGCCGAC

Downstream 100 bases:

>100_bases
TGGACGCTCTGCAATTATTGTTGTGTTCACTGATGCTATATTTCTTTGCCGGAATCGCGTCGCTGTTCCTTTACGACCTG
GATCGCATTGCCATAAAAAT

Product: hydrogenase 4, 4Fe-4S subunit

Products: H2; oxidized ferredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 205; Mature: 205

Protein sequence:

>205_residues
MNRFVMADPQWCTGCKTCLAACSDVHKKQGLQQHPRLALVCTPEQTAPVQCHHCEDAPCQQVCPVNAISRHDDAIQLNET
LCIGCKLCALVCPFGAITAAGSGPVDAPALYQHQAEEPLNDIPESSPTLHPLLRWQVGVQAVAVKCDLCYFLPEGPACIR
ACATNALQLVTDKVLHQQMKQKQHLAATCLADTGLDSFSSTSEQG

Sequences:

>Translated_205_residues
MNRFVMADPQWCTGCKTCLAACSDVHKKQGLQQHPRLALVCTPEQTAPVQCHHCEDAPCQQVCPVNAISRHDDAIQLNET
LCIGCKLCALVCPFGAITAAGSGPVDAPALYQHQAEEPLNDIPESSPTLHPLLRWQVGVQAVAVKCDLCYFLPEGPACIR
ACATNALQLVTDKVLHQQMKQKQHLAATCLADTGLDSFSSTSEQG
>Mature_205_residues
MNRFVMADPQWCTGCKTCLAACSDVHKKQGLQQHPRLALVCTPEQTAPVQCHHCEDAPCQQVCPVNAISRHDDAIQLNET
LCIGCKLCALVCPFGAITAAGSGPVDAPALYQHQAEEPLNDIPESSPTLHPLLRWQVGVQAVAVKCDLCYFLPEGPACIR
ACATNALQLVTDKVLHQQMKQKQHLAATCLADTGLDSFSSTSEQG

Specific function: Probable electron transfer protein for hydrogenase 4 [H]

COG id: COG1142

COG function: function code C; Fe-S-cluster-containing hydrogenase components 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI87082114, Length=204, Percent_Identity=70.0980392156863, Blast_Score=287, Evalue=3e-79,
Organism=Escherichia coli, GI1789079, Length=185, Percent_Identity=51.8918918918919, Blast_Score=189, Evalue=1e-49,
Organism=Escherichia coli, GI1788811, Length=188, Percent_Identity=39.8936170212766, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI2367245, Length=190, Percent_Identity=35.7894736842105, Blast_Score=107, Evalue=6e-25,
Organism=Escherichia coli, GI87082180, Length=199, Percent_Identity=33.1658291457286, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI87082179, Length=186, Percent_Identity=34.9462365591398, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1789067, Length=194, Percent_Identity=34.5360824742268, Blast_Score=96, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 22074; Mature: 22074

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

8.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
10.2 %Cys+Met (Translated Protein)
8.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
10.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRFVMADPQWCTGCKTCLAACSDVHKKQGLQQHPRLALVCTPEQTAPVQCHHCEDAPCQ
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHCCCEEEEECCCCCCCEEEECCCCCCHH
QVCPVNAISRHDDAIQLNETLCIGCKLCALVCPFGAITAAGSGPVDAPALYQHQAEEPLN
HCCCCCHHHCCCCCEEECCHHHHHHHHHHHHCCHHHHCCCCCCCCCCHHHHHHHHCCCHH
DIPESSPTLHPLLRWQVGVQAVAVKCDLCYFLPEGPACIRACATNALQLVTDKVLHQQMK
HCCCCCCCHHHHHHHHHHHHHEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QKQHLAATCLADTGLDSFSSTSEQG
HHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MNRFVMADPQWCTGCKTCLAACSDVHKKQGLQQHPRLALVCTPEQTAPVQCHHCEDAPCQ
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHCCCEEEEECCCCCCCEEEECCCCCCHH
QVCPVNAISRHDDAIQLNETLCIGCKLCALVCPFGAITAAGSGPVDAPALYQHQAEEPLN
HCCCCCHHHCCCCCEEECCHHHHHHHHHHHHCCHHHHCCCCCCCCCCHHHHHHHHCCCHH
DIPESSPTLHPLLRWQVGVQAVAVKCDLCYFLPEGPACIRACATNALQLVTDKVLHQQMK
HCCCCCCCHHHHHHHHHHHHHEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QKQHLAATCLADTGLDSFSSTSEQG
HHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2016588; 9205837; 9278503 [H]