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The map label for this gene is hyfD [H]

Identifier: 218548082

GI number: 218548082

Start: 708007

End: 709455

Strand: Reverse

Name: hyfD [H]

Synonym: EFER_0691

Alternate gene names: 218548082

Gene position: 709455-708007 (Counterclockwise)

Preceding gene: 218548083

Following gene: 218548081

Centisome position: 15.46

GC content: 49.9

Gene sequence:

>1449_bases
TTGCAGCCAATGGAAAATCTTGCTTTAACCACGCTCTTGCTACCCTTTATTGGCGCCCTGGTTGTTTCGCTAAGCCCGCA
ACGTCATGCCGCTAAACTCGGTACGCTGTTTGCCTCGCTCACAACGATTTGTTTGATTTCGCTGGAAGCCGCTTTTTATC
AGAGCGGAAAAAGTGCGGTCACGCTGCCACTGGCACAGGTTGGTGATGTGGCACTGTTTGGGCTGGTCATTGACAAAGTA
AGTAGCCTGGTGTTGTTTGTGGTGGTCTTTCTCGGCCTGCTGGTCACCATTTACTCAACCGGTTATCTGACCGATAAAAA
CCGTGAACATCCACACCAGGGTAGCAACCGCTATTACGCTTTTTTGCTGGTGTTTATCGGCGCGATGTCAGGTCTGGTAC
TCTCATCAACAATTCTCGGTCAGTTGCTATTCTTCGAAATTACAGGCGGATGTTCCTGGGCGTTAATTGGTTATTACCAG
AGTGACAAAGCACAGCGTTCTGCAATCAAAGCCCTGCTAATGACTCACGTCGGATCACTGGGGCTTTATATCGCCGCTGC
GACACTTTTCCTGCACACCGGTACATTTGCACTCGCTGCACTCAGTGATATTCATGGTGATGCTCGTTACCTGGTCTATG
GCGGCATTCTCTTTGCCGCATGGGGTAAATCAGCACAGTTACCCCTTCAGGCCTGGCTGCCAGATGCCATGGAAGCCCCC
ACACCGATCAGTGCTTATCTACATGCTGCTTCAATGGTGAAAGTTGGGGTTTATATCTATGCCCGTGCGATCCTTGATGG
CGGCAATATTCCCATTGAAATTGGCATGATTGGTCTGGTGATGGCACTTGCCACCATCATTTACGGGTTCATGATGTATC
TGCCGCAACAGGATATGAAACGTCTGCTGGCATGGTCGACAATTACCCAATTAGGCTGGATGTTCTTCGGGCTTTCGCTG
TCTATTTTTGGCTCCCCACTGGCGTTTATCGGCAGTATTGCCTACCTCGTTAACCACGCTTTCGCTAAGAGTCTCTTTTT
CTTAATCGCAGGGTCGCTCAGTTTTAGCTGTGGTACTCGCATGCTTCCCAAACTGCGTGGTGTTTTGCGGATACTTCCCC
TTGCTGGTATTGGATTCTGTGTCGCTGCGCTAGCGATTACAGGTGTTCCGCCATTTAACGGTTTCTTTAGTAAGTATCCG
CTGTTTGCCGCCGGCTTTGCGCTTTCCAGTATTTATCCCGCGCTACTCATCGCCATGATTCTGCTGTTAATCGAATCAGT
CGCCAGTTTTGGCTGGTTCATTTACTGGTTTGGACGCGTCGTACCAGGGAAGCCCAGTGACGAAGTTGCGCACGCTTCTC
CGTTGCCGTTTTCTATGCGTCTGGTGCTGATTGTGCTCATTGTGATGTCGCTTATCTCCAGCGTAATCGCTGCTGTATGG
CTTCAGTAA

Upstream 100 bases:

>100_bases
GGCACGTGGACGCTTTTTGCTCACACACCATTTGACCTGGCTCGGTTTTAGTCTCGCCGCGCTGTCGTATGTCTTTTGGT
TAACCGGACTGTAGGGAGGC

Downstream 100 bases:

>100_bases
GGAGTTGAAATATGACGGGTATATCAATTGTTAATAACCTGGCAGGACTCATGATGCTGACTTCACTGTTCGTGATCAGC
GTGAAAGGCTTTCGCCTTGC

Product: hydrogenase 4 subunit D

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MQPMENLALTTLLLPFIGALVVSLSPQRHAAKLGTLFASLTTICLISLEAAFYQSGKSAVTLPLAQVGDVALFGLVIDKV
SSLVLFVVVFLGLLVTIYSTGYLTDKNREHPHQGSNRYYAFLLVFIGAMSGLVLSSTILGQLLFFEITGGCSWALIGYYQ
SDKAQRSAIKALLMTHVGSLGLYIAAATLFLHTGTFALAALSDIHGDARYLVYGGILFAAWGKSAQLPLQAWLPDAMEAP
TPISAYLHAASMVKVGVYIYARAILDGGNIPIEIGMIGLVMALATIIYGFMMYLPQQDMKRLLAWSTITQLGWMFFGLSL
SIFGSPLAFIGSIAYLVNHAFAKSLFFLIAGSLSFSCGTRMLPKLRGVLRILPLAGIGFCVAALAITGVPPFNGFFSKYP
LFAAGFALSSIYPALLIAMILLLIESVASFGWFIYWFGRVVPGKPSDEVAHASPLPFSMRLVLIVLIVMSLISSVIAAVW
LQ

Sequences:

>Translated_482_residues
MQPMENLALTTLLLPFIGALVVSLSPQRHAAKLGTLFASLTTICLISLEAAFYQSGKSAVTLPLAQVGDVALFGLVIDKV
SSLVLFVVVFLGLLVTIYSTGYLTDKNREHPHQGSNRYYAFLLVFIGAMSGLVLSSTILGQLLFFEITGGCSWALIGYYQ
SDKAQRSAIKALLMTHVGSLGLYIAAATLFLHTGTFALAALSDIHGDARYLVYGGILFAAWGKSAQLPLQAWLPDAMEAP
TPISAYLHAASMVKVGVYIYARAILDGGNIPIEIGMIGLVMALATIIYGFMMYLPQQDMKRLLAWSTITQLGWMFFGLSL
SIFGSPLAFIGSIAYLVNHAFAKSLFFLIAGSLSFSCGTRMLPKLRGVLRILPLAGIGFCVAALAITGVPPFNGFFSKYP
LFAAGFALSSIYPALLIAMILLLIESVASFGWFIYWFGRVVPGKPSDEVAHASPLPFSMRLVLIVLIVMSLISSVIAAVW
LQ
>Mature_482_residues
MQPMENLALTTLLLPFIGALVVSLSPQRHAAKLGTLFASLTTICLISLEAAFYQSGKSAVTLPLAQVGDVALFGLVIDKV
SSLVLFVVVFLGLLVTIYSTGYLTDKNREHPHQGSNRYYAFLLVFIGAMSGLVLSSTILGQLLFFEITGGCSWALIGYYQ
SDKAQRSAIKALLMTHVGSLGLYIAAATLFLHTGTFALAALSDIHGDARYLVYGGILFAAWGKSAQLPLQAWLPDAMEAP
TPISAYLHAASMVKVGVYIYARAILDGGNIPIEIGMIGLVMALATIIYGFMMYLPQQDMKRLLAWSTITQLGWMFFGLSL
SIFGSPLAFIGSIAYLVNHAFAKSLFFLIAGSLSFSCGTRMLPKLRGVLRILPLAGIGFCVAALAITGVPPFNGFFSKYP
LFAAGFALSSIYPALLIAMILLLIESVASFGWFIYWFGRVVPGKPSDEVAHASPLPFSMRLVLIVLIVMSLISSVIAAVW
LQ

Specific function: Unknown

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=285, Percent_Identity=32.280701754386, Blast_Score=134, Evalue=2e-31,
Organism=Escherichia coli, GI1788829, Length=479, Percent_Identity=84.7599164926931, Blast_Score=814, Evalue=0.0,
Organism=Escherichia coli, GI1788614, Length=425, Percent_Identity=33.6470588235294, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1788827, Length=437, Percent_Identity=25.858123569794, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1788831, Length=354, Percent_Identity=30.5084745762712, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI2367154, Length=269, Percent_Identity=29.368029739777, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1788613, Length=412, Percent_Identity=27.1844660194175, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI145693160, Length=312, Percent_Identity=25.9615384615385, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR001516 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF00662 Oxidored_q1_N [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 51872; Mature: 51872

Theoretical pI: Translated: 9.26; Mature: 9.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPMENLALTTLLLPFIGALVVSLSPQRHAAKLGTLFASLTTICLISLEAAFYQSGKSAV
CCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
TLPLAQVGDVALFGLVIDKVSSLVLFVVVFLGLLVTIYSTGYLTDKNREHPHQGSNRYYA
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHH
FLLVFIGAMSGLVLSSTILGQLLFFEITGGCSWALIGYYQSDKAQRSAIKALLMTHVGSL
HHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEEEEHHCCHHHHHHHHHHHHHHHHHH
GLYIAAATLFLHTGTFALAALSDIHGDARYLVYGGILFAAWGKSAQLPLQAWLPDAMEAP
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCCCCCHHHCCCHHHCCC
TPISAYLHAASMVKVGVYIYARAILDGGNIPIEIGMIGLVMALATIIYGFMMYLPQQDMK
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCHHHHH
RLLAWSTITQLGWMFFGLSLSIFGSPLAFIGSIAYLVNHAFAKSLFFLIAGSLSFSCGTR
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
MLPKLRGVLRILPLAGIGFCVAALAITGVPPFNGFFSKYPLFAAGFALSSIYPALLIAMI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHH
LLLIESVASFGWFIYWFGRVVPGKPSDEVAHASPLPFSMRLVLIVLIVMSLISSVIAAVW
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LQ
CC
>Mature Secondary Structure
MQPMENLALTTLLLPFIGALVVSLSPQRHAAKLGTLFASLTTICLISLEAAFYQSGKSAV
CCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
TLPLAQVGDVALFGLVIDKVSSLVLFVVVFLGLLVTIYSTGYLTDKNREHPHQGSNRYYA
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHH
FLLVFIGAMSGLVLSSTILGQLLFFEITGGCSWALIGYYQSDKAQRSAIKALLMTHVGSL
HHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEEEEHHCCHHHHHHHHHHHHHHHHHH
GLYIAAATLFLHTGTFALAALSDIHGDARYLVYGGILFAAWGKSAQLPLQAWLPDAMEAP
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCCCCCHHHCCCHHHCCC
TPISAYLHAASMVKVGVYIYARAILDGGNIPIEIGMIGLVMALATIIYGFMMYLPQQDMK
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCHHHHH
RLLAWSTITQLGWMFFGLSLSIFGSPLAFIGSIAYLVNHAFAKSLFFLIAGSLSFSCGTR
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
MLPKLRGVLRILPLAGIGFCVAALAITGVPPFNGFFSKYPLFAAGFALSSIYPALLIAMI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHH
LLLIESVASFGWFIYWFGRVVPGKPSDEVAHASPLPFSMRLVLIVLIVMSLISSVIAAVW
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LQ
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]