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The map label for this gene is hyfH [H]

Identifier: 218548078

GI number: 218548078

Start: 703497

End: 704045

Strand: Reverse

Name: hyfH [H]

Synonym: EFER_0687

Alternate gene names: 218548078

Gene position: 704045-703497 (Counterclockwise)

Preceding gene: 218548079

Following gene: 218548077

Centisome position: 15.34

GC content: 51.73

Gene sequence:

>549_bases
ATGCTGAAATTACTCAAAACCATTATGCGCGCCGGTGAAGCAACGGTGAAATATCCTTTCGCGCCGCTGGAAGTTAGCCC
TGGTTTTCGGGGCAAGCCGGAACTGGATGCCGCGCAGTGTATTGCCTGTGCTGCCTGTGCTTCCGCCTGCCCGGCCAATG
CGCTGACCATCCTGACGGATGAACAACAAGATACCCGCACCTGGCAACTTTTTCTTGGTCGCTGCATTTACTGCGGGCGC
TGTGAAGAGGTCTGCCCAACAAGGGCAATCACGTTGTCAGAAAATTTTGAGCTGACCGTAACGAACAAAGCCGATCTTTA
CACCCGTGCAACCTTCCACCTGCAACATTGCAAGCAATGCGGTAAACCTTTTGCCGCGCAGAAATCAGTCGATGTGGCGG
TGAAATTACTCACTCAGCAACTTAACGCTGAACAAAATGAACAATTTTTGCGCCAGCAAGCCAGCATTTGCCCTGGCTGC
AAACAGCACGCTGCGCTGGTTAATGGTTGTGTGAAAAATCTCTCTTCGGGAAATCGGGAGGCACAATGA

Upstream 100 bases:

>100_bases
GGTTGATGTGCGTAAGCATCAGTCAAAAACCGTGCCGTATAAAGAGATCGAGCGCTATGGCATTGATCGTAACCGCTCAC
CGCTGAAGTAAGGACAGAAA

Downstream 100 bases:

>100_bases
GTTCGATGCTAAAAACCCATCGTCAACATGTTAGCCAGCCGATGGTCCAGGATGAGCAAACGCAACAAATGAAACAGCTA
CTGCTCAAAGATATTCGTCG

Product: hydrogenase 4 subunit H

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 182; Mature: 182

Protein sequence:

>182_residues
MLKLLKTIMRAGEATVKYPFAPLEVSPGFRGKPELDAAQCIACAACASACPANALTILTDEQQDTRTWQLFLGRCIYCGR
CEEVCPTRAITLSENFELTVTNKADLYTRATFHLQHCKQCGKPFAAQKSVDVAVKLLTQQLNAEQNEQFLRQQASICPGC
KQHAALVNGCVKNLSSGNREAQ

Sequences:

>Translated_182_residues
MLKLLKTIMRAGEATVKYPFAPLEVSPGFRGKPELDAAQCIACAACASACPANALTILTDEQQDTRTWQLFLGRCIYCGR
CEEVCPTRAITLSENFELTVTNKADLYTRATFHLQHCKQCGKPFAAQKSVDVAVKLLTQQLNAEQNEQFLRQQASICPGC
KQHAALVNGCVKNLSSGNREAQ
>Mature_182_residues
MLKLLKTIMRAGEATVKYPFAPLEVSPGFRGKPELDAAQCIACAACASACPANALTILTDEQQDTRTWQLFLGRCIYCGR
CEEVCPTRAITLSENFELTVTNKADLYTRATFHLQHCKQCGKPFAAQKSVDVAVKLLTQQLNAEQNEQFLRQQASICPGC
KQHAALVNGCVKNLSSGNREAQ

Specific function: Probable electron transfer protein for hydrogenase 4 [H]

COG id: COG1143

COG function: function code C; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI4505371, Length=106, Percent_Identity=39.622641509434, Blast_Score=73, Evalue=2e-13,
Organism=Escherichia coli, GI1788833, Length=168, Percent_Identity=76.1904761904762, Blast_Score=241, Evalue=2e-65,
Organism=Escherichia coli, GI1789075, Length=181, Percent_Identity=43.646408839779, Blast_Score=160, Evalue=3e-41,
Organism=Escherichia coli, GI1788617, Length=105, Percent_Identity=37.1428571428571, Blast_Score=72, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17555194, Length=112, Percent_Identity=37.5, Blast_Score=70, Evalue=6e-13,
Organism=Drosophila melanogaster, GI17864306, Length=106, Percent_Identity=40.5660377358491, Blast_Score=77, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: NA

Molecular weight: Translated: 19955; Mature: 19955

Theoretical pI: Translated: 8.11; Mature: 8.11

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

7.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
8.2 %Cys+Met (Translated Protein)
7.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
8.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKLLKTIMRAGEATVKYPFAPLEVSPGFRGKPELDAAQCIACAACASACPANALTILTD
CHHHHHHHHHCCCCEEECCCCCEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEC
EQQDTRTWQLFLGRCIYCGRCEEVCPTRAITLSENFELTVTNKADLYTRATFHLQHCKQC
CCCHHHHHHHHHHHHHHCCCCHHHCCCCEEEECCCEEEEEECCCCHHHHHHHHHHHHHHH
GKPFAAQKSVDVAVKLLTQQLNAEQNEQFLRQQASICPGCKQHAALVNGCVKNLSSGNRE
CCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCC
AQ
CC
>Mature Secondary Structure
MLKLLKTIMRAGEATVKYPFAPLEVSPGFRGKPELDAAQCIACAACASACPANALTILTD
CHHHHHHHHHCCCCEEECCCCCEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEC
EQQDTRTWQLFLGRCIYCGRCEEVCPTRAITLSENFELTVTNKADLYTRATFHLQHCKQC
CCCHHHHHHHHHHHHHHCCCCHHHCCCCEEEECCCEEEEEECCCCHHHHHHHHHHHHHHH
GKPFAAQKSVDVAVKLLTQQLNAEQNEQFLRQQASICPGCKQHAALVNGCVKNLSSGNRE
CCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCC
AQ
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]